PRMT1 protein arginine methyltransferase 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: PRMT1provided by HGNC
Official Full Name: protein arginine methyltransferase 1provided by HGNC
Primary source: HGNC:HGNC:5187
See related: Ensembl:ENSG00000126457 MIM:602950; Vega:OTTHUMG00000167568
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: ANM1; HCP1; IR1B4; HRMT1L2
Summary: This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
Expression: Ubiquitous expression in ovary (RPKM 42.8), heart (RPKM 38.0) and 25 other tissues See more
Orthologs: mouse all

Genomic context

Location:: 19q13.33

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
109	current	GRCh38.p12 (GCF_000001405.38)	19	NC_000019.10 (49676166..49688450)
105	previous assembly	GRCh37.p13 (GCF_000001405.25)	19	NC_000019.9 (50180409..50191707)

Chromosome 19 - NC_000019.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Study characterizes a novel splicing isoform of PRMT1 that correlates with cellular malignancy. This isoform lacks the dimerization arm of the enzyme, and is catalytically inactive, but able to bind substrates, and blocks cells in the G1 phase of the cell cycle.
Title: A novel splicing isoform of protein arginine methyltransferase 1 (PRMT1) that lacks the dimerization arm and correlates with cellular malignancy.
the abnormal stable complex of FUS-R521C/PRMT1/Nd1-L mRNA could contribute to neurodegeneration upon oxidative stress.
Title: Sequestration of PRMT1 and Nd1-L mRNA into ALS-linked FUS mutant R521C-positive aggregates contributes to neurite degeneration upon oxidative stress.
Important roles of PRMT1 in progression of gastric cancer. Given the dual functions of PRMT1, it is as a potential drug target of gastric cancer with extreme caution.
Title: Protein arginine methyltransferase 1 coordinates the epithelial-mesenchymal transition/proliferation dichotomy in gastric cancer cells.
Mitochondrial Ca(2+) uptake is controlled by protein arginine methyl transferase 1 that asymmetrically methylates MICU1, resulting in decreased Ca(2+) sensitivity. UCP2/3 normalize Ca(2+) sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca(2+) uptake activity.
Title: PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca(2+) uptake in immortalized cells.
High-PRMT1 expression is associated with resistance to cetuximab in triple-negative breast cancer.
Title: The role of PRMT1 in EGFR methylation and signaling in MDA-MB-468 triple-negative breast cancer cells.
a significant role for PRMT1 in HCC progression and metastasis in vitro and in vivo via STAT3 signalling pathway activation. PRMT1 may be a potential novel prognostic biomarker and new therapeutic target for hepatocellular carcinoma.
Title: PRMT1 Promoted HCC Growth and Metastasis In Vitro and In Vivo via Activating the STAT3 Signalling Pathway.
High PRMT1 expression is associated with head and neck and oral cancer.
Title: PRMT1 expression is elevated in head and neck cancer and inhibition of protein arginine methylation by adenosine dialdehyde or PRMT1 knockdown downregulates proliferation and migration of oral cancer cells.
TRIM48 Promotes ASK1 Activation and Cell Death through Ubiquitination-Dependent Degradation of the ASK1-Negative Regulator PRMT1
Title: TRIM48 Promotes ASK1 Activation and Cell Death through Ubiquitination-Dependent Degradation of the ASK1-Negative Regulator PRMT1.
data collectively suggest that silencing of PRMT1 exerts anti-catabolic and anti-inflammatory effects on IL-1beta-induced chondrocytes via suppressing the Gli-1 mediated Hedgehog signaling pathway, indicating that PRMT1 plays a critical role in OA development and serves as a promising therapeutic target for OA.
Title: Knockdown of PRMT1 suppresses IL-1β-induced cartilage degradation and inflammatory responses in human chondrocytes through Gli1-mediated Hedgehog signaling pathway.
Deacetylation on both K200 and K205 by Sirtuin 1 (Sirt1) and acetylation of p300 (EP300) on K205 collaboratively prepare the motif for SCF(Fbxl17) binding thereby triggering PRMT1 protein degradation.
Title: Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCF<sup>Fbxl17</sup>-recognized acetyldegron.

Submit: New GeneRIF Correction See all GeneRIFs (122)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Pr55(Gag)	gag	HIV-1 Gag is identified to have a physical interaction with protein arginine methyltransferase 1 (PRMT1; ANM1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses	PubMed
nucleocapsid	gag	HIV-1 NC is identified to have a physical interaction with protein arginine methyltransferase 1 (PRMT1; ANM1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses	PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

