Cacybp calcyclin binding protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cacybpprovided by MGI
Official Full Name: calcyclin binding proteinprovided by MGI
Primary source: MGI:MGI:1270839
See related: Ensembl:ENSMUSG00000014226 AllianceGenome:MGI:1270839
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SIP
Summary: Enables protein domain specific binding activity and ubiquitin protein ligase binding activity. Acts upstream of or within cellular response to leukemia inhibitory factor. Located in cytoplasm and nuclear envelope lumen. Part of SCF ubiquitin ligase complex. Is expressed in several structures, including alimentary system; genitourinary system; liver lobe; nervous system; and sensory organ. Orthologous to human CACYBP (calcyclin binding protein). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 42.1), liver E14 (RPKM 32.8) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1; 1 H2.1

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (160029937..160040462, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (160202367..160212892, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

involvement of CacyBP/SIP in the regulation of p38 kinase activity, in addition to that of ERK1/2, might point to the function of CacyBP/SIP in pro-survival and pro-apoptotic pathways.
Title: MAP kinase p38 is a novel target of CacyBP/SIP phosphatase.
In undifferentiated and differentiated tumor cells, CacyBP level has an effect on the ERK1/2-CREB-BDNF pathway.
Title: Distinct effect of CacyBP/SIP on the ERK1/2-CREB-BDNF pathway in undifferentiated and differentiated neuroblastoma NB2a cells.
Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP) was initially described as a binding partner of S100A6 in the Ehrlich ascites tumor cells and later as a Siah-1-interacting protein. Its role has been studied in various mouse tumors and cell lines. Review.
Title: The potential role of CacyBP/SIP in tumorigenesis.
sumoylated CacyBP/SIP is present in the cytoplasmic and not in the nuclear fraction. We have also established that lysine 16 is the residue which undergoes sumoylation in the CacyBP/SIP protein.
Title: The CacyBP/SIP protein is sumoylated in neuroblastoma NB2a cells.
different activity of CacyBP/SIP in neuroblastoma NB2a and colon cancer HCT116 cells might affect the ERK1/2 pathway in the differentiation or proliferation processes
Title: CacyBP/SIP phosphatase activity in neuroblastoma NB2a and colon cancer HCT116 cells.
new insight into the interaction between S100 proteins and CacyBP/SIP
Title: S100A6 protein negatively regulates CacyBP/SIP-mediated inhibition of gastric cancer cell proliferation and tumorigenesis.
SIP (-/-) embryonic fibroblasts have increased levels of cytosolic p27 and exhibit increased cell motility compared to wild-type cells.
Title: Siah1/SIP regulates p27(kip1) stability and cell migration under metabolic stress.
CacyBP/SIP exhibits a phosphatase activity toward ERK1/2 kinases while its E217K mutant does not.
Title: ERK1/2 is dephosphorylated by a novel phosphatase--CacyBP/SIP.
Cacybp is associated with acute lung injury
Title: Haplotype association mapping of acute lung injury in mice implicates activin a receptor, type 1.
Data indicated that CacyBP/SIP could simultaneously interact with tubulin and actin, suggesting that CacyBP/SIP might link actin and tubulin cytoskeletons.
Title: Tubulin binding protein, CacyBP/SIP, induces actin polymerization and may link actin and tubulin cytoskeletons.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (2)
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:234247

RH124492 (e-PCR)
- UniSTS:161314

Homology

Homologs of the Cacybp gene: The Cacybp gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, fruit fly, mosquito, A.thaliana, rice, and frog.
Orthologs from Annotation Pipeline: 492 organisms have orthologs with human gene Cacybp
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables S100 protein binding	IEA Inferred from Electronic Annotation more info
enables molecular adaptor activity	EXP Inferred from Experiment more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables tubulin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cardiac muscle cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in heart development	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in negative regulation of cell death	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA replication	ISO Inferred from Sequence Orthology more info
involved_in response to growth hormone	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of beta-catenin destruction complex	ISO Inferred from Sequence Orthology more info
located_in cell body	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope lumen	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: calcyclin-binding protein

Names: siah-interacting protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009786.2 → NP_033916.1 calcyclin-binding protein

See identical proteins and their annotated locations for NP_033916.1

Status: VALIDATED

Source sequence(s)

AC154873, AK013924

Consensus CDS

CCDS15406.1

UniProtKB/Swiss-Prot

O70140, Q9CXW3

Related

ENSMUSP00000014370.6, ENSMUST00000014370.11

Conserved Domains (3) summary

cd06468
Location:76 → 168

p23_CacyBP; p23_like domain found in proteins similar to Calcyclin-Binding Protein(CacyBP)/Siah-1-interacting protein (SIP). CacyBP/SIP interacts with S100A6 (calcyclin), with some other members of the S100 family, with tubulin, and with Siah-1 and Skp-1. The latter ...

pfam05002
Location:183 → 218

SGS; SGS domain

pfam09032
Location:5 → 76

Siah-Interact_N; Siah interacting protein, N terminal