NIA2 nitrate reductase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Gene symbol: NIA2
Gene description: nitrate reductase 2
Primary source: Araport:AT1G37130
Locus tag: AT1G37130
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS NITRATE REDUCTASE 2; ATNR2; B29; CHL3; CHLORATE RESISTANT 3; F28L22.2; F28L22_2; NIA2-1; NITRATE REDUCTASE; nitrate reductase 2; NITRATE REDUCTASE 2; NR; NR2
Summary: Identified as a mutant resistant to chlorate. Encodes nitrate reductase structural gene. Involved in nitrate assimilation. Has nitrate reductase activity. Up-regulated by the fungus P. indica. Binds transcription factor At2g35940.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

Location:: chromosome: 1

Exon count:: 3

Sequence:: Chromosome: 1; NC_003070.9 (14158452..14161938)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Discovery of a second-site nia2 mutation in the background of multiple Arabidopsis PIF-related mutants containing the pif3-3 allele.
Title: Discovery of a second-site nia2 mutation in the background of multiple Arabidopsis PIF-related mutants containing the pif3-3 allele.
Pseudomonas aeruginosa LasI-dependent plant growth promotion requires the host nitrate transceptor AtNRT1.1/CHL1 and the nitrate reductases NIA1 and NIA2.
Title: Pseudomonas aeruginosa LasI-dependent plant growth promotion requires the host nitrate transceptor AtNRT1.1/CHL1 and the nitrate reductases NIA1 and NIA2.
Study show that transcripts of both genes are present throughout the life cycle of Arabidopsis thaliana plants with NIA1 being predominantly active in leaves and NIA2 in meristematic tissues.
Title: Tissue-specific NIA1 and NIA2 expression in Arabidopsis thaliana.
Findings showed that each NR, NIA 1 and NIA2, gene differentially contributes to reaching the optimum level of NO during the gravitropic response. In seedlings, NIA2 isoform is responsible for 90% of the total NR activity
Title: Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots.
Cell wall damage enhances cytokinin degradation rates through a NIA1/2-mediated process, leading to attenuation of cell cycle gene expression.
Title: Cell wall integrity modulates Arabidopsis thaliana cell cycle gene expression in a cytokinin- and nitrate reductase-dependent manner.
mutating NIA1 and NIA2 impaired nearly all the key components of guard cell abscissic acid signaling pathway in Arabidopsis.
Title: Loss of nitrate reductases NIA1 and NIA2 impairs stomatal closure by altering genes of core ABA signaling components in Arabidopsis.
gene expression and abscissic acid signalling transduction were compared in wild-type Columbia-0 (Col-0) and the nitrate reductase mutant nia1nia2.
Title: Nitrate reductase mutation alters potassium nutrition as well as nitric oxide-mediated control of guard cell ion channels in Arabidopsis.
in response to carbon dioxide elevation, either the NR activity induction in low-nitrate plants or the NR activity inhibition in high-nitrate plants is regulated by NOS-generated nitric oxide.
Title: Elevated CO2-induced production of nitric oxide (NO) by NO synthase differentially affects nitrate reductase activity in Arabidopsis plants under different nitrate supplies.
nitrate reductase (NR) is the first enzyme that harbors two independent 14-3-3 binding sites with different affinities, which both need to be occupied by 14-3-3omega to confer full inhibition of NR activity under physiological conditions.
Title: Dual binding of 14-3-3 protein regulates Arabidopsis nitrate reductase activity.
Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH
Title: A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

NM_103364.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables FAD binding	ISS Inferred from Sequence or Structural Similarity more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables molybdenum ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables molybdopterin cofactor binding	IEA Inferred from Electronic Annotation more info
enables nitrate reductase (NADH) activity	IDA Inferred from Direct Assay more info	PubMed
enables nitrate reductase (NADPH) activity	IEA Inferred from Electronic Annotation more info
enables nitrate reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitrate reductase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within nitrate assimilation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to light stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to symbiotic fungus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	ISM Inferred from Sequence Model more info
located_in nucleus	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: nitrate reductase 2

NP_174901.1

nitrate reductase 2 (NIA2); FUNCTIONS IN: nitrate reductase activity, nitrate reductase (NADH) activity; INVOLVED IN: nitric oxide biosynthetic process, nitrate assimilation, response to light stimulus, response to symbiotic fungus; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Nitrate reductase NADH dependent (InterPro:IPR012137), Cytochrome b5, heme-binding site (InterPro:IPR018506), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Immunoglobulin E-set (InterPro:IPR014756), Cytochrome b5 (InterPro:IPR001199), NADH:cytochrome b5 reductase (CBR) (InterPro:IPR001834), Oxidoreductase, molybdopterin-binding domain (InterPro:IPR000572), Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Oxidoreductase, molybdopterin binding site (InterPro:IPR022407), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Eukaryotic molybdopterin oxidoreductase (InterPro:IPR008335), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Moybdenum cofactor oxidoreductase, dimerisation (InterPro:IPR005066); BEST Arabidopsis thaliana protein match is: nitrate reductase 1 (TAIR:AT1G77760.1); Has 14440 Blast hits to 14066 proteins in 2196 species: Archae - 142; Bacteria - 6584; Metazoa - 1752; Fungi - 2225; Plants - 1476; Viruses - 3; Other Eukaryotes - 2258 (source: NCBI BLink).