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Zp2 zona pellucida glycoprotein 2 [ Mus musculus (house mouse) ]

Gene ID: 22787, updated on 25-Apr-2024

Summary

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Official Symbol
Zp2provided by MGI
Official Full Name
zona pellucida glycoprotein 2provided by MGI
Primary source
MGI:MGI:99214
See related
Ensembl:ENSMUSG00000030911 AllianceGenome:MGI:99214
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Zp-2
Summary
This gene encodes a member of the zona pellucida family of glycoproteins that play an important role in the survival of growing oocytes, successful fertilization and the passage of early embryos through the oviduct. The encoded preproprotein undergoes proteolytic processing to generate the mature polypeptide that is incorporated into the extracellular matrix surrounding mouse oocytes. Mice lacking the encoded protein develop defective zonae pellucidae that disrupt folliculogenesis, fertility and development. [provided by RefSeq, Sep 2016]
Expression
Restricted expression toward ovary adult (RPKM 15.6) See more
Orthologs
human all
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Genomic context

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Location:
7 F2; 7 64.38 cM
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (119725985..119744514, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (120126762..120145291, complement)

Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene dynein, axonemal, heavy chain 3 Neighboring gene ribosomal protein S9 pseudogene Neighboring gene STARR-seq mESC enhancer starr_20042 Neighboring gene lipid droplet assembly factor 1 Neighboring gene RIKEN cDNA 4930505K13 gene Neighboring gene STARR-seq mESC enhancer starr_20043 Neighboring gene ankyrin repeat and sterile alpha motif domain containing 4B

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. The bioactivities of the central segment of Zp2 polypeptide. Cao Z, et al. Zygote, 2016 Oct. PMID 27193969
  2. Mouse granulosa cells contribute more to the mRNA synthesis of mZP2 than oocyte does. Xie Y, et al. Cell Biochem Funct, 2010 Dec 2. PMID 20979237
  3. Primary structure and developmental expression of Dp ZP2, a vitelline envelope glycoprotein homolog of mouse ZP2, in Discoglossus pictus, one of the oldest living Anuran species. Vaccaro MC, et al. Mol Reprod Dev, 2001 Jun. PMID 11389548
  4. Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development. Rankin TL, et al. Development, 2001 Apr. PMID 11245577
  5. ZAP-1 DNA binding activity is first detected at the onset of zona pellucida gene expression in embryonic mouse oocytes. Millar SE, et al. Dev Biol, 1993 Aug. PMID 8344459

See all (74) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.
    Title: Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.
  2. The central peptide of ZP2 (190-505) is immunogenic and contains zona pellucida-specific epitopes.
    Title: The bioactivities of the central segment of Zp2 polypeptide.
  3. ZP2(51-149) sperm-binding domain is necessary for mouse gamete recognition and penetration through the zona pellucida.
    Title: A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans.
  4. Studies indicate that eggs from mZP2-/- and mZP3-/- females lack a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts.
    Title: Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility.
  5. Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
    Title: Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
  6. Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease.
    Title: Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
  7. Cytoplasmic tails are necessary to prevent intracellular oligomerization while ensuring incorporation of processed ZP2 and ZP3 into the zona pellucida.
    Title: ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida.
  8. The granulosa cells contribute more to the mZP2 mRNA synthesis than oocyte does.
    Title: Mouse granulosa cells contribute more to the mRNA synthesis of mZP2 than oocyte does.
  9. data indicate that sperm-egg recognition depends on the cleavage status of ZP2 and that binding at the surface of the zona is not sufficient to induce sperm acrosome exocytosis
    Title: Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein.
  10. characterization of native mouse zona pellucida proteins by mass spectrometry
    Title: Structural characterization of native mouse zona pellucida proteins using mass spectrometry.
Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables acrosin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables acrosin binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables structural constituent of egg coat IBA
Inferred from Biological aspect of Ancestor
more info
  
enables structural constituent of egg coat ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in binding of sperm to zona pellucida IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within binding of sperm to zona pellucida IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in binding of sperm to zona pellucida IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in prevention of polyspermy IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in prevention of polyspermy IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within single fertilization IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in collagen-containing extracellular matrix IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in collagen-containing extracellular matrix IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in egg coat IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in egg coat ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in multivesicular body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
zona pellucida sperm-binding protein 2
Names
zona pellucida glycoprotein ZP2
zona pellucida protein A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001374631.1 → NP_001361560.1  zona pellucida sperm-binding protein 2 isoform 2 preproprotein

    Status: REVIEWED

    Source sequence(s)
    AC122853
    Consensus CDS
    CCDS90322.1
    UniProtKB/TrEMBL
    Q3UX44, Q6IR45
    Related
    ENSMUSP00000147097.2, ENSMUST00000208874.2
    Conserved Domains (1) summary
    smart00241
    Location:365 → 630
    ZP; Zona pellucida (ZP) domain

  2. NM_011775.7 → NP_035905.1  zona pellucida sperm-binding protein 2 isoform 1 preproprotein

    See identical proteins and their annotated locations for NP_035905.1

    Status: REVIEWED

    Source sequence(s)
    AK135903, AK138699, DV657934
    Consensus CDS
    CCDS21792.1
    UniProtKB/Swiss-Prot
    P20239
    UniProtKB/TrEMBL
    Q6IR45
    Related
    ENSMUSP00000033207.5, ENSMUST00000033207.6
    Conserved Domains (1) summary
    smart00241
    Location:365 → 630
    ZP; Zona pellucida (ZP) domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000073.7 Reference GRCm39 C57BL/6J

    Range
    119725985..119744514 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006507590.4 → XP_006507653.1  zona pellucida sperm-binding protein 2 isoform X1

    UniProtKB/TrEMBL
    Q6IR45
    Conserved Domains (1) summary
    smart00241
    Location:237 → 502
    ZP; Zona pellucida (ZP) domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P20239.1 GenPept UniProtKB/Swiss-Prot:P20239

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