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Chromatin modifying enzymes, organism-specific biosystem (from REACTOME)
Chromatin modifying enzymes, organism-specific biosystemEukaryotic DNA is associated with histone proteins and organized into a complex nucleoprotein structure called chromatin. This structure decreases the accessibility of DNA but also helps to protect i...
Chromatin organization, organism-specific biosystem (from REACTOME)
Chromatin organization, organism-specific biosystemChromatin organization refers to the composition and conformation of complexes between DNA, protein and RNA. It is determined by processes that result in the specification, formation or maintenance o...
Direct p53 effectors, organism-specific biosystem (from Pathway Interaction Database)
Direct p53 effectors, organism-specific biosystem
Direct p53 effectors
FoxO signaling pathway, organism-specific biosystem (from KEGG)
FoxO signaling pathway, organism-specific biosystemThe forkhead box O (FOXO) family of transcription factors regulates the expression of genes in cellular physiological events including apoptosis, cell-cycle control, glucose metabolism, oxidative str...
Gene Expression, organism-specific biosystem (from REACTOME)
Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
Generic Transcription Pathway, organism-specific biosystem (from REACTOME)
Generic Transcription Pathway, organism-specific biosystemOVERVIEW OF TRANSCRIPTION REGULATION: Detailed studies of gene transcription regulation in a wide variety of eukaryotic systems has revealed the general principles and mechanisms by which cell- or t...
Glucagon signaling pathway, organism-specific biosystem (from KEGG)
Glucagon signaling pathway, organism-specific biosystemGlucagon is conventionally regarded as a counterregulatory hormone for insulin and plays a critical anti-hypoglycemic role by maintaining glucose homeostasis in both animals and humans. To increase ...
Glucagon signaling pathway, conserved biosystem (from KEGG)
Glucagon signaling pathway, conserved biosystemGlucagon is conventionally regarded as a counterregulatory hormone for insulin and plays a critical anti-hypoglycemic role by maintaining glucose homeostasis in both animals and humans. To increase ...
Interferon type I signaling pathways, organism-specific biosystem (from WikiPathways)
Interferon type I signaling pathways, organism-specific biosystemThe type I IFNs (IFN? family, IFN?, IFN?, IFN? and IFN?) all bind to and signal via the type I IFN receptor complex. The type I receptor consists of two main IFNAR1, IFNAR2c receptor chains and other...
RMTs methylate histone arginines, organism-specific biosystem (from REACTOME)
RMTs methylate histone arginines, organism-specific biosystemArginine methylation is a common post-translational modification; around 2% of arginine residues are methylated in rat liver nuclei (Boffa et al. 1977). Arginine can be methylated in 3 different ways...
TP53 Regulates Transcription of Cell Cycle Genes, organism-specific biosystem (from REACTOME)
TP53 Regulates Transcription of Cell Cycle Genes, organism-specific biosystemUnder a variety of stress conditions, TP53 (p53), stabilized by stress-induced phosphorylation at least on S15 and S20 serine residues, can induce the transcription of genes involved in cell cycle ar...
TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest, organism-specific biosystem (from REACTOME)
TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest, organism-specific biosystemTP53 contributes to the establishment of G2 arrest by inducing transcription of GADD45A and SFN, and by inhibiting transcription of CDC25C. TP53 induces GADD45A transcription in cooperation with chro...
Transcriptional Regulation by TP53, organism-specific biosystem (from REACTOME)
Transcriptional Regulation by TP53, organism-specific biosystemThe tumor suppressor TP53 (encoded by the gene p53) is a transcription factor. Under stress conditions, it recognizes specific responsive DNA elements and thus regulates the transcription of many gen...
Tryptophan metabolism, organism-specific biosystem (from WikiPathways)
Tryptophan metabolism, organism-specific biosystem
Tryptophan metabolism
mRNA Processing, organism-specific biosystem (from WikiPathways)
mRNA Processing, organism-specific biosystemThis process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' po...

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11S2921 (e-PCR)
- UniSTS:152074

