|
Name |
Accession |
Description |
Interval |
E-value |
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
70-543 |
0e+00 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 736.30 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGaaKAVVAAPD 149
Cdd:cd05974 2 SFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRG--GAVYAAVD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05974 80 EN-----------------------------------------THADDPMLLYFTSGTTSKPKLVEHTHRSYPVGHLSTM 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADY 309
Cdd:cd05974 119 YWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFNYARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQQDLASF 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 310 KVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEI 389
Cdd:cd05974 199 DVKLREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTETTALVGNSPGQPVKAGSMGRPLPGYRVALLDPDGAPATEGEV 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 390 TLALGDARPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAE 468
Cdd:cd05974 279 ALDLGDTRPVGLMKGYAGDPDKTAHAmRGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAE 358
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 469 AAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVtELPKTISGKIRRVHLRRLE 543
Cdd:cd05974 359 AAVVPSPDPVRLSVPKAFIVLRAGYEPSPETALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVELRRRE 432
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
26-551 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 547.02 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 26 RWPDPAPFNWALDWFDAELATNADsrdRAALwIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVP 105
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAEGRGD---KVAL-IWEGEDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPE 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 106 LWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQV---------AKFADL--GGDSLIRIVV------G 168
Cdd:COG0365 77 AVIAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLrggkvidlkEKVDEAleELPSLEHVIVvgrtgaD 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 169 SPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM-FWLGLQPGDVHLNISSPG 247
Cdd:COG0365 157 VPMEGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAkYVLDLKPGDVFWCTADIG 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 248 WAKHAWSCFFAPWNAGATVFVVN-QPRF-DAKSLLATIGRCGVTTLCAPPTVWRMFIQ---EKLADYKVS-LREVCGAGE 321
Cdd:COG0365 237 WATGHSYIVYGPLLNGATVVLYEgRPDFpDPGRLWELIEKYGVTVFFTAPTAIRALMKagdEPLKKYDLSsLRLLGSAGE 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 322 PLNPEVIDQVNAAWGATIRDGYGQTETTA-MVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEI-TLALGDARPa 399
Cdd:COG0365 317 PLNPEVWEWWYEAVGVPIVDGWGQTETGGiFISNLPGLPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEgELVIKGPWP- 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 400 GLMQGYQGEGGK----LSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSP 475
Cdd:COG0365 396 GMFRGYWNDPERyretYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVP 475
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 476 DAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDDDRADT 551
Cdd:COG0365 476 DEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAEGRPLGDT 551
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
70-540 |
5.44e-180 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 514.58 E-value: 5.44e-180
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVaapd 149
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIV---- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQrSYPVGALSTM 229
Cdd:cd05972 78 -------------------------------------------TDAEDPALIYFTSGTTGLPKGVLHTH-SYPLGHIPTA 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 -FWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLAD 308
Cdd:cd05972 114 aYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRFDAERILELLERYGVTSFCGPPTAYRMLIKQDLSS 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 309 YK-VSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGH---PA 384
Cdd:cd05972 194 YKfSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGSMGRPTPGYDVAIIDDDGRelpPG 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 385 KEGEITLALGdarPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEH 463
Cdd:cd05972 274 EEGDIAIKLP---PPGLFLGYVGDPEKTEASiRGDYYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEH 350
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 91684220 464 EAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:cd05972 351 PAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELR 427
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
25-545 |
1.00e-150 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 443.86 E-value: 1.00e-150
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 25 FRWPDPAPFNWALDWFDAelaTNADSRDRAAL-WIVDgiTGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNV 103
Cdd:cd05970 8 FSINVPENFNFAYDVVDA---MAKEYPDKLALvWCDD--AGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 104 VPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAA-----PDQVAK-FADLGGDSLIRIVVGSPQDGWRGY 177
Cdd:cd05970 83 YEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIaedniPEEIEKaAPECPSKPKLVWVGDPVPEGWIDF 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 178 D-EASQQSDSFT-PDGPTQA--DDPMLLYFTSGTTAKPKLVRHSQrSYPVGALST-MFWLGLQPGDVHLNISSPGWAKHA 252
Cdd:cd05970 163 RkLIKNASPDFErPTANSYPcgEDILLVYFSSGTTGMPKMVEHDF-TYPLGHIVTaKYWQNVREGGLHLTVADTGWGKAV 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 253 WSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKVS-LREVCGAGEPLNPEVIDQV 331
Cdd:cd05970 242 WGKIYGQWIAGAAVFVYDYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSRYDLSsLRYCTTAGEALNPEVFNTF 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 332 NAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAK---EGEITLALGDARPAGLMQGYQGE 408
Cdd:cd05970 322 KEKTGIKLMEGFGQTETTLTIATFPWMEPKPGSMGKPAPGYEIDLIDREGRSCEageEGEIVIRTSKGKPVGLFGGYYKD 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 409 GGKLSGAEGD-IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYV 487
Cdd:cd05970 402 AEKTAEVWHDgYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATI 481
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 91684220 488 LLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRrlEHD 545
Cdd:cd05970 482 VLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR--ERD 537
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
30-543 |
1.31e-148 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 438.05 E-value: 1.31e-148
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 30 PAPFNWALDWFDaELATNADSRDRA---ALWIVDGiTGVELKPSFATMSKRSNQVANYLR-AQGLKRGDHLLLLLGNVVP 105
Cdd:cd05928 2 PEYFNFASDVLD-QWADKEKAGKRPpnpALWWVNG-KGDEVKWSFRELGSLSRKAANVLSgACGLQRGDRVAVILPRVPE 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 106 LWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAApDQVAKFAD---LGGDSL-IRIVVG-SPQDGWRGYDEA 180
Cdd:cd05928 80 WWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTS-DELAPEVDsvaSECPSLkTKLLVSeKSRDGWLNFKEL 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 181 SQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGA-LSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAP 259
Cdd:cd05928 159 LNEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLkVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEP 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 260 WNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKV-SLREVCGAGEPLNPEVIDQVNAAWGAT 338
Cdd:cd05928 239 WIQGACVFVHHLPRFDPLVILKTLSSYPITTFCGAPTVYRMLVQQDLSSYKFpSLQHCVTGGEPLNPEVLEKWKAQTGLD 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 339 IRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGH---PAKEGEITLALGDARPAGLMQGYQGEGGKLSGA 415
Cdd:cd05928 319 IYEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDNGNvlpPGTEGDIGIRVKPIRPFGLFSGYVDNPEKTAAT 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 416 E-GDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAE 494
Cdd:cd05928 399 IrGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFL 478
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 91684220 495 RSRDTALS--IFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLE 543
Cdd:cd05928 479 SHDPEQLTkeLQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRDKE 529
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
38-549 |
8.09e-107 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 328.31 E-value: 8.09e-107
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 38 DWFDAELATNADsrdraALWIVDGitGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLG 117
Cdd:COG0318 3 DLLRRAAARHPD-----RPALVFG--GRRL--TYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAG 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 118 IVVIPATTLLTPDELRDRLDRGAAKAVVAApdqvakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgptqadd 197
Cdd:COG0318 74 AVVVPLNPRLTAEELAYILEDSGARALVTA-------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 198 pmLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAkHAWS-CFFAPWNAGATVFVVnqPRFDA 276
Cdd:COG0318 104 --LILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHV-FGLTvGLLAPLLAGATLVLL--PRFDP 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 277 KSLLATIGRCGVTTLCAPPTVWRMFIQ-EKLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGN 354
Cdd:COG0318 179 ERVLELIERERVTVLFGVPTMLARLLRhPEFARYDLSsLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVTV 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 355 SP--GQPVKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGD--ARPAGLMQGY--QGEGGKLSGAEGdIYRSGDVAFA 428
Cdd:COG0318 259 NPedPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEV----GEivVRGPNVMKGYwnDPEATAEAFRDG-WLRTGDLGRL 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 429 DDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAErsrDTALSIFRHMQ 508
Cdd:COG0318 334 DEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAE---LDAEELRAFLR 410
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 91684220 509 QRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDDDRA 549
Cdd:COG0318 411 ERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAAGALE 451
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
70-543 |
2.67e-104 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 321.37 E-value: 2.67e-104
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKfadlggdslirivvgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05969 82 LYER---------------------------------------TDPEDPTLLHYTSGTTGTPKGVLHVHDAMIFYYFTGK 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQ---EKL 306
Cdd:cd05969 123 YVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTN-VVYEGRFDAESWYGIIERVKVTVWYTAPTAIRMLMKegdELA 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 307 ADYKV-SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTET-TAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPA 384
Cdd:cd05969 202 RKYDLsSLRFIHSVGEPLNPEAIRWGMEVFGVPIHDTWWQTETgSIMIANYPCMPIKPGSMGKPLPGVKAAVVDENGNEL 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 385 KEGEI-TLALGDARPAgLMQGYQGEGGK--LSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILL 461
Cdd:cd05969 282 PPGTKgILALKPGWPS-MFRGIWNDEERykNSFIDG-WYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALM 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 462 EHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:cd05969 360 EHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKA 439
|
..
gi 91684220 542 LE 543
Cdd:cd05969 440 KE 441
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
16-537 |
6.77e-101 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 316.84 E-value: 6.77e-101
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 16 TDYAAAVAGFRWPD-PAPFNW-----------ALDwfdaelaTNADS--RDRAALWIVDGitGVELKPSFATMSKRSNQV 81
Cdd:PRK04319 16 KDYEETYATFSWEEvEKEFSWletgkvniayeAID-------RHADGgrKDKVALRYLDA--SRKEKYTYKELKELSNKF 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 82 ANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAK--FADLgg 159
Cdd:PRK04319 87 ANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLERkpADDL-- 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 160 DSLIRIVV----GSPQDGWRGYDEASQQ-SDSFTPDgPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGL 234
Cdd:PRK04319 165 PSLKHVLLvgedVEEGPGTLDFNALMEQaSDEFDIE-WTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYQTGKYVLDL 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 235 QPGDVHLNISSPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFI---QEKLADYKV 311
Cdd:PRK04319 244 HEDDVYWCTADPGWVTGTSYGIFAPWLNGATN-VIDGGRFSPERWYRILEDYKVTVWYTAPTAIRMLMgagDDLVKKYDL 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 312 S-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTA-MVGNSPGQPVKIGSMGRPLPGY---VVRITDADGHPAKE 386
Cdd:PRK04319 323 SsLRHILSVGEPLNPEVVRWGMKVFGLPIHDNWWMTETGGiMIANYPAMDIKPGSMGKPLPGIeaaIVDDQGNELPPNRM 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 387 GEitLALGDARPAgLMQGYQGEGGK-LSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEA 465
Cdd:PRK04319 403 GN--LAIKKGWPS-MMRGIWNNPEKyESYFAGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPA 479
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 91684220 466 VAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKI-RRV 537
Cdd:PRK04319 480 VAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKImRRV 552
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
70-541 |
7.35e-99 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 307.14 E-value: 7.35e-99
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:cd05973 2 TFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTDAA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADlggdslirivvgspqdgwrgydeasqqsdsftpdgptqadDPMLLYFTSGTTAKPKLVRHsqrsyPVGALSTM 229
Cdd:cd05973 82 NRHKLDS----------------------------------------DPFVMMFTSGTTGLPKGVPV-----PLRALAAF 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 -----FWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGaTVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQ- 303
Cdd:cd05973 117 gaylrDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALG-HPTILLEGGFSVESTWRVIERLGVTNLAGSPTAYRLLMAa 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 --EKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGN--SPGQPVKIGSMGRPLPGYVVRITDA 379
Cdd:cd05973 196 gaEVPARPKGRLRRVSSAGEPLTPEVIRWFDAALGVPIHDHYGQTELGMVLANhhALEHPVHAGSAGRAMPGWRVAVLDD 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DGHPAKEGEITLALGDARPAGLM--QGYQGEGGKlsGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:cd05973 276 DGDELGPGEPGRLAIDIANSPLMwfRGYQLPDTP--AIDGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVE 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRV 537
Cdd:cd05973 354 SALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRF 433
|
....
gi 91684220 538 HLRR 541
Cdd:cd05973 434 LLRR 437
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
197-535 |
2.11e-87 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 274.16 E-value: 2.11e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHaWSCFFAPWNAGATVFVVnqPRFDA 276
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGG-LFGLLGALLAGGTVVLL--PKFDP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 277 KSLLATIGRCGVTTLCAPPTVWRMFI-QEKLADY-KVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV-- 352
Cdd:cd04433 78 EAALELIEREKVTILLGVPTLLARLLkAPESAGYdLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTETGGTVat 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 GNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGD--ARPAGLMQGYQGEGGKLSGAEGDI-YRSGDVAFAD 429
Cdd:cd04433 158 GPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEI----GElvVRGPSVMKGYWNNPEATAAVDEDGwYRTGDLGRLD 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 430 DDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTalsIFRHMQQ 509
Cdd:cd04433 234 EDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAEE---LRAHVRE 310
|
330 340
....*....|....*....|....*.
gi 91684220 510 RLAPFKRIRKLELVTELPKTISGKIR 535
Cdd:cd04433 311 RLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
70-540 |
4.77e-84 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 270.78 E-value: 4.77e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:cd05959 31 TYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYLEDSRARVVVVSGE 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGD---SLIRIVVGSPQDGWRGY----DEASQQSDSFTPdGPTQADDPMLLYFTSGTTAKPKLVRHSQRS-Y 221
Cdd:cd05959 111 LAPVLAAALTKsehTLVVLIVSGGAGPEAGAlllaELVAAEAEQLKP-AATHADDPAFWLYSSGSTGRPKGVVHLHADiY 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 222 PVGALSTMFWLGLQPGDVHLNIsspgwAK--HAW----SCFFAPWNAGATVFVVNQPRfdAKSLLATIGRCGVTTLCAPP 295
Cdd:cd05959 190 WTAELYARNVLGIREDDVCFSA-----AKlfFAYglgnSLTFPLSVGATTVLMPERPT--PAAVFKRIRRYRPTVFFGVP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 296 TVWR-MFIQEKLADYK-VSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAM-VGNSPGQpVKIGSMGRPLPGY 372
Cdd:cd05959 263 TLYAaMLAAPNLPSRDlSSLRLCVSAGEALPAEVGERWKARFGLDILDGIGSTEMLHIfLSNRPGR-VRYGTTGKPVPGY 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 373 VVRITDADGHPAKEGEITLALGDARPAGLMQGYQGEGGKLSgAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRIS 452
Cdd:cd05959 342 EVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDT-FQGEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVS 420
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 453 PFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISG 532
Cdd:cd05959 421 PFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATG 500
|
....*...
gi 91684220 533 KIRRVHLR 540
Cdd:cd05959 501 KIQRFKLR 508
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
70-541 |
3.07e-83 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 266.61 E-value: 3.07e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapd 149
Cdd:cd05971 8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT--- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsftpDGptqADDPMLLYFTSGTTAKPKLVRHSQRSY--PVGALS 227
Cdd:cd05971 85 ----------------------------------------DG---SDDPALIIYTSGTTGPPKGALHAHRVLlgHLPGVQ 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 228 TMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEK-- 305
Cdd:cd05971 122 FPFNLFPRDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKFDPKAALDLMSRYGVTTAFLPPTALKMMRQQGeq 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 LADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPG-QPVKIGSMGRPLPGYVVRITDADGH-- 382
Cdd:cd05971 202 LKHAQVKLRAIATGGESLGEELLGWAREQFGVEVNEFYGQTECNLVIGNCSAlFPIKPGSMGKPIPGHRVAIVDDNGTpl 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 383 -PAKEGEITLALGDarPAGLMQGYQGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILL 461
Cdd:cd05971 282 pPGEVGEIAVELPD--PVAFLGYWNNPSATEKKMAGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLL 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 462 EHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:cd05971 360 KHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
70-541 |
7.02e-81 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 262.82 E-value: 7.02e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK06187 33 TYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIRLKPEEIAYILNDAEDRVVLVDSE 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGD-SLIRIVV-------GSPQDGWRGYDE-ASQQSDSFtPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRS 220
Cdd:PRK06187 113 FVPLLAAILPQlPTVRTVIvegdgpaAPLAPEVGEYEElLAAASDTF-DFPDIDENDAAAMLYTSGTTGHPKGVVLSHRN 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 221 YPVGALSTMFWLGLQPGDVHLnISSPGWAKHAWSCFFAPWNAGATVfvVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRM 300
Cdd:PRK06187 192 LFLHSLAVCAWLKLSRDDVYL-VIVPMFHVHAWGLPYLALMAGAKQ--VIPRRFDPENLLDLIETERVTFFFAVPTIWQM 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 301 FIQEKLA---DYKvSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETT-AMVGNSP-----GQPVKIGSMGRPLPG 371
Cdd:PRK06187 269 LLKAPRAyfvDFS-SLRLVIYGGAALPPALLREFKEKFGIDLVQGYGMTETSpVVSVLPPedqlpGQWTKRRSAGRPLPG 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 372 YVVRITDADG----HPAKE-GEITLalgdaRPAGLMQGYQGEGGKlsGAE---GDIYRSGDVAFADDDGYLTFVGRTDDV 443
Cdd:PRK06187 348 VEARIVDDDGdelpPDGGEvGEIIV-----RGPWLMQGYWNRPEA--TAEtidGGWLHTGDVGYIDEDGYLYITDRIKDV 420
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 444 FKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELV 523
Cdd:PRK06187 421 IISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAK---ELRAFLRGRLAKFKLPKRIAFV 497
|
490
....*....|....*...
gi 91684220 524 TELPKTISGKIRRVHLRR 541
Cdd:PRK06187 498 DELPRTSVGKILKRVLRE 515
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
70-540 |
8.44e-78 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 253.25 E-value: 8.44e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAApd 149
Cdd:cd05936 26 TYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYTPRELEHILNDSGAKALIVA-- 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvAKFADLGgdslirivvgspqdgwrgydeasqQSDSFTPDGPT-QADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALST 228
Cdd:cd05936 104 --VSFTDLL------------------------AAGAPLGERVAlTPEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQI 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 229 MFWLG--LQPGDVHLNISsPGWakHAWS---CFFAPWNAGATVFVVnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQ 303
Cdd:cd05936 158 KAWLEdlLEGDDVVLAAL-PLF--HVFGltvALLLPLALGATIVLI--PRFRPIGVLKEIRKHRVTIFPGVPTMYIALLN 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 EKlADYKV---SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV-GNSPGQPVKIGSMGRPLPGYVVRITDA 379
Cdd:cd05936 233 AP-EFKKRdfsSLRLCISGGAPLPVEVAERFEELTGVPIVEGYGLTETSPVVaVNPLDGPRKPGSIGIPLPGTEVKIVDD 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DGHPAKEGEItlalGDARPAG--LMQGY--QGEGGKLSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFE 455
Cdd:cd05936 312 DGEELPPGEV----GELWVRGpqVMKGYwnRPEETAEAFVDG-WLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPRE 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 456 LESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIR 535
Cdd:cd05936 387 VEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKIL 463
|
....*
gi 91684220 536 RVHLR 540
Cdd:cd05936 464 RRELR 468
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
58-534 |
2.37e-74 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 244.81 E-value: 2.37e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 58 IVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLD 137
Cdd:cd05911 2 QIDADTGKEL--TYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLK 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 138 RGAAKAVVAAPDQVAK----FADLGGDSLIRIV------VGSPQDGWRGYDEAsQQSDSFTPDGpTQADDPMLLYFTSGT 207
Cdd:cd05911 80 ISKPKVIFTDPDGLEKvkeaAKELGPKDKIIVLddkpdgVLSIEDLLSPTLGE-EDEDLPPPLK-DGKDDTAAILYSSGT 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 208 TAKPKLVRHSQRSYPVGALS--TMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNaGATVFVVnqPRFDAKSLLATIGR 285
Cdd:cd05911 158 TGLPKGVCLSHRNLIANLSQvqTFLYGNDGSNDVILGFLPLYHIYGLFTTLASLLN-GATVIIM--PKFDSELFLDLIEK 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 286 CGVTTLC-APPTVWRMFIQEKLADYKV-SLREVCGAGEPLNPEVIDQVNAAWG-ATIRDGYGQTETTAMVGNSPGQPVKI 362
Cdd:cd05911 235 YKITFLYlVPPIAAALAKSPLLDKYDLsSLRVILSGGAPLSKELQELLAKRFPnATIKQGYGMTETGGILTVNPDGDDKP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 363 GSMGRPLPGYVVRITDADGH----PAKEGEITLalgdaRPAGLMQGYQG--EGGKLSGAEGDIYRSGDVAFADDDGYLTF 436
Cdd:cd05911 315 GSVGRLLPNVEAKIVDDDGKdslgPNEPGEICV-----RGPQVMKGYYNnpEATKETFDEDGWLHTGDIGYFDEDGYLYI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 437 VGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAErsrDTALSIFRHMQQRLAPFKR 516
Cdd:cd05911 390 VDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEK---LTEKEVKDYVAKKVASYKQ 466
|
490
....*....|....*....
gi 91684220 517 IRK-LELVTELPKTISGKI 534
Cdd:cd05911 467 LRGgVVFVDEIPKSASGKI 485
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
70-540 |
5.66e-74 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 244.36 E-value: 5.66e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:TIGR02262 32 SYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVSGA 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGDS---LIRIVVGSPQDGWRGY-DEASQQSDSFTPdGPTQADDPMLLYFTSGTTAKPKLVRHSQRS-YPVG 224
Cdd:TIGR02262 112 LLPVIKAALGKSphlEHRVVVGRPEAGEVQLaELLATESEQFKP-AATQADDPAFWLYSSGSTGMPKGVVHTHSNpYWTA 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 225 ALSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWR-MFIQ 303
Cdd:TIGR02262 191 ELYARNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATT-VLMGERPTPDAVFDRLRRHQPTIFYGVPTLYAaMLAD 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 EKL-ADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAM-VGNSPGQpVKIGSMGRPLPGYVVRITDADG 381
Cdd:TIGR02262 270 PNLpSEDQVRLRLCTSAGEALPAEVGQRWQARFGVDIVDGIGSTEMLHIfLSNLPGD-VRYGTSGKPVPGYRLRLVGDGG 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 382 HPAKEGEITLALGDARPAGLMqgYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESIL 460
Cdd:TIGR02262 349 QDVADGEPGELLISGPSSATM--YWNNRAKSRDTfQGEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESAL 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 461 LEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:TIGR02262 427 IQHPAVLEAAVVGVADEDGLIKPKAFVVLRPGQTALET---ELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLR 503
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
52-447 |
5.88e-71 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 233.74 E-value: 5.88e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAALWIVDGITgvelkPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDE 131
Cdd:pfam00501 10 DKTALEVGEGRR-----LTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRLPAEE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 132 LRDRLDRGAAKAVVAAPDQVAKFADLGGDSL------IRIVVGSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTS 205
Cdd:pfam00501 85 LAYILEDSGAKVLITDDALKLEELLEALGKLevvklvLVLDRDPVLKEEPLPEEAKPADVPPPPPPPPDPDDLAYIIYTS 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 206 GTTAKPKLVRHSQRSYpVGALSTMFW-----LGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGAT-VFVVNQPRFDAKSL 279
Cdd:pfam00501 165 GTTGKPKGVMLTHRNL-VANVLSIKRvrprgFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGATvVLPPGFPALDPAAL 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 280 LATIGRCGVTTLCAPPTVWRMFIQEKLADYKV--SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV---GN 354
Cdd:pfam00501 244 LELIERYKVTVLYGVPTLLNMLLEAGAPKRALlsSLRLVLSGGAPLPPELARRFRELFGGALVNGYGLTETTGVVttpLP 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 355 SPGQPVKIGSMGRPLPGYVVRITDADG----HPAKEGEITLalgdaRPAGLMQGYQGEGGKLSGA--EGDIYRSGDVAFA 428
Cdd:pfam00501 324 LDEDLRSLGSVGRPLPGTEVKIVDDETgepvPPGEPGELCV-----RGPGVMKGYLNDPELTAEAfdEDGWYRTGDLGRR 398
|
410
....*....|....*....
gi 91684220 429 DDDGYLTFVGRTDDVFKSS 447
Cdd:pfam00501 399 DEDGYLEIVGRKKDQIKLG 417
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
70-536 |
3.63e-69 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 229.80 E-value: 3.63e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVaapd 149
Cdd:cd17631 22 TYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEVAYILADSGAKVLF---- 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgptqaDDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd17631 98 ----------------------------------------------DDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNAL 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLnISSPGWAKHAWSCFFAP-WNAGATVFVVnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQ-EKLA 307
Cdd:cd17631 132 AALDLGPDDVLL-VVAPLFHIGGLGVFTLPtLLRGGTVVIL--RKFDPETVLDLIERHRVTSFFLVPTMIQALLQhPRFA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 308 DYKVS-LREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMV-GNSPG-QPVKIGSMGRPLPGYVVRITDADGHPA 384
Cdd:cd17631 209 TTDLSsLRAVIYGGAPMPERLLRALQAR-GVKFVQGYGMTETSPGVtFLSPEdHRRKLGSAGRPVFFVEVRIVDPDGREV 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 385 KEGEItlalGD--ARPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILL 461
Cdd:cd17631 288 PPGEV----GEivVRGPHVMAGYWNRPEATAAAfRDGWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLY 363
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 462 EHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERsrdTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:cd17631 364 EHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAEL---DEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
70-540 |
5.58e-69 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 228.72 E-value: 5.58e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapd 149
Cdd:cd05934 5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgptqadDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05934 82 -----------------------------------------------DPASILYTSGTTGPPKGVVITHANLTFAGYYSA 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDV--------HLNISSPGWAKhAWSCffapwnaGATVfvVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMF 301
Cdd:cd05934 115 RRFGLGEDDVyltvlplfHINAQAVSVLA-ALSV-------GATL--VLLPRFSASRFWSDVRRYGATVTNYLGAMLSYL 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 302 I-QEKLADYKVS-LREVCGAGEPlnPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDA 379
Cdd:cd05934 185 LaQPPSPDDRAHrLRAAYGAPNP--PELHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYEVRIVDD 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DGHPAKEGEI-TLALGDARPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:cd05934 263 DGQELPAGEPgELVIRGLRGWGFFKGYYNMPEATAEAmRNGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVE 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAersRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRV 537
Cdd:cd05934 343 RAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGE---TLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKA 419
|
...
gi 91684220 538 HLR 540
Cdd:cd05934 420 QLR 422
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
70-540 |
1.56e-68 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 228.12 E-value: 1.56e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVaapd 149
Cdd:cd05919 12 TYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVV---- 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSYP--VGALS 227
Cdd:cd05919 88 -------------------------------------------TSADDIAYLLYSSGTTGPPKGVMHAHRDPLlfADAMA 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 228 TMFwLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKL- 306
Cdd:cd05919 125 REA-LGLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASA-VLNPGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAg 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 307 -ADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAM-VGNSPGQpVKIGSMGRPLPGYVVRITDADGHPA 384
Cdd:cd05919 203 sPDALRSLRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGHIfLSNRPGA-WRLGSTGRPVPGYEIRLVDEEGHTI 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 385 KEGEITLALgdARPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEH 463
Cdd:cd05919 282 PPGEEGDLL--VRGPSAAVGYWNNPEKSRATfNGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQH 359
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 91684220 464 EAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:cd05919 360 PAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
15-534 |
6.88e-65 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 222.45 E-value: 6.88e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 15 RTDYAAAVAGFRWPDPAPFNWALDWFDAELATNADsrdRAALwIVDGITGVELKP-SFATMSKRSNQVANYLRAQGLKRG 93
Cdd:cd17634 34 NTSFAPGAPSIKWFEDATLNLAANALDRHLRENGD---RTAI-IYEGDDTSQSRTiSYRELHREVCRFAGTLLDLGVKKG 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 94 DHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD------------QVAKFADLGGDS 161
Cdd:cd17634 110 DRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITADGgvragrsvplkkNVDDALNPNVTS 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 162 LIRIVV----GSPQDG----WRGYDE-ASQQSDSFTPDgPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM-FW 231
Cdd:cd17634 190 VEHVIVlkrtGSDIDWqegrDLWWRDlIAKASPEHQPE-AMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMkYV 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 232 LGLQPGDVHLNISSPGW-AKHAWsCFFAPWNAGATVF----VVNQPrfDAKSLLATIGRCGVTTLCAPPTVWRMFIQEK- 305
Cdd:cd17634 269 FDYGPGDIYWCTADVGWvTGHSY-LLYGPLACGATTLlyegVPNWP--TPARMWQVVDKHGVNILYTAPTAIRALMAAGd 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 --LADYKVS-LREVCGAGEPLNPEVID---QVNAAWGATIRDGYGQTETT-AMVGNSPG-QPVKIGSMGRPLPGYVVRIT 377
Cdd:cd17634 346 daIEGTDRSsLRILGSVGEPINPEAYEwywKKIGKEKCPVVDTWWQTETGgFMITPLPGaIELKAGSATRPVFGVQPAVV 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 378 DADGHPAKEG-EITLALGDARPAGLMQGY----QGEGGKLSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRIS 452
Cdd:cd17634 426 DNEGHPQPGGtEGNLVITDPWPGQTRTLFgdheRFEQTYFSTFKG-MYFSGDGARRDEDGYYWITGRSDDVINVAGHRLG 504
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 453 PFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISG 532
Cdd:cd17634 505 TAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSG 584
|
..
gi 91684220 533 KI 534
Cdd:cd17634 585 KI 586
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
70-540 |
2.88e-64 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 216.96 E-value: 2.88e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLW-ETMLAAIKLGIVVIPATTLLTPDELRDRLDRgAAKAVVAAP 148
Cdd:cd05958 12 TYRDLLALANRIANVLVGELGIVPGNRVLLRGSNSPELvACWFGIQKAGAIAVATMPLLRPKELAYILDK-ARITVALCA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 149 DQVakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGA-LS 227
Cdd:cd05958 91 HAL-----------------------------------------TASDDICILAFTSGTTGAPKATMHFHRDPLASAdRY 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 228 TMFWLGLQPGDVHLnisspGWAKHAWS-----CFFAPWNAGATVFVVnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMFI 302
Cdd:cd05958 130 AVNVLRLREDDRFV-----GSPPLAFTfglggVLLFPFGVGASGVLL--EEATPDLLLSAIARYKPTVLFTAPTAYRAML 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 QEKLADYKV--SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAM-VGNSPGQpVKIGSMGRPLPGYVVRITDA 379
Cdd:cd05958 203 AHPDAAGPDlsSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMFHIfISARPGD-ARPGATGKPVPGYEAKVVDD 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DGHPAKEGEITLaLGDARPAGLMqgYQGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESI 459
Cdd:cd05958 282 EGNPVPDGTIGR-LAVRGPTGCR--YLADKRQRTYVQGGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDV 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 460 LLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHL 539
Cdd:cd05958 359 LLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFAL 438
|
.
gi 91684220 540 R 540
Cdd:cd05958 439 R 439
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
70-542 |
2.33e-63 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 219.04 E-value: 2.33e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIV--VIPATtlLTPDELRDRLDRGAAKAVVAA 147
Cdd:TIGR02188 90 TYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIhsVVFGG--FSAEALADRINDAGAKLVITA 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 148 ------------PDQVAKFADLGGDSLIRIVV----GSPQDGW---RGY---DEASQQSDSFTPDgPTQADDPMLLYFTS 205
Cdd:TIGR02188 168 deglrggkviplKAIVDEALEKCPVSVEHVLVvrrtGNPVVPWvegRDVwwhDLMAKASAYCEPE-PMDSEDPLFILYTS 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 206 GTTAKPKLVRHSQRSYPVGALSTMFWL-GLQPGDVHLNISSPGWAK-HAWSCFfAPWNAGATVFVV-NQPRF-DAKSLLA 281
Cdd:TIGR02188 247 GSTGKPKGVLHTTGGYLLYAAMTMKYVfDIKDGDIFWCTADVGWITgHSYIVY-GPLANGATTVMFeGVPTYpDPGRFWE 325
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 282 TIGRCGVTTLCAPPTVWRMFIqeKLADYKV------SLREVCGAGEPLNPEvidqvnaAW-------GAT---IRDGYGQ 345
Cdd:TIGR02188 326 IIEKHKVTIFYTAPTAIRALM--RLGDEWVkkhdlsSLRLLGSVGEPINPE-------AWmwyykvvGKErcpIVDTWWQ 396
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 346 TET-TAMVGNSPG-QPVKIGSMGRPLPGYVVRITDADGHPAK--EGEITLALGDARPaGLMQGYQGEGGK-----LSGAE 416
Cdd:TIGR02188 397 TETgGIMITPLPGaTPTKPGSATLPFFGIEPAVVDEEGNPVEgpGEGGYLVIKQPWP-GMLRTIYGDHERfvdtyFSPFP 475
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 417 GdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERS 496
Cdd:TIGR02188 476 G-YYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPD 554
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 91684220 497 RDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:TIGR02188 555 DELRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKI 600
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
51-551 |
1.32e-62 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 216.66 E-value: 1.32e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 51 RDRAAL-WIVDGiTGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIV--VIPATtlL 127
Cdd:cd05966 67 GDKVAIiWEGDE-PDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVhsVVFAG--F 143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPDELRDRLDRGAAKAVVAApDQV---AKFADL---------GGDSLIRIVV----GSP------QDGWrGYDEASQQSD 185
Cdd:cd05966 144 SAESLADRINDAQCKLVITA-DGGyrgGKVIPLkeivdealeKCPSVEKVLVvkrtGGEvpmtegRDLW-WHDLMAKQSP 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 186 SFTPDgPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFW-LGLQPGDVHLNISSPGW-AKHAWSCFfAPWNAG 263
Cdd:cd05966 222 ECEPE-WMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFKYvFDYHPDDIYWCTADIGWiTGHSYIVY-GPLANG 299
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 264 ATVFVV----NQPrfDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYK----VSLREVCGAGEPLNPEvidqvnaAW 335
Cdd:cd05966 300 ATTVMFegtpTYP--DPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKkhdlSSLRVLGSVGEPINPE-------AW 370
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 336 -------G---ATIRDGYGQTET-TAMVGNSPG-QPVKIGSMGRPLPGYVVRITDADGHP-AKEGEITLALGDARPaGLM 402
Cdd:cd05966 371 mwyyeviGkerCPIVDTWWQTETgGIMITPLPGaTPLKPGSATRPFFGIEPAILDEEGNEvEGEVEGYLVIKRPWP-GMA 449
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 403 QGYQG-----EGGKLSgAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA 477
Cdd:cd05966 450 RTIYGdheryEDTYFS-KFPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHD 528
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 478 IKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKI-RRVhLRRL-EHDDDRADT 551
Cdd:cd05966 529 IKGEAIYAFVTLKDGEEPSDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKImRRI-LRKIaAGEEELGDT 603
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
51-541 |
1.84e-62 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 214.02 E-value: 1.84e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 51 RDRAALwiVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPD 130
Cdd:cd05904 19 PSRPAL--IDAATGRAL--TYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPA 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 131 ELRDRLDRGAAKAVVAAPDQVAKFADLGgdsLIRIVVGS-PQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTA 209
Cdd:cd05904 95 EIAKQVKDSGAKLAFTTAELAEKLASLA---LPVVLLDSaEFDSLSFSDLLFEADEAEPPVVVIKQDDVAALLYSSGTTG 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 210 KPKLVRHSQRSYPVGALSTMFWLGLQ--PGDVHL------NISSPGWakhawsCFFAPWNAGATVFVVnqPRFDAKSLLA 281
Cdd:cd05904 172 RSKGVMLTHRNLIAMVAQFVAGEGSNsdSEDVFLcvlpmfHIYGLSS------FALGLLRLGATVVVM--PRFDLEELLA 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 282 TIGRCGVTTL-CAPPTVWRMFIQEKLADYKV-SLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETT---AMVGNS 355
Cdd:cd05904 244 AIERYKVTHLpVVPPIVLALVKSPIVDKYDLsSLRQIMSGAAPLGKELIEAFRAKFpNVDLGQGYGMTESTgvvAMCFAP 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 356 PGQPVKIGSMGRPLPGYVVRITDAD-GH---PAKEGEITLalgdaRPAGLMQGYQG--EGGKLSGAEGDIYRSGDVAFAD 429
Cdd:cd05904 324 EKDRAKYGSVGRLVPNVEAKIVDPEtGEslpPNQTGELWI-----RGPSIMKGYLNnpEATAATIDKEGWLHTGDLCYID 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 430 DDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadaeRSRDTALS---IFRH 506
Cdd:cd05904 399 EDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVV------RKPGSSLTedeIMDF 472
|
490 500 510
....*....|....*....|....*....|....*
gi 91684220 507 MQQRLAPFKRIRKLELVTELPKTISGKIrrvhLRR 541
Cdd:cd05904 473 VAKQVAPYKKVRKVAFVDAIPKSPSGKI----LRK 503
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
70-536 |
8.75e-61 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 207.77 E-value: 8.75e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLG---NVVPlweTMLAAIKLGIVVIPattlLTPDELRDRLdrgaakavva 146
Cdd:cd05930 14 TYAELDARANRLARYLRERGVGPGDLVAVLLErslEMVV---AILAVLKAGAAYVP----LDPSYPAERL---------- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 147 apdqvakfADLGGDSLIRIVVgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRsypvGAL 226
Cdd:cd05930 77 --------AYILEDSGAKLVL-------------------------TDPDDLAYVIYTSGSTGKPKGVMVEHR----GLV 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 227 STMFW----LGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVV-NQPRFDAKSLLATIGRCGVTTLCAPPTVWRMF 301
Cdd:cd05930 120 NLLLWmqeaYPLTPGDRVLQFTSFSFDVSVWE-IFGALLAGATLVVLpEEVRKDPEALADLLAEEGITVLHLTPSLLRLL 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 302 IQEKLADYKVSLREVCGAGEPLNPEVIDQVNAA-WGATIRDGYGQTETTAMV-------GNSPGQPVKIgsmGRPLPGYV 373
Cdd:cd05930 199 LQELELAALPSLRLVLVGGEALPPDLVRRWRELlPGARLVNLYGPTEATVDAtyyrvppDDEEDGRVPI---GRPIPNTR 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 374 VRITDADGHPAK---EGEITLAlGdarpAGLMQGYQGEGG-------KLSGAEGD-IYRSGDVAFADDDGYLTFVGRTDD 442
Cdd:cd05930 276 VYVLDENLRPVPpgvPGELYIG-G----AGLARGYLNRPEltaerfvPNPFGPGErMYRTGDLVRWLPDGNLEFLGRIDD 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 443 VFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVllTADAERSRDTAlSIFRHMQQRLAPFKRIRKLEL 522
Cdd:cd05930 351 QVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDGEKRLVAYV--VPDEGGELDEE-ELRAHLAERLPDYMVPSAFVV 427
|
490
....*....|....
