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Med27 mediator complex subunit 27 [ Mus musculus (house mouse) ]

Gene ID: 68975, updated on 2-Nov-2024

Summary

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Official Symbol
Med27provided by MGI
Official Full Name
mediator complex subunit 27provided by MGI
Primary source
MGI:MGI:1916225
See related
Ensembl:ENSMUSG00000026799 AllianceGenome:MGI:1916225
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Crsp8; D2Ertd434e; 1500015J03Rik; 2310042P07Rik
Summary
Predicted to enable transcription coactivator activity. Predicted to contribute to ubiquitin protein ligase activity. Acts upstream of or within somatic stem cell population maintenance. Predicted to be located in cytosol; nucleolus; and nucleoplasm. Predicted to be part of core mediator complex; mediator complex; and ubiquitin ligase complex. Is expressed in genitourinary system and tibialis anterior. Orthologous to human MED27 (mediator complex subunit 27). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in whole brain E14.5 (RPKM 6.7), CNS E14 (RPKM 6.0) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Med27 in Genome Data Viewer
Location:
2 B; 2 19.84 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (29236822..29414802)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (29346810..29524790)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:28980423-28980610 Neighboring gene STARR-positive B cell enhancer ABC_E5902 Neighboring gene STARR-seq mESC enhancer starr_04042 Neighboring gene STARR-seq mESC enhancer starr_04043 Neighboring gene senataxin Neighboring gene STARR-seq mESC enhancer starr_04046 Neighboring gene RIKEN cDNA 6530402F18 gene Neighboring gene netrin G2 Neighboring gene STARR-seq mESC enhancer starr_04048 Neighboring gene STARR-seq mESC enhancer starr_04049 Neighboring gene STARR-positive B cell enhancer ABC_E4423 Neighboring gene STARR-seq mESC enhancer starr_04051 Neighboring gene STARR-seq mESC enhancer starr_04052 Neighboring gene microRNA 133c Neighboring gene STARR-seq mESC enhancer starr_04054 Neighboring gene STARR-positive B cell enhancer ABC_E7802 Neighboring gene predicted gene, 30761 Neighboring gene STARR-seq mESC enhancer starr_04055 Neighboring gene STARR-positive B cell enhancer ABC_E9194 Neighboring gene STARR-positive B cell enhancer ABC_E2607 Neighboring gene STARR-seq mESC enhancer starr_04057 Neighboring gene Rap guanine nucleotide exchange factor (GEF) 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:29526317-29526500 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:29529743-29529852 Neighboring gene STARR-seq mESC enhancer starr_04064 Neighboring gene STARR-positive B cell enhancer ABC_E9506 Neighboring gene STARR-positive B cell enhancer ABC_E2608 Neighboring gene STARR-seq mESC enhancer starr_04069

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. The essential role of MED27 in stabilizing the mediator complex for cardiac development and function. Zhu S, et al. Life Sci, 2024 Nov 1. PMID 39209248
  2. Cloning of wrinkle-free, a previously uncharacterized mouse mutation, reveals crucial roles for fatty acid transport protein 4 in skin and hair development. Moulson CL, et al. Proc Natl Acad Sci U S A, 2003 Apr 29. PMID 12697906, Free PMC Article
  3. Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Kolonay DW, et al. Front Cell Dev Biol, 2024. PMID 38690566, Free PMC Article
  4. Novel critical role of a human Mediator complex for basal RNA polymerase II transcription. Mittler G, et al. EMBO Rep, 2001 Sep. PMID 11559591, Free PMC Article
  5. Mediator and cohesin connect gene expression and chromatin architecture. Kagey MH, et al. Nature, 2010 Sep 23. PMID 20720539, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The essential role of MED27 in stabilizing the mediator complex for cardiac development and function.
    Title: The essential role of MED27 in stabilizing the mediator complex for cardiac development and function.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables transcription coactivator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coactivator activity ISO
Inferred from Sequence Orthology
more info
  
enables transcription coactivator activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables ubiquitin protein ligase activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to ubiquitin protein ligase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in protein ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within somatic stem cell population maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of core mediator complex ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IEA
Inferred from Electronic Annotation
more info
  
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleolus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  
part_of transcription regulator complex ISO
Inferred from Sequence Orthology
more info
  
part_of ubiquitin ligase complex IEA
Inferred from Electronic Annotation
more info
  
part_of ubiquitin ligase complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 27
Names
CRSP complex subunit 8
cofactor required for Sp1 transcriptional activation, subunit 8
transcriptional co-activator CRSP8 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001290489.1 → NP_001277418.1  mediator of RNA polymerase II transcription subunit 27 isoform c

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks two 5' exons but instead contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a.
    Source sequence(s)
    AI850753, AL772213, AL929437, CJ073494
    Consensus CDS
    CCDS79764.1
    UniProtKB/TrEMBL
    E0CZG8
    Related
    ENSMUSP00000125390.2, ENSMUST00000159280.8
    Conserved Domains (1) summary
    pfam11571
    Location:89 → 170
    Med27; Mediator complex subunit 27

  2. NM_001290490.1 → NP_001277419.1  mediator of RNA polymerase II transcription subunit 27 isoform d

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) contains alternate 5' and 3' exon structure, and it thus differs in both UTRs and in the 5' and 3' coding regions, compared to variant 1. The encoded isoform (d) has distinct N- and C-termini and is shorter than isoform a.
    Source sequence(s)
    AK147181, AL929437, CJ073494, DV648756
    UniProtKB/Swiss-Prot
    Q9DB40

  3. NM_026896.5 → NP_081172.2  mediator of RNA polymerase II transcription subunit 27 isoform a

    See identical proteins and their annotated locations for NP_081172.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (a).
    Source sequence(s)
    AI850753, AK005251, CJ072886
    Consensus CDS
    CCDS15853.1
    UniProtKB/Swiss-Prot
    A2AJV2, Q78JI3, Q9D707, Q9DB40
    Related
    ENSMUSP00000028139.5, ENSMUST00000028139.11
    Conserved Domains (1) summary
    pfam11571
    Location:228 → 306
    Med27; Mediator complex subunit 27

  4. NM_029615.1 → NP_083891.1  mediator of RNA polymerase II transcription subunit 27 isoform b

    See identical proteins and their annotated locations for NP_083891.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks four 3' exons but instead contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a.
    Source sequence(s)
    AK009764, AK147181, AL929437, CJ072886
    Consensus CDS
    CCDS71014.1
    UniProtKB/Swiss-Prot
    Q9DB40
    Related
    ENSMUSP00000109461.5, ENSMUST00000113830.11

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    29236822..29414802
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017319247.3 → XP_017174736.1  mediator of RNA polymerase II transcription subunit 27 isoform X2

  2. XM_017319246.1 → XP_017174735.1  mediator of RNA polymerase II transcription subunit 27 isoform X1

    Conserved Domains (1) summary
    pfam11571
    Location:115 → 194
    Med27; Mediator complex subunit 27
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9DB40.2 GenPept UniProtKB/Swiss-Prot:Q9DB40

Gene LinkOut

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