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BRD4 bromodomain containing 4 [ Homo sapiens (human) ]

Gene ID: 23476, updated on 11-Nov-2018

Summary

Official Symbol
BRD4provided by HGNC
Official Full Name
bromodomain containing 4provided by HGNC
Primary source
HGNC:HGNC:13575
See related
Ensembl:ENSG00000141867 MIM:608749; Vega:OTTHUMG00000183252
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CAP; MCAP; HUNK1; HUNKI
Summary
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in placenta (RPKM 10.8), testis (RPKM 9.2) and 25 other tissues See more
Orthologs

Genomic context

See BRD4 in Genome Data Viewer
Location:
19p13.12
Exon count:
23
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 19 NC_000019.10 (15236836..15332543, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (15348301..15443354, complement)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene notch 3 Neighboring gene microRNA 6795 Neighboring gene epoxide hydrolase 3 Neighboring gene A-kinase anchoring protein 8 Neighboring gene A-kinase anchoring protein 8 like

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 replication is inhibited by BRD4; siRNA-mediated knockdown allows for Tat-induced latency reversal PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat The RxL or KxL motif and the polybasic cluster of HIV-1 Tat and BRD4 exhibit similar importance for P-TEFb activation PubMed
tat The C-terminal 34 residues (1329-1362) of BRD4 are crucial for interaction with P-TEFb. Overexpression of the BRD4 P-TEFb-interacting domain disrupts the interaction between HIV-1 Tat and P-TEFb PubMed
tat CDK9-CycT1-AFF1 is stimulated by HIV-1 Tat and transferred as a single complex unit to BRD4 upon stress-induced disruption of AFF1-containing 7SK snRNP (HEXIM1, MEPCE, LARP7, 7SK RNA, CDK9, CycT1, and AFF1) PubMed
tat Phosphorylation of CDK9 at position Ser175 regulates the competition between HIV-1 Tat and BRD4 for P-TEFb binding PubMed
tat Brd4 inhibits HIV-1 Tat-human super elongation complex (components AFF4, ELL2, CycT1, and CDK9) by competing with HIV-1 Tat for binding to P-TEFb on HIV-1 promoter PubMed
tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
RNA polymerase II C-terminal domain binding IDA
Inferred from Direct Assay
more info
PubMed 
RNA polymerase II CTD heptapeptide repeat kinase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
lysine-acetylated histone binding IDA
Inferred from Direct Assay
more info
PubMed 
p53 binding IDA
Inferred from Direct Assay
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Component Evidence Code Pubs
condensed nuclear chromosome IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
bromodomain-containing protein 4
Names
chromosome-associated protein
NP_001317313.1
NP_055114.1
NP_490597.1
XP_011526156.1
XP_011526158.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001330384.1NP_001317313.1  bromodomain-containing protein 4 isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long.
    Source sequence(s)
    AC005776, BC035266
    Consensus CDS
    CCDS82307.1
    UniProtKB/Swiss-Prot
    O60885
    UniProtKB/TrEMBL
    A0A024R7H8
    Related
    ENSP00000353112.4, ENST00000360016.9
    Conserved Domains (3) summary
    cd05497
    Location:58164
    Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    cd05498
    Location:353454
    Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    pfam17035
    Location:609673
    BET; Bromodomain extra-terminal - transcription regulation
  2. NM_014299.2NP_055114.1  bromodomain-containing protein 4 isoform short

    See identical proteins and their annotated locations for NP_055114.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long.
    Source sequence(s)
    AC004798, BC035266, Y12059
    Consensus CDS
    CCDS46004.1
    UniProtKB/Swiss-Prot
    O60885
    Related
    ENSP00000360901.3, OTTHUMP00000271251, ENST00000371835.8, OTTHUMT00000465801
    Conserved Domains (3) summary
    cd05497
    Location:58164
    Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    cd05498
    Location:353454
    Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    pfam17035
    Location:609673
    BET; Bromodomain extra-terminal - transcription regulation
  3. NM_058243.2NP_490597.1  bromodomain-containing protein 4 isoform long

    See identical proteins and their annotated locations for NP_490597.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (long) encodes the full length isoform.
    Source sequence(s)
    AF386649, BC035266
    Consensus CDS
    CCDS12328.1
    UniProtKB/Swiss-Prot
    O60885
    Related
    ENSP00000263377.1, OTTHUMP00000271250, ENST00000263377.6, OTTHUMT00000465800
    Conserved Domains (6) summary
    cd05497
    Location:58164
    Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    cd05498
    Location:353454
    Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    pfam17035
    Location:609673
    BET; Bromodomain extra-terminal - transcription regulation
    pfam17105
    Location:13211362
    BRD4_CDT; C-terminal domain of bromodomain protein 4
    cl25742
    Location:12301271
    ERM; Ezrin/radixin/moesin family
    cl27169
    Location:809922
    ZipA; ZipA C-terminal domain. ZipA, a membrane-anchored protein, is one of at least nine essential gene products necessary for assembly of the septal ring which mediates cell division in E.coli. ZipA and FtsA directly bind FtsZ, a homolog of eukaryotic ...

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p12 Primary Assembly

    Range
    15236836..15332543 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011527854.2XP_011526156.1  bromodomain-containing protein 4 isoform X1

    See identical proteins and their annotated locations for XP_011526156.1

    UniProtKB/Swiss-Prot
    O60885
    Conserved Domains (6) summary
    cd05497
    Location:58164
    Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    cd05498
    Location:353454
    Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    pfam17035
    Location:609673
    BET; Bromodomain extra-terminal - transcription regulation
    pfam17105
    Location:13211362
    BRD4_CDT; C-terminal domain of bromodomain protein 4
    cl25742
    Location:12301271
    ERM; Ezrin/radixin/moesin family
    cl27169
    Location:809922
    ZipA; ZipA C-terminal domain. ZipA, a membrane-anchored protein, is one of at least nine essential gene products necessary for assembly of the septal ring which mediates cell division in E.coli. ZipA and FtsA directly bind FtsZ, a homolog of eukaryotic ...
  2. XM_011527856.3XP_011526158.1  bromodomain-containing protein 4 isoform X2

    See identical proteins and their annotated locations for XP_011526158.1

    UniProtKB/Swiss-Prot
    O60885
    Conserved Domains (3) summary
    cd05497
    Location:58164
    Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    cd05498
    Location:353454
    Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
    pfam17035
    Location:609673
    BET; Bromodomain extra-terminal - transcription regulation
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