PNG1 peptide-N-glycanase 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PNG1
Official Full Name: peptide-N-glycanase 1
Primary source: TAIR:AT5G49570
Locus tag: AT5G49570
See related: Araport:AT5G49570
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtPNG1; K6M13.12; K6M13_12; peptide-N-glycanase 1
Summary: Encodes a protein that has peptide:N-glycanase activity in enzymatic assay in heterologous systems (although the activity was not detected in wild-type plants).
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Genomic context

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Location:: chromosome: 5

Exon count:: 17

Sequence:: Chromosome: 5; NC_003076.8 (20111859..20116269, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AtPng1 knockout mutant of Arabidopsis thaliana shows a juvenile phenotype, morpho-functional changes, altered stress response and cell wall modifications.
Title: AtPng1 knockout mutant of Arabidopsis thaliana shows a juvenile phenotype, morpho-functional changes, altered stress response and cell wall modifications.
The plant PNGase facilitates ERAD through its deglycosylation activity, while the catalytic mutant of AtPNG1 impair glycoprotein ERAD by binding to N-glycans on the ERAD substrates.
Title: A plant peptide: N-glycanase orthologue facilitates glycoprotein ER-associated degradation in yeast.
AtPng1p, the first plant transglutaminase sequenced shows undetectable sequence homology to the animal enzymes, except for the catalytic triad. It is, however, endowed with a calcium-dependent activity that allowed us to build a three-dimensional model.
Title: Plant and animal transglutaminases: do similar functions imply similar structures?
the AtPng1p gene product acts as a transglutaminase; the first reported plant protein, characterized at molecular level showing TGase activity. [AtPng1p]
Title: AtPng1p. The first plant transglutaminase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_124335.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within response to microbial phytotoxin	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to ozone	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to salicylic acid	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: peptide-N-glycanase 1

NP_199768.1

peptide-N-glycanase 1 (PNG1); CONTAINS InterPro DOMAIN/s: Transglutaminase-like (InterPro:IPR002931), DNA repair protein Rad4, transglutaminase-like domain (InterPro:IPR018325), Galactose-binding domain-like (InterPro:IPR008979); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

20111859..20116269 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_124335.3 → NP_199768.1 peptide-N-glycanase 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_199768.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q84WJ1, Q9FGY9

UniProtKB/TrEMBL

A0A654G9R0, A5PHD1

Conserved Domains (3) summary

pfam01841
Location:199 → 296

Transglut_core; Transglutaminase-like superfamily

cl10615
Location:280 → 347

sm_acid_XPC-like; small acidic domain of Xeroderma pigmentosum group C complementing protein and similar proteins

cl28922
Location:16 → 63

Ubiquitin_like_fold; Beta-grasp ubiquitin-like fold