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MED14 mediator complex subunit 14 [ Homo sapiens (human) ]

Gene ID: 9282, updated on 10-Oct-2019

Summary

Official Symbol
MED14provided by HGNC
Official Full Name
mediator complex subunit 14provided by HGNC
Primary source
HGNC:HGNC:2370
See related
Ensembl:ENSG00000180182 MIM:300182
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in thyroid (RPKM 8.7), duodenum (RPKM 8.0) and 25 other tissues See more
Orthologs

Genomic context

See MED14 in Genome Data Viewer
Location:
Xp11.4
Exon count:
34
Annotation release Status Assembly Chr Location
109.20190905 current GRCh38.p13 (GCF_000001405.39) X NC_000023.11 (40648305..40736122, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (40508795..40595374, complement)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene chromosome X open reading frame 38 Neighboring gene mitochondrial pyruvate carrier 1 like Neighboring gene MED14 opposite strand Neighboring gene claudin 7 pseudogene Neighboring gene divergent-paired related homeobox pseudogene 6

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 14 (MED14) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
Knockdown of mediator complex subunit 14 (MED14) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat SiRNA knockdown of MED14 shows a significant inhibition in HIV-1 Tat-mediated transcription levels as compared to control PubMed
tat The interaction of HIV-1 Tat with MED21 hypothetically induces the binding of Tat to MED14 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Developmental Biology, organism-specific biosystem (from REACTOME)
    Developmental Biology, organism-specific biosystemAs a first step towards capturing the array of processes by which a fertilized egg gives rise to the diverse tissues of the body, examples of three kinds of processes have been annotated. These are a...
  • Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem (from REACTOME)
    Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystemThe reactions involved in the metabolism of fatty acids and of the triacylglycerols and ketone bodies derived from them form a closely interrelated, coordinately regulated module that plays a central...
  • Gene Expression, organism-specific biosystem (from REACTOME)
    Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
  • Generic Transcription Pathway, organism-specific biosystem (from REACTOME)
    Generic Transcription Pathway, organism-specific biosystemOVERVIEW OF TRANSCRIPTION REGULATION: Detailed studies of gene transcription regulation in a wide variety of eukaryotic systems has revealed the general principles and mechanisms by which cell- or t...
  • Metabolism, organism-specific biosystem (from REACTOME)
    Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
  • Metabolism of lipids and lipoproteins, organism-specific biosystem (from REACTOME)
    Metabolism of lipids and lipoproteins, organism-specific biosystemLipids are hydrophobic but otherwise chemically diverse molecules that play a wide variety of roles in human biology. They include ketone bodies, fatty acids, triacylglycerols, phospholipids and sphi...
  • PPARA activates gene expression, organism-specific biosystem (from REACTOME)
    PPARA activates gene expression, organism-specific biosystemThe set of genes regulated by PPAR-alpha is not fully known in humans, however many examples have been found in mice. Genes directly activated by PPAR-alpha contain peroxisome proliferator receptor e...
  • Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem (from REACTOME)
    Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystemPeroxisome proliferator-activated receptor alpha (PPAR-alpha) is the major regulator of fatty acid oxidation in the liver. PPARalpha is also the target of fibrate drugs used to treat abnormal plasma ...
  • Thyroid hormone signaling pathway, organism-specific biosystem (from KEGG)
    Thyroid hormone signaling pathway, organism-specific biosystemThe thyroid hormones (THs) are important regulators of growth, development and metabolism. The action of TH is mainly mediated by T3 (3,5,3'-triiodo-L-thyronine). Thyroid hormones, L-thyroxine (T4) a...
  • Transcriptional regulation of white adipocyte differentiation, organism-specific biosystem (from REACTOME)
    Transcriptional regulation of white adipocyte differentiation, organism-specific biosystemAdipogenesis is the process of cell differentiation by which preadipocytes become adipocytes. During this process the preadipocytes cease to proliferate, begin to accumulate lipid droplets and develo...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC104513

Gene Ontology Provided by GOA

Function Evidence Code Pubs
nuclear receptor transcription coactivator activity NAS
Non-traceable Author Statement
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
transcription coactivator activity IDA
Inferred from Direct Assay
more info
PubMed 
transcription coregulator activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
transcription coregulator activity IDA
Inferred from Direct Assay
more info
PubMed 
vitamin D receptor binding NAS
Non-traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mediator complex IDA
Inferred from Direct Assay
more info
PubMed 
membrane HDA PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
mediator of RNA polymerase II transcription subunit 14
Names
ARC150
CRSP complex subunit 2
RGR1 homolog
activator-recruited cofactor 150 kDa component
cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)
hRGR1
human homolog of yeast RGR1
thyroid hormone receptor-associated protein complex 170 kDa component
thyroid hormone receptor-associated protein complex component TRAP170
transcriptional co-activator CRSP150
vitamin D receptor-interacting protein complex component DRIP150
vitamin D3 receptor-interacting protein complex 150 kDa component

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_016325.1 RefSeqGene

    Range
    5011..92248
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_004229.4NP_004220.2  mediator of RNA polymerase II transcription subunit 14

    See identical proteins and their annotated locations for NP_004220.2

    Status: REVIEWED

    Source sequence(s)
    AC093029, AF304448, BC098377
    Consensus CDS
    CCDS14254.1
    UniProtKB/Swiss-Prot
    O60244
    Related
    ENSP00000323720.1, ENST00000324817.6
    Conserved Domains (2) summary
    pfam08638
    Location:51238
    Med14; Mediator complex subunit MED14
    cl26464
    Location:9681196
    Atrophin-1; Atrophin-1 family

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109.20190905

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p13 Primary Assembly

    Range
    40648305..40736122 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005272701.3XP_005272758.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

    See identical proteins and their annotated locations for XP_005272758.1

    Conserved Domains (2) summary
    pfam08638
    Location:2122
    Med14; Mediator complex subunit MED14
    cl26464
    Location:8521080
    Atrophin-1; Atrophin-1 family
  2. XM_011544000.3XP_011542302.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

    See identical proteins and their annotated locations for XP_011542302.1

    Conserved Domains (2) summary
    pfam08638
    Location:2122
    Med14; Mediator complex subunit MED14
    cl26464
    Location:8521080
    Atrophin-1; Atrophin-1 family
  3. XM_017029962.2XP_016885451.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

    Conserved Domains (2) summary
    pfam08638
    Location:2122
    Med14; Mediator complex subunit MED14
    cl26464
    Location:8521080
    Atrophin-1; Atrophin-1 family
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