Naca nascent polypeptide-associated complex alpha polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nacaprovided by MGI
Official Full Name: nascent polypeptide-associated complex alpha polypeptideprovided by MGI
Primary source: MGI:MGI:106095
See related: Ensembl:ENSMUSG00000061315 AllianceGenome:MGI:106095
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: skNAC; Gm1878; mKIAA0363
Summary: Enables TBP-class protein binding activity and transcription coactivator activity. Involved in several processes, including heart development; negative regulation of striated muscle cell apoptotic process; and positive regulation of skeletal muscle tissue growth. Acts upstream of or within myoblast migration. Located in cytoplasm and nucleus. Is expressed in several structures, including aorta; genitourinary system; gut; liver; and spleen. Orthologous to human NACA (nascent polypeptide associated complex subunit alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in heart adult (RPKM 34.3), CNS E11.5 (RPKM 24.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10; 10 D3

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (127869144..127884506)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (128035346..128048637)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The ribosomal chaperone NACA recruits PHD2 to cotranslationally modify HIF-alpha.
Title: The ribosomal chaperone NACA recruits PHD2 to cotranslationally modify HIF-α.
The data suggest that skNAC and Smyd1 might be major players in the processes of myofibrillogenesis and sarcomerogenesis, probably specifically at later stages.
Title: siRNA-mediated inhibition of skNAC and Smyd1 expression disrupts myofibril organization: Immunofluorescence and electron microscopy study in C2C12 cells.
Data show that reduction of alpha chain of nascent polypeptide associated complex (alphaNAC) levels decreased basal expression of Low density lipoprotein receptor-Related Protein 6 (Lrp6) by 30%.
Title: Lrp6 is a target of the PTH-activated αNAC transcriptional coregulator.
the skNAC-Smyd1 complex is involved in transcriptional regulation both via the control of histone methylation and histone (de)acetylation.
Title: skNAC and Smyd1 in transcriptional control.
skNAC binds to the E3 SUMO ligase mammalian Mms21/Nse2 and that knockdown of Nse2 expression inhibits specific aspects of myogenic differentiation, accompanied by a partial blockade of the nuclear-to-cytoplasmic translocation of the skNAC-Smyd1 complex
Title: The E3 SUMO ligase Nse2 regulates sumoylation and nuclear-to-cytoplasmic translocation of skNAC-Smyd1 in myogenesis.
depletion of alphaNAC in multiple types of cancer cells induce typical apoptotic cell death. This anti-apoptotic function is mediated by the FADD/c-Jun N-terminal kinase pathway.
Title: αNAC inhibition of the FADD-JNK axis plays anti-apoptotic role in multiple cancer cells.
We propose that maximal transcriptional repression by FIAT requires its interaction with SUMOylated, HDAC2-interacting aNAC
Title: SUMOylated αNAC potentiates transcriptional repression by FIAT.
Altered gene dosages for Galphas and alpha- NAC in compound heterozygous mice result in reduced bone mass, increased numbers of osteocytes, and enhanced expression of Sost.
Title: The PTH-Gαs-protein kinase A cascade controls αNAC localization to regulate bone mass.
The bone phenotype of compound Naca(+/-); Fiat(+/-) heterozygotes confirms that FIAT and alphaNAC are part of a common genetic pathway and support a role for the FIAT/alphaNAC interaction in normal bone physiology.
Title: Altered gene dosage confirms the genetic interaction between FIAT and αNAC.
data suggest that skNAC controls myoblast migration and sarcomere architecture in a calpain-dependent manner
Title: skNAC depletion stimulates myoblast migration and perturbs sarcomerogenesis by enhancing calpain 1 and 3 activity.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from Annotation Pipeline: 406 organisms have orthologs with human gene Naca
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables TBP-class protein binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Process	Evidence Code	Pubs
involved_in cardiac ventricle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart trabecula morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myoblast migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein localization to endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of striated muscle cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell proliferation involved in heart morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of skeletal muscle tissue growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to membrane	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of skeletal muscle fiber development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in skeletal muscle tissue regeneration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in wound healing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
part_of nascent polypeptide-associated complex	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: nascent polypeptide-associated complex subunit alpha

Names: alpha-NAC, muscle-specific form; alpha-NAC/1.9.2; nascent polypeptide-associated complex alpha subunit; nascent polypeptide-associated complex subunit alpha, muscle-specific form

NP_001106670.1

EC 5.3.99.3

NP_001269905.1

EC 5.3.99.3

NP_038636.2

EC 5.3.99.3

XP_036011564.1

EC 5.3.99.3

XP_036011565.1

EC 5.3.99.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113199.1 → NP_001106670.1 nascent polypeptide-associated complex subunit alpha isoform a

See identical proteins and their annotated locations for NP_001106670.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (a).

Source sequence(s)

AC131120, CN840834, U48364

Consensus CDS

CCDS48723.1

UniProtKB/Swiss-Prot

E9QMB1, P70670

Related

ENSMUSP00000089680.6, ENSMUST00000092048.13

Conserved Domains (3) summary

PHA03247
Location:1057 → 1559

PHA03247; large tegument protein UL36; Provisional

cd14415
Location:2141 → 2186

UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)

cd22054
Location:2056 → 2103

NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit
NM_001282976.1 → NP_001269905.1 nascent polypeptide-associated complex subunit alpha isoform b

See identical proteins and their annotated locations for NP_001269905.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) has an additional segment in the 5' UTR and lacks an in-frame large exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. Variants 2 and 3 encode the same isoform b.

Source sequence(s)

AC131120, BC083340, CX236470, U22151

Consensus CDS

CCDS24258.1

UniProtKB/Swiss-Prot

Q4FZL8, Q60817

UniProtKB/TrEMBL

A0A6P5QGE5, A0A8C6GKG0

Conserved Domains (2) summary

cd14415
Location:169 → 214

UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)

cd22054
Location:84 → 131

NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit
NM_013608.3 → NP_038636.2 nascent polypeptide-associated complex subunit alpha isoform b

See identical proteins and their annotated locations for NP_038636.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an internal in-frame large exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. Variants 2 and 3 encode the same isoform b.

Source sequence(s)

BC083340, CN840834, U48364

Consensus CDS

CCDS24258.1

UniProtKB/Swiss-Prot

P70670, Q4FZL8, Q60817

UniProtKB/TrEMBL

A0A6P5QGE5, A0A8C6GKG0

Related

ENSMUSP00000073532.8, ENSMUST00000073868.9

Conserved Domains (2) summary

cd14415
Location:169 → 214

UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)

cd22054
Location:84 → 131

NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

127869144..127884506

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155672.1 → XP_036011565.1 nascent polypeptide-associated complex subunit alpha isoform X2

UniProtKB/Swiss-Prot

Q4FZL8

UniProtKB/TrEMBL

A0A6P5QGE5, A0A8C6GKG0

Conserved Domains (2) summary

cd14415
Location:169 → 214

UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)

cd22054
Location:84 → 131

NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit
XM_036155671.1 → XP_036011564.1 nascent polypeptide-associated complex subunit alpha isoform X1

UniProtKB/Swiss-Prot

E9QMB1

Conserved Domains (3) summary

PHA03247
Location:1057 → 1559

PHA03247; large tegument protein UL36; Provisional

cd14415
Location:2141 → 2186

UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)

cd22054
Location:2056 → 2103

NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit