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Series GSE107960 Query DataSets for GSE107960
Status Public on Mar 12, 2018
Title Pancreatic tumor microenvironment confers highly malignant properties on pancreatic cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Tumor microenvironment plays a pivotal role in cancer progression; however, little is known regarding how differences in the microenvironment affect characteristics of cancer cells. Here, we investigated the effects of tumor microenvironment on cancer cells by using mouse tumor models. After 3 cycles of inoculation and extraction of human pancreatic cancer cells, including SUIT-2 and Panc-1 cells, from tumors, distinct cancer cell lines were established; 3P cells from the pancreas obtained using the orthotopic tumor model, and 3sc cells from subcutaneous tissue obtained using the subcutaneous tumor model. On cell re-inoculation of these cells, the 3sc cells and, more prominently, the 3P cells, exhibited higher tumorigenic activity than the parental cells. The 3P cells specifically exhibited low E-cadherin expression and high invasiveness, suggesting that they were endowed with the highest malignant characteristics. RNA-sequence analysis demonstrated that distinct signaling pathways were activated in each cell line and that the 3P cells acquired a cancer stem cell-like phenotype. Among cancer stem cell-related genes, those specifically expressed in the 3P cells, including NES, may be potential new targets for cancer therapy. The mechanisms underlying the development of highly malignant cancer cell lines were investigated. Individual clones within the parental cells varied in tumor-forming ability, indicating the presence of cellular heterogeneity. Moreover, the gene expression profile of each clone changed after orthotopic inoculation. The present study thus suggests that both selection and education processes are involved in the development of highly malignant cancer cells.
Overall design Expression of mRNA in the highly malignant sublines of SUIT-2 and Panc-1 cells xenografted into mice.
Contributor(s) Takahashi K, Ehata S, Koinuma D, Morishita Y, Soda M, Mano H, Miyazono K
Citation(s) 29511349
Submission date Dec 12, 2017
Last update date Mar 01, 2019
Contact name Daizo Koinuma
Organization name University of Tokyo
Department Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (12)
GSM2884275 RNA-seq-parental-SUIT-2
GSM2884276 RNA-seq-SUIT-2-3P1
GSM2884277 RNA-seq-SUIT-2-3P2
BioProject PRJNA422050
SRA SRP126547

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Supplementary file Size Download File type/resource
GSE107960_genes_PANC-1.fpkm_tracking.gz 1.5 Mb (ftp)(http) FPKM_TRACKING
GSE107960_genes_SUIT-2.fpkm_tracking.gz 1.7 Mb (ftp)(http) FPKM_TRACKING
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Processed data are available on Series record
Raw data are available in SRA

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