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NARF nuclear prelamin A recognition factor [ Homo sapiens (human) ]

Gene ID: 26502, updated on 24-Nov-2020

Summary

Official Symbol
NARFprovided by HGNC
Official Full Name
nuclear prelamin A recognition factorprovided by HGNC
Primary source
HGNC:HGNC:29916
See related
Ensembl:ENSG00000141562 MIM:605349
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
IOP2
Summary
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in bone marrow (RPKM 13.2), testis (RPKM 12.0) and 25 other tissues See more
Orthologs

Genomic context

See NARF in Genome Data Viewer
Location:
17q25.3
Exon count:
16
Annotation release Status Assembly Chr Location
109.20201120 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (82458198..82490537)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (80415611..80446143)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene HEXD intronic transcript 1 Neighboring gene hexosaminidase D Neighboring gene cytochrome b-245 chaperone 1 Neighboring gene NARF antisense RNA 2 Neighboring gene tRNA-iMet (anticodon CAT) 1-8 Neighboring gene forkhead box K2 Neighboring gene ADP ribosylation factor like GTPase 2 binding protein pseudogene 9

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
retropepsin gag-pol In both human and mouse fibroblasts, HIV-1 protease inhibitors inhibit ZMPSTE24, resulting in a significant accumulation of prelamin A, suggesting the interaction between HIV-1 PR and prelamin A PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ10067, DKFZp434G0420

Gene Ontology Provided by GOA

Function Evidence Code Pubs
lamin binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
lamin binding IPI
Inferred from Physical Interaction
more info
PubMed 
Component Evidence Code Pubs
lamin filament IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
lamin filament IDA
Inferred from Direct Assay
more info
PubMed 
nuclear lamina TAS
Traceable Author Statement
more info
PubMed 
nuclear lumen IDA
Inferred from Direct Assay
more info
PubMed 
nucleolus IDA
Inferred from Direct Assay
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 

General protein information

Preferred Names
nuclear prelamin A recognition factor
Names
iron-only hydrogenase-like protein 2
prenyl-dependent prelamin A binding protein

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001038618.3NP_001033707.1  nuclear prelamin A recognition factor isoform c

    See identical proteins and their annotated locations for NP_001033707.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains a distinct 5' end exon compared to transcript variant 1, resulting in translation initiation from an in-frame downstream AUG, and an isoform (c) with a shorter N-terminus compared to isoform a.
    Source sequence(s)
    AA568571, AC124287, AF128406, AK056966
    Consensus CDS
    CCDS42404.1
    UniProtKB/Swiss-Prot
    Q9UHQ1
    Related
    ENSP00000374656.4, ENST00000390006.8
    Conserved Domains (2) summary
    smart00902
    Location:339387
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:33325
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  2. NM_001083608.2NP_001077077.1  nuclear prelamin A recognition factor isoform d

    See identical proteins and their annotated locations for NP_001077077.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) is missing an in-frame coding exon compared to transcript variant 1, resulting in an isoform (d) that is lacking a 48 aa protein segment compared to isoform a.
    Source sequence(s)
    AC124287, AF128406, BC016440, DB123829
    Consensus CDS
    CCDS42403.1
    UniProtKB/Swiss-Prot
    Q9UHQ1
    Related
    ENSP00000283996.9, ENST00000345415.11
    Conserved Domains (2) summary
    smart00902
    Location:350398
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:44336
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  3. NM_012336.4NP_036468.1  nuclear prelamin A recognition factor isoform a

    See identical proteins and their annotated locations for NP_036468.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the predominant isoform (a).
    Source sequence(s)
    AC124287, AF128406, DB123829
    Consensus CDS
    CCDS32777.1
    UniProtKB/Swiss-Prot
    Q9UHQ1
    Related
    ENSP00000309899.10, ENST00000309794.16
    Conserved Domains (3) summary
    smart00902
    Location:398446
    Fe_hyd_SSU; Iron hydrogenase small subunit
    COG4624
    Location:34445
    Nar1; Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion]
    pfam02906
    Location:92384
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  4. NM_031968.2NP_114174.1  nuclear prelamin A recognition factor isoform b

    See identical proteins and their annotated locations for NP_114174.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an additional in-frame coding exon created by exonization of Alu sequence, and elimination of a premature stop codon by RNA editing (PMID:17326827), compared to transcript variant 1. This results in an isoform (b) that contains a 46 aa protein segment that is missing from isoform a.
    Source sequence(s)
    AF128406, BC000438, DB123829
    UniProtKB/Swiss-Prot
    Q9UHQ1
    Related
    ENSP00000409710.3, ENST00000412079.6
    Conserved Domains (3) summary
    smart00902
    Location:444492
    Fe_hyd_SSU; Iron hydrogenase small subunit
    COG4624
    Location:34491
    Nar1; Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion]
    pfam02906
    Location:92430
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20201120

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

    Range
    82458198..82490537
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017024450.2XP_016879939.1  nuclear prelamin A recognition factor isoform X4

    Conserved Domains (2) summary
    smart00902
    Location:198246
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:1184
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  2. XM_017024449.2XP_016879938.1  nuclear prelamin A recognition factor isoform X3

    Conserved Domains (2) summary
    smart00902
    Location:227275
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:43213
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  3. XM_005256340.4XP_005256397.1  nuclear prelamin A recognition factor isoform X2

    See identical proteins and their annotated locations for XP_005256397.1

    UniProtKB/Swiss-Prot
    Q9UHQ1
    Conserved Domains (2) summary
    smart00902
    Location:339387
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:33325
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  4. XM_011523544.2XP_011521846.1  nuclear prelamin A recognition factor isoform X5

    Conserved Domains (1) summary
    pfam02906
    Location:92217
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  5. XM_006722278.4XP_006722341.1  nuclear prelamin A recognition factor isoform X1

    Conserved Domains (3) summary
    smart00902
    Location:353401
    Fe_hyd_SSU; Iron hydrogenase small subunit
    COG4624
    Location:34400
    Nar1; Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion]
    pfam02906
    Location:92339
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  6. XM_011523543.3XP_011521845.1  nuclear prelamin A recognition factor isoform X4

    Conserved Domains (2) summary
    smart00902
    Location:198246
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:1184
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
  7. XM_011523541.2XP_011521843.1  nuclear prelamin A recognition factor isoform X2

    See identical proteins and their annotated locations for XP_011521843.1

    UniProtKB/Swiss-Prot
    Q9UHQ1
    Conserved Domains (2) summary
    smart00902
    Location:339387
    Fe_hyd_SSU; Iron hydrogenase small subunit
    pfam02906
    Location:33325
    Fe_hyd_lg_C; Iron only hydrogenase large subunit, C-terminal domain
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