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| Status |
Public on Dec 01, 2022 |
| Title |
Single-cell RNA-seq of the tendon enthesis cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our studies used scRNA-seq analysis to get a full map of transcriptional profiles of enthesis cells and also a seprate Gli1-lineage enthesis stem cells. By harvesting enthesis cells from different development stages for scRNA-seq analysis, we revealed enthesis cell heterogeneity and identified six cell sub-populations using scRNA-seq. We infered cell differentiation trajectories for enthesis stem cells differentiating into mineralizing chondrocytes. A gene regulatory network analysis combined fluorescent in situ hybridization were then used to identify a number of transcription factors coordinating tenogenesis, chondrogenesis, and osteogenesis to form an enthesis with spatially graded mineralization.To further define the enthesis stem cell population, enthesis Gli1-lineage cells were isolated and their transcriptomes were characterized at single cell resolution. These specific Gli1-responsive cells had a linear trajectory and a capacity of chondrogenesis and osteogenesis. The full characterization of transcriptional landscape of tendon enthesis stem cells demonstrates a promising therapeutic strategies using this cell source for enthesis regeneration.
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| Overall design |
mRNA profiles of of tendon enthesis at P11, P18, and P56 old wild type (WT); mRNA profiles of Gli1-lineage cells at P7, P11, P14, P18, P21, P28, and P42
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| Contributor(s) |
Fang F, Thomopoulos S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 29, 2021 |
| Last update date |
Dec 03, 2022 |
| Contact name |
Fei Fang |
| E-mail(s) |
ff2412@cumc.columbia.edu
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| Phone |
3142506188
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| Organization name |
Columbia University
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| Department |
Orthopedic Surgery
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| Lab |
Carroll Laboratories for Orthopedic Surgery
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| Street address |
650 W 168th Street, Blacking Building 1408
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA758694 |
| SRA |
SRP334617 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE182997_RAW.tar |
123.3 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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