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    DNAJB1 DnaJ heat shock protein family (Hsp40) member B1 [ Homo sapiens (human) ]

    Gene ID: 3337, updated on 9-Jun-2024

    Summary

    Official Symbol
    DNAJB1provided by HGNC
    Official Full Name
    DnaJ heat shock protein family (Hsp40) member B1provided by HGNC
    Primary source
    HGNC:HGNC:5270
    See related
    Ensembl:ENSG00000132002 MIM:604572; AllianceGenome:HGNC:5270
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
    Summary
    This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
    Expression
    Ubiquitous expression in esophagus (RPKM 87.8), adrenal (RPKM 84.6) and 24 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See DNAJB1 in Genome Data Viewer
    Location:
    19p13.12
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (14514769..14560391, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (14641477..14655999, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (14625581..14640083, complement)

    Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14586151-14586819 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:14587236-14587718 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14589159-14589674 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14590760-14591354 Neighboring gene prostaglandin E receptor 1 Neighboring gene GIPC PDZ domain containing family member 1 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14606254-14607180 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10255 Neighboring gene small nuclear ribonucleoprotein polypeptide G pseudogene 15 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14608461-14608960 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10256 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10257 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr19:14630503-14631232 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:14640150-14640734 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14172 Neighboring gene microRNA 639 Neighboring gene trans-2,3-enoyl-CoA reductase Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14662493-14662992 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14666446-14667327 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14667328-14668208 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14673655-14674462 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10260 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14676079-14676885 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14173 Neighboring gene NADH:ubiquinone oxidoreductase subunit B7 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14174 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:14693526-14693669 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14175 Neighboring gene RNA, 7SL, cytoplasmic 337, pseudogene Neighboring gene C-type lectin domain containing 17A

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Nef nef HIV-1 Subtype C Nef upregulates the expression of Hsp40 in HIV-1 infected CEM cells and enhances the increased translocation of Hsp40 into the nucleus PubMed
    nef HIV-1 subtype C Nef interacts with Hsp40 in immunoprecipitation and immunofluorescence staining experiments PubMed
    nef HIV-1 Nef interacts with Hsp40, leading to increased Hsp40 translocation to the nucleus of infected cells and facilitation of viral gene expression by complex formation of Hsp40 with the cdk9-associated transcription complex PubMed
    Tat tat HIV-1 Tat specifically associates with DNAJB1 promoter to upregulate DNAJB1 expression in T cells PubMed
    capsid gag HSP40B1 (DNAJB1) inhibits HIV-1 production as measured by the level of viral p24 capsid in the culture supernatant PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATPase activator activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables ATPase activator activity TAS
    Traceable Author Statement
    more info
     
    enables ATPase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables Hsp70 protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables Hsp70 protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables cadherin binding HDA PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein folding chaperone IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein-folding chaperone binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables transcription corepressor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables transcription corepressor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables transcription regulator inhibitor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables unfolded protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables unfolded protein binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in dendritic spine IEA
    Inferred from Electronic Annotation
    more info
     
    located_in extracellular exosome HDA PubMed 
    located_in glutamatergic synapse IEA
    Inferred from Electronic Annotation
    more info
     
    located_in neuronal cell body IEA
    Inferred from Electronic Annotation
    more info
     
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
     
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    located_in nucleus HDA PubMed 
    located_in postsynaptic density IEA
    Inferred from Electronic Annotation
    more info
     
    located_in sperm head IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    dnaJ homolog subfamily B member 1
    Names
    DnaJ (Hsp40) homolog, subfamily B, member 1
    dnaJ protein homolog 1
    heat shock 40 kDa protein 1
    human DnaJ protein 1
    radial spoke 16 homolog B

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001300914.2NP_001287843.1  dnaJ homolog subfamily B member 1 isoform 2

      See identical proteins and their annotated locations for NP_001287843.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).
      Source sequence(s)
      AC012318, BC013157, D49547, DA377896
      Consensus CDS
      CCDS74295.1
      UniProtKB/TrEMBL
      A0A7I2V3K7
      Related
      ENSP00000471073.2, ENST00000598235.2
      Conserved Domains (1) summary
      cd10747
      Location:62226
      DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
    2. NM_001313964.2NP_001300893.1  dnaJ homolog subfamily B member 1 isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) contains alternate 5' terminal exons, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).
      Source sequence(s)
      AC009004, AC012318
      Consensus CDS
      CCDS74295.1
      UniProtKB/TrEMBL
      A0A7I2V3K7
      Related
      ENSP00000444212.1, ENST00000396969.8
      Conserved Domains (1) summary
      cd10747
      Location:62226
      DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
    3. NM_006145.3NP_006136.1  dnaJ homolog subfamily B member 1 isoform 1

      See identical proteins and their annotated locations for NP_006136.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
      Source sequence(s)
      BC013157, D49547, DB454482
      Consensus CDS
      CCDS12312.1
      UniProtKB/Swiss-Prot
      B4DX52, P25685
      UniProtKB/TrEMBL
      Q6FHS4, Q6FI51
      Related
      ENSP00000254322.1, ENST00000254322.3
      Conserved Domains (1) summary
      COG0484
      Location:1340
      DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

      Range
      14514769..14560391 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047438745.1XP_047294701.1  dnaJ homolog subfamily B member 1 isoform X1

      UniProtKB/TrEMBL
      A0A7I2V3K7
      Related
      ENSP00000504527.1, ENST00000679223.1
    2. XM_006722733.3XP_006722796.1  dnaJ homolog subfamily B member 1 isoform X1

      See identical proteins and their annotated locations for XP_006722796.1

      UniProtKB/TrEMBL
      A0A7I2V3K7
      Related
      ENSP00000471798.2, ENST00000601533.6
      Conserved Domains (1) summary
      cd10747
      Location:62226
      DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
    3. XM_011527956.4XP_011526258.1  dnaJ homolog subfamily B member 1 isoform X1

      See identical proteins and their annotated locations for XP_011526258.1

      UniProtKB/TrEMBL
      A0A7I2V3K7
      Related
      ENSP00000472886.2, ENST00000598692.2
      Conserved Domains (1) summary
      cd10747
      Location:62226
      DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
    4. XM_047438746.1XP_047294702.1  dnaJ homolog subfamily B member 1 isoform X1

      UniProtKB/TrEMBL
      A0A7I2V3K7
      Related
      ENSP00000470063.2, ENST00000596853.6

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060943.1 Alternate T2T-CHM13v2.0

      Range
      14641477..14655999 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054320814.1XP_054176789.1  dnaJ homolog subfamily B member 1 isoform X1

      UniProtKB/TrEMBL
      A0A7I2V3K7