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IFI16 interferon gamma inducible protein 16 [ Homo sapiens (human) ]

Gene ID: 3428, updated on 15-May-2022

Summary

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Official Symbol
IFI16provided by HGNC
Official Full Name
interferon gamma inducible protein 16provided by HGNC
Primary source
HGNC:HGNC:5395
See related
Ensembl:ENSG00000163565 MIM:147586; AllianceGenome:HGNC:5395
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PYHIN2; IFNGIP1
Summary
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
Expression
Ubiquitous expression in lymph node (RPKM 45.3), appendix (RPKM 41.3) and 24 other tissues See more
Orthologs
all
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Genomic context

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See IFI16 in Genome Data Viewer
Location:
1q23.1
Exon count:
14
Annotation release Status Assembly Chr Location
110 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (158999976..159055151)
110 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (158137044..158192189)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (158969766..159024941)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene pyrin and HIN domain family member 5, pseudogene Neighboring gene pyrin and HIN domain family member 1 Neighboring gene uncharacterized LOC124904432 Neighboring gene pyrin domain containing 5 Neighboring gene absent in melanoma 2 Neighboring gene RAD1 pseudogene 2 Neighboring gene tRNA-Arg (anticodon TCT) 4-1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Ka NL, et al. Cell Rep, 2021 Dec 21. PMID 34936865
  2. IFI16 Isoforms with Cytoplasmic and Nuclear Locations Play Differential Roles in Recognizing Invaded DNA Viruses. Li D, et al. J Immunol, 2021 Dec 1. PMID 34750204
  3. Increased expression of IFI16 predicts adverse prognosis in multiple myeloma. Huang W, et al. Pharmacogenomics J, 2021 Aug. PMID 33712724
  4. IFI16 contributes to the pathogenesis of abdominal aortic aneurysm by regulating the caspase-1/IL-1β/MCPIP1 pathway. Xue M, et al. Life Sci, 2021 Jan 15. PMID 33188834
  5. IFI16 promotes cervical cancer progression by upregulating PD-L1 in immunomicroenvironment through STING-TBK1-NF-kB pathway. Cai H, et al. Biomed Pharmacother, 2020 Mar. PMID 31896065

See all (199) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. IFI16 induces inflammation in hepatitis B virus-associated glomerulonephritis by regulating the Caspase-1/ IL-1 ss pathway.
    Title: IFI16 induces inflammation in hepatitis B virus-associated glomerulonephritis by regulating the Caspase-1/ IL-1 ß pathway.
  2. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer.
    Title: IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer.
  3. The Role of Inflammasome Activation in Early HIV Infection.
    Title: The Role of Inflammasome Activation in Early HIV Infection.
  4. Increased expression of IFI16 predicts adverse prognosis in multiple myeloma.
    Title: Increased expression of IFI16 predicts adverse prognosis in multiple myeloma.
  5. The role of cyclic GMP-AMP synthase and Interferon-I-inducible protein 16 as candidatebiomarkers of systemic lupus erythematosus.
    Title: The role of cyclic GMP-AMP synthase and Interferon-I-inducible protein 16 as candidatebiomarkers of systemic lupus erythematosus.
  6. IFI16 Isoforms with Cytoplasmic and Nuclear Locations Play Differential Roles in Recognizing Invaded DNA Viruses.
    Title: IFI16 Isoforms with Cytoplasmic and Nuclear Locations Play Differential Roles in Recognizing Invaded DNA Viruses.
  7. IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.
    Title: IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.
  8. The intracellular DNA sensors cGAS and IFI16 do not mediate effective antiviral immune responses to HSV-1 in human microglial cells.
    Title: The intracellular DNA sensors cGAS and IFI16 do not mediate effective antiviral immune responses to HSV-1 in human microglial cells.
  9. Function and Regulation of Nuclear DNA Sensors During Viral Infection and Tumorigenesis.
    Title: Function and Regulation of Nuclear DNA Sensors During Viral Infection and Tumorigenesis.
  10. Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.
    Title: Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.
Submit: New GeneRIF Correction See all GeneRIFs (132)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association and population genetic analysis of C-reactive protein in African American and Hispanic American women.
EBI GWAS Catalog
GWAS of blood cell traits identifies novel associated loci and epistatic interactions in Caucasian and African-American children.
EBI GWAS Catalog
Meta-analysis of genome-wide association studies in celiac disease and rheumatoid arthritis identifies fourteen non-HLA shared loci.
EBI GWAS Catalog
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Nef nef Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Nef may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed
Pr55(Gag) gag HIV-1 Gag interacts with IfI16 as demonstrated by proximity dependent biotinylation proteomics PubMed
Tat tat Microarray analysis indicates HIV-1 Tat upregulates the interferon-responsive gene expression of many proteins, including IFI16, in immature dendritic cells, an effect that likely facilitates the expansion of HIV-1 infection PubMed
Vpr vpr Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Vpr may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed
Vpu vpu Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Vpu may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC9466

