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Pacsin3 protein kinase C and casein kinase substrate in neurons 3 [ Mus musculus (house mouse) ]

Gene ID: 80708, updated on 30-Apr-2024

Summary

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Official Symbol
Pacsin3provided by MGI
Official Full Name
protein kinase C and casein kinase substrate in neurons 3provided by MGI
Primary source
MGI:MGI:1891410
See related
Ensembl:ENSMUSG00000027257 AllianceGenome:MGI:1891410
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
4921507A02Rik; 6330413E15Rik
Summary
Enables calcium channel inhibitor activity; cytoskeletal protein binding activity; and lipid binding activity. Involved in negative regulation of calcium ion transport and plasma membrane tubulation. Acts upstream of or within negative regulation of endocytosis. Located in cytoplasm. Is expressed in central nervous system and retina. Orthologous to human PACSIN3 (protein kinase C and casein kinase substrate in neurons 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in heart adult (RPKM 20.1), cerebellum adult (RPKM 14.4) and 24 other tissues See more
Orthologs
human all
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Genomic context

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See Pacsin3 in Genome Data Viewer
Location:
2 E1; 2 50.61 cM
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (91086215..91095025)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (91255875..91264680)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA A330069E16 gene Neighboring gene STARR-positive B cell enhancer ABC_E8985 Neighboring gene predicted gene, 31054 Neighboring gene STARR-positive B cell enhancer ABC_E3385 Neighboring gene ADP-ribosylation factor GTPase activating protein 2 Neighboring gene centriolar satellite-associated tubulin polyglutamylase complex regulator 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:91148974-91149175 Neighboring gene predicted gene 13787

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination. Seemann E, et al. Elife, 2017 Dec 5. PMID 29202928, Free PMC Article
  2. Cloning, purification, crystallization and preliminary X-ray diffraction analysis of mouse PACSIN 3 protein. Bai X, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2012 Feb 1. PMID 22297988, Free PMC Article
  3. Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells. Samasilp P, et al. Am J Physiol Cell Physiol, 2014 May 1. PMID 24500282, Free PMC Article
  4. PACSIN3 overexpression increases adipocyte glucose transport through GLUT1. Roach W, et al. Biochem Biophys Res Commun, 2007 Apr 13. PMID 17320047, Free PMC Article
  5. Rigidity of wedge loop in PACSIN 3 protein is a key factor in dictating diameters of tubules. Bai X, et al. J Biol Chem, 2012 Jun 22. PMID 22573331, Free PMC Article

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The authors show that muscle cells of syndapin III knockout mice show severe reductions of caveolae reminiscent of human caveolinopathies. The membrane-shaping protein syndapin III is crucial for caveolar invagination and gene knockout rendered the cells sensitive to membrane tensions.
    Title: Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.
  2. Show that in neuroendocrine chromaffin cells fusion pore expansion and catecholamine release are limited specifically by mutation of syndapin 3.
    Title: Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells.
  3. Rigidity of wedge loop in PACSIN 3 protein is a key factor in dictating diameters of tubules
    Title: Rigidity of wedge loop in PACSIN 3 protein is a key factor in dictating diameters of tubules.
  4. crystal of PACSIN 3 (1-341) diffracted to 2.6 A resolution and belonged to space group P2(1), with unit-cell parameters a = 46.9, b = 54.7, c = 193.7 A, alpha = 90, beta = 96.9, gamma = 90 degrees
    Title: Cloning, purification, crystallization and preliminary X-ray diffraction analysis of mouse PACSIN 3 protein.
  5. These findings suggest a novel function for PACSIN proteins 1, 2, and 3 in dynamic microtubule nucleation.
    Title: PACSIN proteins bind tubulin and promote microtubule assembly.
  6. PACSIN 3 acts as an auxiliary protein of TRPV4 channel that not only affects the channel's subcellular localization but also modulates its function in a stimulus-specific manner.
    Title: Stimulus-specific modulation of the cation channel TRPV4 by PACSIN 3.
  7. These results establish a role for PACSIN3 in regulating glucose uptake in adipocytes via its preferential participation in GLUT1 trafficking.
    Title: PACSIN3 overexpression increases adipocyte glucose transport through GLUT1.
  8. PACSIN 3 specifically affects the endocytosis of TRPV4, thereby modulating the subcellular localization
    Title: PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables calcium channel inhibitor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables cytoskeletal protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables lipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipid binding ISO
Inferred from Sequence Orthology
more info
  
