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EIF5B eukaryotic translation initiation factor 5B [ Homo sapiens (human) ]

Gene ID: 9669, updated on 9-Sep-2018

Summary

Official Symbol
EIF5Bprovided by HGNC
Official Full Name
eukaryotic translation initiation factor 5Bprovided by HGNC
Primary source
HGNC:HGNC:30793
See related
Ensembl:ENSG00000158417 MIM:606086; Vega:OTTHUMG00000153242
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
IF2
Summary
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in heart (RPKM 26.2), testis (RPKM 23.3) and 25 other tissues See more
Orthologs

Genomic context

See EIF5B in Genome Data Viewer
Location:
2q11.2
Exon count:
24
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 2 NC_000002.12 (99337371..99400265)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (99953821..100016728)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107985923 Neighboring gene thioredoxin domain containing 9 Neighboring gene lysozyme g1 Neighboring gene REV1, DNA directed polymerase Neighboring gene AF4/FMR2 family member 3 Neighboring gene uncharacterized LOC105373504

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Phenotypes

NHGRI GWAS Catalog

Description
Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.
NHGRI GWA Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag HIV-1 Gag interacts with EIF5B as demonstrated by proximity dependent biotinylation proteomics PubMed
matrix gag HIV-1 Matrix inhibits hIF2 mediated translation in a reticulocyte lysate assay PubMed
gag HIV-1 Matrix, as well as the full length HIV-1 Gag protein bind to human translation initiation factor two (hIF2) and these proteins can be immunoprecipitated as a protein complex PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Cap-dependent Translation Initiation, organism-specific biosystem (from REACTOME)
    Cap-dependent Translation Initiation, organism-specific biosystemTranslation initiation is a complex process in which the Met-tRNAi initiator, 40S, and 60S ribosomal subunits are assembled by eukaryotic initiation factors (eIFs) into an 80S ribosome at the start c...
  • Eukaryotic Translation Initiation, organism-specific biosystem (from REACTOME)
    Eukaryotic Translation Initiation, organism-specific biosystemInitiation of translation in the majority of eukaryotic cellular mRNAs depends on the 5'-cap (m7GpppN) and involves ribosomal scanning of the 5' untranslated region (5'-UTR) for an initiating AUG sta...
  • GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem (from REACTOME)
    GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystemHydrolysis of eIF2-GTP occurs after the Met-tRNAi has recognized the AUG. This reaction is catalyzed by eIF5 (or eIF5B) and is thought to cause dissociation of all other initiation factors and allow ...
  • Gene Expression, organism-specific biosystem (from REACTOME)
    Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
  • Metabolism of proteins, organism-specific biosystem (from REACTOME)
    Metabolism of proteins, organism-specific biosystemProtein metabolism comprises the pathways of translation, post-translational modification and protein folding.
  • RNA transport, organism-specific biosystem (from KEGG)
    RNA transport, organism-specific biosystemRNA transport from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes (NPCs) ...
  • RNA transport, conserved biosystem (from KEGG)
    RNA transport, conserved biosystemRNA transport from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes (NPCs) ...
  • Translation, organism-specific biosystem (from REACTOME)
    Translation, organism-specific biosystemProtein synthesis is accomplished through the process of translation of an mRNA sequence into a polypeptide chain. This process can be divided into three distinct stages: initiation, elongation and ...
  • Translation Factors, organism-specific biosystem (from WikiPathways)
    Translation Factors, organism-specific biosystemProtein synthesis is the ultimate step of gene expression and a key control point for regulation. In particular, it enables cells to rapidly manipulate protein production without new mRNA synthesis, ...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ10524, KIAA0741, DKFZp434I036

Gene Ontology Provided by GOA

Function Evidence Code Pubs
GTP binding IEA
Inferred from Electronic Annotation
more info
 
GTPase activity IEA
Inferred from Electronic Annotation
more info
 
RNA binding HDA PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
translation initiation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
translation initiation factor activity IDA
Inferred from Direct Assay
more info
PubMed 
translation initiation factor activity NAS
Non-traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
regulation of translational initiation IDA
Inferred from Direct Assay
more info
PubMed 
regulation of translational initiation NAS
Non-traceable Author Statement
more info
PubMed 
translational initiation IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
NOT nucleus IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
eukaryotic translation initiation factor 5B
Names
eIF-5B
translation initiation factor IF-2
translation initiation factor IF2
NP_056988.3

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_015904.3NP_056988.3  eukaryotic translation initiation factor 5B

    See identical proteins and their annotated locations for NP_056988.3

    Status: REVIEWED

    Source sequence(s)
    AB018284, AC018690, BC032639, BX485839
    Consensus CDS
    CCDS42721.1
    UniProtKB/Swiss-Prot
    O60841
    UniProtKB/TrEMBL
    Q8N5A0
    Related
    ENSP00000289371.5, OTTHUMP00000203504, ENST00000289371.10, OTTHUMT00000330364
    Conserved Domains (6) summary
    cd01887
    Location:632845
    IF2_eIF5B; Initiation Factor 2 (IF2)/ eukaryotic Initiation Factor 5B (eIF5B) family
    PRK04004
    Location:6271202
    PRK04004; translation initiation factor IF-2; Validated
    pfam08070
    Location:110171
    DTHCT; DTHCT (NUC029) region
    pfam11987
    Location:9661067
    IF-2; Translation-initiation factor 2
    cd03703
    Location:855965
    aeIF5B_II; Domain II of archaeal and eukaryotic Initiation Factor 5
    cd16266
    Location:10921186
    IF2_aeIF5B_IV; Domain IV of prokaryotic Initiation Factor 2 and archaeal and eukaryotic Initiation Factor 5

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p12 Primary Assembly

    Range
    99337371..99400265
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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