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA binding HDA PubMed 
enables double-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables double-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription factor binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in activation of cysteine-type endopeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in activation of innate immune response IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in activation of innate immune response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in autophagy IEA
Inferred from Electronic Annotation
more info
  
involved_in cellular response to glucose starvation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cellular response to interferon-beta IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in cellular response to ionizing radiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in defense response to virus IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in defense response to virus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in inflammatory response IEA
Inferred from Electronic Annotation
more info
  
involved_in innate immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in intrinsic apoptotic signaling pathway by p53 class mediator IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in monocyte differentiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in myeloid cell differentiation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of cysteine-type endopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of innate immune response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of transcription, DNA-templated IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of viral genome replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of cytokine production TAS
Traceable Author Statement
more info
 PubMed 
involved_in positive regulation of interleukin-1 beta production IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in positive regulation of interleukin-1 beta production IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of autophagy IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in regulation of gene expression, epigenetic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
  
colocalizes_with cytosol IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in membrane HDA PubMed 
located_in nuclear speck IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
gamma-interferon-inducible protein 16
Names
interferon-gamma induced protein IFI 16
interferon-inducible myeloid differentiation transcriptional activator

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_029141.2 RefSeqGene

    Range
    14938..60182
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001206567.2 → NP_001193496.1  gamma-interferon-inducible protein 16 isoform 1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) lacks an in-frame exon in the 5' coding region and has an additional in-frame exon in the 3' coding region, compared to variant 2. The additional exon is described as exon 7a in PMID:9718316. The resulting isoform (1) is of the same size but lacks a segment in the N-terminal region and has an additional segment in the C-terminal region, compared to isoform 2. Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.
    Source sequence(s)
    AK296228, BM806453, DA945454, DB273519, M63838
    Consensus CDS
    CCDS58039.1
    UniProtKB/Swiss-Prot
    Q16666
    Related
    ENSP00000352740.3, ENST00000359709.7
    Conserved Domains (3) summary
    cd08305
    Location:10 → 83
    Pyrin; Pyrin: a protein-protein interaction domain
    pfam02760
    Location:145 → 313
    HIN; HIN-200/IF120x domain
    pfam06583
    Location:343 → 545
    Neogenin_C; Neogenin C-terminus

  2. NM_001364867.2 → NP_001351796.1  gamma-interferon-inducible protein 16 isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) encodes the longest isoform (3, also known as IFI 16A).
    Source sequence(s)
    AL359753
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

  3. NM_001376587.1 → NP_001363516.1  gamma-interferon-inducible protein 16 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Related
    ENSP00000295809.7, ENST00000295809.12
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

  4. NM_001376588.1 → NP_001363517.1  gamma-interferon-inducible protein 16 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

  5. NM_001376589.1 → NP_001363518.1  gamma-interferon-inducible protein 16 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

  6. NM_001376591.1 → NP_001363520.1  gamma-interferon-inducible protein 16 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

  7. NM_001376592.1 → NP_001363521.1  gamma-interferon-inducible protein 16 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Related
    ENSP00000404325.2, ENST00000448393.6
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

  8. NM_005531.3 → NP_005522.2  gamma-interferon-inducible protein 16 isoform 2

    See identical proteins and their annotated locations for NP_005522.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) consists of 11 exons and encodes isoform 2 (also known as isoform B, PMIDs:9718316 and 12588705). Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.
    Source sequence(s)
    AL359753, BC017059, DA990524, M63838
    Consensus CDS
    CCDS1180.3
    UniProtKB/Swiss-Prot
    Q16666
    Related
    ENSP00000357113.4, ENST00000368131.8
    Conserved Domains (3) summary
    PTZ00449
    Location:100 → 215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:10 → 83
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201 → 369
    HIN; HIN-200/IF120x domain

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    158999976..159055151
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047419411.1 → XP_047275367.1  gamma-interferon-inducible protein 16 isoform X2

  2. XM_047419406.1 → XP_047275362.1  gamma-interferon-inducible protein 16 isoform X1

  3. XM_047419399.1 → XP_047275355.1  gamma-interferon-inducible protein 16 isoform X1

  4. XM_047419413.1 → XP_047275369.1  gamma-interferon-inducible protein 16 isoform X2

RNA

  1. XR_007059344.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    158137044..158192189
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q16666.3 GenPept UniProtKB/Swiss-Prot:Q16666

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