Mcm3ap minichromosome maintenance complex component 3 associated protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mcm3approvided by MGI
Official Full Name: minichromosome maintenance complex component 3 associated proteinprovided by MGI
Primary source: MGI:MGI:1930089
See related: Ensembl:ENSMUSG00000001150 AllianceGenome:MGI:1930089
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GANP
Summary: Predicted to enable H3 histone acetyltransferase activity; chromatin binding activity; and histone binding activity. Predicted to be involved in nucleosome organization; poly(A)+ mRNA export from nucleus; and somatic hypermutation of immunoglobulin genes. Predicted to act upstream of or within immune system process; mRNA transport; and protein transport. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; genitourinary system; heart; nervous system; and sensory organ. Orthologous to human MCM3AP (minichromosome maintenance complex component 3 associated protein). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 11.2), CNS E14 (RPKM 10.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 38.88 cM

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (76304736..76351693)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (76468902..76515859)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GANP works to maintain antibody structure by reducing drastic changes in the IgV framework regions in affinity maturation
Title: Integrity of immunoglobulin variable regions is supported by GANP during AID-induced somatic hypermutation in germinal center B cells.
These results indicated that the GANP protein is associated with breast cancer resistance.
Title: GANP protein encoded on human chromosome 21/mouse chromosome 10 is associated with resistance to mammary tumor development.
GANP plays a positive role in IgV region diversification of DT40 B cells in a nonhomologous end joining-proficient state. DNA-PKcs physically interacts with GANP, and this interaction is dissociated by dsDNA breaks
Title: GANP regulates the choice of DNA repair pathway by DNA-PKcs interaction in AID-dependent IgV region diversification.
GANP-mediated chromatin modification promotes transcription complex recruitment and positioning at immunoglobulin variable loci to favour AID targeting.
Title: GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.
GANP may serve as an essential link required to transport AID to B-cell nuclei and to target AID to actively transcribed IgV regions
Title: GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA.
GANP has a critical role in suppression of DNA hyper-recombination in mammalian cells
Title: GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells.
interaction with MCM3 is associated with a novel phosphatase component G5PR
Title: MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR.
GANP(hi)plasma-like cells appear in lymph nodes of autoimmune mice during aging
Title: Spontaneous increase of plasma-like cells with high GANP expression in the extrafollicular region of lymphoid organs of autoimmune-prone mice.
GANP is involved in V region alteration generating high-affinity Ab.
Title: Generation of high-affinity antibody against T cell-dependent antigen in the Ganp gene-transgenic mouse.
role in generation or maintenance of B cells with high-affinity B cell antigen receptors during the maturation in germinal centers
Title: Germinal center-associated nuclear protein contributes to affinity maturation of B cell antigen receptor in T cell-dependent responses.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Mcm3ap (e-PCR), detects polymorphism
- UniSTS:259105

Mcm3ap (e-PCR), detects polymorphism
- UniSTS:259633

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables histone H3 acetyltransferase activity	ISO Inferred from Sequence Orthology more info
enables histone acetyltransferase activity	ISO Inferred from Sequence Orthology more info
enables histone binding	ISO Inferred from Sequence Orthology more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
involved_in mRNA export from nucleus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in nucleosome organization	ISO Inferred from Sequence Orthology more info
involved_in poly(A)+ mRNA export from nucleus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
involved_in somatic hypermutation of immunoglobulin genes	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
part_of nuclear pore	IEA Inferred from Electronic Annotation more info
part_of nuclear pore nuclear basket	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription export complex 2	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription export complex 2	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: germinal-center associated nuclear protein

Names: 80 kDa MCM3-associated protein; GC-associated DNA primase; minichromosome maintenance deficient 3-associated protein; nuclear protein GANP

NP_062307.2

EC 2.3.1.48

XP_006513957.1

EC 2.3.1.48

XP_036011786.1

EC 2.3.1.48

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019434.3 → NP_062307.2 germinal-center associated nuclear protein

See identical proteins and their annotated locations for NP_062307.2

Status: VALIDATED

Source sequence(s)

BC052452, BC067414, BY004673

Consensus CDS

CCDS23947.1

UniProtKB/Swiss-Prot

Q7TS87, Q9WUU9

UniProtKB/TrEMBL

Q80TX5

Related

ENSMUSP00000125960.2, ENSMUST00000170795.3

Conserved Domains (6) summary

cd12443
Location:429 → 500

RRM_MCM3A_like; RNA recognition motif in 80 kDa MCM3-associated protein (Map80) and similar proteins

pfam03399
Location:679 → 903

SAC3_GANP; SAC3/GANP/Nin1/mts3/eIF-3 p25 family

pfam13634
Location:36 → 117

Nucleoporin_FG; Nucleoporin FG repeat region

pfam16766
Location:1157 → 1226

CID_GANP; Binding region of GANP to ENY2

pfam16768
Location:3 → 286

NupH_GANP; Nucleoporin homology of Germinal-centre associated nuclear protein

pfam16769
Location:1254 → 1967

MCM3AP_GANP; MCM3AP domain of GANP

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

76304736..76351693

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155893.1 → XP_036011786.1 germinal-center associated nuclear protein isoform X1

UniProtKB/TrEMBL

Q8C777

Conserved Domains (3) summary

pfam03399
Location:1 → 229

SAC3_GANP; SAC3/GANP family

pfam16766
Location:464 → 533

CID_GANP; Binding region of GANP to ENY2

pfam16769
Location:561 → 1274

MCM3AP_GANP; MCM3AP domain of GANP
XM_006513894.3 → XP_006513957.1 germinal-center associated nuclear protein isoform X1

UniProtKB/TrEMBL

Q8C777

Conserved Domains (3) summary

pfam03399
Location:1 → 229

SAC3_GANP; SAC3/GANP family

pfam16766
Location:464 → 533

CID_GANP; Binding region of GANP to ENY2

pfam16769
Location:561 → 1274

MCM3AP_GANP; MCM3AP domain of GANP