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Rnf216 ring finger protein 216 [ Mus musculus (house mouse) ]

Gene ID: 108086, updated on 2-Nov-2024

Summary

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Official Symbol
Rnf216provided by MGI
Official Full Name
ring finger protein 216provided by MGI
Primary source
MGI:MGI:1344349
See related
Ensembl:ENSMUSG00000045078 AllianceGenome:MGI:1344349
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
UIP83; TRIAD3; Ubce7ip1; 2810055G22Rik; F830018F18Rik
Summary
Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in several processes, including protein K48-linked ubiquitination; protein catabolic process, modulating synaptic transmission; and regulation of postsynaptic neurotransmitter receptor internalization. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in several cellular components, including Schaffer collateral - CA1 synapse; glutamatergic synapse; and postsynaptic endocytic zone. Human ortholog(s) of this gene implicated in Gordon Holmes syndrome. Orthologous to human RNF216 (ring finger protein 216). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in testis adult (RPKM 22.7), adrenal adult (RPKM 18.3) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Rnf216 in Genome Data Viewer
Location:
5 G2; 5 81.86 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (142976648..143098793, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (142990893..143113041, complement)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E6395 Neighboring gene STARR-positive B cell enhancer ABC_E674 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:143670916-143671255 Neighboring gene predicted gene, 38733 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:143682513-143682666 Neighboring gene actin, beta Neighboring gene STARR-seq mESC enhancer starr_14775 Neighboring gene STARR-seq mESC enhancer starr_14777 Neighboring gene fascin actin-bundling protein 1 Neighboring gene STARR-seq mESC enhancer starr_14779 Neighboring gene VISTA enhancer mm1619 Neighboring gene predicted gene 43380 Neighboring gene STARR-seq mESC enhancer starr_14782 Neighboring gene STARR-seq mESC enhancer starr_14783 Neighboring gene STARR-positive B cell enhancer ABC_E3631 Neighboring gene STARR-seq mESC enhancer starr_14784 Neighboring gene nonconserved acetylation island sequence M61 enhancer Neighboring gene olfactory receptor family 10 subfamily AH member 1, pseudogene 1 Neighboring gene predicted gene, 38734 Neighboring gene STARR-seq mESC enhancer starr_14788 Neighboring gene predicted gene 7291

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Gordon Holmes Syndrome Model Mice Exhibit Alterations in Microglia, Age, and Sex-Specific Disruptions in Cognitive and Proprioceptive Function. George AJ, et al. eNeuro, 2024 Jan. PMID 38164552, Free PMC Article
  2. The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis. George AJ, et al. iScience, 2022 Jun 17. PMID 35620441, Free PMC Article
  3. Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis. Yuan Z, et al. Biomed Pharmacother, 2020 Aug. PMID 32460187
  4. Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9. Lu X, et al. Basic Res Cardiol, 2020 Feb 1. PMID 32008145
  5. Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9 impairs mitochondrial bioenergetics and exacerbates diabetic cardiomyopathy. Kong C, et al. J Adv Res, 2024 Jul. PMID 37625569, Free PMC Article

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9 impairs mitochondrial bioenergetics and exacerbates diabetic cardiomyopathy.
    Title: Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9 impairs mitochondrial bioenergetics and exacerbates diabetic cardiomyopathy.
  2. RNF216 affects the stability of STAU2 in the hypothalamus.
    Title: RNF216 affects the stability of STAU2 in the hypothalamus.
  3. RNF216 regulates meiosis and PKA stability in the testes.
    Title: RNF216 regulates meiosis and PKA stability in the testes.
  4. Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis.
    Title: Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis.
  5. Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9.
    Title: Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9.
  6. An essential function of RNF216 in spermatogenesis and male fertility and suggest a critical role for RNF216 in human gonadal development.
    Title: RNF216 is essential for spermatogenesis and male fertility†.
  7. Triad3a promotes the negative feedback loop independently of typical RipK1 ubiquitin editing enzymes
    Title: Triad3a induces the degradation of early necrosome to limit RipK1-dependent cytokine production and necroptosis.
  8. Low RNF216 expression is associated with obesity-induced inflammation.
    Title: Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Toll-like Receptor 4 Expression via Up-regulation of E3 Ubiquitin-protein Ligase RNF216.
  9. missense mutations in TRIAD3 abolished the interaction of TRIAD3A with Arc, disrupting Arc ubiquitination, and consequently Arc degradation.
    Title: TRIAD3/RNF216 mutations associated with Gordon Holmes syndrome lead to synaptic and cognitive impairments via Arc misregulation.
  10. identifies a novel negative regulator of autophagy and suggests that RNF216 may be a target for treatment of inflammatory diseases
    Title: Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in apoptotic process IEA
Inferred from Electronic Annotation
more info
  
