Wls wntless WNT ligand secretion mediator [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Wlsprovided by MGI
Official Full Name: wntless WNT ligand secretion mediatorprovided by MGI
Primary source: MGI:MGI:1915401
See related: Ensembl:ENSMUSG00000028173 AllianceGenome:MGI:1915401
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EVI; Gpr177; 5031439A09Rik
Summary: Enables Wnt-protein binding activity. Involved in several processes, including brain development; cementum mineralization; and exocrine pancreas development. Acts upstream of or within Wnt protein secretion. Located in several cellular components, including Golgi apparatus; cytoplasmic vesicle; and endoplasmic reticulum. Is expressed in several structures, including alimentary system; brain; respiratory system; sensory organ; and skin. Used to study Zaki syndrome. Human ortholog(s) of this gene implicated in Zaki syndrome. Orthologous to human WLS (Wnt ligand secretion mediator). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Broad expression in bladder adult (RPKM 41.9), limb E14.5 (RPKM 30.9) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 H4; 3 82.65 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (159545287..159644302)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (159839650..159938666)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation.
Title: GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation.
Epithelial Wntless regulates postnatal alveologenesis.
Title: Epithelial Wntless regulates postnatal alveologenesis.
Yap Promotes Noncanonical Wnt Signals From Cardiomyocytes for Heart Regeneration.
Title: Yap Promotes Noncanonical Wnt Signals From Cardiomyocytes for Heart Regeneration.
The function of Wls in ovarian development.
Title: The function of Wls in ovarian development.
Wntless regulates lipogenic gene expression in adipocytes and protects against diet-induced metabolic dysfunction.
Title: Wntless regulates lipogenic gene expression in adipocytes and protects against diet-induced metabolic dysfunction.
Conditional deletion of Wntless in granulosa cells causes impaired corpora lutea formation and subfertility.
Title: Conditional deletion of Wntless in granulosa cells causes impaired corpora lutea formation and subfertility.
Data show that epithelial Wntless was required for the growth and differentiation of small intestinal organoids but not in live animals.
Title: Epithelial Wntless is dispensable for intestinal tumorigenesis in mouse models.
Deletion of WLS in peritubular myoid cells is not essential for male fertility in mice.
Title: Selective deletion of WLS in peritubular myoid cells does not affect spermatogenesis or fertility in mice.
Data show that Shh-Cre-mediated deletion of Wntless, the Wnt cargo protein, in mouse posterior limb mesenchyme causes bone syndactyly of the 3rd and 4th digits, resembling the human Malik-Percin type . Wntless(Shh-Cre) limbs displayed altered expression of hedgehog pathway genes and impaired noncanonical Wnt signaling activity.
Title: Disruption of Wnt production in Shh lineage causes bone malformation in mice, mimicking human Malik-Percin-type syndactyly.
WLS role in the principal cells of the caput epididymidis during sperm maturation
Title: Role of WNT signaling in epididymal sperm maturation.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation
Gene trapped (1)
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI987742 (e-PCR)
- UniSTS:178961

AI173978 (e-PCR)
- UniSTS:159209

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables Wnt-protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables Wnt-protein binding	IDA Inferred from Direct Assay more info	PubMed
enables Wnt-protein binding	IEA Inferred from Electronic Annotation more info
enables Wnt-protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables mu-type opioid receptor binding	IEA Inferred from Electronic Annotation more info
enables mu-type opioid receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Wnt protein secretion	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Wnt protein secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in Wnt protein secretion	IEA Inferred from Electronic Annotation more info
involved_in Wnt protein secretion	ISO Inferred from Sequence Orthology more info
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in anterior/posterior axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cementum mineralization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocrine pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hindbrain development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in intracellular protein transport	ISO Inferred from Sequence Orthology more info
involved_in mesoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in midbrain development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Wnt protein secretion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Wnt protein secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Wnt protein secretion	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Wnt signaling pathway	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of canonical Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite cytoplasm	IEA Inferred from Electronic Annotation more info
located_in dendrite cytoplasm	ISO Inferred from Sequence Orthology more info
located_in dendrite membrane	IEA Inferred from Electronic Annotation more info
located_in dendrite membrane	ISO Inferred from Sequence Orthology more info
located_in early endosome	IEA Inferred from Electronic Annotation more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in endomembrane system	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in organelle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
located_in trans-Golgi network	IEA Inferred from Electronic Annotation more info
located_in trans-Golgi network	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein wntless homolog

Names: G protein-coupled receptor 177; integral membrane protein GPR177; protein evenness interrupted homolog; wntless homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001356349.1 → NP_001343278.1 protein wntless homolog isoform 2 precursor

Status: VALIDATED

Source sequence(s)

AC121906, AC225719, AK019886, BQ179316

Conserved Domains (1) summary

pfam06664
Location:78 → 396

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion
NM_001356350.1 → NP_001343279.1 protein wntless homolog isoform 1

Status: VALIDATED

Source sequence(s)

AC121906, AC175245, AC225719, AK044810

Consensus CDS

CCDS38684.1

UniProtKB/Swiss-Prot

Q6DID7, Q8CE42, Q9D2B7

Related

ENSMUSP00000143475.2, ENSMUST00000198878.2

Conserved Domains (1) summary

pfam06664
Location:178 → 496

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion
NM_026582.5 → NP_080858.3 protein wntless homolog isoform 1

See identical proteins and their annotated locations for NP_080858.3

Status: VALIDATED

Source sequence(s)

AC121906, AC225719, BC075615

Consensus CDS

CCDS38684.1

UniProtKB/Swiss-Prot

Q6DID7, Q8CE42, Q9D2B7

Related

ENSMUSP00000067898.6, ENSMUST00000068952.10

Conserved Domains (1) summary

pfam06664
Location:178 → 496

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion