U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Eprs1 glutamyl-prolyl-tRNA synthetase 1 [ Mus musculus (house mouse) ]

Gene ID: 107508, updated on 2-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
Eprs1provided by MGI
Official Full Name
glutamyl-prolyl-tRNA synthetase 1provided by MGI
Primary source
MGI:MGI:97838
See related
Ensembl:ENSMUSG00000026615 AllianceGenome:MGI:97838
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Eprs; Qprs; 2410081F06Rik; 3010002K18Rik
Summary
Enables proline-tRNA ligase activity. Involved in several processes, including cellular response to insulin stimulus; cellular response to type II interferon; and prolyl-tRNA aminoacylation. Located in plasma membrane. Part of GAIT complex and aminoacyl-tRNA synthetase multienzyme complex. Is expressed in several structures, including alimentary system; axial skeleton; cerebellum; craniocervical region bone; and liver. Human ortholog(s) of this gene implicated in hypomyelinating leukodystrophy 15. Orthologous to human EPRS1 (glutamyl-prolyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in CNS E11.5 (RPKM 17.5), liver E14 (RPKM 12.9) and 25 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See Eprs1 in Genome Data Viewer
Location:
1 H5; 1 89.5 cM
Exon count:
33
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (185093614..185160557)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (185361417..185428360)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene isoleucine-tRNA synthetase 2, mitochondrial Neighboring gene RIKEN cDNA 2010103J01 gene Neighboring gene microRNA 215 Neighboring gene STARR-seq mESC enhancer starr_03326 Neighboring gene STARR-seq mESC enhancer starr_03328 Neighboring gene 3'(2'), 5'-bisphosphate nucleotidase 1 Neighboring gene STARR-positive B cell enhancer ABC_E4403 Neighboring gene STARR-positive B cell enhancer ABC_E3350 Neighboring gene RIKEN cDNA 9630028B13 gene Neighboring gene predicted gene 2061 Neighboring gene solute carrier family 30, member 10

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA Synthetase Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy. Wu J, et al. Cells, 2023 Dec 22. PMID 38201239, Free PMC Article
  2. Translation Regulation of the Glutamyl-prolyl-tRNA Synthetase Gene EPRS through Bypass of Upstream Open Reading Frames with Noncanonical Initiation Codons. Young SK, et al. J Biol Chem, 2016 May 13. PMID 27002157, Free PMC Article
  3. Infection-specific phosphorylation of glutamyl-prolyl tRNA synthetase induces antiviral immunity. Lee EY, et al. Nat Immunol, 2016 Nov. PMID 27595231, Free PMC Article
  4. Glutamyl-prolyl-tRNA synthetase induces fibrotic extracellular matrix via both transcriptional and translational mechanisms. Song DG, et al. FASEB J, 2019 Mar. PMID 30592630
  5. The zinc-binding domain of mammalian prolyl-tRNA synthetase is indispensable for catalytic activity and organism viability. Vasu K, et al. iScience, 2021 Mar 19. PMID 33748704, Free PMC Article

See all (55) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA Synthetase Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy.
    Title: Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA Synthetase Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy.
  2. Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.
    Title: Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.
  3. The rules presented here for the regulated ribosome bypass of noncanonical initiation codons in the EPRS 5'-leader add complexity into the nature of uORF-mediated translation control mechanisms during eIF2alpha-P and additionally illustrate the roles that previously unexamined uORFs with noncanonical initiation codons can play in modulating gene expression.
    Title: Translation Regulation of the Glutamyl-prolyl-tRNA Synthetase Gene EPRS through Bypass of Upstream Open Reading Frames with Noncanonical Initiation Codons.
  4. IFN-gamma activates the macrophage GAIT system via induced phosphorylation of EPRS and L13a. L13a is phosphorylated at Ser(77) by the DAPK-ZIPK cascade, but EPRS is phosphorylated only at Ser(999).
    Title: Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages.
Submit: New GeneRIF Correction

