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DNAJB1 DnaJ heat shock protein family (Hsp40) member B1 [ Homo sapiens (human) ]

Gene ID: 3337, updated on 10-Oct-2024

Summary

Official Symbol
DNAJB1provided by HGNC
Official Full Name
DnaJ heat shock protein family (Hsp40) member B1provided by HGNC
Primary source
HGNC:HGNC:5270
See related
Ensembl:ENSG00000132002 MIM:604572; AllianceGenome:HGNC:5270
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Summary
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression
Ubiquitous expression in esophagus (RPKM 87.8), adrenal (RPKM 84.6) and 24 other tissues See more
Orthologs
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Genomic context

See DNAJB1 in Genome Data Viewer
Location:
19p13.12
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (14514769..14560391, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (14641477..14655999, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (14625581..14640083, complement)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14586151-14586819 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:14587236-14587718 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14589159-14589674 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14590760-14591354 Neighboring gene prostaglandin E receptor 1 Neighboring gene GIPC PDZ domain containing family member 1 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14606254-14607180 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10255 Neighboring gene small nuclear ribonucleoprotein polypeptide G pseudogene 15 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14608461-14608960 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10256 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10257 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr19:14630503-14631232 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:14640150-14640734 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14172 Neighboring gene microRNA 639 Neighboring gene trans-2,3-enoyl-CoA reductase Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14662493-14662992 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14666446-14667327 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:14667328-14668208 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14673655-14674462 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10260 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:14676079-14676885 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14173 Neighboring gene NADH:ubiquinone oxidoreductase subunit B7 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14174 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:14693526-14693669 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14175 Neighboring gene RNA, 7SL, cytoplasmic 337, pseudogene Neighboring gene C-type lectin domain containing 17A

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Nef nef HIV-1 Subtype C Nef upregulates the expression of Hsp40 in HIV-1 infected CEM cells and enhances the increased translocation of Hsp40 into the nucleus PubMed
nef HIV-1 subtype C Nef interacts with Hsp40 in immunoprecipitation and immunofluorescence staining experiments PubMed
nef HIV-1 Nef interacts with Hsp40, leading to increased Hsp40 translocation to the nucleus of infected cells and facilitation of viral gene expression by complex formation of Hsp40 with the cdk9-associated transcription complex PubMed
Tat tat HIV-1 Tat specifically associates with DNAJB1 promoter to upregulate DNAJB1 expression in T cells PubMed
capsid gag HSP40B1 (DNAJB1) inhibits HIV-1 production as measured by the level of viral p24 capsid in the culture supernatant PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATPase activator activity IDA
Inferred from Direct Assay
more info
PubMed 
enables ATPase activator activity TAS
Traceable Author Statement
more info
 
enables ATPase binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables Hsp70 protein binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables Hsp70 protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables cadherin binding HDA PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein folding chaperone IEA
Inferred from Electronic Annotation
more info
 
enables protein-folding chaperone binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables transcription corepressor activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables transcription corepressor activity IDA
Inferred from Direct Assay
more info
PubMed 
enables transcription regulator inhibitor activity IDA
Inferred from Direct Assay
more info
PubMed 
enables unfolded protein binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables unfolded protein binding IDA
Inferred from Direct Assay
more info
PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
located_in dendritic spine IEA
Inferred from Electronic Annotation
more info
 
located_in extracellular exosome HDA PubMed 
located_in glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
located_in neuronal cell body IEA
Inferred from Electronic Annotation
more info
 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 
located_in nucleoplasm TAS
Traceable Author Statement
more info
 
located_in nucleus HDA PubMed 
located_in postsynaptic density IEA
Inferred from Electronic Annotation
more info
 
located_in sperm head IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
dnaJ homolog subfamily B member 1
Names
DnaJ (Hsp40) homolog, subfamily B, member 1
dnaJ protein homolog 1
heat shock 40 kDa protein 1
human DnaJ protein 1
radial spoke 16 homolog B

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001300914.2NP_001287843.1  dnaJ homolog subfamily B member 1 isoform 2

    See identical proteins and their annotated locations for NP_001287843.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).
    Source sequence(s)
    AC012318, BC013157, D49547, DA377896
    Consensus CDS
    CCDS74295.1
    UniProtKB/TrEMBL
    A0A7I2V3K7
    Related
    ENSP00000471073.2, ENST00000598235.2
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  2. NM_001313964.2NP_001300893.1  dnaJ homolog subfamily B member 1 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains alternate 5' terminal exons, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).
    Source sequence(s)
    AC009004, AC012318
    Consensus CDS
    CCDS74295.1
    UniProtKB/TrEMBL
    A0A7I2V3K7
    Related
    ENSP00000444212.1, ENST00000396969.8
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  3. NM_006145.3NP_006136.1  dnaJ homolog subfamily B member 1 isoform 1

    See identical proteins and their annotated locations for NP_006136.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
    Source sequence(s)
    BC013157, D49547, DB454482
    Consensus CDS
    CCDS12312.1
    UniProtKB/Swiss-Prot
    B4DX52, P25685
    UniProtKB/TrEMBL
    Q6FHS4, Q6FI51
    Related
    ENSP00000254322.1, ENST00000254322.3
    Conserved Domains (1) summary
    COG0484
    Location:1340
    DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    14514769..14560391 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047438745.1XP_047294701.1  dnaJ homolog subfamily B member 1 isoform X1

    Related
    ENSP00000504527.1, ENST00000679223.1
  2. XM_006722733.3XP_006722796.1  dnaJ homolog subfamily B member 1 isoform X1

    See identical proteins and their annotated locations for XP_006722796.1

    UniProtKB/TrEMBL
    A0A7I2V3K7
    Related
    ENSP00000471798.2, ENST00000601533.6
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  3. XM_011527956.4XP_011526258.1  dnaJ homolog subfamily B member 1 isoform X1

    See identical proteins and their annotated locations for XP_011526258.1

    UniProtKB/TrEMBL
    A0A7I2V3K7
    Related
    ENSP00000472886.2, ENST00000598692.2
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  4. XM_047438746.1XP_047294702.1  dnaJ homolog subfamily B member 1 isoform X1

    Related
    ENSP00000470063.2, ENST00000596853.6

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    14641477..14655999 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054320814.1XP_054176789.1  dnaJ homolog subfamily B member 1 isoform X1