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in protein K48-linked ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K48-linked ubiquitination ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in protein catabolic process, modulating synaptic transmission IEA
Inferred from Electronic Annotation
more info
  
involved_in protein catabolic process, modulating synaptic transmission ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of defense response to virus by host ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of defense response to virus by host ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of interferon-beta production ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of interferon-beta production ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of postsynaptic neurotransmitter receptor internalization IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of postsynaptic neurotransmitter receptor internalization ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in Schaffer collateral - CA1 synapse IEA
Inferred from Electronic Annotation
more info
  
is_active_in Schaffer collateral - CA1 synapse ISO
Inferred from Sequence Orthology
more info
  
located_in clathrin-coated vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
  
is_active_in glutamatergic synapse ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in postsynaptic endocytic zone IEA
Inferred from Electronic Annotation
more info
  
is_active_in postsynaptic endocytic zone ISO
Inferred from Sequence Orthology
more info
  
located_in presynapse IEA
Inferred from Electronic Annotation
more info
  
is_active_in presynapse ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase RNF216
Names
E3 ubiquitin ligase
RING-type E3 ubiquitin transferase RNF216
triad domain-containing protein 3
ubcM4-interacting protein 83
ubiquitin-conjugating enzyme 7-interacting protein 1
NP_001346780.1
NP_542128.2
NP_996993.1
XP_006504707.1
XP_006504708.1
XP_006504709.1
XP_017176080.1
XP_017176081.1
XP_017176082.1
XP_030109902.1
XP_036020615.1
XP_036020616.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001359851.1 → NP_001346780.1  E3 ubiquitin-protein ligase RNF216 isoform C

    Status: VALIDATED

    Description
    Transcript Variant: This variant (C) differs in the 5' UTR and coding sequence compared to variant B. The resulting isoform (C) is shorter at the N-terminus compared to isoform B.
    Source sequence(s)
    AC113281
    Conserved Domains (1) summary
    cd16630
    Location:310 → 366
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

  2. NM_080561.4 → NP_542128.2  E3 ubiquitin-protein ligase RNF216 isoform A

    See identical proteins and their annotated locations for NP_542128.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (A) uses an alternate in-frame splice junction compared to variant B. The resulting isoform (A) has the same N- and C-termini but is shorter compared to isoform B.
    Source sequence(s)
    BB854046, BC065066
    Consensus CDS
    CCDS80455.1
    UniProtKB/Swiss-Prot
    P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3
    Related
    ENSMUSP00000052563.10, ENSMUST00000053498.13
    Conserved Domains (2) summary
    cd16630
    Location:501 → 557
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:643 → 693
    IBR; IBR domain, a half RING-finger domain

  3. NM_207110.1 → NP_996993.1  E3 ubiquitin-protein ligase RNF216 isoform B

    See identical proteins and their annotated locations for NP_996993.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (B) represents the longest transcript and encodes the longest isoform (B).
    Source sequence(s)
    AC113281, AK147861, BB854046, BC065066
    Consensus CDS
    CCDS57400.1
    UniProtKB/Swiss-Prot
    P58283
    Related
    ENSMUSP00000143705.2, ENSMUST00000200607.5
    Conserved Domains (2) summary
    cd16630
    Location:558 → 614
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:700 → 750
    IBR; IBR domain, a half RING-finger domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    142976648..143098793 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030254042.1 → XP_030109902.1  E3 ubiquitin-protein ligase RNF216 isoform X4

    Conserved Domains (2) summary
    cd16630
    Location:416 → 472
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:558 → 608
    IBR; IBR domain, a half RING-finger domain

  2. XM_006504646.4 → XP_006504709.1  E3 ubiquitin-protein ligase RNF216 isoform X3

    Conserved Domains (2) summary
    cd16630
    Location:473 → 529
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:615 → 665
    IBR; IBR domain, a half RING-finger domain

  3. XM_006504644.5 → XP_006504707.1  E3 ubiquitin-protein ligase RNF216 isoform X1

    See identical proteins and their annotated locations for XP_006504707.1

    UniProtKB/Swiss-Prot
    P58283
    Conserved Domains (2) summary
    cd16630
    Location:558 → 614
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:700 → 750
    IBR; IBR domain, a half RING-finger domain

  4. XM_006504645.5 → XP_006504708.1  E3 ubiquitin-protein ligase RNF216 isoform X2

    See identical proteins and their annotated locations for XP_006504708.1

    UniProtKB/Swiss-Prot
    P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3
    Conserved Domains (2) summary
    cd16630
    Location:501 → 557
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:643 → 693
    IBR; IBR domain, a half RING-finger domain

  5. XM_017320593.3 → XP_017176082.1  E3 ubiquitin-protein ligase RNF216 isoform X5

    Conserved Domains (1) summary
    cd16630
    Location:310 → 366
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

  6. XM_017320592.3 → XP_017176081.1  E3 ubiquitin-protein ligase RNF216 isoform X5

    Conserved Domains (1) summary
    cd16630
    Location:310 → 366
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

  7. XM_036164722.1 → XP_036020615.1  E3 ubiquitin-protein ligase RNF216 isoform X1

    Conserved Domains (2) summary
    cd16630
    Location:558 → 614
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:700 → 750
    IBR; IBR domain, a half RING-finger domain

  8. XM_017320591.3 → XP_017176080.1  E3 ubiquitin-protein ligase RNF216 isoform X1

    UniProtKB/Swiss-Prot
    P58283
    Conserved Domains (2) summary
    cd16630
    Location:558 → 614
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:700 → 750
    IBR; IBR domain, a half RING-finger domain

  9. XM_036164723.1 → XP_036020616.1  E3 ubiquitin-protein ligase RNF216 isoform X2

    UniProtKB/Swiss-Prot
    P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3
    Conserved Domains (2) summary
    cd16630
    Location:501 → 557
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
    pfam01485
    Location:643 → 693
    IBR; IBR domain, a half RING-finger domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P58283.3 GenPept UniProtKB/Swiss-Prot:P58283

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