Spag4 sperm associated antigen 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spag4provided by MGI
Official Full Name: sperm associated antigen 4provided by MGI
Primary source: MGI:MGI:2444120
See related: Ensembl:ENSMUSG00000038180 AllianceGenome:MGI:2444120
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sun4; MNCb-0953; mKIAA4118; 1700041K21Rik
Summary: Predicted to enable identical protein binding activity and protein-membrane adaptor activity. Predicted to be involved in nuclear envelope organization. Predicted to act upstream of or within cell differentiation and spermatogenesis. Predicted to be located in microtubule. Predicted to be part of meiotic nuclear membrane microtubule tethering complex. Predicted to be active in nuclear envelope. Is expressed in brainstem; genitourinary system; and submandibular gland. Orthologous to human SPAG4 (sperm associated antigen 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 206.1) and CNS E14 (RPKM 2.7) See more
Orthologs: human all
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Genomic context

Location:: 2; 2 H1

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (155907108..155911421)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (156065188..156069501)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The results thus indicate that SPAG4, albeit not essential for the formation of the HTCA per se, is, nevertheless, required for tightening the sperm head-to-tail anchorage by provoking the correct attachment of the lateral parts of the basal plate to the implantation fossa.
Title: Ultra-structure of the sperm head-to-tail linkage complex in the absence of the spermatid-specific LINC component SPAG4.
SUN4, a spermatid nuclear membrane protein has an essential function in coupling the manchette to the nuclear periphery.
Title: SUN4 is essential for nuclear remodeling during mammalian spermiogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the Spag4 gene: The Spag4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, and rat.
Orthologs from Annotation Pipeline: 198 organisms have orthologs with human gene Spag4
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein-membrane adaptor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spermatogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cilium	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of meiotic nuclear membrane microtubule tethering complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in microtubule	ISO Inferred from Sequence Orthology more info
located_in motile cilium	IEA Inferred from Electronic Annotation more info
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sperm-associated antigen 4 protein

Names: SUN domain-containing protein 4; outer dense fiber-associated protein SPAG4; sperm antigen 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_139151.4 → NP_631890.3 sperm-associated antigen 4 protein

See identical proteins and their annotated locations for NP_631890.3

Status: VALIDATED

Source sequence(s)

AK028080, AK043812, BY231113, BY591699

Consensus CDS

CCDS50777.1

UniProtKB/Swiss-Prot

A3KGK4, Q9JJF2

UniProtKB/TrEMBL

Q8BLQ4

Related

ENSMUSP00000036484.6, ENSMUST00000038860.12

Conserved Domains (1) summary

pfam07738
Location:293 → 424

Sad1_UNC; Sad1 / UNC-like C-terminal

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

155907108..155911421

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006499540.4 → XP_006499603.1 sperm-associated antigen 4 protein isoform X1

Conserved Domains (2) summary

pfam07738
Location:214 → 345

Sad1_UNC; Sad1 / UNC-like C-terminal

cl21464
Location:5 → 74

cupin_like; Conserved domain found in cupin and related proteins