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Gapdh glyceraldehyde-3-phosphate dehydrogenase [ Mus musculus (house mouse) ]

Gene ID: 14433, updated on 8-Jan-2023

Summary

Official Symbol
Gapdhprovided by MGI
Official Full Name
glyceraldehyde-3-phosphate dehydrogenaseprovided by MGI
Primary source
MGI:MGI:95640
See related
Ensembl:ENSMUSG00000057666 AllianceGenome:MGI:95640
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gapd
Summary
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
Annotation information
Note: GeneID 14433 was annotated on chromosome 6 on NCBI's Build 36.1 and GeneID 407972 (then associated with the functional Gapdh) was not annotated. Sequences associated with GeneID 14433 also align to chromosome 2 A3, the location of a highly related Gapdh pseudogene that was not annotated on Build 36.1. To connect the nomenclature with the annotation, GeneID 14433 was merged with GeneID 407972 and it now represents the functional Gapdh gene on chromosome 6. The RefSeq for Gapdh is NM_008084 with NM_001001303.1 being secondary to it. The Gapdh pseudogene on chromosome 2 is now represented by GeneID 100033452 and the RefSeq NG_005903.1. [13 Feb 2013]
Expression
Ubiquitous expression in placenta adult (RPKM 30.4), frontal lobe adult (RPKM 26.7) and 28 other tissues See more
Orthologs
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Genomic context

See Gapdh in Genome Data Viewer
Location:
6 F2; 6 59.32 cM
Exon count:
8
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (125138815..125143430, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (125161852..125166467, complement)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene NOP2 nucleolar protein Neighboring gene intermediate filament family orphan 1 Neighboring gene non-SMC condensin I complex, subunit D2 Neighboring gene small Cajal body-specific RNA 10 Neighboring gene mitochondrial ribosomal protein L51

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC102544, MGC102546, MGC103190, MGC103191, MGC105239

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables NAD binding IEA
Inferred from Electronic Annotation
more info
 
enables NADP binding IEA
Inferred from Electronic Annotation
more info
 
enables aspartic-type endopeptidase inhibitor activity ISO
Inferred from Sequence Orthology
more info
 
enables disordered domain specific binding ISO
Inferred from Sequence Orthology
more info
 
enables enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IDA
Inferred from Direct Assay
more info
PubMed 
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity ISO
Inferred from Sequence Orthology
more info
 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
 
enables microtubule binding ISO
Inferred from Sequence Orthology
more info
 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
 
enables oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
 
enables peptidyl-cysteine S-nitrosylase activity ISO
Inferred from Sequence Orthology
more info
 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide ISO
Inferred from Sequence Orthology
more info
 
involved_in apoptotic process ISO
Inferred from Sequence Orthology
more info
 
involved_in cAMP-mediated signaling ISO
Inferred from Sequence Orthology
more info
 
involved_in canonical glycolysis IDA
Inferred from Direct Assay
more info
PubMed 
involved_in canonical glycolysis IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in carbohydrate metabolic process ISO
Inferred from Sequence Orthology
more info
 
involved_in cellular response to type II interferon ISO
Inferred from Sequence Orthology
more info
 
involved_in defense response to fungus ISO
Inferred from Sequence Orthology
more info
 
involved_in gluconeogenesis IDA
Inferred from Direct Assay
more info
PubMed 
involved_in gluconeogenesis ISO
Inferred from Sequence Orthology
more info
 
involved_in glucose metabolic process IEA
Inferred from Electronic Annotation
more info
 
involved_in glycolytic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in glycolytic process ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within immune system process IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within innate immune response IEA
Inferred from Electronic Annotation
more info
 
involved_in killing by host of symbiont cells ISO
Inferred from Sequence Orthology
more info
 
involved_in killing of cells of another organism ISO
Inferred from Sequence Orthology
more info
 
involved_in microtubule cytoskeleton organization ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within multicellular organism development IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of endopeptidase activity ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of translation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of translation ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of vascular associated smooth muscle cell apoptotic process ISO
Inferred from Sequence Orthology
more info
 
involved_in neuron apoptotic process ISO
Inferred from Sequence Orthology
more info
 
involved_in peptidyl-cysteine S-trans-nitrosylation ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of I-kappaB kinase/NF-kappaB signaling ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of cytokine production ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of type I interferon production ISO
Inferred from Sequence Orthology
more info
 
involved_in protein stabilization ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within regulation of translation IEA
Inferred from Electronic Annotation
more info
 
involved_in response to ammonium ion ISO
Inferred from Sequence Orthology
more info
 
