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SPAG4 sperm associated antigen 4 [ Homo sapiens (human) ]

Gene ID: 6676, updated on 10-Dec-2024

Summary

Official Symbol
SPAG4provided by HGNC
Official Full Name
sperm associated antigen 4provided by HGNC
Primary source
HGNC:HGNC:11214
See related
Ensembl:ENSG00000061656 MIM:603038; AllianceGenome:HGNC:11214
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
SUN4; CT127
Summary
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Expression
Broad expression in testis (RPKM 8.6), pancreas (RPKM 6.3) and 20 other tissues See more
Orthologs
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Genomic context

See SPAG4 in Genome Data Viewer
Location:
20q11.22
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 20 NC_000020.11 (35615829..35621094)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 20 NC_060944.1 (37336805..37342070)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 20 NC_000020.10 (34203751..34209016)

Chromosome 20 - NC_000020.11Genomic Context describing neighboring genes Neighboring gene fer-1 like family member 4 (pseudogene) Neighboring gene H3K27ac hESC enhancer GRCh37_chr20:34176389-34176889 Neighboring gene H3K27ac hESC enhancer GRCh37_chr20:34177977-34178478 Neighboring gene H3K27ac hESC enhancer GRCh37_chr20:34178479-34178978 Neighboring gene ribosomal protein L37 pseudogene 1 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr20:34187074-34187574 Neighboring gene uncharacterized LOC124904891 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12860 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12861 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 17787 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr20:34205257-34206207 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr20:34206208-34207157 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 17789 Neighboring gene H3K27ac hESC enhancer GRCh37_chr20:34223677-34224176 Neighboring gene copine 1 Neighboring gene RN7SK pseudogene 271 Neighboring gene RNA, U6 small nuclear 759, pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein-membrane adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables structural molecule activity TAS
Traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
involved_in cell differentiation IEA
Inferred from Electronic Annotation
more info
 
involved_in spermatogenesis IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
 
part_of meiotic nuclear membrane microtubule tethering complex IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in motile cilium IEA
Inferred from Electronic Annotation
more info
 
is_active_in nuclear envelope IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in nuclear inner membrane IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
sperm-associated antigen 4 protein
Names
SUN domain-containing protein 4
Sad1 and UNC84 domain containing 4
acrosomal protein ACR55
cancer/testis antigen 127
outer dense fiber-associated protein SPAG4
sperm tail protein

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001317931.1NP_001304860.1  sperm-associated antigen 4 protein isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate 5' terminal exon, resulting in a distinct 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus, and is shorter, compared to isoform 1.
    Source sequence(s)
    BC143783, HY021678
    Consensus CDS
    CCDS93032.1
    UniProtKB/TrEMBL
    A0A7P0Z4G5
    Related
    ENSP00000505928.1, ENST00000679710.1
    Conserved Domains (1) summary
    pfam07738
    Location:214345
    Sad1_UNC; Sad1 / UNC-like C-terminal
  2. NM_003116.3NP_003107.1  sperm-associated antigen 4 protein isoform 1

    See identical proteins and their annotated locations for NP_003107.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AF262992, AI168233, AL109827, HY021678
    Consensus CDS
    CCDS13259.1
    UniProtKB/Swiss-Prot
    O43648, Q9NPE6
    Related
    ENSP00000363391.3, ENST00000374273.8
    Conserved Domains (1) summary
    pfam07738
    Location:291422
    Sad1_UNC; Sad1 / UNC-like C-terminal

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000020.11 Reference GRCh38.p14 Primary Assembly

    Range
    35615829..35621094
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011529009.3XP_011527311.1  sperm-associated antigen 4 protein isoform X1

    Conserved Domains (1) summary
    pfam07738
    Location:247378
    Sad1_UNC; Sad1 / UNC-like C-terminal
  2. XM_005260520.5XP_005260577.1  sperm-associated antigen 4 protein isoform X2

    Conserved Domains (1) summary
    pfam07738
    Location:198329
    Sad1_UNC; Sad1 / UNC-like C-terminal

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060944.1 Alternate T2T-CHM13v2.0

    Range
    37336805..37342070
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054323894.1XP_054179869.1  sperm-associated antigen 4 protein isoform X1

  2. XM_054323895.1XP_054179870.1  sperm-associated antigen 4 protein isoform X2