P2rx3 purinergic receptor P2X, ligand-gated ion channel, 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: P2rx3provided by MGI
Official Full Name: purinergic receptor P2X, ligand-gated ion channel, 3provided by MGI
Primary source: MGI:MGI:1097160
See related: Ensembl:ENSMUSG00000027071 AllianceGenome:MGI:1097160
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: P2X3; 4930513E20Rik
Summary: Enables extracellularly ATP-gated monoatomic cation channel activity. Involved in modulation of chemical synaptic transmission. Acts upstream of or within several processes, including neuromuscular synaptic transmission; response to ATP; and smooth muscle contraction. Located in axon. Is active in Schaffer collateral - CA1 synapse and hippocampal mossy fiber to CA3 synapse. Is expressed in several structures, including branchial arch; embryo ectoderm; gut; nervous system; and retina. Orthologous to human P2RX3 (purinergic receptor P2X 3). [provided by Alliance of Genome Resources, Apr 2025]
Expression: Biased expression in testis adult (RPKM 11.8), CNS E14 (RPKM 1.5) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 D; 2 49.45 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (84826893..84867446, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (84996549..85037108, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Finely ordered intracellular domain harbors an allosteric site to modulate physiopathological function of P2X3 receptors.
Title: Finely ordered intracellular domain harbors an allosteric site to modulate physiopathological function of P2X3 receptors.
The purinergic receptors 2X3 on spiral ganglion neurons enhance the medial olivocochlear reflex in mice after long-term moderate noise exposure.
Title: The purinergic receptors 2X3 on spiral ganglion neurons enhance the medial olivocochlear reflex in mice after long-term moderate noise exposure.
Involvement of P2X2 receptor in the medial prefrontal cortex in ATP modulation of the passive coping response to behavioral challenge.
Title: Involvement of P2X2 receptor in the medial prefrontal cortex in ATP modulation of the passive coping response to behavioral challenge.
The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development.
Title: The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development.
Functional Coupling of Slack Channels and P2X3 Receptors Contributes to Neuropathic Pain Processing.
Title: Functional Coupling of Slack Channels and P2X3 Receptors Contributes to Neuropathic Pain Processing.
This study demonstrated that the P2X3-immunoreactive nerve endings in the rat tracheal mucosa have unique morphological characteristics, and these endings may be rapidly adapting receptors and/or irritant receptors that are activated by mucosal irritant stimuli.
Title: Morphology of P2X3-immunoreactive nerve endings in the rat tracheal mucosa.
Data suggest that the negative inhibition of P2X3R activity by the BNP/NPR-A pathway results in a decreased P2X3R-mediated excitability of trigeminal neurons in wildetype cultures. In familial hemiplegic migraine type-1 model cultures, however, lack of efficient P2X3Rs downregulation contributes to the neuronal hyperexcitability phenotype.
Title: Loss of inhibition by brain natriuretic peptide over P2X3 receptors contributes to enhanced spike firing of trigeminal ganglion neurons in a mouse model of familial hemiplegic migraine type-1.
P2X3 receptors on mouse trigeminal ganglion neurons are subjected to contrasting modulation by inhibitory brain natriuretic peptide and facilitatory calcitonin gene-related peptide that both operate via complex intracellular signaling.
Title: Inefficient constitutive inhibition of P2X3 receptors by brain natriuretic peptide system contributes to sensitization of trigeminal sensory neurons in a genetic mouse model of familial hemiplegic migraine.
mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments
Title: Brain natriuretic peptide constitutively downregulates P2X3 receptors by controlling their phosphorylation state and membrane localization.
may be involved in skeletal pain due to high bone turnover
Title: Acid-sensing ion channel 3 or P2X2/3 is involved in the pain-like behavior under a high bone turnover state in ovariectomized mice.