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase binding ISO
Inferred from Sequence Orthology
more info
  
enables RNA stem-loop binding ISO
Inferred from Sequence Orthology
more info
  
enables RNA stem-loop binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables glutamate-tRNA ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables glutamate-tRNA ligase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables proline-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables proline-tRNA ligase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables proline-tRNA ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables proline-tRNA ligase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
involved_in cellular response to insulin stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to type II interferon IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cellular response to type II interferon ISO
Inferred from Sequence Orthology
more info
  
involved_in glutamyl-tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
involved_in glutamyl-tRNA aminoacylation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in negative regulation of translation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of translation ISO
Inferred from Sequence Orthology
more info
  
involved_in prolyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in prolyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in prolyl-tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of long-chain fatty acid import into cell IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of long-chain fatty acid import into cell ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of GAIT complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of GAIT complex ISO
Inferred from Sequence Orthology
more info
  
part_of aminoacyl-tRNA synthetase multienzyme complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of aminoacyl-tRNA synthetase multienzyme complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of aminoacyl-tRNA synthetase multienzyme complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
part_of ribonucleoprotein complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

Go to the top of the page Help
Preferred Names
bifunctional glutamate/proline--tRNA ligase
Names
bifunctional aminoacyl-tRNA synthetase
glutamine-proline-tRNA synthetase
NP_001344403.1
NP_084011.1
XP_017176142.1
XP_030110720.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001357474.1 → NP_001344403.1  bifunctional glutamate/proline--tRNA ligase isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC129195, AC131980
    UniProtKB/TrEMBL
    B9EIU1
    Conserved Domains (3) summary
    PRK08661
    Location:1026 → 1519
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00936
    Location:760 → 809
    WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
    cl27212
    Location:6 → 718
    GlnS; Glutamyl- or glutaminyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]

  2. NM_029735.2 → NP_084011.1  bifunctional glutamate/proline--tRNA ligase isoform 1

    See identical proteins and their annotated locations for NP_084011.1

    Status: VALIDATED

    Source sequence(s)
    AI845980, AK141072, BC058337, BC094679, CB520692, CF537682, CK635189
    Consensus CDS
    CCDS35818.1
    UniProtKB/Swiss-Prot
    E9QKC4, Q3UFJ2, Q4VC16, Q8CGC7
    UniProtKB/TrEMBL
    B9EIU1
    Related
    ENSMUSP00000045841.8, ENSMUST00000046514.13
    Conserved Domains (8) summary
    cd10309
    Location:74 → 157
    GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
    PLN02907
    Location:6 → 711
    PLN02907; glutamate-tRNA ligase
    PRK08661
    Location:1019 → 1512
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00778
    Location:1023 → 1286
    ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
    cd00862
    Location:1292 → 1512
    ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
    pfam00458
    Location:753 → 805
    WHEP-TRS; WHEP-TRS domain
    pfam00749
    Location:197 → 502
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain
    pfam03950
    Location:504 → 681
    tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    185093614..185160557
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017320653.2 → XP_017176142.1  bifunctional glutamate/proline--tRNA ligase isoform X2

    UniProtKB/TrEMBL
    B9EIU1
    Conserved Domains (4) summary
    PLN02907
    Location:2 → 454
    PLN02907; glutamate-tRNA ligase
    PRK08661
    Location:762 → 1255
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00936
    Location:496 → 545
    WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
    pfam00458
    Location:569 → 621
    WHEP-TRS; WHEP-TRS domain

  2. XM_030254860.2 → XP_030110720.1  bifunctional glutamate/proline--tRNA ligase isoform X1

    UniProtKB/TrEMBL
    B9EIU1
    Conserved Domains (4) summary
    PLN02907
    Location:2 → 461
    PLN02907; glutamate-tRNA ligase
    PRK08661
    Location:769 → 1262
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00936
    Location:503 → 552
    WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
    pfam00458
    Location:576 → 628
    WHEP-TRS; WHEP-TRS domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8CGC7.4 GenPept UniProtKB/Swiss-Prot:Q8CGC7

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous