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    Pkm pyruvate kinase, muscle [ Mus musculus (house mouse) ]

    Gene ID: 18746, updated on 27-Sep-2022

    Summary

    Official Symbol
    Pkmprovided by MGI
    Official Full Name
    pyruvate kinase, muscleprovided by MGI
    Primary source
    MGI:MGI:97591
    See related
    Ensembl:ENSMUSG00000032294 AllianceGenome:MGI:97591
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Pk3; Pk-2; Pk-3; Pkm2
    Summary
    Enables mRNA binding activity and pyruvate kinase activity. Involved in positive regulation of cytoplasmic translation. Acts upstream of or within glycolytic process. Located in cilium; photoreceptor inner segment; and rough endoplasmic reticulum. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Orthologous to human PKM (pyruvate kinase M1/2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in duodenum adult (RPKM 494.4), mammary gland adult (RPKM 485.2) and 27 other tissues See more
    Orthologs
    NEW
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    Genomic context

    See Pkm in Genome Data Viewer
    Location:
    9 B; 9 32.03 cM
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    109 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (59563859..59586655)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (59656576..59679372)

    Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 51709 Neighboring gene CUGBP, Elav-like family member 6 Neighboring gene poly (ADP-ribose) polymerase family, member 6 Neighboring gene predicted gene, 51708 Neighboring gene uncharacterized LOC115487221 Neighboring gene GRAM domain containing 2 Neighboring gene SUMO peptidase family member, NEDD8 specific

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    • Homologs of the Pkm gene: The Pkm gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
    • Orthologs from Annotation Pipeline: 300 organisms have orthologs with human gene Pkm
    • Orthologs

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ADP binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables mRNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables non-membrane spanning protein tyrosine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables potassium ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein dimerization activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein tyrosine kinase activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables pyruvate kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables pyruvate kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables pyruvate kinase activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables thyroid hormone binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables transcription coactivator activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    involved_in ATP biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in animal organ regeneration ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in canonical glycolysis IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in cellular response to insulin stimulus IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in glucose metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in glycolytic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    acts_upstream_of_or_within glycolytic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in glycolytic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in liver development ISO
    Inferred from Sequence Orthology
    more info
     
    acts_upstream_of_or_within metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of cytoplasmic translation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of sprouting angiogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in programmed cell death ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in protein homotetramerization ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in protein tetramerization ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in pyruvate biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in pyruvate metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    acts_upstream_of_or_within regulation of translation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in skeletal muscle tissue regeneration ISO
    Inferred from Sequence Orthology
    more info
     
    Component Evidence Code Pubs
    located_in cilium IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in collagen-containing extracellular matrix HDA PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
     
    located_in mitochondrion HDA PubMed 
    located_in myelin sheath HDA PubMed 
    is_active_in nucleus ISO
    Inferred from Sequence Orthology
    more info
     
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
     
    part_of pyruvate kinase complex ISO
    Inferred from Sequence Orthology
    more info
     
    located_in rough endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    pyruvate kinase PKM
    Names
    pyruvate kinase 3
    pyruvate kinase isozymes M1/M2
    pyruvate kinase muscle isozyme
    threonine-protein kinase PKM2
    tyrosine-protein kinase PKM2
    NP_001240812.1
    NP_001365795.1
    NP_001365796.1
    NP_001365797.1
    NP_001365798.1
    NP_001365799.1
    NP_001365800.1
    NP_001365801.1
    NP_035229.2
    XP_006510918.1
    XP_006510919.1
    XP_011240976.2
    XP_036010583.1
    XP_036010584.1
    XP_036010585.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001253883.2NP_001240812.1  pyruvate kinase PKM isoform M1