gi 91684220 523 VTELPKTISGKIRR 536
Cdd:cd05930 428 LDALPLTPNGKVDR 441
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
39-542 |
4.32e-59 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 207.55 E-value: 4.32e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 39 WF-DAELAT--NADSR-------DRAALWIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWE 108
Cdd:cd05967 43 WFvGGRLNTcyNALDRhveagrgDQIALIYDSPVTGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAI 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 109 TMLAAIKLGI---VVIPAttlLTPDELRDRLDRGAAKAVVAA-----PDQVAKFADLGGDSL-------IRIVV------ 167
Cdd:cd05967 123 AMLACARIGAihsVVFGG---FAAKELASRIDDAKPKLIVTAscgiePGKVVPYKPLLDKALelsghkpHHVLVlnrpqv 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 168 -GSPQDGWRGYDEASQQSDSFTPD-GPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWL-GLQPGDVHLNIS 244
Cdd:cd05967 200 pADLTKPGRDLDWSELLAKAEPVDcVPVAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIyGIKPGDVWWAAS 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 245 SPGWA-KHAWSCFfAPWNAGATVFV-----VNQPrfDAKSLLATIGRCGVTTLCAPPTVWRMFIQE-------KLADYKv 311
Cdd:cd05967 280 DVGWVvGHSYIVY-GPLLHGATTVLyegkpVGTP--DPGAFWRVIEKYQVNALFTAPTAIRAIRKEdpdgkyiKKYDLS- 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 312 SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTET-TAMVGNSPGQ---PVKIGSMGRPLPGYVVRITDADGHPA--- 384
Cdd:cd05967 356 SLRTLFLAGERLDPPTLEWAENTLGVPVIDHWWQTETgWPITANPVGLeplPIKAGSPGKPVPGYQVQVLDEDGEPVgpn 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 385 KEGEITLALgdARPAGLMQG-YQGEGGKLSGAEGD---IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESIL 460
Cdd:cd05967 436 ELGNIVIKL--PLPPGCLLTlWKNDERFKKLYLSKfpgYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESV 513
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 461 LEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALS-IFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHL 539
Cdd:cd05967 514 LSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKeLVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTL 593
|
...
gi 91684220 540 RRL 542
Cdd:cd05967 594 RKI 596
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
70-540 |
3.59e-57 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 200.16 E-value: 3.59e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNV---VPLWetmLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVA 146
Cdd:PRK08316 38 TYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSdayALLW---LACARAGAVHVPVNFMLTGEELAYILDHSGARAFLV 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 147 APDQV----AKFADLGGDSLIRIVVGSPQ---DGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQR 219
Cdd:PRK08316 115 DPALAptaeAALALLPVDTLILSLVLGGReapGGWLDFADWAEAGSVAEPDVELADDDLAQILYTSGTESLPKGAMLTHR 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 220 SYPVGALSTMFWLGLQPGDVHLNiSSPGWAKHAWSCFFAPWNA-GATVFVVNQPrfDAKSLLATIGRCGVTTLCAPPTVW 298
Cdd:PRK08316 195 ALIAEYVSCIVAGDMSADDIPLH-ALPLYHCAQLDVFLGPYLYvGATNVILDAP--DPELILRTIEAERITSFFAPPTVW 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 299 RMFIQEKLADYK--VSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTET--TAMVGNSPGQPVKIGSMGRPLPGYV 373
Cdd:PRK08316 272 ISLLRHPDFDTRdlSSLRKGYYGASIMPVEVLKELRERLpGLRFYNCYGQTEIapLATVLGPEEHLRRPGSAGRPVLNVE 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 374 VRITDADGHPAKEGEItlalGD--ARPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYR 450
Cdd:PRK08316 352 TRVVDDDGNDVAPGEV----GEivHRSPQLMLGYWDDPEKTAEAfRGGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGEN 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 451 ISPFELESILLEHEAVAEAAVVPSPD-----AIKLAI-PKAYVLLTADAersrdtalsIFRHMQQRLAPFKRIRKLELVT 524
Cdd:PRK08316 428 VASREVEEALYTHPAVAEVAVIGLPDpkwieAVTAVVvPKAGATVTEDE---------LIAHCRARLAGFKVPKRVIFVD 498
|
490
....*....|....*.
gi 91684220 525 ELPKTISGKIRRVHLR 540
Cdd:PRK08316 499 ELPRNPSGKILKRELR 514
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
52-540 |
4.50e-57 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 199.75 E-value: 4.50e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAAlwIVDGitgvELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGN----VVPLwetmLAAIKLGIVVIPATTLL 127
Cdd:PRK07656 20 DKEA--YVFG----DQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNsphwVIAA----LGALKAGAVVVPLNTRY 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPDELRDRLDRGAAKAVVAAPDQVAKF-ADLGGDSLIRIVVGSPQDGWRGYDEASQQSDSFTPDGPT-------QADDPM 199
Cdd:PRK07656 90 TADEAAYILARGDAKALFVLGLFLGVDySATTRLPALEHVVICETEEDDPHTEKMKTFTDFLAAGDPaerapevDPDDVA 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 200 LLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLnISSPGWakHAWsCFFAPWNA----GATVFVvnQPRFD 275
Cdd:PRK07656 170 DILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRYL-AANPFF--HVF-GYKAGVNAplmrGATILP--LPVFD 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 276 AKSLLATIGRCGVTTLCAPPTVWRMFIQ-EKLADYKV-SLREVCGAGEPLNPEVIDQVNAAWG-ATIRDGYGQTE---TT 349
Cdd:PRK07656 244 PDEVFRLIETERITVLPGPPTMYNSLLQhPDRSAEDLsSLRLAVTGAASMPVALLERFESELGvDIVLTGYGLSEasgVT 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 350 AMvgNSPGQPVKI--GSMGRPLPGYVVRITDADGHPAKEGEITLALgdARPAGLMQGYQG---EGGKLSGAEGDIYrSGD 424
Cdd:PRK07656 324 TF--NRLDDDRKTvaGTIGTAIAGVENKIVNELGEEVPVGEVGELL--VRGPNVMKGYYDdpeATAAAIDADGWLH-TGD 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 425 VAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLtadAERSRDTALSIF 504
Cdd:PRK07656 399 LGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVL---KPGAELTEEELI 475
|
490 500 510
....*....|....*....|....*....|....*.
gi 91684220 505 RHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK07656 476 AYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALR 511
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
5-542 |
3.02e-55 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 197.29 E-value: 3.02e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 5 QQARAFLLEHRTDYAAAVA---GFrWPDPA-------PFNWALD-------WF-DAEL--ATN------ADSRDRAALwI 58
Cdd:PRK00174 10 ANALIDMEQYKALYQESVEdpeGF-WAEQAkrldwfkPFDTVLDwnapfikWFeDGELnvSYNcldrhlKTRGDKVAI-I 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 59 VDGITGVELKP-SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIV--VIPATtlLTPDELRDR 135
Cdd:PRK00174 88 WEGDDPGDSRKiTYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVhsVVFGG--FSAEALADR 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 136 LDRGAAKAVVAAPDQV--AKFADL---------GGDSLIRIVV----GSP------QDGWrgYDEA-SQQSDSFTPDgPT 193
Cdd:PRK00174 166 IIDAGAKLVITADEGVrgGKPIPLkanvdealaNCPSVEKVIVvrrtGGDvdwvegRDLW--WHELvAGASDECEPE-PM 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 194 QADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFW-LGLQPGDVHLNISSPGWAK-HAWSCFfAPWNAGAT--VF-- 267
Cdd:PRK00174 243 DAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYvFDYKDGDVYWCTADVGWVTgHSYIVY-GPLANGATtlMFeg 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 268 VVNQPrfDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYK----VSLREVCGAGEPLNPEvidqvnaAW-------G 336
Cdd:PRK00174 322 VPNYP--DPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKkydlSSLRLLGSVGEPINPE-------AWewyykvvG 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 337 AT---IRDGYGQTETTA-MVGNSPG-QPVKIGSMGRPLPGYVVRITDADGHPAK-EGEITLALGDARPAGLM-------- 402
Cdd:PRK00174 393 GErcpIVDTWWQTETGGiMITPLPGaTPLKPGSATRPLPGIQPAVVDEEGNPLEgGEGGNLVIKDPWPGMMRtiygdher 472
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 403 --QGYQGEggklsgaEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKL 480
Cdd:PRK00174 473 fvKTYFST-------FKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKG 545
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 91684220 481 AIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKI-RRVhLRRL 542
Cdd:PRK00174 546 QGIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKImRRI-LRKI 607
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
70-536 |
2.70e-54 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 191.76 E-value: 2.70e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV---- 145
Cdd:cd05926 16 TYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLtpkg 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 -------AAPDQVAKFADLGGDSLIRIVVgsPQDGWRGYDEASqqSDSFTPDGPTQADDPMLLYFTSGTTAKPKLV---- 214
Cdd:cd05926 96 elgpasrAASKLGLAILELALDVGVLIRA--PSAESLSNLLAD--KKNAKSEGVPLPDDLALILHTSGTTGRPKGVplth 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 215 -------RHSQRSYpvgalstmfwlGLQPGDVHLNISsPGWAKHAWSC-FFAPWNAGATVFVvnQPRFDAKSLLATIGRC 286
Cdd:cd05926 172 rnlaasaTNITNTY-----------KLTPDDRTLVVM-PLFHVHGLVAsLLSTLAAGGSVVL--PPRFSASTFWPDVRDY 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 287 GVTTLCAPPTVWRMFIQEKLADY---KVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETT-AMVGNS-PGQPVK 361
Cdd:cd05926 238 NATWYTAVPTIHQILLNRPEPNPespPPKLRFIRSCSASLPPAVLEALEATFGAPVLEAYGMTEAAhQMTSNPlPPGPRK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 362 IGSMGRPLpGYVVRITDADGH---PAKEGEITLalgdaRPAGLMQGYQG--EGGKLSGAEGDIYRSGDVAFADDDGYLTF 436
Cdd:cd05926 318 PGSVGKPV-GVEVRILDEDGEilpPGVVGEICL-----RGPNVTRGYLNnpEANAEAAFKDGWFRTGDLGYLDADGYLFL 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 437 VGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKR 516
Cdd:cd05926 392 TGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVTEE---ELRAFCRKHLAAFKV 468
|
490 500
....*....|....*....|
gi 91684220 517 IRKLELVTELPKTISGKIRR 536
Cdd:cd05926 469 PKKVYFVDELPKTATGKIQR 488
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
70-540 |
1.43e-53 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 189.73 E-value: 1.43e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAP- 148
Cdd:PRK08276 13 TYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIVSAa 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 149 --DQVAKFAD-LGGDSLIRIVVGSPQDGWRGYDEA-SQQSDSFTPDGPTQADdpmLLYfTSGTTAKPKLVRHSQRSYPVG 224
Cdd:PRK08276 93 laDTAAELAAeLPAGVPLLLVVAGPVPGFRSYEEAlAAQPDTPIADETAGAD---MLY-SSGTTGRPKGIKRPLPGLDPD 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 225 ALSTM------FWLGLQPGDVHLnisSPGWAKHAwscffAPWNAGATVF-----VVNQPRFDAKSLLATIGRCGVTTLCA 293
Cdd:PRK08276 169 EAPGMmlallgFGMYGGPDSVYL---SPAPLYHT-----APLRFGMSALalggtVVVMEKFDAEEALALIERYRVTHSQL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 294 PPTvwrMFI------QEKLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAM-VGNSPGQPVKIGSM 365
Cdd:PRK08276 241 VPT---MFVrmlklpEEVRARYDVSsLRVAIHAAAPCPVEVKRAMIDWWGPIIHEYYASSEGGGVtVITSEDWLAHPGSV 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 GRPLPGyVVRITDADGHPAKEGEItlalGD---ARPAGLMQgYQGEGGKLSGAEGDIYRS--GDVAFADDDGYLTFVGRT 440
Cdd:PRK08276 318 GKAVLG-EVRILDEDGNELPPGEI----GTvyfEMDGYPFE-YHNDPEKTAAARNPHGWVtvGDVGYLDEDGYLYLTDRK 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 441 DDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKL 520
Cdd:PRK08276 392 SDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSI 471
|
490 500
....*....|....*....|
gi 91684220 521 ELVTELPKTISGKIRRVHLR 540
Cdd:PRK08276 472 DFEDELPRTPTGKLYKRRLR 491
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
77-471 |
1.09e-51 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 182.47 E-value: 1.09e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 77 RSNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFA 155
Cdd:TIGR01733 8 RANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDSALASRLA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 156 DLGgdslIRIVVGSPQDGWRGYDEAsqqsDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSypvgALSTMFWL--- 232
Cdd:TIGR01733 88 GLV----LPVILLDPLELAALDDAP----APPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRS----LVNLLAWLarr 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 233 -GLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVV--NQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADY 309
Cdd:TIGR01733 156 yGLDPDDRVLQFASLSFDASVEE-IFGALLAGATLVVPpeDEERDDAALLAALIAEHPVTVLNLTPSLLALLAAALPPAL 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 310 KvSLREVCGAGEPLNPEVIDQVNAAWG-ATIRDGYGQTETTAMV--------GNSPGQPVKIGsmgRPLPGYVVRITDAD 380
Cdd:TIGR01733 235 A-SLRLVILGGEALTPALVDRWRARGPgARLINLYGPTETTVWStatlvdpdDAPRESPVPIG---RPLANTRLYVLDDD 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 381 GHPA---KEGEITLAlGDarpaGLMQGYQGEGGK----------LSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSS 447
Cdd:TIGR01733 311 LRPVpvgVVGELYIG-GP----GVARGYLNRPELtaerfvpdpfAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIR 385
|
410 420
....*....|....*....|....
gi 91684220 448 DYRISPFELESILLEHEAVAEAAV 471
Cdd:TIGR01733 386 GYRIELGEIEAALLRHPGVREAVV 409
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
70-536 |
2.74e-50 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 180.55 E-value: 2.74e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDR----LDRGAAKAVV 145
Cdd:cd17646 25 TYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLP----LDPGYPADRlaymLADAGPAVVL 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAPDQVAKFADLGG-DSLIRIVVGSPQDGwrgydeasqqsdsfTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRsypvG 224
Cdd:cd17646 101 TTADLAARLPAGGDvALLGDEALAAPPAT--------------PPLVPPRPDNLAYVIYTSGSTGRPKGVMVTHA----G 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 225 ALSTMFWL----GLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVfVVNQP--RFDAKSLLATIGRCGVTTLCAPPTVW 298
Cdd:cd17646 163 IVNRLLWMqdeyPLGPGDRVLQKTPLSFDVSVWE-LFWPLVAGARL-VVARPggHRDPAYLAALIREHGVTTCHFVPSML 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 299 RMFIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV------GNSPGQPVKIGsmgRPLPGY 372
Cdd:cd17646 241 RVFLAEPAAGSCASLRRVFCSGEALPPELAARFLALPGAELHNLYGPTEAAIDVthwpvrGPAETPSVPIG---RPVPNT 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 373 VVRITDADGHPAK---EGEITLAlgdarPAGLMQGYQGEGGKLS--------GAEGDIYRSGDVAFADDDGYLTFVGRTD 441
Cdd:cd17646 318 RLYVLDDALRPVPvgvPGELYLG-----GVQLARGYLGRPALTAerfvpdpfGPGSRMYRTGDLARWRPDGALEFLGRSD 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 442 DVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSifRHMQQRLAPFKRIRKLE 521
Cdd:cd17646 393 DQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAGPDTAALR--AHLAERLPEYMVPAAFV 470
|
490
....*....|....*
gi 91684220 522 LVTELPKTISGKIRR 536
Cdd:cd17646 471 VLDALPLTANGKLDR 485
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
70-540 |
6.21e-50 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 180.27 E-value: 6.21e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV---A 146
Cdd:PRK13391 26 TYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARALItsaA 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 147 APDQVAKFADLGGDSLIRIVVGSP--QDGWRGYDEA-SQQSDSFTPDGPTQADdpmLLYfTSGTTAKPKLVRHSQRSYPV 223
Cdd:PRK13391 106 KLDVARALLKQCPGVRHRLVLDGDgeLEGFVGYAEAvAGLPATPIADESLGTD---MLY-SSGTTGRPKGIKRPLPEQPP 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 G------ALSTMFWlGLQPGDVHLnisSPGWAKHAwscffAPWNA-------GATVFVVNqpRFDAKSLLATIGRCGVTT 290
Cdd:PRK13391 182 DtplpltAFLQRLW-GFRSDMVYL---SPAPLYHS-----APQRAvmlvirlGGTVIVME--HFDAEQYLALIEEYGVTH 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 291 LCAPPTvwrMFIQE-KLAD-----YKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTE---TTAMvgNSPGQPV 360
Cdd:PRK13391 251 TQLVPT---MFSRMlKLPEevrdkYDLSsLEVAIHAAAPCPPQVKEQMIDWWGPIIHEYYAATEglgFTAC--DSEEWLA 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 361 KIGSMGRPLPGyVVRITDADGHPAKEGEI-TLALGDARPAglmqGYQGEGGKLSGA---EGDIYRSGDVAFADDDGYLTF 436
Cdd:PRK13391 326 HPGTVGRAMFG-DLHILDDDGAELPPGEPgTIWFEGGRPF----EYLNDPAKTAEArhpDGTWSTVGDIGYVDEDGYLYL 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 437 VGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV--VPSPDAIKLAipKAYVLLTADAERSRDTALSIFRHMQQRLAPF 514
Cdd:PRK13391 401 TDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVfgVPNEDLGEEV--KAVVQPVDGVDPGPALAAELIAFCRQRLSRQ 478
|
490 500
....*....|....*....|....*.
gi 91684220 515 KRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK13391 479 KCPRSIDFEDELPRLPTGKLYKRLLR 504
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
70-542 |
1.12e-49 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 177.67 E-value: 1.12e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVaapd 149
Cdd:cd05935 3 TYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAV---- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivVGSPQDgwrgydeasqqsdsftpdgptqadDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05935 79 -----------------VGSELD------------------------DLALIPYTSGTTGLPKGCMHTHFSAAANALQSA 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNqpRFDAKSLLATIGRCGVTTLCA-PPTVWRMFIQEKLAD 308
Cdd:cd05935 118 VWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMA--RWDRETALELIEKYKVTFWTNiPTMLVDLLATPEFKT 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 309 YKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDA-DGH---P 383
Cdd:cd05935 196 RDLSsLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTETMSQTHTNPPLRPKLQCLGIP*FGVDARVIDIeTGRelpP 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 384 AKEGEITLalgdaRPAGLMQGY---QGEGGK----LSGAEgdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFEL 456
Cdd:cd05935 276 NEVGEIVV-----RGPQIFKGYwnrPEETEEsfieIKGRR--FFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEV 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 457 ESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTaDAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIrr 536
Cdd:cd05935 349 EAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLR-PEYRGKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKI-- 425
|
....*.
gi 91684220 537 vhLRRL 542
Cdd:cd05935 426 --LWRL 429
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
70-540 |
2.35e-49 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 177.92 E-value: 2.35e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLdrgAAKAVVAAPD 149
Cdd:cd17651 22 TYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVP----LDPAYPAERL---AFMLADAGPV 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGDSLIRIVVGSPQD--GWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSypvgALS 227
Cdd:cd17651 95 LVLTHPALAGELAVELVAVTLLDqpGAAAGADAE-------PDPALDADDLAYVIYTSGSTGRPKGVVMPHRS----LAN 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 228 TMFW----LGLQPGDVHLNISSPGWAKHAWScFFAPWNAGAT-VFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFI 302
Cdd:cd17651 164 LVAWqaraSSLGPGARTLQFAGLGFDVSVQE-IFSTLCAGATlVLPPEEVRTDPPALAAWLDEQRISRVFLPTVALRALA 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 QE--KLADYKVSLREVCGAGEPLNPEVIDQVNAAW--GATIRDGYGQTETTAM----VGNSPGQPVKIGSMGRPLPGYVV 374
Cdd:cd17651 243 EHgrPLGVRLAALRYLLTGGEQLVLTEDLREFCAGlpGLRLHNHYGPTETHVVtalsLPGDPAAWPAPPPIGRPIDNTRV 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 375 RITDADGHPA---KEGEITLAlgdarPAGLMQGYQGEGGKLS--------GAEGDIYRSGDVAFADDDGYLTFVGRTDDV 443
Cdd:cd17651 323 YVLDAALRPVppgVPGELYIG-----GAGLARGYLNRPELTAerfvpdpfVPGARMYRTGDLARWLPDGELEFLGRADDQ 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 444 FKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVllTADAERSRDTAlSIFRHMQQRLAPFKRIRKLELV 523
Cdd:cd17651 398 VKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYV--VGDPEAPVDAA-ELRAALATHLPEYMVPSAFVLL 474
|
490
....*....|....*..
gi 91684220 524 TELPKTISGKIRRVHLR 540
Cdd:cd17651 475 DALPLTPNGKLDRRALP 491
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
77-540 |
3.25e-49 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 176.86 E-value: 3.25e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 77 RSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIK----LGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVA 152
Cdd:cd05922 2 GVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYaggrLGLVFVPLNPTLKESVLRYLVADAGGRIVLADAGAAD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 153 KFADLGGDSLIRIVVGSpQDGWRGyDEASqqsdsfTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWL 232
Cdd:cd05922 82 RLRDALPASPDPGTVLD-ADGIRA-ARAS------APAHEVSHEDLALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYL 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 233 GLQPGDVHLNISSPGWAkHAWSCFFAPWNAGATVFVVNQPRFDAkSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKV- 311
Cdd:cd05922 154 GITADDRALTVLPLSYD-YGLSVLNTHLLRGATLVLTNDGVLDD-AFWEDLREHGATGLAGVPSTYAMLTRLGFDPAKLp 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 312 SLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAMVGNSPGQPV--KIGSMGRPLPGYVVRITDADGHPAKEGE 388
Cdd:cd05922 232 SLRYLTQAGGRLPQETIARLRELLpGAQVYVMYGQTEATRRMTYLPPERIleKPGSIGLAIPGGEFEILDDDGTPTPPGE 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 389 ITLALgdARPAGLMQGY----QGEGGKlsGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHE 464
Cdd:cd05922 312 PGEIV--HRGPNVMKGYwndpPYRRKE--GRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIG 387
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 465 AVAEAAVVPSPDAiklAIPKAYVLLTADAERSRDTALsifRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:cd05922 388 LIIEAAAVGLPDP---LGEKLALFVTAPDKIDPKDVL---RSLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
70-541 |
1.10e-48 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 174.46 E-value: 1.10e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDrgaakavvaapd 149
Cdd:cd05912 3 TFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLK------------ 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgyDEASQQSDSFTpdgptqaddpmlLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05912 71 ----------------------------DSDVKLDDIAT------------IMYTSGTTGKPKGVQQTFGNHWWSAIGSA 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLnISSPGWAKHAWSCFFAPWNAGATVFVVnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADY 309
Cdd:cd05912 111 LNLGLTEDDNWL-CALPLFHISGLSILMRSVIYGMTVYLV--DKFDAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGY 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 310 KVSLREVCGAGEPLNPEVIDQVnAAWGATIRDGYGQTETTA-MVGNSPGQ-PVKIGSMGRPLPGYVVRITDADGHPAKEG 387
Cdd:cd05912 188 PNNLRCILLGGGPAPKPLLEQC-KEKGIPVYQSYGMTETCSqIVTLSPEDaLNKIGSAGKPLFPVELKIEDDGQPPYEVG 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 388 EITLalgdaRPAGLMQGY--QGEGGKLSgAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEA 465
Cdd:cd05912 267 EILL-----KGPNVTKGYlnRPDATEES-FENGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPA 340
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 466 VAEAAVVPSPDAIKLAIPKAYVLLTADAersrdTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:cd05912 341 IKEAGVVGIPDDKWGQVPVAFVVSERPI-----SEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
26-543 |
2.91e-48 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 177.84 E-value: 2.91e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 26 RWPDpAPfnwALDWFdaelaTNADSRDRAALWivdgitgvelkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVP 105
Cdd:PRK07529 37 RHPD-AP---ALSFL-----LDADPLDRPETW------------TYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPE 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 106 LWETMLAAIKLGIVViPATTLLTPDELRDRLDRGAAKAVVAA--------PDQVAKF------------ADLGGD----- 160
Cdd:PRK07529 96 THFALWGGEAAGIAN-PINPLLEPEQIAELLRAAGAKVLVTLgpfpgtdiWQKVAEVlaalpelrtvveVDLARYlpgpk 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 161 -SLIRIVVGSPQDGWRGYDE--ASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPG 237
Cdd:PRK07529 175 rLAVPLIRRKAHARILDFDAelARQPGDRLFSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPG 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 238 DV--------HLNISSPgwakhawsCFFAPWNAGATVFVVNQPRFDAKSLLAT----IGRCGVTTLCAPPTVWRMFIQEK 305
Cdd:PRK07529 255 DTvfcglplfHVNALLV--------TGLAPLARGAHVVLATPQGYRGPGVIANfwkiVERYRINFLSGVPTVYAALLQVP 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 LADYKV-SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVG-NSPGQPVKIGSMGRPLPGYVVRI--TDADG 381
Cdd:PRK07529 327 VDGHDIsSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATCVSSvNPPDGERRIGSVGLRLPYQRVRVviLDDAG 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 382 H------PAKEGEITLAlgdarPAGLMQGY-QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPF 454
Cdd:PRK07529 407 RylrdcaVDEVGVLCIA-----GPNVFSGYlEAAHNKGLWLEDGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPA 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 455 ELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALS-IFRHMQQRLAPFKRIRkleLVTELPKTISGK 533
Cdd:PRK07529 482 AIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEAELLAfARDHIAERAAVPKHVR---ILDALPKTAVGK 558
|
570
....*....|
gi 91684220 534 IRRVHLRRLE 543
Cdd:PRK07529 559 IFKPALRRDA 568
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
70-540 |
1.19e-47 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 172.18 E-value: 1.19e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKaVVAAPD 149
Cdd:cd05903 3 TYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAK-VFVVPE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QvakfadlggdslirivvgspqdgWRGYDEASQqsdsftpdgptqADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05903 82 R-----------------------FRQFDPAAM------------PDAVALLLFTSGTTGEPKGVMHSHNTLSASIRQYA 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVfvVNQPRFDAKSLLATIGRCGVTTLCAPPTvwrmFIQ------ 303
Cdd:cd05903 127 ERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPV--VLQDIWDPDKALALMREHGVTFMMGATP----FLTdllnav 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 EKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPV--KIGSMGRPLPGYVVRITDADG 381
Cdd:cd05903 201 EEAGEPLSRLRTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPAPEdrRLYTDGRPLPGVEIKVVDDTG 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 382 H---PAKEGEITlalgdARPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:cd05903 281 AtlaPGVEGELL-----SRGPSVFLGYLDRPDLTADAaPEGWFRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVE 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRhmQQRLAPFKRIRKLELVTELPKTISGKIRRV 537
Cdd:cd05903 356 DLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYLD--RQGVAKQYWPERLVHVDDLPRTPSGKVQKF 433
|
...
