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Pfkl phosphofructokinase, liver, B-type [ Mus musculus (house mouse) ]

Gene ID: 18641, updated on 1-Mar-2024

Summary

Official Symbol
Pfklprovided by MGI
Official Full Name
phosphofructokinase, liver, B-typeprovided by MGI
Primary source
MGI:MGI:97547
See related
Ensembl:ENSMUSG00000020277 AllianceGenome:MGI:97547
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
PFK-B; PFK-L; ATP-PFK
Summary
Enables 6-phosphofructokinase activity. Acts upstream of or within several processes, including fructose 6-phosphate metabolic process; glycolytic process through fructose-6-phosphate; and negative regulation of insulin secretion. Located in cytosol. Is expressed in several structures, including alimentary system; brain; genitourinary system; musculoskeletal system; and sensory organ. Orthologous to human PFKL (phosphofructokinase, liver type). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in colon adult (RPKM 59.9), ovary adult (RPKM 45.7) and 28 other tissues See more
Orthologs
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Genomic context

See Pfkl in Genome Data Viewer
Location:
10 C1; 10 39.72 cM
Exon count:
22
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (77822781..77845641, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (77986947..78009807, complement)

Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene transient receptor potential cation channel, subfamily M, member 2 Neighboring gene predicted gene, 40705 Neighboring gene STARR-seq mESC enhancer starr_27210 Neighboring gene STARR-positive B cell enhancer ABC_E9033 Neighboring gene cilia and flagella associated protein 410 Neighboring gene STARR-positive B cell enhancer ABC_E5151 Neighboring gene STARR-seq mESC enhancer starr_27212 Neighboring gene STARR-seq mESC enhancer starr_27213 Neighboring gene STARR-seq mESC enhancer starr_27214 Neighboring gene STARR-seq mESC enhancer starr_27215 Neighboring gene autoimmune regulator Neighboring gene DNA methyltransferase 3-like Neighboring gene predicted gene, 51775 Neighboring gene icos ligand

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1) 
  • Endonuclease-mediated (1) 

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 6-phosphofructokinase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables 6-phosphofructokinase activity IDA
Inferred from Direct Assay
more info
PubMed 
contributes_to 6-phosphofructokinase activity ISO
Inferred from Sequence Orthology
more info
 
enables 6-phosphofructokinase activity ISO
Inferred from Sequence Orthology
more info
 
enables AMP binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables ATP binding ISO
Inferred from Sequence Orthology
more info
 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
 
enables fructose binding ISO
Inferred from Sequence Orthology
more info
 
enables fructose-6-phosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables fructose-6-phosphate binding ISO
Inferred from Sequence Orthology
more info
 
enables identical protein binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables kinase binding ISO
Inferred from Sequence Orthology
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables monosaccharide binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables monosaccharide binding ISO
Inferred from Sequence Orthology
more info
 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
involved_in canonical glycolysis IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in fructose 1,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in fructose 1,6-bisphosphate metabolic process ISO
Inferred from Sequence Orthology
more info
 
involved_in fructose 6-phosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within fructose 6-phosphate metabolic process IDA
Inferred from Direct Assay
more info
PubMed 
involved_in fructose 6-phosphate metabolic process ISO
Inferred from Sequence Orthology
more info
 
involved_in glycolytic process ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within glycolytic process through fructose-6-phosphate IDA
Inferred from Direct Assay
more info
PubMed 
involved_in glycolytic process through fructose-6-phosphate IDA
Inferred from Direct Assay
more info
PubMed 
involved_in glycolytic process through fructose-6-phosphate IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within negative regulation of insulin secretion IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within response to glucose IDA
Inferred from Direct Assay
more info
PubMed 
involved_in response to glucose ISO
Inferred from Sequence Orthology
more info
 
Component Evidence Code Pubs
part_of 6-phosphofructokinase complex IBA
Inferred from Biological aspect of Ancestor
more info
 
part_of 6-phosphofructokinase complex ISO
Inferred from Sequence Orthology
more info
 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
 
located_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
 

General protein information

Preferred Names
ATP-dependent 6-phosphofructokinase, liver type
Names
6-phosphofructokinase type B
6-phosphofructokinase, liver type
phosphofructo-1-kinase isozyme B
phosphofructokinase 1
phosphohexokinase
NP_001345722.1
NP_032852.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001358793.1NP_001345722.1  ATP-dependent 6-phosphofructokinase, liver type isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC153507, AK030892, AK081313, BP766623, BY070321, EL605376
    UniProtKB/TrEMBL
    Q8CD98
    Conserved Domains (1) summary
    cl00204
    Location:1458
    PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...
  2. NM_008826.5NP_032852.2  ATP-dependent 6-phosphofructokinase, liver type isoform 1

    See identical proteins and their annotated locations for NP_032852.2

    Status: VALIDATED

    Source sequence(s)
    AK030892, AK081313, BP766623, BY070321
    Consensus CDS
    CCDS35955.1
    UniProtKB/Swiss-Prot
    P12382, Q8VDX7
    Related
    ENSMUSP00000020522.9, ENSMUST00000020522.9
    Conserved Domains (2) summary
    cd00764
    Location:13773
    Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...
    TIGR02478
    Location:16755
    6PF1K_euk; 6-phosphofructokinase, eukaryotic type

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000076.7 Reference GRCm39 C57BL/6J

    Range
    77822781..77845641 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)