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ZAP70 zeta chain of T cell receptor associated protein kinase 70 [ Homo sapiens (human) ]

Gene ID: 7535, updated on 5-Jul-2020

Summary

Official Symbol
ZAP70provided by HGNC
Official Full Name
zeta chain of T cell receptor associated protein kinase 70provided by HGNC
Primary source
HGNC:HGNC:12858
See related
Ensembl:ENSG00000115085 MIM:176947
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Summary
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression
Biased expression in lymph node (RPKM 11.2), spleen (RPKM 7.9) and 11 other tissues See more
Orthologs

Genomic context

See ZAP70 in Genome Data Viewer
Location:
2q11.2
Exon count:
20
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 2 NC_000002.12 (97712030..97744327)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (98330031..98356990)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene actin related protein 1B Neighboring gene chromosome 2 open reading frame 92 Neighboring gene RNA, U4 small nuclear 8, pseudogene Neighboring gene uncharacterized LOC107985921 Neighboring gene transmembrane protein 131 Neighboring gene high mobility group nucleosome binding domain 1 pseudogene 36

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Binding of HIV-1 gp120 to CD4 induces p56lck activation and zeta-chain (TCR) associated protein kinase 70kDa desensitization independent of TCR tyrosine phosphorylation PubMed
env Lck is recuited to a virological synapse (VS) interface and is highly colocalized with gp120 when cells interact with gp120+ICAM-1 bilayers, leading to activation of ZAP70, LAT, SLP76, and PLCgamma PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 alone or CD4/gp160 cross-linking induces tyrosine phosphorylation of intracellular substrates p59fyn, zap 70, and p95vav and also leads to ras activation PubMed
Nef nef Diaminoquinoxaline benzenesulfonamide (DQBS) treatment reduces the amount of both HCK and the p85 regulatory subunit of PI3K associated with HIV-1 Nef and completely blocks Nef-dependent activation of ZAP-70 PubMed
nef HIV-1 Nef inhibits colocalization of activated ZAP70 with SLP76 in microclusters (MCs) PubMed
nef Nef/Hck complex recruits and phosphorylates the tyrosine kinase ZAP-70, which binds class I PI3K to trigger MHC-I downregulation in primary CD4+ T cells PubMed
Pr55(Gag) gag Intracellular Gag localization is regulated by the presence of ZAP70 in lymphocytes PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Homology

Clone Names

  • FLJ17670, FLJ17679

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding NAS
Non-traceable Author Statement
more info
PubMed 
non-membrane spanning protein tyrosine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
non-membrane spanning protein tyrosine kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
non-membrane spanning protein tyrosine kinase activity TAS
Traceable Author Statement
more info
PubMed 
phosphotyrosine residue binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
phosphotyrosine residue binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein tyrosine kinase activity NAS
Non-traceable Author Statement
more info
PubMed 
protein tyrosine kinase activity TAS
Traceable Author Statement
more info
 
signaling receptor binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Process Evidence Code Pubs
B cell activation TAS
Traceable Author Statement
more info
PubMed 
T cell activation TAS
Traceable Author Statement
more info
PubMed 
T cell aggregation TAS
Traceable Author Statement
more info
PubMed 
T cell differentiation NAS
Non-traceable Author Statement
more info
PubMed 
T cell migration TAS
Traceable Author Statement
more info
PubMed 
T cell receptor signaling pathway TAS
Traceable Author Statement
more info
PubMed 
adaptive immune response TAS
Traceable Author Statement
more info
PubMed 
beta selection IEA
Inferred from Electronic Annotation
more info
 
cell differentiation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
immune response IDA
Inferred from Direct Assay
more info
PubMed 
innate immune response IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
intracellular signal transduction NAS
Non-traceable Author Statement
more info
PubMed 
negative thymic T cell selection IEA
Inferred from Electronic Annotation
more info
 
peptidyl-tyrosine autophosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
peptidyl-tyrosine phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
peptidyl-tyrosine phosphorylation IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of T cell differentiation IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of alpha-beta T cell differentiation IEA
Inferred from Electronic Annotation
more info
 
positive regulation of alpha-beta T cell proliferation IEA
Inferred from Electronic Annotation
more info
 