RH91411 (e-PCR)
- UniSTS:84537

RH68301 (e-PCR)
- UniSTS:26699

Hrmt1l2 (e-PCR), detects polymorphism
- UniSTS:275867

STS-N90422 (e-PCR)
- UniSTS:26294

RH65905 (e-PCR)
- UniSTS:2775

NoName (e-PCR)
- UniSTS:486645

Homology

Homologs of the PRMT1 gene: The PRMT1 gene is conserved in Rhesus monkey, dog, cow, mouse, rat, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
Orthologs from Annotation Pipeline: 168 organisms have orthologs with human gene PRMT1
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
N-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
N-methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
RNA binding	HDA more info	PubMed
S-adenosyl-L-methionine binding	IDA Inferred from Direct Assay more info	PubMed
enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
histone methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
histone methyltransferase activity (H4-R3 specific)	IDA Inferred from Direct Assay more info	PubMed
histone methyltransferase activity (H4-R3 specific)	IMP Inferred from Mutant Phenotype more info	PubMed
histone-arginine N-methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
identical protein binding	IDA Inferred from Direct Assay more info	PubMed
identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
methyl-CpG binding	IDA Inferred from Direct Assay more info	PubMed
methyltransferase activity	TAS Traceable Author Statement more info	PubMed
mitogen-activated protein kinase p38 binding	IPI Inferred from Physical Interaction more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
protein methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
protein-arginine N-methyltransferase activity	EXP Inferred from Experiment more info	PubMed
protein-arginine N-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
protein-arginine N-methyltransferase activity	TAS Traceable Author Statement more info
protein-arginine omega-N asymmetric methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
protein-arginine omega-N asymmetric methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
protein-arginine omega-N asymmetric methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
protein-arginine omega-N monomethyltransferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest	TAS Traceable Author Statement more info
cell surface receptor signaling pathway	TAS Traceable Author Statement more info	PubMed
histone H4-R3 methylation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
histone H4-R3 methylation	IDA Inferred from Direct Assay more info	PubMed
histone arginine methylation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
histone methylation	IDA Inferred from Direct Assay more info	PubMed
in utero embryonic development	IEA Inferred from Electronic Annotation more info
negative regulation of megakaryocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
peptidyl-arginine methylation	IDA Inferred from Direct Assay more info	PubMed
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine	IBA Inferred from Biological aspect of Ancestor more info	PubMed
positive regulation of cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of erythrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of hemoglobin biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
positive regulation of p38MAPK cascade	ISS Inferred from Sequence or Structural Similarity more info
protein homooligomerization	IDA Inferred from Direct Assay more info	PubMed
protein methylation	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of megakaryocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
regulation of megakaryocyte differentiation	TAS Traceable Author Statement more info
regulation of transcription, DNA-templated	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytosol	IBA Inferred from Biological aspect of Ancestor more info	PubMed
methylosome	IDA Inferred from Direct Assay more info	PubMed
nucleoplasm	TAS Traceable Author Statement more info
nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein arginine N-methyltransferase 1

Names: HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2; histone-arginine N-methyltransferase PRMT1; interferon receptor 1-bound protein 4

NP_001193971.1

EC 2.1.1.319

NP_001527.3

EC 2.1.1.319

NP_938074.2

EC 2.1.1.319

XP_005258899.1

EC 2.1.1.319

XP_016882223.1

EC 2.1.1.319

XP_016882224.1

EC 2.1.1.319

XP_016882225.1

EC 2.1.1.319

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_031846.1 RefSeqGene

Range

5001..16299

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001207042.2 → NP_001193971.1 protein arginine N-methyltransferase 1 isoform 4

See identical proteins and their annotated locations for NP_001193971.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks three exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.

Source sequence(s)

AI193116, BX352789, DC421815

Consensus CDS

CCDS74425.1

UniProtKB/TrEMBL

A0A087X1W2

Related

ENSP00000484505.1, ENST00000610806.4

Conserved Domains (1) summary

cd02440
Location:74 → 143

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
NM_001536.5 → NP_001527.3 protein arginine N-methyltransferase 1 isoform 1

See identical proteins and their annotated locations for NP_001527.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AI193116, AK304660, DC421815, Y10806

Consensus CDS

CCDS46145.1

UniProtKB/Swiss-Prot

Q99873

UniProtKB/TrEMBL

H7C2I1

Related

ENSP00000406162.2, OTTHUMP00000236178, ENST00000454376.6, OTTHUMT00000395070

Conserved Domains (1) summary

cd02440
Location:92 → 192

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
NM_198318.4 → NP_938074.2 protein arginine N-methyltransferase 1 isoform 3

See identical proteins and their annotated locations for NP_938074.2

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

Source sequence(s)

AI193116, DC421815, Y10807

Consensus CDS

CCDS42592.1

UniProtKB/Swiss-Prot

Q99873

Related

ENSP00000375724.4, OTTHUMP00000236176, ENST00000391851.8, OTTHUMT00000395065

Conserved Domains (1) summary

cd02440
Location:74 → 174

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...

RNA

NR_033397.4 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains an alternate internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AI193116, CN264743, CR407608, DC421815

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

NC_000019.10 Reference GRCh38.p12 Primary Assembly

Range

49676166..49688450

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017026736.1 → XP_016882225.1 protein arginine N-methyltransferase 1 isoform X2

Related

ENSP00000433556.1, OTTHUMP00000236172, ENST00000532489.5, OTTHUMT00000395061

Conserved Domains (1) summary

cd02440
Location:63 → 163

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
XM_017026735.1 → XP_016882224.1 protein arginine N-methyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

Q99873

Conserved Domains (1) summary

cd02440
Location:68 → 168

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
XM_017026734.1 → XP_016882223.1 protein arginine N-methyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

Q99873

Conserved Domains (1) summary

cd02440
Location:68 → 168

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
XM_005258842.1 → XP_005258899.1 protein arginine N-methyltransferase 1 isoform X2

See identical proteins and their annotated locations for XP_005258899.1

Conserved Domains (1) summary

cd02440
Location:63 → 163

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_198319.2: Suppressed sequence

Description

NM_198319.2: This RefSeq was permanently suppressed because it contains a uORF at nt 43..240 that is predicted to inhibit translation of the annotated CDS.