ZNF281 zinc finger protein 281 [Homo sapiens (human)] - Gene

Summary

Official Symbol: ZNF281provided by HGNC
Official Full Name: zinc finger protein 281provided by HGNC
Primary source: HGNC:HGNC:13075
See related: Ensembl:ENSG00000162702 MIM:618703; AllianceGenome:HGNC:13075
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GZP1; ZBP99; ZBP-99; ZNP-99
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of gene expression; negative regulation of transcription by RNA polymerase II; and positive regulation of DNA-templated transcription. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Expression: Ubiquitous expression in bone marrow (RPKM 10.1), appendix (RPKM 5.7) and 25 other tissues See more
Orthologs: all
: Try the new Gene page
Try the new Transcripts and proteins table

Genomic context

Location:: 1q32.1

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2025_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (200404951..200410036, complement)
RS_2025_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (199661900..199740523, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (200374079..200379164, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Tissue-specific circular RNA induction during human fetal development
Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
BioProject: PRJNA270632
Publication: PMID 26076956
Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cancer-associated fibroblasts facilitate premetastatic niche formation through lncRNA SNHG5-mediated angiogenesis and vascular permeability in breast cancer.
Title: Cancer-associated fibroblasts facilitate premetastatic niche formation through lncRNA SNHG5-mediated angiogenesis and vascular permeability in breast cancer.
ZNF281 Promotes Colon Fibroblast Activation in TGFbeta1-Induced Gut Fibrosis.
Title: ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis.
Cervical carcinoma progression is aggravated by lncRNA ZNF281 by binding KLF15.
Title: Cervical carcinoma progression is aggravated by lncRNA ZNF281 by binding KLF15.
ZNF281/Zfp281 is a target of miR-1 and counteracts muscle differentiation.
Title: ZNF281/Zfp281 is a target of miR-1 and counteracts muscle differentiation.
ZNF281 is recruited on DNA breaks to facilitate DNA repair by non-homologous end joining.
Title: ZNF281 is recruited on DNA breaks to facilitate DNA repair by non-homologous end joining.
LncRNA-ZNF281 Interacts with miR-539 to Promote Hepatocellular Carcinoma Cell Invasion and Migration.
Title: LncRNA-ZNF281 Interacts with miR-539 to Promote Hepatocellular Carcinoma Cell Invasion and Migration.
Analyses indicated that circAGFG1 acts as a sponge for miR-203 to repress the effect of miR-203 on its target, ZNF281. In conclusion, study suggests that circAGFG1 promotes NSCLC growth and metastasis though a circAGFG1/miR-203/ZNF281 axis.
Title: CircAGFG1 sponges miR-203 to promote EMT and metastasis of non-small-cell lung cancer by upregulating ZNF281 expression.
Data indicate transcription factor ZNF281 as a player of intestinal inflammation and fibrosis, and in the pathogenetic mechanisms underlying inflammatory bowel disease.
Title: Transcription Factor ZNF281: A Novel Player in Intestinal Inflammation and Fibrosis.
ZNF281 mRNA and protein levels were significantly increased in colorectal cancer (CRC) tissues compared with normal colon tissues, and high ZNF281 expression was associated with advanced T stage, N stage, TNM stage and differentiation. Therefore, ZNF281 expression might be an independent prognostic indicator in CRC patients.
Title: ZNF281 Regulates Cell Proliferation, Migration and Invasion in Colorectal Cancer through Wnt/β-Catenin Signaling.
These observations suggest that ZNF281 is a controller of neuronal differentiation that should be evaluated as a prognostic marker in neuroblastoma.
Title: ZNF281 inhibits neuronal differentiation and is a prognostic marker for neuroblastoma.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

RH47383 (e-PCR)
- UniSTS:54827

RH71424 (e-PCR)
- UniSTS:9777

A007J21 (e-PCR)
- UniSTS:20532

ZNF281__4806 (e-PCR)
- UniSTS:463355

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISS Inferred from Sequence or Structural Similarity more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in embryonic body morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in embryonic body morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in stem cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in stem cell differentiation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: zinc finger protein 281

Names: GC-box-binding zinc finger protein 1; ZNP-99 transcription factor; transcription factor ZBP-99; zinc finger DNA-binding protein 99

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001281293.2 → NP_001268222.1 zinc finger protein 281 isoform 1

See identical proteins and their annotated locations for NP_001268222.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).

Source sequence(s)

AC104461, AJ132592, BP216938

Consensus CDS

CCDS1402.1

UniProtKB/Swiss-Prot

A6NF48, B3KMX2, Q5RKW5, Q9NY92, Q9Y2X9

Related

ENSP00000356322.1, ENST00000367353.2

Conserved Domains (2) summary

sd00017
Location:263 → 283

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:331 → 356

zf-H2C2_2; Zinc-finger double domain
NM_001281294.2 → NP_001268223.1 zinc finger protein 281 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is missing an internal segment compared to isoform 1.

Source sequence(s)

AC104461, AK022921, AK094701, BC051905, BG611294, DB150929

Consensus CDS

CCDS60384.1

UniProtKB/Swiss-Prot

Q9Y2X9

UniProtKB/TrEMBL

B3KMX4

Related

ENSP00000356321.3, ENST00000367352.3

Conserved Domains (3) summary

sd00017
Location:227 → 247

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:225 → 247

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:295 → 320

zf-H2C2_2; Zinc-finger double domain
NM_012482.5 → NP_036614.1 zinc finger protein 281 isoform 1

See identical proteins and their annotated locations for NP_036614.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein (isoform 1).

Source sequence(s)

AC104461, BC060820

Consensus CDS

CCDS1402.1

UniProtKB/Swiss-Prot

A6NF48, B3KMX2, Q5RKW5, Q9NY92, Q9Y2X9

Related

ENSP00000294740.2, ENST00000294740.3

Conserved Domains (2) summary

sd00017
Location:263 → 283

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:331 → 356

zf-H2C2_2; Zinc-finger double domain