positive regulation of calcium-mediated signaling IEA
Inferred from Electronic Annotation
more info
 
positive thymic T cell selection IDA
Inferred from Direct Assay
more info
PubMed 
protein autophosphorylation IEA
Inferred from Electronic Annotation
more info
 
protein phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
protein phosphorylation NAS
Non-traceable Author Statement
more info
PubMed 
regulation of cell proliferation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
transmembrane receptor protein tyrosine kinase signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
T cell receptor complex IDA
Inferred from Direct Assay
more info
PubMed 
cell-cell junction IEA
Inferred from Electronic Annotation
more info
 
cytoplasm TAS
Traceable Author Statement
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
extrinsic component of cytoplasmic side of plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
immunological synapse IEA
Inferred from Electronic Annotation
more info
 
colocalizes_with membrane raft IDA
Inferred from Direct Assay
more info
PubMed 
plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
plasma membrane TAS
Traceable Author Statement
more info
PubMed 

General protein information

Preferred Names
tyrosine-protein kinase ZAP-70
Names
70 kDa zeta-associated protein
70 kDa zeta-chain associated protein
syk-related tyrosine kinase
zeta chain of T cell receptor associated protein kinase 70kDa
zeta-chain (TCR) associated protein kinase 70kDa
zeta-chain associated protein kinase, 70kD
NP_001070.2
NP_001365523.1
NP_997402.1
XP_016860356.1
XP_016860357.1
XP_016860358.1
XP_016860359.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_007727.1 RefSeqGene

    Range
    5001..31293
    Download
    GenBank, FASTA, Sequence Viewer (Graphics), LRG_126

mRNA and Protein(s)

  1. NM_001079.4NP_001070.2  tyrosine-protein kinase ZAP-70 isoform 1

    See identical proteins and their annotated locations for NP_001070.2

    Status: REVIEWED

    Source sequence(s)
    AC016699, AC017099
    Consensus CDS
    CCDS33254.1
    UniProtKB/Swiss-Prot
    P43403
    Related
    ENSP00000264972.5, ENST00000264972.9
    Conserved Domains (4) summary
    cd09938
    Location:8111
    SH2_N-SH2_Zap70_Syk_like; N-terminal Src homology 2 (SH2) domain found in Zeta-chain-associated protein kinase 70 (ZAP-70) and Spleen tyrosine kinase (Syk) proteins
    cd10402
    Location:152255
    SH2_C-SH2_Zap70; C-terminal Src homology 2 (SH2) domain found in Zeta-chain-associated protein kinase 70 (ZAP-70)
    cd05115
    Location:333601
    PTKc_Zap-70; Catalytic domain of the Protein Tyrosine Kinase, Zeta-chain-associated protein of 70kDa
    pfam07714
    Location:343593
    Pkinase_Tyr; Protein tyrosine kinase
  2. NM_001378594.1NP_001365523.1  tyrosine-protein kinase ZAP-70 isoform 1

    Status: REVIEWED

    Source sequence(s)
    AC016699, AC017099
  3. NM_207519.2NP_997402.1  tyrosine-protein kinase ZAP-70 isoform 2

    See identical proteins and their annotated locations for NP_997402.1

    Status: REVIEWED

    Source sequence(s)
    AC016699
    Consensus CDS
    CCDS33255.1
    UniProtKB/Swiss-Prot
    P43403
    Related
    ENSP00000400475.2, ENST00000451498.2
    Conserved Domains (1) summary
    cd05115
    Location:26294
    PTKc_Zap-70; Catalytic domain of the Protein Tyrosine Kinase, Zeta-chain-associated protein of 70kDa

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p13 Primary Assembly

    Range
    97712030..97744327
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017004869.1XP_016860358.1  tyrosine-protein kinase ZAP-70 isoform X3

  2. XM_017004867.1XP_016860356.1  tyrosine-protein kinase ZAP-70 isoform X1

  3. XM_017004868.1XP_016860357.1  tyrosine-protein kinase ZAP-70 isoform X2

  4. XM_017004870.1XP_016860359.1  tyrosine-protein kinase ZAP-70 isoform X4

RNA

  1. XR_001738925.1 RNA Sequence

  2. XR_001738926.1 RNA Sequence

  3. XR_001738927.1 RNA Sequence

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