enables phospholipid binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cytoskeleton organization IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of calcium ion transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within negative regulation of endocytosis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of endocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in plasma membrane tubulation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in plasma membrane tubulation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in plasma membrane tubulation ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of membrane protein ectodomain proteolysis ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of endocytosis IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
is_active_in endosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
NOT located_in trans-Golgi network IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
protein kinase C and casein kinase II substrate protein 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001289677.1 → NP_001276606.1  protein kinase C and casein kinase II substrate protein 3

    See identical proteins and their annotated locations for NP_001276606.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 through 4 encode the same protein.
    Source sequence(s)
    AK049166, AK078060, AV333674
    Consensus CDS
    CCDS16429.1
    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Related
    ENSMUSP00000054391.5, ENSMUST00000059566.11
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  2. NM_001289678.1 → NP_001276607.1  protein kinase C and casein kinase II substrate protein 3

    See identical proteins and their annotated locations for NP_001276607.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 through 4 encode the same protein.
    Source sequence(s)
    AF149824, AK049166, AV333674, BY250979
    Consensus CDS
    CCDS16429.1
    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Related
    ENSMUSP00000028694.6, ENSMUST00000028694.12
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  3. NM_028733.4 → NP_083009.1  protein kinase C and casein kinase II substrate protein 3

    See identical proteins and their annotated locations for NP_083009.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript. Variants 1 through 4 encode the same protein.
    Source sequence(s)
    AK049166, AV333674
    Consensus CDS
    CCDS16429.1
    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Related
    ENSMUSP00000106981.3, ENSMUST00000111349.9
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  4. NM_030880.3 → NP_112019.2  protein kinase C and casein kinase II substrate protein 3

    See identical proteins and their annotated locations for NP_112019.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1 through 4 encode the same protein.
    Source sequence(s)
    AK141063, AV333674, BY250979
    Consensus CDS
    CCDS16429.1
    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Related
    ENSMUSP00000129175.2, ENSMUST00000168916.8
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    91086215..91095025
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030252256.2 → XP_030108116.1  protein kinase C and casein kinase II substrate protein 3 isoform X1

    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  2. XM_006500427.5 → XP_006500490.1  protein kinase C and casein kinase II substrate protein 3 isoform X1

    See identical proteins and their annotated locations for XP_006500490.1

    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  3. XM_006500425.1 → XP_006500488.1  protein kinase C and casein kinase II substrate protein 3 isoform X1

    See identical proteins and their annotated locations for XP_006500488.1

    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  4. XM_006500428.5 → XP_006500491.1  protein kinase C and casein kinase II substrate protein 3 isoform X1

    See identical proteins and their annotated locations for XP_006500491.1

    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  5. XM_006500424.5 → XP_006500487.1  protein kinase C and casein kinase II substrate protein 3 isoform X1

    See identical proteins and their annotated locations for XP_006500487.1

    UniProtKB/Swiss-Prot
    Q99JB8, Q9EQP9
    UniProtKB/TrEMBL
    Q543N7
    Conserved Domains (2) summary
    cd11997
    Location:365 → 420
    SH3_PACSIN3; Src homology 3 domain of Protein kinase C and Casein kinase Substrate in Neurons 3 (PACSIN3)
    cl12013
    Location:16 → 273
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q99JB8.1 GenPept UniProtKB/Swiss-Prot:Q99JB8

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