      See identical proteins and their annotated locations for NP_001240812.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes isoform M1. Isoforms M1 and M2 are identical in length but have different sequences. Variants 1, 3, and 4 all encode the same isoform (M1).
      Source sequence(s)
      AC160637
      Consensus CDS
      CCDS57681.1
      UniProtKB/Swiss-Prot
      P52480
      UniProtKB/TrEMBL
      A0A6P5QA21, A0A8C6IEX1
      Related
      ENSMUSP00000128770.3, ENSMUST00000163694.4
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    2. NM_001378866.1NP_001365795.1  pyruvate kinase PKM isoform M1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3), as well as variants 1 and 4, encodes isoform M1.
      Source sequence(s)
      AC160637
      UniProtKB/TrEMBL
      A0A6P5QA21, A0A8C6IEX1
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    3. NM_001378867.1NP_001365796.1  pyruvate kinase PKM isoform M1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4), as well as variants 1 and 3, encodes isoform M1.
      Source sequence(s)
      AC160637
      UniProtKB/TrEMBL
      A0A6P5QA21, A0A8C6IEX1
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    4. NM_001378868.1NP_001365797.1  pyruvate kinase PKM isoform M2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5), as well as variants 2, 6, and 7, encodes isoform M2.
      Source sequence(s)
      AC160637
      UniProtKB/Swiss-Prot
      Q9CWB1
      UniProtKB/TrEMBL
      A0A6P5QC04, A0A8C6IE47
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    5. NM_001378869.1NP_001365798.1  pyruvate kinase PKM isoform M2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6), as well as variants 2, 5, and 7, encodes isoform M2.
      Source sequence(s)
      AC160637
      UniProtKB/Swiss-Prot
      Q9CWB1
      UniProtKB/TrEMBL
      A0A6P5QC04, A0A8C6IE47
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    6. NM_001378870.1NP_001365799.1  pyruvate kinase PKM isoform M2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (7), as well as variants 2, 5, and 6, encodes isoform M2.
      Source sequence(s)
      AC160637
      UniProtKB/Swiss-Prot
      Q9CWB1
      UniProtKB/TrEMBL
      A0A6P5QC04, A0A8C6IE47
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    7. NM_001378871.1NP_001365800.1  pyruvate kinase PKM isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (8) represents the longest transcript and encodes the longest isoform (3).
      Source sequence(s)
      AC160637
      Conserved Domains (1) summary
      cd00288
      Location:102590
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    8. NM_001378872.1NP_001365801.1  pyruvate kinase PKM isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC160637
      Conserved Domains (1) summary
      cd00288
      Location:63551
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    9. NM_001405491.1NP_001392420.1  pyruvate kinase PKM isoform M1

      Status: VALIDATED

      Source sequence(s)
      AC160637
      UniProtKB/TrEMBL
      A0A6P5QA21, A0A8C6IEX1
    10. NM_011099.4NP_035229.2  pyruvate kinase PKM isoform M2

      See identical proteins and their annotated locations for NP_035229.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an in-frame exon and contains an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (M2) is identical in length to isoform M1, but has a different sequence. Variants 2 and 5-7 all encode the same isoform (M2).
      Source sequence(s)
      AC160637
      Consensus CDS
      CCDS40659.1
      UniProtKB/Swiss-Prot
      P52480, Q9CWB1
      UniProtKB/TrEMBL
      A0A6P5QC04, A0A8C6IE47
      Related
      ENSMUSP00000034834.10, ENSMUST00000034834.16
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...

    RNA

    1. NR_166145.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC160637
    2. NR_166146.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC160637

    RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000075.7 Reference GRCm39 C57BL/6J

      Range
      59563859..59586655
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011242674.2XP_011240976.2  pyruvate kinase PKM isoform X1

      Conserved Domains (1) summary
      cd00288
      Location:115603
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    2. XM_036154691.1XP_036010584.1  pyruvate kinase PKM isoform X3

      Conserved Domains (1) summary
      cd00288
      Location:102590
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    3. XM_006510855.1XP_006510918.1  pyruvate kinase PKM isoform X4

      See identical proteins and their annotated locations for XP_006510918.1

      UniProtKB/Swiss-Prot
      P52480, Q9CWB1
      UniProtKB/TrEMBL
      A0A6P5QC04, A0A8C6IE47
      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    4. XM_036154692.1XP_036010585.1  pyruvate kinase PKM isoform X5

      Conserved Domains (1) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    5. XM_006510856.3XP_006510919.1  pyruvate kinase PKM isoform X6

      Conserved Domains (1) summary
      cd00288
      Location:68515
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
    6. XM_036154690.1XP_036010583.1  pyruvate kinase PKM isoform X2

      Conserved Domains (1) summary
      cd00288
      Location:112600
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...