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DNAJB1 DnaJ heat shock protein family (Hsp40) member B1 [ Homo sapiens (human) ]

Gene ID: 3337, updated on 13-Feb-2019

Summary

Official Symbol
DNAJB1provided by HGNC
Official Full Name
DnaJ heat shock protein family (Hsp40) member B1provided by HGNC
Primary source
HGNC:HGNC:5270
See related
Ensembl:ENSG00000132002 MIM:604572
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Summary
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression
Ubiquitous expression in esophagus (RPKM 87.8), adrenal (RPKM 84.6) and 24 other tissues See more
Orthologs

Genomic context

See DNAJB1 in Genome Data Viewer
Location:
19p13.12
Exon count:
7
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 19 NC_000019.10 (14514764..14530597, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (14625581..14640087, complement)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene prostaglandin E receptor 1 Neighboring gene GIPC PDZ domain containing family member 1 Neighboring gene small nuclear ribonucleoprotein polypeptide G pseudogene 15 Neighboring gene microRNA 639 Neighboring gene trans-2,3-enoyl-CoA reductase Neighboring gene uncharacterized LOC112268253 Neighboring gene NADH:ubiquinone oxidoreductase subunit B7

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Nef nef HIV-1 Subtype C Nef upregulates the expression of Hsp40 in HIV-1 infected CEM cells and enhances the increased translocation of Hsp40 into the nucleus PubMed
nef HIV-1 subtype C Nef interacts with Hsp40 in immunoprecipitation and immunofluorescence staining experiments PubMed
nef HIV-1 Nef interacts with Hsp40, leading to increased Hsp40 translocation to the nucleus of infected cells and facilitation of viral gene expression by complex formation of Hsp40 with the cdk9-associated transcription complex PubMed
Tat tat HIV-1 Tat specifically associates with DNAJB1 promoter to upregulate DNAJB1 expression in T cells PubMed
capsid gag HSP40B1 (DNAJB1) inhibits HIV-1 production as measured by the level of viral p24 capsid in the culture supernatant PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Attenuation phase, organism-specific biosystem (from REACTOME)
    Attenuation phase, organism-specific biosystemAttenuation of the heat shock transcriptional response occurs during continuous exposure to intermediate heat shock conditions or upon recovery from stress (Abravaya et al. 1991). The attenuation pha...
  • Cellular response to heat stress, organism-specific biosystem (from REACTOME)
    Cellular response to heat stress, organism-specific biosystemIn response to exposure to elevated temperature and certain other proteotoxic stimuli (e.g., hypoxia, free radicals) cells activate a number of cytoprotective mechanisms known collectively as "heat s...
  • Cellular responses to stress, organism-specific biosystem (from REACTOME)
    Cellular responses to stress, organism-specific biosystemCells are subject to external molecular and physical stresses such as foreign molecules that perturb metabolic or signaling processes, and changes in temperature or pH. The ability of cells and tissu...
  • HSF1-dependent transactivation, organism-specific biosystem (from REACTOME)
    HSF1-dependent transactivation, organism-specific biosystemAcquisition of DNA binding activity by HSF1 is necessary but insufficient for transcriptional activation (Cotto JJ et al. 1996; Trinklein ND et al. 2004). In addition to having a sequence-specific DN...
  • Influenza A, organism-specific biosystem (from KEGG)
    Influenza A, organism-specific biosystemInfluenza is a contagious respiratory disease caused by influenza virus infection. Influenza A virus is responsible for both annual seasonal epidemics and periodic worldwide pandemics. Novel strains ...
  • Influenza A, conserved biosystem (from KEGG)
    Influenza A, conserved biosystemInfluenza is a contagious respiratory disease caused by influenza virus infection. Influenza A virus is responsible for both annual seasonal epidemics and periodic worldwide pandemics. Novel strains ...
  • MAPK family signaling cascades, organism-specific biosystem (from REACTOME)
    MAPK family signaling cascades, organism-specific biosystemThe mitogen activated protein kinases (MAPKs) are a family of conserved protein serine threonine kinases that respond to varied extracellular stimuli to activate intracellular processes including gen...
  • MAPK6/MAPK4 signaling, organism-specific biosystem (from REACTOME)
    MAPK6/MAPK4 signaling, organism-specific biosystemMAPK6 and MAPK4 (also known as ERK3 and ERK4) are vertebrate-specific atypical MAP kinases. Atypical MAPK are less well characterized than their conventional counterparts, and are generally classifi...
  • NRF2 pathway, organism-specific biosystem (from WikiPathways)
    NRF2 pathway, organism-specific biosystemNRF2 is part of a group of transcription factors called nuclear receptors. It is activated under oxidative stress conditions and subsequently activates several antioxidative genes and proteins.
  • Protein processing in endoplasmic reticulum, organism-specific biosystem (from KEGG)
    Protein processing in endoplasmic reticulum, organism-specific biosystemThe endoplasmic reticulum (ER) is a subcellular organelle where proteins are folded with the help of lumenal chaperones. Newly synthesized peptides enter the ER via the sec61 pore and are glycosylate...
  • Protein processing in endoplasmic reticulum, conserved biosystem (from KEGG)
    Protein processing in endoplasmic reticulum, conserved biosystemThe endoplasmic reticulum (ER) is a subcellular organelle where proteins are folded with the help of lumenal chaperones. Newly synthesized peptides enter the ER via the sec61 pore and are glycosylate...
  • Regulation of HSF1-mediated heat shock response, organism-specific biosystem (from REACTOME)
    Regulation of HSF1-mediated heat shock response, organism-specific biosystemThe ability of HSF1 to respond to cellular stresses is under negative regulation by chaperones, modulation of nucleocytoplasmic shuttling, post-translational modifications and transition from monomer...
  • Signal Transduction, organism-specific biosystem (from REACTOME)
    Signal Transduction, organism-specific biosystemSignal transduction is a process in which extracellular signals elicit changes in cell state and activity. Transmembrane receptors sense changes in the cellular environment by binding ligands, such a...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATPase activator activity IDA
Inferred from Direct Assay
more info
PubMed 
ATPase binding IPI
Inferred from Physical Interaction
more info
PubMed 
Hsp70 protein binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Hsp70 protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
cadherin binding HDA PubMed 
chaperone binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
chaperone binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
transcription corepressor activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
transcription corepressor activity IDA
Inferred from Direct Assay
more info
PubMed 
unfolded protein binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
unfolded protein binding IDA
Inferred from Direct Assay
more info
PubMed 
Component Evidence Code Pubs
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
dendritic spine IEA
Inferred from Electronic Annotation
more info
 
