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Spc25 SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) [ Mus musculus (house mouse) ]

Gene ID: 66442, updated on 30-Apr-2024

Summary

Official Symbol
Spc25provided by MGI
Official Full Name
SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)provided by MGI
Primary source
MGI:MGI:1913692
See related
Ensembl:ENSMUSG00000005233 AllianceGenome:MGI:1913692
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Spbc25; 2600017H08Rik; 2610205L13Rik
Summary
This gene encodes a component of the kinetochore-associated NDC80 protein complex, which is required for the mitotic spindle checkpoint and for microtubule-kinetochore attachment. During meiosis in mouse, the protein localizes to the germinal vesicle and then is associated with the chromosomes following germinal vesicle breakdown. Knockdown of this gene in oocytes results in precocious polar body extrusion, chromosome misalignment and aberrant spindle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Expression
Biased expression in liver E14 (RPKM 25.3), CNS E11.5 (RPKM 24.7) and 10 other tissues See more
Orthologs
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Genomic context

See Spc25 in Genome Data Viewer
Location:
2 C2; 2 39.64 cM
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (69009753..69036583, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (69193895..69206213, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene ceramide synthase 6 Neighboring gene predicted gene, 52515 Neighboring gene STARR-seq mESC enhancer starr_04722 Neighboring gene STARR-seq mESC enhancer starr_04727 Neighboring gene predicted gene, 39840 Neighboring gene nitric oxide synthase trafficker Neighboring gene STARR-positive B cell enhancer ABC_E412 Neighboring gene STARR-seq mESC enhancer starr_04729 Neighboring gene glucose-6-phosphatase, catalytic, 2 Neighboring gene STARR-seq mESC enhancer starr_04731 Neighboring gene ATP-binding cassette, sub-family B member 11 Neighboring gene STARR-seq mESC enhancer starr_04732

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
 
Process Evidence Code Pubs
involved_in attachment of spindle microtubules to kinetochore ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within cell cycle IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within cell division IEA
Inferred from Electronic Annotation
more info
 
involved_in chromosome segregation IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in chromosome segregation ISO
Inferred from Sequence Orthology
more info
 
involved_in chromosome segregation NAS
Non-traceable Author Statement
more info
PubMed 
involved_in mitotic spindle assembly checkpoint signaling NAS
Non-traceable Author Statement
more info
PubMed 
involved_in mitotic spindle organization ISO
Inferred from Sequence Orthology
more info
 
Component Evidence Code Pubs
part_of Ndc80 complex IBA
Inferred from Biological aspect of Ancestor
more info
 
part_of Ndc80 complex ISO
Inferred from Sequence Orthology
more info
 
located_in chromosome IEA
Inferred from Electronic Annotation
more info
 
located_in chromosome, centromeric region IEA
Inferred from Electronic Annotation
more info
 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
 
located_in kinetochore ISO
Inferred from Sequence Orthology
more info
 
located_in kinetochore NAS
Non-traceable Author Statement
more info
PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
kinetochore protein Spc25
Names
spindle pole body component 25 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001199123.2NP_001186052.1  kinetochore protein Spc25 isoform 1

    See identical proteins and their annotated locations for NP_001186052.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, and 3 encode the same isoform.
    Source sequence(s)
    AL929221, BC033605, CJ086993
    Consensus CDS
    CCDS57174.1
    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Related
    ENSMUSP00000107939.2, ENSMUST00000112320.8
    Conserved Domains (2) summary
    COG4942
    Location:55129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151220
    Spindle_Spc25; Chromosome segregation protein Spc25
  2. NM_001199124.2NP_001186053.1  kinetochore protein Spc25 isoform 1

    See identical proteins and their annotated locations for NP_001186053.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
    Source sequence(s)
    AL929221, BC027121, CJ086993, CX222514
    Consensus CDS
    CCDS57174.1
    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Related
    ENSMUSP00000005365.9, ENSMUST00000005365.15
    Conserved Domains (2) summary
    COG4942
    Location:55129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151220
    Spindle_Spc25; Chromosome segregation protein Spc25
  3. NM_001305800.1NP_001292729.1  kinetochore protein Spc25 isoform 1

    See identical proteins and their annotated locations for NP_001292729.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
    Source sequence(s)
    AK146727, AL929221, BC033605, CJ086993
    Consensus CDS
    CCDS57174.1
    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Conserved Domains (2) summary
    COG4942
    Location:55129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151220
    Spindle_Spc25; Chromosome segregation protein Spc25
  4. NM_025565.4NP_079841.1  kinetochore protein Spc25 isoform 2

    See identical proteins and their annotated locations for NP_079841.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform (2), which has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AK003408, AL929221, BC033605, CJ086993, CX222514
    Consensus CDS
    CCDS16088.1
    UniProtKB/TrEMBL
    A2AUT4
    Related
    ENSMUSP00000128039.3, ENSMUST00000167875.9
    Conserved Domains (1) summary
    pfam08234
    Location:103172
    Spindle_Spc25; Chromosome segregation protein Spc25

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    69009753..69036583 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036162451.1XP_036018344.1  kinetochore protein Spc25 isoform X2

    UniProtKB/TrEMBL
    A2AUT3
    Conserved Domains (1) summary
    COG4942
    Location:55129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
  2. XM_006500003.4XP_006500066.1  kinetochore protein Spc25 isoform X1

    See identical proteins and their annotated locations for XP_006500066.1

    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Conserved Domains (2) summary
    COG4942
    Location:55129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151220
    Spindle_Spc25; Chromosome segregation protein Spc25
  3. XM_036162450.1XP_036018343.1  kinetochore protein Spc25 isoform X1

    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Conserved Domains (2) summary
    COG4942
    Location:55129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151220
    Spindle_Spc25; Chromosome segregation protein Spc25