Component Evidence Code Pubs
part_of GAIT complex IDA
Inferred from Direct Assay
more info
PubMed 
part_of GAIT complex ISO
Inferred from Sequence Orthology
more info
 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
is_active_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
is_active_in glutamatergic synapse ISO
Inferred from Sequence Orthology
more info
 
located_in intracellular membrane-bounded organelle ISO
Inferred from Sequence Orthology
more info
 
located_in late endosome lumen TAS
Traceable Author Statement
more info
 
located_in lipid droplet ISO
Inferred from Sequence Orthology
more info
 
located_in microtubule cytoskeleton ISO
Inferred from Sequence Orthology
more info
 
located_in mitochondrion HDA PubMed 
located_in myelin sheath HDA PubMed 
located_in nuclear membrane ISO
Inferred from Sequence Orthology
more info
 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
 
is_active_in postsynaptic density, intracellular component ISO
Inferred from Sequence Orthology
more info
 
part_of ribonucleoprotein complex ISO
Inferred from Sequence Orthology
more info
 

General protein information

Preferred Names
glyceraldehyde-3-phosphate dehydrogenase
Names
peptidyl-cysteine S-nitrosylase GAPDH
NP_001276655.1
NP_032110.1
XP_036021733.1

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001289726.2NP_001276655.1  glyceraldehyde-3-phosphate dehydrogenase isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AC166162
    Consensus CDS
    CCDS80613.1
    UniProtKB/Swiss-Prot
    P16858
    UniProtKB/TrEMBL
    A0A0A0MQF6, D2KHZ9
    Related
    ENSMUSP00000113942.3, ENSMUST00000117757.9
    Conserved Domains (3) summary
    COG0057
    Location:27359
    GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
    pfam00044
    Location:28176
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    pfam02800
    Location:181338
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
  2. NM_001411840.1NP_001398769.1  glyceraldehyde-3-phosphate dehydrogenase isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC166162
    UniProtKB/Swiss-Prot
    Q5U410
    UniProtKB/TrEMBL
    D2KHZ9
  3. NM_001411841.1NP_001398770.1  glyceraldehyde-3-phosphate dehydrogenase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166162
  4. NM_001411842.1NP_001398771.1  glyceraldehyde-3-phosphate dehydrogenase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166162
  5. NM_001411843.1NP_001398772.1  glyceraldehyde-3-phosphate dehydrogenase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166162
  6. NM_001411844.1NP_001398773.1  glyceraldehyde-3-phosphate dehydrogenase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166162
  7. NM_001411845.1NP_001398774.1  glyceraldehyde-3-phosphate dehydrogenase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166162
  8. NM_008084.4NP_032110.1  glyceraldehyde-3-phosphate dehydrogenase isoform 2

    See identical proteins and their annotated locations for NP_032110.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.
    Source sequence(s)
    AC166162
    Consensus CDS
    CCDS51913.1
    UniProtKB/Swiss-Prot
    P16858, Q5U410
    UniProtKB/TrEMBL
    D2KHZ9
    Related
    ENSMUSP00000113213.2, ENSMUST00000118875.8
    Conserved Domains (1) summary
    PLN02272
    Location:2330
    PLN02272; glyceraldehyde-3-phosphate dehydrogenase

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    125138815..125143430 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036165840.1XP_036021733.1  glyceraldehyde-3-phosphate dehydrogenase isoform X1

    Conserved Domains (1) summary
    PLN02272
    Location:2330
    PLN02272; glyceraldehyde-3-phosphate dehydrogenase