TOC75-III translocon at the outer envelope membrane of chloroplasts 75-III [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: TOC75-III
Official Full Name: translocon at the outer envelope membrane of chloroplasts 75-III
Primary source: TAIR:AT3G46740
Locus tag: AT3G46740
See related: Araport:AT3G46740
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: MAR1; MODIFIER OF ARG1 1; translocon at the outer envelope membrane of chloroplasts 75-III
Summary: Component of the translocon outer membrane (TOC) complex. Forms the outer envelope translocation channel (beta-barrel). Plays a role in preprotein conductance. Imported into chloroplast. Expressed in young dividing photosynthetic tissues. Knockout mutants are embryo lethal with arrested development at the two-cell stage. Knockout mutants have abnormal etioplasts.
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Genomic context

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See TOC75-III in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 7

Sequence:: Chromosome: 3; NC_003074.8 (17215813..17219411, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Toc75 polypeptide transport-associated (POTRA) domains are located in the intermembrane space of the chloroplast envelope by immunogold electron microscopy.
Title: Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts.
Data indicate that OEP80-T7(atToc75-V) and atToc75-III showed distinct migration patterns on blue native-PAGE.
Title: OEP80, an essential protein paralogous to the chloroplast protein translocation channel Toc75, exists as a 70-kD protein in the Arabidopsis thaliana chloroplast outer envelope.
the POTRA domains of both Toc75-III and Toc75-V are exposed to the cytoplasm
Title: Chloroplast Omp85 proteins change orientation during evolution.
SPase I converted Toc75 precursor to its mature form in vitro.
Title: Complete maturation of the plastid protein translocation channel requires a type I signal peptidase.
Embryo development in the atToc75-III mutant, lacking the pore forming component of the translocase, was arrested extremely early at the two-cell stage.
Title: Deletion of core components of the plastid protein import machinery causes differential arrest of embryo development in Arabidopsis thaliana.
atTOC75-III of the Toc75 (translocon at the outer envelope membrane of chloroplasts, 75 kD) gene family is essential for plant viability, and is expressed at its highest level in young, rapidly expanding tissues
Title: A molecular-genetic study of the Arabidopsis Toc75 gene family.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_114541.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-transporting ATPase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within chloroplast organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chloroplast organization	ISS Inferred from Sequence or Structural Similarity more info	PubMed
acts_upstream_of_or_within embryonic morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein import into chloroplast stroma	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein targeting to chloroplast	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of TOC-TIC supercomplex I	IDA Inferred from Direct Assay more info	PubMed
part_of Toc complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast envelope	HDA more info	PubMed
located_in chloroplast outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in plastid	HDA more info	PubMed

General protein information

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Preferred Names: translocon at the outer envelope membrane of chloroplasts 75-III

NP_190258.1

translocon at the outer envelope membrane of chloroplasts 75-III (TOC75-III); FUNCTIONS IN: P-P-bond-hydrolysis-driven protein transmembrane transporter activity; INVOLVED IN: protein targeting to chloroplast, chloroplast organization, embryonic morphogenesis; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Bacterial surface antigen (D15) (InterPro:IPR000184), Chloroplast envelope protein translocase, IAP75 (InterPro:IPR005689), Surface antigen variable number (InterPro:IPR010827); BEST Arabidopsis thaliana protein match is: Outer membrane OMP85 family protein (TAIR:AT4G09080.1); Has 4030 Blast hits to 1859 proteins in 343 species: Archae - 0; Bacteria - 806; Metazoa - 1937; Fungi - 82; Plants - 633; Viruses - 22; Other Eukaryotes - 550 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

17215813..17219411 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_114541.3 → NP_190258.1 translocon at the outer envelope membrane of chloroplasts 75-III [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_190258.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9STE8

UniProtKB/TrEMBL

A0A178VEL0

Conserved Domains (2) summary

PLN03138
Location:8 → 818

PLN03138; Protein TOC75; Provisional

pfam01103
Location:486 → 815

Bac_surface_Ag; Surface antigen