gi 91684220 538 HLR 540
Cdd:cd05903 434 RLR 436
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
70-540 |
9.61e-47 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 171.42 E-value: 9.61e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK12406 13 SFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAHAD 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGDSLIRIVVGSPQD----------------GWRGYDEASQQSDSftPDGPTQAdDPMLLYFTSGTTAKPKL 213
Cdd:PRK12406 93 LLHGLASALPAGVTVLSVPTPPEiaaayrispalltppaGAIDWEGWLAQQEP--YDGPPVP-QPQSMIYTSGTTGHPKG 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 214 VRhsqRSYPVGALSTMFWL------GLQPGDVHLnisSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCG 287
Cdd:PRK12406 170 VR---RAAPTPEQAAAAEQmraliyGLKPGIRAL---LTGPLYHSAPNAYGLRAGRLGGVLVLQPRFDPEELLQLIERHR 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 288 VTTLCAPPTvwrMFIQ------EKLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPV 360
Cdd:PRK12406 244 ITHMHMVPT---MFIRllklpeEVRAKYDVSsLRHVIHAAAPCPADVKRAMIEWWGPVIYEYYGSTESGAVTFATSEDAL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 361 -KIGSMGRPLPGYVVRITDADGHPAKEGEItlalGD--ARPAGLMQ-GYQGEGGKLSGAE-GDIYRSGDVAFADDDGYLT 435
Cdd:PRK12406 321 sHPGTVGKAAPGAELRFVDEDGRPLPQGEI----GEiySRIAGNPDfTYHNKPEKRAEIDrGGFITSGDVGYLDADGYLF 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 436 FVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTalsIFRHMQQRLAPFK 515
Cdd:PRK12406 397 LCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGATLDEAD---IRAQLKARLAGYK 473
|
490 500
....*....|....*....|....*
gi 91684220 516 RIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK12406 474 VPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
48-540 |
1.13e-46 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 171.70 E-value: 1.13e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 48 ADSRDRAALwiVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLL 127
Cdd:PLN02246 34 SEFSDRPCL--IDGATGRVY--TYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFY 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLIRIV-VGSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSG 206
Cdd:PLN02246 110 TPAEIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVTVVtIDDPPEGCLHFSELTQADENELPEVEISPDDVVALPYSSG 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 207 TTAKPKLVRHSQRSypvgaLSTMFW---------LGLQPGDVHL------NISSPGwakhawSCFFAPWNAGATVFVVnq 271
Cdd:PLN02246 190 TTGLPKGVMLTHKG-----LVTSVAqqvdgenpnLYFHSDDVILcvlpmfHIYSLN------SVLLCGLRVGAAILIM-- 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 PRFDAKSLLATIGRCGVTTLC-APPTVWRMFIQEKLADYKV-SLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTET 348
Cdd:PLN02246 257 PKFEIGALLELIQRHKVTIAPfVPPIVLAIAKSPVVEKYDLsSIRMVLSGAAPLGKELEDAFRAKLpNAVLGQGYGMTEA 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 349 TAMVGNSPG-----QPVKIGSMGRplpgyVVR-----ITDAD-------GHPakeGEITLalgdaRPAGLMQGYQG--EG 409
Cdd:PLN02246 337 GPVLAMCLAfakepFPVKSGSCGT-----VVRnaelkIVDPEtgaslprNQP---GEICI-----RGPQIMKGYLNdpEA 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 410 GKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLL 489
Cdd:PLN02246 404 TANTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVR 483
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 91684220 490 TADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PLN02246 484 SNGSEITED---EIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLR 531
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
70-540 |
6.33e-46 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 169.55 E-value: 6.33e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK06155 48 TYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLLVVEAA 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKF--ADLGGDSL--IRIVVGSPQDGW-RGYDEAS-QQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLV--RHSQRsY 221
Cdd:PRK06155 128 LLAALeaADPGDLPLpaVWLLDAPASVSVpAGWSTAPlPPLDAPAPAAAVQPGDTAAILYTSGTTGPSKGVccPHAQF-Y 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 222 PVGALSTMFwLGLQPGDVHLNiSSPGWAKHAWSCFFAPWNAGATVFVvnQPRFDAKSLLATIGRCGVT-TLCAPPTVWRM 300
Cdd:PRK06155 207 WWGRNSAED-LEIGADDVLYT-TLPLFHTNALNAFFQALLAGATYVL--EPRFSASGFWPAVRRHGATvTYLLGAMVSIL 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 301 FIQEKLADYKVS-LREVCGAGEPlnPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPvKIGSMGRPLPGYVVRITDA 379
Cdd:PRK06155 283 LSQPARESDRAHrVRVALGPGVP--AALHAAFRERFGVDLLDGYGSTETNFVIAVTHGSQ-RPGSMGRLAPGFEARVVDE 359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DGHPAKEGEI-TLALGDARPAGLMQGYQGEGGKLSGAEGDI-YRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:PRK06155 360 HDQELPDGEPgELLLRADEPFAFATGYFGMPEKTVEAWRNLwFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVE 439
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSP-----DAIKLAI-PKAYVLLTADAersrdtalsIFRHMQQRLAPFKRIRKLELVTELPKTIS 531
Cdd:PRK06155 440 QVLLSHPAVAAAAVFPVPselgeDEVMAAVvLRDGTALEPVA---------LVRHCEPRLAYFAVPRYVEFVAALPKTEN 510
|
....*....
gi 91684220 532 GKIRRVHLR 540
Cdd:PRK06155 511 GKVQKFVLR 519
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
48-551 |
6.26e-45 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 168.05 E-value: 6.26e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 48 ADSRDRAALwIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLL 127
Cdd:cd05968 72 ADTRTRPAL-RWEGEDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGF 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPDELRDRLDRGAAKAVVAA-----------PDQVAKFADLGGDSLIRIVV---GSPQDGWRGYDEASQQSDSFTPD--- 190
Cdd:cd05968 151 GKEAAATRLQDAEAKALITAdgftrrgrevnLKEEADKACAQCPTVEKVVVvrhLGNDFTPAKGRDLSYDEEKETAGdga 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 191 GPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPV-GALSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNaGATVFVV 269
Cdd:cd05968 231 ERTESEDPLMIIYTSGTTGKPKGTVHVHAGFPLkAAQDMYFQFDLKPGDLLTWFTDLGWMMGPWLIFGGLIL-GATMVLY 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 270 N-QPRFD-AKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADY----KVSLREVCGAGEPLNPE--------VIDQ----V 331
Cdd:cd05968 310 DgAPDHPkADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVnahdLSSLRVLGSTGEPWNPEpwnwlfetVGKGrnpiI 389
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 332 NAAWGATIRDGygqtettaMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEITLALGDARPaGLMQGYQGEGGK 411
Cdd:cd05968 390 NYSGGTEISGG--------ILGNVLIKPIKPSSFNGPVPGMKADVLDESGKPARPEVGELVLLAPWP-GMTRGFWRDEDR 460
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 412 LSGAE----GDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYV 487
Cdd:cd05968 461 YLETYwsrfDNVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFV 540
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 91684220 488 LL----TADAERSRDTALSIFRHMQQRLAPfKRIRkleLVTELPKTISGKIRRVHLRRLEHDDDRADT 551
Cdd:cd05968 541 VLkpgvTPTEALAEELMERVADELGKPLSP-ERIL---FVKDLPKTRNAKVMRRVIRAAYLGKELGDL 604
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
48-542 |
1.42e-44 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 165.78 E-value: 1.42e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 48 ADSRDRAALwiVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLL 127
Cdd:cd17642 28 ASVPGTIAF--TDAHTGVNY--SYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIY 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLI--RIVVGSPQDGWRGY----------DEASQQSDSFTPDGPTQA 195
Cdd:cd17642 104 NERELDHSLNISKPTIVFCSKKGLQKVLNVQKKLKIikTIIILDSKEDYKGYqclytfitqnLPPGFNEYDFKPPSFDRD 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPKLVRHSQRSypvgaLSTMFWLGLQPgdVHLNISSPGWA-------KHAWSCFFAPWNAGATVFV 268
Cdd:cd17642 184 EQVALIMNSSGSTGLPKGVQLTHKN-----IVARFSHARDP--IFGNQIIPDTAiltvipfHHGFGMFTTLGYLICGFRV 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 269 VNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLAD-YKVS-LREVCGAGEPLNPEVIDQVNAAWGAT-IRDGYGQ 345
Cdd:cd17642 257 VLMYKFEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDkYDLSnLHEIASGGAPLSKEVGEAVAKRFKLPgIRQGYGL 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 346 TETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGH----PAKEGEITLAlGDarpaGLMQGYQG--EGGKLSGAEGDI 419
Cdd:cd17642 337 TETTSAILITPEGDDKPGAVGKVVPFFYAKVVDLDTGktlgPNERGELCVK-GP----MIMKGYVNnpEATKALIDKDGW 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 420 YRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERsrdT 499
Cdd:cd17642 412 LHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGKTM---T 488
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 91684220 500 ALSIFRHMQQRLAPFKRIR-KLELVTELPKTISGKIRRVHLRRL 542
Cdd:cd17642 489 EKEVMDYVASQVSTAKRLRgGVKFVDEVPKGLTGKIDRRKIREI 532
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
44-536 |
1.53e-44 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 166.81 E-value: 1.53e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 44 LATNADSR-DRAALWIVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIP 122
Cdd:COG1022 17 LRRRAARFpDRVALREKEDGIWQSL--TWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVP 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 123 ATTLLTPDELRDRLDRGAAKAVVAA-PDQVAKFADLGGD--SLIRIVV-----GSPQDGWRGYDEASQQSDSFTPDG--- 191
Cdd:COG1022 95 IYPTSSAEEVAYILNDSGAKVLFVEdQEQLDKLLEVRDElpSLRHIVVldprgLRDDPRLLSLDELLALGREVADPAele 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 192 ----PTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGW-AKHAWSCFFapWNAGATV 266
Cdd:COG1022 175 arraAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHvFERTVSYYA--LAAGATV 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 267 FVVNqprfDAKSLLATIGRCGVTTLCAPPTVW-------------------RMF-------------------------I 302
Cdd:COG1022 253 AFAE----SPDTLAEDLREVKPTFMLAVPRVWekvyagiqakaeeagglkrKLFrwalavgrryararlagkspslllrL 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 QEKLADYKV--SLREVCG--------AGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMV-GNSPGQpVKIGSMGRPLPG 371
Cdd:COG1022 329 KHALADKLVfsKLREALGgrlrfavsGGAALGPELARFFRAL-GIPVLEGYGLTETSPVItVNRPGD-NRIGTVGPPLPG 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 372 YVVRItdadghpAKEGEItLALGDarpaGLMQGYQG----------EGGKLsgaegdiyRSGDVAFADDDGYLTFVGRTD 441
Cdd:COG1022 407 VEVKI-------AEDGEI-LVRGP----NVMKGYYKnpeataeafdADGWL--------HTGDIGELDEDGFLRITGRKK 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 442 DVFKSSD--YrISPFELESILLEHEAVAEAAVV------------PSPDAIK-----LAIPkayvlLTADAERSRDTAL- 501
Cdd:COG1022 467 DLIVTSGgkN-VAPQPIENALKASPLIEQAVVVgdgrpflaalivPDFEALGewaeeNGLP-----YTSYAELAQDPEVr 540
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 91684220 502 ----SIFRHMQQRLAPFKRIRKLELvteLPK---------TISGKIRR 536
Cdd:COG1022 541 aliqEEVDRANAGLSRAEQIKRFRL---LPKeftiengelTPTLKLKR 585
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
70-536 |
6.55e-44 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 162.38 E-value: 6.55e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVA-AP 148
Cdd:cd05907 7 TWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVeDP 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 149 DQVAKfadlggdslirIVvgspqdgwrgydeasqqsdsftpdgptqaddpmllyFTSGTTAKPKLVRHSQRSY--PVGAL 226
Cdd:cd05907 87 DDLAT-----------II------------------------------------YTSGTTGRPKGVMLSHRNIlsNALAL 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 227 STMfwLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVnqprFDAKSLLATIGRCGVTTLCAPPTVWRMF----- 301
Cdd:cd05907 120 AER--LPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFA----SSAETLLDDLSEVRPTVFLAVPRVWEKVyaaik 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 302 ------IQEKLADYKV--SLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYV 373
Cdd:cd05907 194 vkavpgLKRKLFDLAVggRLRFAASGGAPLPAELLHFFRAL-GIPVYEGYGLTETSAVVTLNPPGDNRIGTVGKPLPGVE 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 374 VRITDadghpakEGEITLalgdaRPAGLMQGYQG--EGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFK-SSDYR 450
Cdd:cd05907 273 VRIAD-------DGEILV-----RGPNVMLGYYKnpEATAEALDADGWLHTGDLGEIDEDGFLHITGRKKDLIItSGGKN 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 451 ISPFELESILLEHEAVAEAAVV------------PSPDAIKLAIPKAYVLLTADAERSRDTAL-----SIFRHMQQRLAP 513
Cdd:cd05907 341 ISPEPIENALKASPLISQAVVIgdgrpflvalivPDPEALEAWAEEHGIAYTDVAELAANPAVraeieAAVEAANARLSR 420
|
490 500 510
....*....|....*....|....*....|
gi 91684220 514 FKRIRKLELVTElPKTI-------SGKIRR 536
Cdd:cd05907 421 YEQIKKFLLLPE-PFTIengeltpTLKLKR 449
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
70-536 |
3.40e-43 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 160.53 E-value: 3.40e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDR----LDRGAAKAVV 145
Cdd:cd12116 14 SYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVP----LDPDYPADRlryiLEDAEPALVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAPDQVAKFADLGGDSLIRivvgsPQDGWRGYDEasqqsdsftPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGA 225
Cdd:cd12116 90 TDDALPDRLPAGLPVLLLA-----LAAAAAAPAA---------PRTPVSPDDLAYVIYTSGSTGRPKGVVVSHRNLVNFL 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 226 LSTMFWLGLQPGDVHLNISSPgwakhawsCF-------FAPWNAGATVFVV-NQPRFDAKSLLATIGRCGVTTLCAPPTV 297
Cdd:cd12116 156 HSMRERLGLGPGDRLLAVTTY--------AFdisllelLLPLLAGARVVIApRETQRDPEALARLIEAHSITVMQATPAT 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 298 WRMFI---QEKLAdykvSLREVCGaGEPLNPEVIDQVnAAWGATIRDGYGQTETT-----AMVGNSPGQPVkigsMGRPL 369
Cdd:cd12116 228 WRMLLdagWQGRA----GLTALCG-GEALPPDLAARL-LSRVGSLWNLYGPTETTiwstaARVTAAAGPIP----IGRPL 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 370 PGYVVRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGE---------GGKLSGAEGDIYRSGDVAFADDDGYLTFVGR 439
Cdd:cd12116 298 ANTQVYVLDAALRPVPPGVPgELYIGGD---GVAQGYLGRpaltaerfvPDPFAGPGSRLYRTGDLVRRRADGRLEYLGR 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 440 TDDVFKSSDYRISPFELESILLEHEAVAEAAVV--PSPDAIKLAipkAYVLLTADAERSRDtALSifRHMQQRLAPFKRI 517
Cdd:cd12116 375 ADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVvrEDGGDRRLV---AYVVLKAGAAPDAA-ALR--AHLRATLPAYMVP 448
|
490
....*....|....*....
gi 91684220 518 RKLELVTELPKTISGKIRR 536
Cdd:cd12116 449 SAFVRLDALPLTANGKLDR 467
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
44-534 |
3.78e-43 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 162.05 E-value: 3.78e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 44 LATNADSR-DRAALWIVDGITgvelkpSFATMSKRSNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVI 121
Cdd:PRK08314 16 LEVSARRYpDKTAIVFYGRAI------SYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 122 PATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLIRIVV---------------------------GSPQDGW 174
Cdd:PRK08314 90 PVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRHVIvaqysdylpaepeiavpawlraepplqALAPGGV 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 175 RGYDEASQQSDsftPDGPTQA--DDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNiSSPgwakha 252
Cdd:PRK08314 170 VAWKEALAAGL---APPPHTAgpDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLA-VLP------ 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 253 wscFF----------APWNAGATVfvVNQPRFDAKSLLATIGRCGVTTLCAPPT-VWRMFIQEKLADYKV-SLREVCGAG 320
Cdd:PRK08314 240 ---LFhvtgmvhsmnAPIYAGATV--VLMPRWDREAAARLIERYRVTHWTNIPTmVVDFLASPGLAERDLsSLRYIGGGG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 321 EPLNPEVIDQVNAAWGATIRDGYGQTETTAMV-GNSPGQPvKIGSMGRPLPGYVVRITDADG----HPAKEGEITLalgd 395
Cdd:PRK08314 315 AAMPEAVAERLKELTGLDYVEGYGLTETMAQThSNPPDRP-KLQCLGIPTFGVDARVIDPETleelPPGEVGEIVV---- 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 396 aRPAGLMQGY--QGEGGKLSGAEGD---IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAA 470
Cdd:PRK08314 390 -HGPQVFKGYwnRPEATAEAFIEIDgkrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEAC 468
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 91684220 471 VVPSPDAIKLAIPKAYVLLTADAeRSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKI 534
Cdd:PRK08314 469 VIATPDPRRGETVKAVVVLRPEA-RGKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
70-536 |
1.53e-42 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 164.56 E-value: 1.53e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRL-----DRGAAkaV 144
Cdd:PRK12467 539 SYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVP----LDPEYPQDRLaymldDSGVR--L 612
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VAAPDQVAKFADLGgDSLIRIVVGSPQDGWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAKPK--LVRHSQRSYP 222
Cdd:PRK12467 613 LLTQSHLLAQLPVP-AGLRSLCLDEPADLLCGYSGHN-------PEVALDPDNLAYVIYTSGSTGQPKgvAISHGALANY 684
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 223 VGALSTmfWLGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVN-QPRFDAKSLLATIGRCGVTTLCAPPTVWRMF 301
Cdd:PRK12467 685 VCVIAE--RLQLAADDSMLMVSTFAFDLGVTE-LFGALASGATLHLLPpDCARDAEAFAALMADQGVTVLKIVPSHLQAL 761
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 302 IQEKLADYKVSLREVCGAGEPLNPEVIDQVNA-AWGATIRDGYGQTETTAMVGNSP--GQPVKIGS--MGRPLPGYVVRI 376
Cdd:PRK12467 762 LQASRVALPRPQRALVCGGEALQVDLLARVRAlGPGARLINHYGPTETTVGVSTYElsDEERDFGNvpIGQPLANLGLYI 841
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 377 TDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGG--------KLSGAEGD-IYRSGDVAFADDDGYLTFVGRTDDVFKS 446
Cdd:PRK12467 842 LDHYLNPVPVGVVgELYIGGA---GLARGYHRRPAltaerfvpDPFGADGGrLYRTGDLARYRADGVIEYLGRMDHQVKI 918
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 447 SDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIpKAYVLLTADAERSRDTALS--IFRHMQQRLAPFKRIRKLELVT 524
Cdd:PRK12467 919 RGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQL-VAYLVPAAVADGAEHQATRdeLKAQLRQVLPDYMVPAHLLLLD 997
|
490
....*....|..
gi 91684220 525 ELPKTISGKIRR 536
Cdd:PRK12467 998 SLPLTPNGKLDR 1009
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
70-540 |
5.10e-42 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 158.18 E-value: 5.10e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRVVFVDRD 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGG--DSLIRIVVGSPQD--------GWRGYDE--ASQQSDSFTPDGPtqADDPMLLYFTSGTTAKPKLVRHS 217
Cdd:cd12119 107 FLPLLEAIAPrlPTVEHVVVMTDDAampepagvGVLAYEEllAAESPEYDWPDFD--ENTAAAICYTSGTTGNPKGVVYS 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 218 QRSYPVGALSTMF--WLGLQPGDVHLNISsPGWAKHAWSCFFAPWNAGATvFVVNQPRFDAKSLLATIGRCGVTTLCAPP 295
Cdd:cd12119 185 HRSLVLHAMAALLtdGLGLSESDVVLPVV-PMFHVNAWGLPYAAAMVGAK-LVLPGPYLDPASLAELIEREGVTFAAGVP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 296 TVWRMFIQEKLADYKV--SLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTET----------TAMVGNSPGQPVKIG 363
Cdd:cd12119 263 TVWQGLLDHLEANGRDlsSLRRVVIGGSAVPRSLIEAFEER-GVRVIHAWGMTETsplgtvarppSEHSNLSEDEQLALR 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 364 SM-GRPLPGYVVRITDADGHPA-KEGEitlALGD--ARPAGLMQGY-QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVG 438
Cdd:cd12119 342 AKqGRPVPGVELRIVDDDGRELpWDGK---AVGElqVRGPWVTKSYyKNDEESEALTEDGWLRTGDVATIDEDGYLTITD 418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 439 RTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIR 518
Cdd:cd12119 419 RSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAE---ELLEFLADKVAKWWLPD 495
|
490 500
....*....|....*....|..
gi 91684220 519 KLELVTELPKTISGKIRRVHLR 540
Cdd:cd12119 496 DVVFVDEIPKTSTGKIDKKALR 517
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
70-540 |
6.13e-42 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 158.40 E-value: 6.13e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVA--- 146
Cdd:PRK07786 44 TWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSDCGAHVVVTeaa 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 147 -APDQVAKFADLGGDSLIRIVVGSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGA 225
Cdd:PRK07786 124 lAPVATAVRDIVPLLSTVVVAGGSSDDSVLGYEDLLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQA 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 226 LSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEK 305
Cdd:PRK07786 204 MTCLRTNGADINSDVGFVGVPLFHIAGIGSMLPGLLLGAPTVIYPLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQ 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 LAD-YKVSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAMVGNSPGQPV--KIGSMGRPLPGYVVRITDA-- 379
Cdd:PRK07786 284 QARpRDLALRVLSWGAAPASDTLLRQMAATFpEAQILAAFGQTEMSPVTCMLLGEDAirKLGSVGKVIPTVAARVVDEnm 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 -DGHPAKEGEITLalgdaRPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:PRK07786 364 nDVPVGEVGEIVY-----RAPTLMSGYWNNPEATAEAfAGGWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVE 438
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFrhMQQRLAPFKRIRKLELVTELPKTISGKIRRV 537
Cdd:PRK07786 439 NVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDDAALTLEDLAEF--LTDRLARYKHPKALEIVDALPRNPAGKVLKT 516
|
...