Submit: New GeneRIF Correction See all GeneRIFs (46)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

05.MMHAP4FRB8.seq (e-PCR)
- UniSTS:122815

P2rx3 (e-PCR), detects polymorphism
- UniSTS:499186

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables extracellularly ATP-gated monoatomic cation channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables extracellularly ATP-gated monoatomic cation channel activity	IGI Inferred from Genetic Interaction more info	PubMed
enables extracellularly ATP-gated monoatomic cation channel activity	ISO Inferred from Sequence Orthology more info
enables extracellularly ATP-gated monoatomic cation channel activity	ISS Inferred from Sequence or Structural Similarity more info
enables extracellularly ATP-gated monoatomic cation channel activity	TAS Traceable Author Statement more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables purinergic nucleotide receptor activity	ISO Inferred from Sequence Orthology more info
enables purinergic nucleotide receptor activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in behavioral response to formalin induced pain	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within behavioral response to pain	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within behavioral response to pain	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in calcium ion transmembrane transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to ATP	ISO Inferred from Sequence Orthology more info
involved_in cellular response to ATP	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
involved_in inorganic cation transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in inorganic cation transmembrane transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within monoatomic cation transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within monoatomic ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuronal action potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within peristalsis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein homotrimerization	ISO Inferred from Sequence Orthology more info
involved_in protein homotrimerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in purinergic nucleotide receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to ATP	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to ATP	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to carbohydrate	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to cold	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to mechanical stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to temperature stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sensory perception of taste	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within urinary bladder smooth muscle contraction	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within urinary bladder smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
is_active_in hippocampal mossy fiber to CA3 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in hippocampal mossy fiber to CA3 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
part_of receptor complex	ISO Inferred from Sequence Orthology more info	PubMed
located_in terminal bouton	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: P2X purinoceptor 3

Names: ATP receptor; purinergic receptor P2X3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145526.3 → NP_663501.2 P2X purinoceptor 3

See identical proteins and their annotated locations for NP_663501.2

Status: VALIDATED

Source sequence(s)

AL935159

Consensus CDS

CCDS16200.1

UniProtKB/Swiss-Prot

A2AW02, Q3UR32, Q8R1U4

UniProtKB/TrEMBL

A2AW04

Related

ENSMUSP00000028465.8, ENSMUST00000028465.14

Conserved Domains (1) summary

pfam00864
Location:8 → 363

P2X_receptor; ATP P2X receptor

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

84826893..84867446 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006499210.5 → XP_006499273.1 P2X purinoceptor 3 isoform X1

See identical proteins and their annotated locations for XP_006499273.1

UniProtKB/TrEMBL

A2AW04

Conserved Domains (1) summary

pfam00864
Location:1 → 289

P2X_receptor; ATP P2X receptor
XM_030250185.2 → XP_030106045.1 P2X purinoceptor 3 isoform X1

UniProtKB/TrEMBL

A2AW04

Conserved Domains (1) summary

pfam00864
Location:1 → 289

P2X_receptor; ATP P2X receptor
XM_011239452.3 → XP_011237754.1 P2X purinoceptor 3 isoform X1

See identical proteins and their annotated locations for XP_011237754.1

UniProtKB/TrEMBL

A2AW04

Conserved Domains (1) summary

pfam00864
Location:1 → 289

P2X_receptor; ATP P2X receptor
XM_030250183.2 → XP_030106043.1 P2X purinoceptor 3 isoform X1

UniProtKB/TrEMBL

A2AW04

Conserved Domains (1) summary

pfam00864
Location:1 → 289

P2X_receptor; ATP P2X receptor
XM_030250184.2 → XP_030106044.1 P2X purinoceptor 3 isoform X1

UniProtKB/TrEMBL

A2AW04

Conserved Domains (1) summary

pfam00864
Location:1 → 289

P2X_receptor; ATP P2X receptor
XM_030250186.2 → XP_030106046.1 P2X purinoceptor 3 isoform X1

UniProtKB/TrEMBL

A2AW04

Conserved Domains (1) summary

pfam00864
Location:1 → 289

P2X_receptor; ATP P2X receptor