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DCAF8 DDB1 and CUL4 associated factor 8 [ Homo sapiens (human) ]

Gene ID: 50717, updated on 5-Apr-2020

Summary

Official Symbol
DCAF8provided by HGNC
Official Full Name
DDB1 and CUL4 associated factor 8provided by HGNC
Primary source
HGNC:HGNC:24891
See related
Ensembl:ENSG00000132716 MIM:615820
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
GAN2; H326; WDR42A
Summary
This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2009]
Expression
Ubiquitous expression in thyroid (RPKM 31.0), brain (RPKM 23.6) and 25 other tissues See more
Orthologs

Genomic context

See DCAF8 in Genome Data Viewer
Location:
1q23.2
Exon count:
14
Annotation release Status Assembly Chr Location
109.20200228 current GRCh38.p13 (GCF_000001405.39) 1 NC_000001.11 (160215720..160262549, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (160185505..160232350, complement)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene calsequestrin 1 Neighboring gene uncharacterized LOC729867 Neighboring gene proliferation and apoptosis adaptor protein 15 Neighboring gene uncharacterized LOC100287049 Neighboring gene ribosomal protein SA pseudogene 18 Neighboring gene uncharacterized LOC107985219 Neighboring gene peroxisomal biogenesis factor 19

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Vpr vpr HIV-1 Vpr is identified to have a physical interaction with DDB1 and CUL4 associated factor 8 (DCAF8; WDR42A) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Potential readthrough

Included gene: PEX19

Homology

Clone Names

  • FLJ35857, MGC99640, MGC117276, MGC118891, DKFZp781G1096

Gene Ontology Provided by GOA

Function Evidence Code Pubs
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
post-translational protein modification TAS
Traceable Author Statement
more info
 
protein ubiquitination IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
Cul4-RING E3 ubiquitin ligase complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Cul4-RING E3 ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
 
mitochondrion IDA
Inferred from Direct Assay
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
DDB1- and CUL4-associated factor 8
Names
WD repeat domain 42A
WD repeat-containing protein 42A

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_034154.1 RefSeqGene

    Range
    5001..51846
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_015726.4NP_056541.2  DDB1- and CUL4-associated factor 8

    See identical proteins and their annotated locations for NP_056541.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the functional protein.
    Source sequence(s)
    AL139011
    Consensus CDS
    CCDS1200.1
    UniProtKB/Swiss-Prot
    Q5TAQ9
    UniProtKB/TrEMBL
    B7Z8C9
    Related
    ENSP00000357053.1, ENST00000368074.6
    Conserved Domains (2) summary
    sd00039
    Location:198234
    7WD40; WD40 repeat [structural motif]
    cl25539
    Location:189506
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

RNA

  1. NR_028103.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate 5' exon, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is expected to inhibit the translation of the longest ORF; translation of the upstream ORF would result in nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AL139011
  2. NR_028104.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains an alternate 5' exon and lacks an internal exon, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is expected to inhibit the translation of the longest ORF; translation of the upstream ORF would result in nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AL139011
  3. NR_028105.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) contains an alternate exon in the 5' region and differs in the presence and absence of exons in the 3' region, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is expected to inhibit the translation of the longest ORF; translation of the upstream ORF would result in nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    BC111063, DC296401
    Related
    ENST00000610139.5
  4. NR_028106.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) contains an alternate exon and uses an alternate splice site in the 5' region, and differs in the presence and absence of exons in the 3' region, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is expected to inhibit the translation of the longest ORF; translation of the upstream ORF would result in nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    BC013107, BC111063, BP359560
    Related
    ENST00000475733.5

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p13 Primary Assembly

    Range
    160215720..160262549 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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