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RNF216 ring finger protein 216 [ Homo sapiens (human) ]

Gene ID: 54476, updated on 12-Aug-2018

Summary

Official Symbol
RNF216provided by HGNC
Official Full Name
ring finger protein 216provided by HGNC
Primary source
HGNC:HGNC:21698
See related
Ensembl:ENSG00000011275 MIM:609948; Vega:OTTHUMG00000155500
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ZIN; CAHH; U7I1; TRIAD3; UBCE7IP1
Summary
This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in testis (RPKM 16.3), lymph node (RPKM 10.2) and 25 other tissues See more
Orthologs

Genomic context

See RNF216 in Genome Data Viewer
Location:
7p22.1
Exon count:
20
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 7 NC_000007.14 (5620041..5781730, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 7 NC_000007.13 (5659672..5821361, complement)

Chromosome 7 - NC_000007.14Genomic Context describing neighboring genes Neighboring gene S100 calcium binding protein A11 pseudogene Neighboring gene uncharacterized LOC107986762 Neighboring gene fascin actin-bundling protein 1 Neighboring gene VISTA enhancer hs1623 Neighboring gene RNF216 intronic transcript 1 Neighboring gene microRNA 6874 Neighboring gene zinc finger protein 815, pseudogene Neighboring gene RNA, 7SL, cytoplasmic 556, pseudogene Neighboring gene oncomodulin

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of ring finger protein 216 (RNF216; TRIAD3) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Vif vif Triad 3/ZIN binds to purified HIV-1 Vif in vitro, interacts with HIV-1 Vif in transfected human 293T cells, and can impair viral DNA synthesis by two-fold when overexpressed in cells69 PubMed
vif Triad3 is proposed to act as a linker protein between HIV-1 Vif and other cellular or viral proteins PubMed
vif HIV-1 Vif mutants with amino acid substitutions in residues 105-107 are specifically unable to bind to Triad3 PubMed
vif Binding of HIV-1 Vif to Triad3 was identified using a yeast two-hybrid screen PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
ubiquitin protein ligase activity EXP
Inferred from Experiment
more info
PubMed 
Process Evidence Code Pubs
apoptotic process IEA
Inferred from Electronic Annotation
more info
 
negative regulation of type I interferon production TAS
Traceable Author Statement
more info
 
proteasome-mediated ubiquitin-dependent protein catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
protein K48-linked ubiquitination IDA
Inferred from Direct Assay
more info
PubMed 
protein catabolic process, modulating synaptic transmission IEA
Inferred from Electronic Annotation
more info
 
regulation of defense response to virus by host IDA
Inferred from Direct Assay
more info
PubMed 
regulation of interferon-beta production IDA
Inferred from Direct Assay
more info
PubMed 
viral process IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
Schaffer collateral - CA1 synapse IEA
Inferred from Electronic Annotation
more info
 
cytosol IDA
Inferred from Direct Assay
more info
 
cytosol TAS
Traceable Author Statement
more info
 
glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleus IDA
Inferred from Direct Assay
more info
 
postsynaptic endocytic zone IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
E3 ubiquitin-protein ligase RNF216
Names
RING-type E3 ubiquitin transferase RNF216
triad domain-containing protein 3
ubiquitin-conjugating enzyme 7-interacting protein 1
zinc finger protein inhibiting NF-kappa-B
NP_996994.1
NP_996999.1
XP_005249842.1
XP_011513738.1
XP_016867852.1
XP_016867853.1
XP_024302573.1
XP_024302574.1
XP_024302575.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_029374.1 RefSeqGene

    Range
    5001..166690
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_207111.3NP_996994.1  E3 ubiquitin-protein ligase RNF216 isoform a

    See identical proteins and their annotated locations for NP_996994.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1, also known as TRIAD3B) represents the longer transcript and it encodes the longer isoform (a).
    Source sequence(s)
    AC006483, AF513718, AK292396, BM970811
    Consensus CDS
    CCDS34594.1
    UniProtKB/Swiss-Prot
    Q9NWF9
    UniProtKB/TrEMBL
    A8K8N1
    Related
    ENSP00000374552.3, OTTHUMP00000208116, ENST00000389902.7, OTTHUMT00000340376
    Conserved Domains (2) summary
    pfam01485
    Location:712762
    IBR; IBR domain, a half RING-finger domain
    cd16630
    Location:570626
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
  2. NM_207116.2NP_996999.1  E3 ubiquitin-protein ligase RNF216 isoform b

    See identical proteins and their annotated locations for NP_996999.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2, also known as TRIAD3A) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform b which is shorter than isoform a.
    Source sequence(s)
    AC006483, AF513717, AK292396, BM970811
    Consensus CDS
    CCDS34595.1
    UniProtKB/Swiss-Prot
    Q9NWF9
    UniProtKB/TrEMBL
    A8K8N1
    Related
    ENSP00000404602.2, OTTHUMP00000208114, ENST00000425013.6, OTTHUMT00000340374
    Conserved Domains (1) summary
    pfam01485
    Location:655705
    IBR; IBR domain, a half RING-finger domain

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000007.14 Reference GRCh38.p12 Primary Assembly

    Range
    5620041..5781730 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024446807.1XP_024302575.1  E3 ubiquitin-protein ligase RNF216 isoform X4

    Conserved Domains (1) summary
    cd16630
    Location:135191
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
  2. XM_024446805.1XP_024302573.1  E3 ubiquitin-protein ligase RNF216 isoform X1

    Conserved Domains (2) summary
    pfam01485
    Location:712762
    IBR; IBR domain, a half RING-finger domain
    cd16630
    Location:570626
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
  3. XM_017012363.2XP_016867852.1  E3 ubiquitin-protein ligase RNF216 isoform X2

    UniProtKB/Swiss-Prot
    Q9NWF9
    Conserved Domains (1) summary
    pfam01485
    Location:655705
    IBR; IBR domain, a half RING-finger domain
  4. XM_011515436.2XP_011513738.1  E3 ubiquitin-protein ligase RNF216 isoform X4

    See identical proteins and their annotated locations for XP_011513738.1

    UniProtKB/Swiss-Prot
    Q9NWF9
    Conserved Domains (1) summary
    cd16630
    Location:135191
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
  5. XM_024446806.1XP_024302574.1  E3 ubiquitin-protein ligase RNF216 isoform X4

    Conserved Domains (1) summary
    cd16630
    Location:135191
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
  6. XM_005249785.2XP_005249842.1  E3 ubiquitin-protein ligase RNF216 isoform X1

    See identical proteins and their annotated locations for XP_005249842.1

    UniProtKB/Swiss-Prot
    Q9NWF9
    Conserved Domains (2) summary
    pfam01485
    Location:712762
    IBR; IBR domain, a half RING-finger domain
    cd16630
    Location:570626
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
  7. XM_017012364.2XP_016867853.1  E3 ubiquitin-protein ligase RNF216 isoform X3

    Conserved Domains (1) summary
    cd16630
    Location:570626
    RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_019011.4: Suppressed sequence

    Description
    NM_019011.4: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
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