extracellular exosome HDA PubMed 
glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
neuronal cell body IEA
Inferred from Electronic Annotation
more info
 
nucleolus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus HDA PubMed 
nucleus IDA
Inferred from Direct Assay
more info
 
postsynaptic density IEA
Inferred from Electronic Annotation
more info
 
sperm head IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
dnaJ homolog subfamily B member 1
Names
DnaJ (Hsp40) homolog, subfamily B, member 1
dnaJ protein homolog 1
heat shock 40 kDa protein 1
human DnaJ protein 1
radial spoke 16 homolog B

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001300914.1NP_001287843.1  dnaJ homolog subfamily B member 1 isoform 2

    See identical proteins and their annotated locations for NP_001287843.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).
    Source sequence(s)
    BC013157, BM795601, D49547, DA377896
    Consensus CDS
    CCDS74295.1
    UniProtKB/Swiss-Prot
    P25685
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  2. NM_001313964.1NP_001300893.1  dnaJ homolog subfamily B member 1 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains alternate 5' terminal exons, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).
    Source sequence(s)
    AC009004, AC012318
    Consensus CDS
    CCDS74295.1
    UniProtKB/Swiss-Prot
    P25685
    Related
    ENSP00000444212.1, ENST00000396969.8
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  3. NM_006145.3NP_006136.1  dnaJ homolog subfamily B member 1 isoform 1

    See identical proteins and their annotated locations for NP_006136.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
    Source sequence(s)
    BC013157, D49547, DB454482
    Consensus CDS
    CCDS12312.1
    UniProtKB/Swiss-Prot
    P25685
    UniProtKB/TrEMBL
    Q6FHS4
    Related
    ENSP00000254322.1, ENST00000254322.2
    Conserved Domains (1) summary
    COG0484
    Location:1340
    DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p12 Primary Assembly

    Range
    14514764..14530597 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006722734.4XP_006722797.1  dnaJ homolog subfamily B member 1 isoform X1

    See identical proteins and their annotated locations for XP_006722797.1

    UniProtKB/Swiss-Prot
    P25685
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  2. XM_006722733.2XP_006722796.1  dnaJ homolog subfamily B member 1 isoform X1

    See identical proteins and their annotated locations for XP_006722796.1

    UniProtKB/Swiss-Prot
    P25685
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
  3. XM_011527956.3XP_011526258.1  dnaJ homolog subfamily B member 1 isoform X1

    See identical proteins and their annotated locations for XP_011526258.1

    UniProtKB/Swiss-Prot
    P25685
    Conserved Domains (1) summary
    cd10747
    Location:62226
    DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
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