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Usp8 ubiquitin specific peptidase 8 [ Mus musculus (house mouse) ]

Gene ID: 84092, updated on 2-Nov-2024

Summary

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Official Symbol
Usp8provided by MGI
Official Full Name
ubiquitin specific peptidase 8provided by MGI
Primary source
MGI:MGI:1934029
See related
Ensembl:ENSMUSG00000027363 AllianceGenome:MGI:1934029
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Ubpy; mKIAA0055
Summary
Enables cysteine-type deubiquitinase activity. Involved in endosome organization and protein deubiquitination. Acts upstream of or within Ras protein signal transduction. Located in cytoplasmic vesicle membrane; cytosol; and midbody. Is expressed in several structures, including brain; genitourinary system; gut; hemolymphoid system gland; and sensory organ. Human ortholog(s) of this gene implicated in ACTH-secreting pituitary adenoma. Orthologous to human USP8 (ubiquitin specific peptidase 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in testis adult (RPKM 45.2), bladder adult (RPKM 14.4) and 25 other tissues See more
Orthologs
human all
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Genomic context

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See Usp8 in Genome Data Viewer
Location:
2 F1; 2 61.76 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (126549280..126601234)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (126707201..126759314)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene GA repeat binding protein, beta 1 Neighboring gene zinc finger, BED domain containing 4 pseudogene Neighboring gene RIKEN cDNA A630026N12 gene Neighboring gene STARR-seq mESC enhancer starr_05607 Neighboring gene STARR-seq mESC enhancer starr_05610 Neighboring gene polymerase (RNA) II (DNA directed) polypeptide L pseudogene Neighboring gene ubiquitin specific peptidase 50 Neighboring gene transient receptor potential cation channel, subfamily M, member 7 Neighboring gene STARR-positive B cell enhancer ABC_E11158

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages. Joshi K, et al. Infect Immun, 2024 Feb 13. PMID 38174929, Free PMC Article
  2. The ESCRT-deubiquitinating enzyme USP8 in the cervical spinal cord of wild-type and Vps54-recessive (wobbler) mutant mice. Paiardi C, et al. Histochem Cell Biol, 2014 Jan. PMID 23615794
  3. Expression and localization of the deubiquitinating enzyme mUBPy in wobbler mouse testis during spermiogenesis. Chianese R, et al. Gen Comp Endocrinol, 2010 Apr 1. PMID 19800341
  4. Regulation of ubiquitin-specific processing protease 8 suppresses neuroinflammation. Zhu L, et al. Mol Cell Neurosci, 2015 Jan. PMID 24861766
  5. Expression of the deubiquitinating enzyme mUBPy in the mouse brain. Bruzzone F, et al. Brain Res, 2008 Feb 21. PMID 18206859

See all (62) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.
    Title: USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.
  2. Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages.
    Title: Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages.
  3. Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.
    Title: Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.
  4. Central role of ubiquitin-specific protease 8 in leptin signaling pathway in pulmonary arterial hypertension.
    Title: Central role of ubiquitin-specific protease 8 in leptin signaling pathway in pulmonary arterial hypertension.
  5. USP8 mitigates cognitive dysfunction of mice with sepsis-associated encephalopathy.
    Title: USP8 mitigates cognitive dysfunction of mice with sepsis-associated encephalopathy.
  6. Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious Osteomyelitis by Targeting RIPK2 Ubiquitination.
    Title: Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious Osteomyelitis by Targeting RIPK2 Ubiquitination.
  7. Ubiquitinspecific protease 8 ameliorates lipopolysaccharideinduced spleen injury via suppression of NFkappaB and MAPK signaling pathways.
    Title: Ubiquitin‑specific protease 8 ameliorates lipopolysaccharide‑induced spleen injury via suppression of NF‑κB and MAPK signaling pathways.
  8. USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.
    Title: USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.
  9. Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
    Title: Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
  10. Deubiquitination of EPG5 by USP8 guards the autophagic flux in embryonic stem cells (ESCs) to maintain their stemness. This work uncovers a novel crosstalk pathway between ubiquitination and autophagy through USP8-EPG5 interaction to regulate the stemness of ESCs.
    Title: USP8 maintains embryonic stem cell stemness via deubiquitination of EPG5.
Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Gene trapped (1) 
  • Targeted (2)  1 citation

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables K48-linked deubiquitinase activity ISO
Inferred from Sequence Orthology
more info
  
enables K63-linked deubiquitinase activity ISO
Inferred from Sequence Orthology
more info
  
enables SH3 domain binding IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-type deubiquitinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cysteine-type deubiquitinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-type deubiquitinase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Ras protein signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within Ras protein signal transduction IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in cellular response to nerve growth factor stimulus ISO
Inferred from Sequence Orthology
more info
  
involved_in endosome organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endosome organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within mitotic cytokinesis ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of lysosomal protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of amyloid fibril formation ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of canonical Wnt signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K48-linked deubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K63-linked deubiquitination ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within protein deubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein deubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein deubiquitination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein deubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein deubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of protein catabolic process at postsynapse, modulating synaptic transmission ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of protein localization ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of protein localization ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of protein stability ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of protein stability ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
located_in acrosomal membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
is_active_in dendritic spine IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in dendritic spine ISO
Inferred from Sequence Orthology
more info
  
located_in early endosome ISO
Inferred from Sequence Orthology
more info
  
located_in endosome membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse ISO
Inferred from Sequence Orthology
more info
  
located_in intracellular anatomical structure IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in midbody IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in midbody IDA
Inferred from Direct Assay
more info
 PubMed 
located_in midbody ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in postsynaptic density IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in postsynaptic density ISO
Inferred from Sequence Orthology
more info
  
located_in postsynaptic density ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
ubiquitin carboxyl-terminal hydrolase 8
Names
deubiquitinating enzyme 8
putative deubiquitinating enzyme
ubiquitin isopeptidase Y
ubiquitin thioesterase 8
ubiquitin thiolesterase 8
ubiquitin-specific-processing protease 8
NP_001239509.1
NP_001409160.1
NP_001409161.1
NP_001409162.1
NP_001409163.1
NP_062703.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001252580.2 → NP_001239509.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AL732330
    Consensus CDS
    CCDS57185.1
    UniProtKB/TrEMBL
    A2AI52, Q3UZ62
    Related
    ENSMUSP00000106046.3, ENSMUST00000110416.3
    Conserved Domains (4) summary
    smart00450
    Location:210 → 321
    RHOD; Rhodanese Homology Domain
    cd02674
    Location:751 → 1080
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:749 → 1079
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam08969
    Location:6 → 126
    USP8_dimer; USP8 dimerization domain

  2. NM_001422231.1 → NP_001409160.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AL732330

  3. NM_001422232.1 → NP_001409161.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AL732330

  4. NM_001422233.1 → NP_001409162.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AL732330

  5. NM_001422234.1 → NP_001409163.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AL732330

  6. NM_019729.4 → NP_062703.2  ubiquitin carboxyl-terminal hydrolase 8 isoform 2

    See identical proteins and their annotated locations for NP_062703.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AL732330
    Consensus CDS
    CCDS16687.1
    UniProtKB/Swiss-Prot
    A2AI53, Q80U87, Q80YP2, Q8R0D3, Q9EQU1, Q9WVP5
    UniProtKB/TrEMBL
    Q3UZ62
    Related
    ENSMUSP00000028841.8, ENSMUST00000028841.14
    Conserved Domains (4) summary
    smart00450
    Location:199 → 310
    RHOD; Rhodanese Homology Domain
    cd02674
    Location:740 → 1069
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:738 → 1068
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam08969
    Location:6 → 115
    USP8_dimer; USP8 dimerization domain

RNA

  1. NR_185498.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732330

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    126549280..126601234
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q80U87.2 GenPept UniProtKB/Swiss-Prot:Q80U87

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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