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IARS1 isoleucyl-tRNA synthetase 1 [ Homo sapiens (human) ]

Gene ID: 3376, updated on 16-Jul-2019

Summary

Official Symbol
IARS1provided by HGNC
Official Full Name
isoleucyl-tRNA synthetase 1provided by HGNC
Primary source
HGNC:HGNC:5330
See related
Ensembl:ENSG00000196305 MIM:600709
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
IRS; IARS; ILRS; ILERS; GRIDHH; PRO0785
Summary
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]
Expression
Ubiquitous expression in thyroid (RPKM 50.6), appendix (RPKM 20.9) and 25 other tissues See more
Orthologs

Genomic context

See IARS1 in Genome Data Viewer
Location:
9q22.31
Exon count:
34
Annotation release Status Assembly Chr Location
109.20190607 current GRCh38.p13 (GCF_000001405.39) 9 NC_000009.12 (92210207..92293756, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (94972489..95056038, complement)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene BEN domain containing 3 pseudogene 2 Neighboring gene uncharacterized LOC112268048 Neighboring gene serine protease 47 Neighboring gene microRNA 3651 Neighboring gene small nucleolar RNA, H/ACA box 84 Neighboring gene chromosome 16 open reading frame 70 pseudogene Neighboring gene nucleolar protein 8 Neighboring gene centromere protein P

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify isoleucyl-tRNA synthetase (IARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify isoleucyl-tRNA synthetase (IARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify isoleucyl-tRNA synthetase (IARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify isoleucyl-tRNA synthetase (IARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
matrix gag HIV-1 MA is identified to have a physical interaction with isoleucyl-tRNA synthetase (IARS) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

  • Homologs of the IARS1 gene: The IARS1 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and frog.
  • Orthologs from Annotation Pipeline: 269 organisms have orthologs with human gene IARS1

Clone Names

  • FLJ20736, FLJ41915, FLJ45144

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
GTPase binding IPI
Inferred from Physical Interaction
more info
PubMed 
aminoacyl-tRNA editing activity IEA
Inferred from Electronic Annotation
more info
 
isoleucine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
isoleucine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
PubMed 
isoleucine-tRNA ligase activity TAS
Traceable Author Statement
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
tRNA binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
aminoacyl-tRNA metabolism involved in translational fidelity IEA
Inferred from Electronic Annotation
more info
 
isoleucyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
isoleucyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
PubMed 
osteoblast differentiation HDA PubMed 
tRNA aminoacylation for protein translation TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
aminoacyl-tRNA synthetase multienzyme complex IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
extracellular exosome HDA PubMed 
membrane HDA PubMed 

General protein information

Preferred Names
isoleucine--tRNA ligase, cytoplasmic
Names
isoleucine tRNA ligase 1, cytoplasmic
NP_002152.2
NP_038203.2

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_051498.1 RefSeqGene

    Range
    5001..88550
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_002161.5NP_002152.2  isoleucine--tRNA ligase, cytoplasmic

    See identical proteins and their annotated locations for NP_002152.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) and variant 2 encode the functional protein.
    Source sequence(s)
    AA731323, BC008318, BC065552, DA335066
    Consensus CDS
    CCDS6694.1
    UniProtKB/Swiss-Prot
    P41252
    UniProtKB/TrEMBL
    Q6P0M4, Q7L4K8
    Related
    ENSP00000406448.3, ENST00000443024.6
    Conserved Domains (3) summary
    cd07961
    Location:640833
    Anticodon_Ia_Ile_ABEc; Anticodon-binding domain of archaeal, bacterial, and eukaryotic cytoplasmic isoleucyl tRNA synthetases
    cd00818
    Location:39640
    IleRS_core; catalytic core domain of isoleucyl-tRNA synthetases
    PLN02882
    Location:61071
    PLN02882; aminoacyl-tRNA ligase
  2. NM_013417.3NP_038203.2  isoleucine--tRNA ligase, cytoplasmic

    See identical proteins and their annotated locations for NP_038203.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AA731323, D28473, DA335066
    Consensus CDS
    CCDS6694.1
    UniProtKB/Swiss-Prot
    P41252
    Related
    ENSP00000364794.3, ENST00000375643.7
    Conserved Domains (3) summary
    cd07961
    Location:640833
    Anticodon_Ia_Ile_ABEc; Anticodon-binding domain of archaeal, bacterial, and eukaryotic cytoplasmic isoleucyl tRNA synthetases
    cd00818
    Location:39640
    IleRS_core; catalytic core domain of isoleucyl-tRNA synthetases
    PLN02882
    Location:61071
    PLN02882; aminoacyl-tRNA ligase

RNA

  1. NR_073446.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AA731323, BX538109, D28473, DA335066
    Related
    ENST00000447699.6

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p13 Primary Assembly

    Range
    92210207..92293756 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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