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MED27 mediator complex subunit 27 [ Homo sapiens (human) ]

Gene ID: 9442, updated on 25-Nov-2021

Summary

Official Symbol
MED27provided by HGNC
Official Full Name
mediator complex subunit 27provided by HGNC
Primary source
HGNC:HGNC:2377
See related
Ensembl:ENSG00000160563 MIM:605044
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MED3; CRSP8; CRAP34; CRSP34; TRAP37; NEDSCAC
Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
Expression
Ubiquitous expression in testis (RPKM 2.5), brain (RPKM 2.0) and 25 other tissues See more
Orthologs
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Genomic context

See MED27 in Genome Data Viewer
Location:
9q34.13
Exon count:
11
Annotation release Status Assembly Chr Location
109.20211119 current GRCh38.p13 (GCF_000001405.39) 9 NC_000009.12 (131860110..132079867, complement)
105.20201022 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (134735499..134955254, complement)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene Rap guanine nucleotide exchange factor 1 Neighboring gene Sharpr-MPRA regulatory region 10210 Neighboring gene eukaryotic translation initiation factor 4A1 pseudogene 3 Neighboring gene uncharacterized LOC105376302 Neighboring gene Sharpr-MPRA regulatory region 1592 Neighboring gene netrin G2

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 27 (MED27) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat Knockdown of MED27 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ42238, MGC11274

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables transcription coactivator activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
PubMed 
enables ubiquitin protein ligase activity IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
part_of core mediator complex IPI
Inferred from Physical Interaction
more info
PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 
located_in nucleoplasm TAS
Traceable Author Statement
more info
 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
part_of transcription regulator complex IDA
Inferred from Direct Assay
more info
PubMed 
part_of ubiquitin ligase complex IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
mediator of RNA polymerase II transcription subunit 27
Names
CRSP complex subunit 8
cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
epididymis secretory sperm binding protein
p37 TRAP/SMCC/PC2 subunit
transcriptional coactivator CRSP34

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001253881.2NP_001240810.1  mediator of RNA polymerase II transcription subunit 27 isoform 2

    See identical proteins and their annotated locations for NP_001240810.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. the encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    BC002878, BP255072
    Consensus CDS
    CCDS59153.1
    UniProtKB/Swiss-Prot
    Q6P2C8
    Related
    ENSP00000349530.3, ENST00000357028.6
    Conserved Domains (1) summary
    pfam11571
    Location:192273
    Med27; Mediator complex subunit 27
  2. NM_001253882.2NP_001240811.1  mediator of RNA polymerase II transcription subunit 27 isoform 3

    See identical proteins and their annotated locations for NP_001240811.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate terminal exon and lacks a large portion of the 3' coding region, compared to variant 1. The encoded isoform (3) is significantly shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    BP255072, BU069864, CD388680
    Consensus CDS
    CCDS69689.1
    UniProtKB/Swiss-Prot
    Q6P2C8
    Related
    ENSP00000477136.1, ENST00000474263.1
  3. NM_004269.4NP_004260.2  mediator of RNA polymerase II transcription subunit 27 isoform 1

    See identical proteins and their annotated locations for NP_004260.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    BC064608, BP255072
    Consensus CDS
    CCDS6945.1
    UniProtKB/Swiss-Prot
    Q6P2C8
    UniProtKB/TrEMBL
    A0A024R8B7
    Related
    ENSP00000292035.5, ENST00000292035.10
    Conserved Domains (1) summary
    pfam11571
    Location:228309
    Med27; Mediator complex subunit 27

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20211119

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p13 Primary Assembly

    Range
    131860110..132079867 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017015329.1XP_016870818.1  mediator of RNA polymerase II transcription subunit 27 isoform X1

  2. XM_005272236.3XP_005272293.1  mediator of RNA polymerase II transcription subunit 27 isoform X3

  3. XM_017015330.2XP_016870819.1  mediator of RNA polymerase II transcription subunit 27 isoform X2

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