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    DET2 3-oxo-5-alpha-steroid 4-dehydrogenase family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 818383, updated on 15-Feb-2024

    Summary

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    Gene symbol
    DET2
    Gene description
    3-oxo-5-alpha-steroid 4-dehydrogenase family protein
    Primary source
    Araport:AT2G38050
    Locus tag
    AT2G38050
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATDET2; DE-ETIOLATED 2; DWARF 6; DWF6; STEROID REDUCTASE DET2; T8P21.4; T8P21_4
    Summary
    Similar to mammalian steroid-5-alpha-reductase. Involved in the brassinolide biosynthetic pathway.
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    Genomic context

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    Location:
    chromosome: 2
    Exon count:
    2
    Sequence:
    Chromosome: 2; NC_003071.7 (15921136..15922321, complement)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Cysteine proteinases superfamily protein Neighboring gene tRNA-Glu Neighboring gene acetyl Co-enzyme a carboxylase carboxyltransferase alpha subunit Neighboring gene phosphate transporter 4;2 Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Ultrastructural characterization of exine development of the transient defective exine 1 mutant suggests the existence of a factor involved in constructing reticulate exine architecture from sporopollenin aggregates. Ariizumi T, et al. Plant Cell Physiol, 2008 Jan. PMID 18045813
    2. Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis. Noguchi T, et al. Plant Physiol, 1999 Jul. PMID 10398719, Free PMC Article
    3. Conservation of function between mammalian and plant steroid 5alpha-reductases. Li J, et al. Proc Natl Acad Sci U S A, 1997 Apr 15. PMID 9108014, Free PMC Article
    4. A role for brassinosteroids in light-dependent development of Arabidopsis. Li J, et al. Science, 1996 Apr 19. PMID 8602526
    5. A chemical genetics approach reveals a role of brassinolide and cellulose synthase in hypocotyl elongation of etiolated Arabidopsis seedlings. Chen IJ, et al. Plant Sci, 2013 Aug. PMID 23759102

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. together with eto1, cesa6(ret8) and det2(ret41) exhibited an enhanced phenotype in the hypocotyls and apical hooks of etiolated seedlings.
      Title: A chemical genetics approach reveals a role of brassinolide and cellulose synthase in hypocotyl elongation of etiolated Arabidopsis seedlings.
    2. Down-regulation of the DET2:DWF4:SMT2 genes in transgenic Arabidopsis leads to a selective modification of the campesterol and brassinosteroid profiles.
      Title: Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana.
    3. The isolation and characterization of transient defective exine 1 (tde1), an alleles of DET2, is reported.
      Title: Ultrastructural characterization of exine development of the transient defective exine 1 mutant suggests the existence of a factor involved in constructing reticulate exine architecture from sporopollenin aggregates.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_129361.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables 3-oxo-5-alpha-steroid 4-dehydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within brassinosteroid biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in brassinosteroid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within brassinosteroid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within brassinosteroid homeostasis IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to light stimulus TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cellular_component ND
    No biological Data available
    more info
    		  

    General protein information

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    Preferred Names
    3-oxo-5-alpha-steroid 4-dehydrogenase family protein
    NP_181340.1
    • DE-ETIOLATED 2 (DET2); FUNCTIONS IN: sterol 5-alpha reductase activity; INVOLVED IN: response to light stimulus, brassinosteroid homeostasis, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system, integral to membrane, membrane, cytoplasm; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal (InterPro:IPR001104), 3-oxo-5-alpha-steroid 4-dehydrogenase (InterPro:IPR016636); BEST Arabidopsis thaliana protein match is: 3-oxo-5-alpha-steroid 4-dehydrogenase family protein (TAIR:AT5G16010.1); Has 1601 Blast hits to 1599 proteins in 320 species: Archae - 0; Bacteria - 193; Metazoa - 442; Fungi - 195; Plants - 239; Viruses - 0; Other Eukaryotes - 532 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      15921136..15922321 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_129361.5 → NP_181340.1  3-oxo-5-alpha-steroid 4-dehydrogenase family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_181340.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q38944, Q9SH83
      UniProtKB/TrEMBL
      A0A178VNR4, A0A5S9X506
      Conserved Domains (1) summary
      PLN02392
      Location:2 → 262
      PLN02392; probable steroid reductase DET2
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q38944.2

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