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Series GSE19494 Query DataSets for GSE19494
Status Public on Oct 01, 2010
Title Subcutaneous adipose tissue (SAT) gene expression patterns between weight control and regaining weight subjects
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Objective: This work aimed at identifying and characterizing differences in subcutaneous adipose tissue (SAT) gene expression patterns between subjects succeeding weight control against subjects regaining weight 6 months after a low calorie diet program. Methods: Weight-reduced obese subjects from 8 European countries were randomized into 4 diets differing in protein and glycemic index. In addition to the anthropometric and plasma parameters, SAT biopsies were taken at the beginning (CID2) and the end (CID3) of the weight maintenance intervention. Pan-genomic cDNA microarrays were used to define SAT gene expression profiles at both CID2 and CID3 of 22 subjects succeeding weight maintenance (successful subjects; mean weight change= -2.6 ± 1.2 kg) and 22 subjects regaining weight (unsuccessful subjects; mean weight change= 3.9 ± 1.3 kg) among the 4 dietary arms. Results: Differences in SAT gene expression patterns between successful and unsuccessful groups were mainly due to weight variations rather to differences in diet macronutrient composition. An ANCOVA analysis with total energy intake at CID3 as covariant led us to the definition of 1339 differential genes. Functional analysis of differential genes showed that cellular growth and proliferation, inflammation, cell death, cancer, cellular function and maintenance were the main biological processes involving these differential genes.
 
Overall design Overall design: SAT transcriptome was defined at the beginning (CID2) and at the end (CID3) of the dietary intervention program using a common reference design: Cyanine-5 dye was incorporated into all SAT samples, while a reference RNA pool made of the mix of commercial human liver, adipose tissue, and skeletal muscle RNA was labeled with cyanine-3 dye. Samples were hybridized to Agilent 44K whole human genome microarrays. Evolution in gene expression patterns after dietary intervention was tested in terms of the ratio after/before dietary intervention (CID3/CID2) ratio.
 
Contributor(s) Marquez-Quiñones A, Mutch DM, Wang P, Mariman E, Debard C, Vidal H, Combes M, Roussel B, Langin D, Viguerie N, Diogenes C
Citation(s) 20739421
Submission date Dec 15, 2009
Last update date Feb 22, 2018
Contact name Nathalie Viguerie
Organization name INSERM
Department I2MC U1297
Lab MetaDiab
Street address Avenue Poulhes
City Toulouse
ZIP/Postal code 31400
Country France
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (88)
GSM486132 Successful subject under LGI/LP diet at CID2 subject 1
GSM486133 Successful subject under LGI/LP diet at CID2 subject 2
GSM486134 Successful subject under LGI/LP diet at CID2 subject 3
Relations
BioProject PRJNA122409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19494_RAW.tar 997.8 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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