Ckap2 cytoskeleton associated protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ckap2provided by MGI
Official Full Name: cytoskeleton associated protein 2provided by MGI
Primary source: MGI:MGI:1931797
See related: Ensembl:ENSMUSG00000037725 AllianceGenome:MGI:1931797
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: LB1
Summary: Acts upstream of or within negative regulation of microtubule depolymerization. Located in microtubule cytoskeleton. Is expressed in brain ventricular layer; cerebral cortex subventricular zone; and thymus primordium. Orthologous to human CKAP2 (cytoskeleton associated protein 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in CNS E11.5 (RPKM 15.6), liver E14 (RPKM 9.6) and 13 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 A2; 8 11.13 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (22658168..22675835, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (22168149..22185819, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules.
Title: The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules.
The results suggest that the phosphorylation of Cytoskeleton Associated Protein 2 (CKAP2) at T603 and possibly S608 phosphorylation sites by Cyclin Dependent Kinase 1 (CDK1) during mitosis is essential for maintaining the number of centrosomes.
Title: CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity.
we found that Lb1-null epicardial cells have a delayed nuclear morphology change in vivo, suggesting that Lb1 facilitates morphological changes associated with migration. These findings suggest that Lb1 contributes to nuclear shape maintenance and migration of epicardial cells
Title: Lamin-B1 contributes to the proper timing of epicardial cell migration and function during embryonic heart development.
These data suggest a physiological role of CKAP2 in the formation of spindle bipolarity, which is necessary for maintaining chromosomal stability.
Title: CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte.
Our present findings demonstrate that TMAP/CKAP2 is essential for proper chromosome segregation and for maintaining genomic stability.
Title: TMAP/CKAP2 is essential for proper chromosome segregation.
The cellular functions of TMAP/CKAP2 might be regulated by timely phosphorylation and dephosphorylation during the course of mitosis.
Title: Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis.
These findings suggest that CKAP2 is a new MAP with microtubule-stabilizing properties
Title: Identification of a mouse cytoskeleton-associated protein, CKAP2, with microtubule-stabilizing properties.
Data suggest the existence of a functional positive feedback loop in which Ckap2 activates the G1 tetraploidy checkpoint and prevents aneuploidy.
Title: Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI461656 (e-PCR)
- UniSTS:159742

AW228814 (e-PCR)
- UniSTS:179711

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mitotic cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of microtubule depolymerization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cytoskeleton-associated protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001004140.2 → NP_001004140.2 cytoskeleton-associated protein 2

See identical proteins and their annotated locations for NP_001004140.2

Status: VALIDATED

Source sequence(s)

AC117665, AK132463, BM217153

Consensus CDS

CCDS22170.1

UniProtKB/Swiss-Prot

A6H5W7, Q3V1H1, Q66LN2, Q8BSF0

Related

ENSMUSP00000039518.8, ENSMUST00000046916.9

Conserved Domains (1) summary

pfam15297
Location:316 → 651

CKAP2_C; Cytoskeleton-associated protein 2 C-terminus

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

22658168..22675835 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006509226.5 → XP_006509289.1 cytoskeleton-associated protein 2 isoform X1

Conserved Domains (1) summary

pfam15297
Location:250 → 584

CKAP2_C; Cytoskeleton-associated protein 2 C-terminus