gi 91684220 538 HLR 540
Cdd:PRK07786 517 ELR 519
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
52-560 |
1.10e-41 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 161.56 E-value: 1.10e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAALwiVDGitGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDE 131
Cdd:COG1020 491 DAVAV--VFG--DQSL--TYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVP----LDPAY 560
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 132 LRDRLDR----GAAKAVVAAPDQVAKFADLGGDsliRIVVGSPqdgwrgydEASQQSDSfTPDGPTQADDPMLLYFTSGT 207
Cdd:COG1020 561 PAERLAYmledAGARLVLTQSALAARLPELGVP---VLALDAL--------ALAAEPAT-NPPVPVTPDDLAYVIYTSGS 628
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 208 TAKPKLVRHSQRsypvGALSTMFW----LGLQPGDVHLNISSPG-----WAkhawscFFAPWNAGATVFVVNQ-PRFDAK 277
Cdd:COG1020 629 TGRPKGVMVEHR----ALVNLLAWmqrrYGLGPGDRVLQFASLSfdasvWE------IFGALLSGATLVLAPPeARRDPA 698
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 278 SLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKvSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAMV---- 352
Cdd:COG1020 699 ALAELLARHRVTVLNLTPSLLRALLDAAPEALP-SLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTETTVDStyye 777
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 ---GNSPGQPVKIGsmgRPLPGYVVRITDADGHPA---KEGEITLAlGdarpAGLMQGYQG----------------EGG 410
Cdd:COG1020 778 vtpPDADGGSVPIG---RPIANTRVYVLDAHLQPVpvgVPGELYIG-G----AGLARGYLNrpeltaerfvadpfgfPGA 849
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 411 KLsgaegdiYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAiklAIPKAYVLLT 490
Cdd:COG1020 850 RL-------YRTGDLARWLPDGNLEFLGRADDQVKIRGFRIELGEIEAALLQHPGVREAVVVAREDA---PGDKRLVAYV 919
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 491 ADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDDDRADTLRGVEYREE 560
Cdd:COG1020 920 VPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLALPAPAAAAAAAAAAPPAEEEEE 989
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
70-540 |
1.14e-41 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 155.99 E-value: 1.14e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLDRgaakavvaapd 149
Cdd:cd17649 14 SYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVP----LDPEYPAERLRY----------- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadLGGDSLIRIVVGspQDGwrgydeasqqsdsftpdgptqaDDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd17649 79 -------MLEDSGAGLLLT--HHP----------------------RQLAYVIYTSGSTGTPKGVAVSHGPLAAHCQATA 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLNISSPGWAKhAWSCFFAPWNAGATVFVVNQPRF-DAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLAD 308
Cdd:cd17649 128 ERYGLTPGDRELQFASFNFDG-AHEQLLPPLICGACVVLRPDELWaSADELAEMVRELGVTVLDLPPAYLQQLAEEADRT 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 309 YK---VSLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTET--TAMVGNSPGQPVKIGS---MGRPLPGYVVRITDAD 380
Cdd:cd17649 207 GDgrpPSLRLYIFGGEALSPELLRRWLKA-PVRLFNAYGPTEAtvTPLVWKCEAGAARAGAsmpIGRPLGGRSAYILDAD 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 381 GHPAKEGEI-TLALGDArpaGLMQGYQGEGGKL--------SGAEGD-IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYR 450
Cdd:cd17649 286 LNPVPVGVTgELYIGGE---GLARGYLGRPELTaerfvpdpFGAPGSrLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFR 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 451 ISPFELESILLEHEAVAEAAVV--PSPDAIKLAipkAYVLLTADAERSRDTAlSIFRHMQQRLAPFKRIRKLELVTELPK 528
Cdd:cd17649 363 IELGEIEAALLEHPGVREAAVValDGAGGKQLV---AYVVLRAAAAQPELRA-QLRTALRASLPDYMVPAHLVFLARLPL 438
|
490
....*....|..
gi 91684220 529 TISGKIRRVHLR 540
Cdd:cd17649 439 TPNGKLDRKALP 450
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
19-541 |
1.19e-41 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 157.91 E-value: 1.19e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 19 AAAVAGFRWPDPApfnwALDWFDAELAtnaDSRDRAALWIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLL 98
Cdd:PRK13295 13 AASIAAGHWHDRT----INDDLDACVA---SCPDKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSC 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 99 LLGNvvpLWE---TMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKaVVAAP------DQVAKFADLGGD--SLIRIVV 167
Cdd:PRK13295 86 QLPN---WWEftvLYLACSRIGAVLNPLMPIFRERELSFMLKHAESK-VLVVPktfrgfDHAAMARRLRPElpALRHVVV 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 168 --GSPQDG---------WRGYDEASQQSDSFTPDgptqADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQP 236
Cdd:PRK13295 162 vgGDGADSfeallitpaWEQEPDAPAILARLRPG----PDDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGA 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 237 GDVHLnISSP-----GWAKHAwscfFAPWNAGATVfvVNQPRFDAKSLLATIGRCGVT-TLCAPPTVWRMFIQEKLADYK 310
Cdd:PRK13295 238 DDVIL-MASPmahqtGFMYGL----MMPVMLGATA--VLQDIWDPARAAELIRTEGVTfTMASTPFLTDLTRAVKESGRP 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 311 V-SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQP--VKIGSMGRPLPGYVVRITDADGHPAKEG 387
Cdd:PRK13295 311 VsSLRTFLCAGAPIPGALVERARAALGAKIVSAWGMTENGAVTLTKLDDPdeRASTTDGCPLPGVEVRVVDADGAPLPAG 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 388 EITLALgdARPAGLMQGYQG-EGGKLSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAV 466
Cdd:PRK13295 391 QIGRLQ--VRGCSNFGGYLKrPQLNGTDADG-WFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAI 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 467 AEAAVVPSPD------AIKLAIPKAYVLLTadaersrdtalsiFRHMQ-----QRLAPFKRIRKLELVTELPKTISGKIR 535
Cdd:PRK13295 468 AQVAIVAYPDerlgerACAFVVPRPGQSLD-------------FEEMVeflkaQKVAKQYIPERLVVRDALPRTPSGKIQ 534
|
....*.
gi 91684220 536 RVHLRR 541
Cdd:PRK13295 535 KFRLRE 540
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
70-540 |
1.27e-41 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 156.97 E-value: 1.27e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapD 149
Cdd:PRK06145 29 SYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYILGDAGAKLLLV--D 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QvaKFADLGGDSLIRIVVgspqdgwrgyDEASQQSDS--------FTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSY 221
Cdd:PRK06145 107 E--EFDAIVALETPKIVI----------DAAAQADSRrlaqggleIPPQAAVAPTDLVRLMYTSGTTDRPKGVMHSYGNL 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 222 PVGALSTMFWLGLQPGDVHLNIsspGWAKHAWSC----FFAPWNAGatvFVVNQPRFDAKSLLATIGRCGVT-TLCAPPT 296
Cdd:PRK06145 175 HWKSIDHVIALGLTASERLLVV---GPLYHVGAFdlpgIAVLWVGG---TLRIHREFDPEAVLAAIERHRLTcAWMAPVM 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 297 VWRMFIQEKLADYKV-SLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTET----TAMvgnSPGQPV-KIGSMGRPL 369
Cdd:PRK06145 249 LSRVLTVPDRDRFDLdSLAWCIGGGEKTPESRIRDFTRVFtRARYIDAYGLTETcsgdTLM---EAGREIeKIGSTGRAL 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 370 PGYVVRITDADGH---PAKEGEITLalgdaRPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVGRTDDVFK 445
Cdd:PRK06145 326 AHVEIRIADGAGRwlpPNMKGEICM-----RGPKVTKGYWKDPEKTAEAfYGDWFRSGDVGYLDEEGFLYLTDRKKDMII 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 446 SSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTE 525
Cdd:PRK06145 401 SGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLE---ALDRHCRQRLASFKVPRQLKVRDE 477
|
490
....*....|....*
gi 91684220 526 LPKTISGKIRRVHLR 540
Cdd:PRK06145 478 LPRNPSGKVLKRVLR 492
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
52-540 |
2.50e-41 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 156.38 E-value: 2.50e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAALwIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDE 131
Cdd:PRK08008 22 HKTAL-IFESSGGVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREE 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 132 LRDRLDRGAAKAVVAAPDQVAKFADL---GGDSLIRIVV---GSPQ-DGWRGYDEA-SQQSDSFTPDGPTQADDPMLLYF 203
Cdd:PRK08008 101 SAWILQNSQASLLVTSAQFYPMYRQIqqeDATPLRHICLtrvALPAdDGVSSFTQLkAQQPATLCYAPPLSTDDTAEILF 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 204 TSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSpgwAKHA-WSC--FFAPWNAGATVFVVNqpRFDAKSLL 280
Cdd:PRK08008 181 TSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYLTVMP---AFHIdCQCtaAMAAFSAGATFVLLE--KYSARAFW 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 281 ATIGRCGVT-TLCAPPTVWRMFIQEKLADYKV-SLREVCGAgEPLNPEVIDQVNAAWGATIRDGYGQTET-TAMVGNSPG 357
Cdd:PRK08008 256 GQVCKYRATiTECIPMMIRTLMVQPPSANDRQhCLREVMFY-LNLSDQEKDAFEERFGVRLLTSYGMTETiVGIIGDRPG 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 358 QPVKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGDARPAG-----LMQGYQG---EGGKLSGAEGDIYrSGDVAFAD 429
Cdd:PRK08008 335 DKRRWPSIGRPGFCYEAEIRDDHNRPLPAGEI----GEICIKGvpgktIFKEYYLdpkATAKVLEADGWLH-TGDTGYVD 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 430 DDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTalsIFRHMQQ 509
Cdd:PRK08008 410 EEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEEE---FFAFCEQ 486
|
490 500 510
....*....|....*....|....*....|.
gi 91684220 510 RLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK08008 487 NMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
41-541 |
4.12e-41 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 157.42 E-value: 4.12e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 41 DAELATNADSRdraALWIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGI-- 118
Cdd:PRK10524 60 DRHLAKRPEQL---ALIAVSTETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAih 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 119 -VVI---PATTLLTpdelrdRLDRGAAKAVVAApDQVAKfadlGG---------DSLIRIVVGSP--------------- 170
Cdd:PRK10524 137 sVVFggfASHSLAA------RIDDAKPVLIVSA-DAGSR----GGkvvpykpllDEAIALAQHKPrhvllvdrglapmar 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 171 QDGwRGYDEASQQSDSFTPDGPTQ---ADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWL-GLQPGDVHLNISSP 246
Cdd:PRK10524 206 VAG-RDVDYATLRAQHLGARVPVEwleSNEPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIfGGKAGETFFCASDI 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 247 GWA-KHAWsCFFAPWNAG-ATVFVVNQP-RFDAKSLLATIGRCGVTTLCAPPTVWRM---FIQEKLADYKVS-LREVCGA 319
Cdd:PRK10524 285 GWVvGHSY-IVYAPLLAGmATIMYEGLPtRPDAGIWWRIVEKYKVNRMFSAPTAIRVlkkQDPALLRKHDLSsLRALFLA 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 320 GEPLnpeviDQVNAAW-----GATIRDGYGQTETT-AMVGNSPG---QPVKIGSMGRPLPGYVVRITD-ADGHPAKEGEI 389
Cdd:PRK10524 364 GEPL-----DEPTASWisealGVPVIDNYWQTETGwPILAIARGvedRPTRLGSPGVPMYGYNVKLLNeVTGEPCGPNEK 438
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 390 -TLALGDARPAGLMQGYQGEGGKL-----SGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEH 463
Cdd:PRK10524 439 gVLVIEGPLPPGCMQTVWGDDDRFvktywSLFGRQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSH 518
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 464 EAVAEAAVVPSPDAIKLAIPKAYVLL-----TADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIrrvh 538
Cdd:PRK10524 519 PAVAEVAVVGVKDALKGQVAVAFVVPkdsdsLADREARLALEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKL---- 594
|
...
gi 91684220 539 LRR 541
Cdd:PRK10524 595 LRR 597
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
40-542 |
5.90e-41 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 155.69 E-value: 5.90e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 40 FDAELATNADSR-DRAALwiVDGitgvELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGi 118
Cdd:COG1021 27 LGDLLRRRAERHpDRIAV--VDG----ERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAG- 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 119 vVIPATTLltPD----ELRDRLDRGAAKAVVaAPDQVAKFADlggDSLIR------------IVVGSPQDgWRGYDEASQ 182
Cdd:COG1021 100 -AIPVFAL--PAhrraEISHFAEQSEAVAYI-IPDRHRGFDY---RALARelqaevpslrhvLVVGDAGE-FTSLDALLA 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 183 QSDSFTPDGPtQADDPMLLYFTSGTTAKPKLV--RHSQRSYPVGALSTMfwLGLQPGDVHL-------N--ISSPGwakh 251
Cdd:COG1021 172 APADLSEPRP-DPDDVAFFQLSGGTTGLPKLIprTHDDYLYSVRASAEI--CGLDADTVYLaalpaahNfpLSSPG---- 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 252 awscFFAPWNAGATvfVVNQPRFDAKSLLATIGRCGVT-TLCAPPTVWRMFIQEKLADYKV-SLREVCGAGEPLNPEVID 329
Cdd:COG1021 245 ----VLGVLYAGGT--VVLAPDPSPDTAFPLIERERVTvTALVPPLALLWLDAAERSRYDLsSLRVLQVGGAKLSPELAR 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 330 QVNAAWGATIRDGYGQTE----TTamvgnSPGQP--VKIGSMGRPL-PGYVVRITDADGHPAKEGEITLALgdARPAGLM 402
Cdd:COG1021 319 RVRPALGCTLQQVFGMAEglvnYT-----RLDDPeeVILTTQGRPIsPDDEVRIVDEDGNPVPPGEVGELL--TRGPYTI 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 403 QGYQG--EGGKLS-GAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIK 479
Cdd:COG1021 392 RGYYRapEHNARAfTPDG-FYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYL 470
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 91684220 480 LAIPKAYVLLTADAErsrdTALSIFRHMQQR-LAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:COG1021 471 GERSCAFVVPRGEPL----TLAELRRFLRERgLAAFKLPDRLEFVDALPLTAVGKIDKKALRAA 530
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
70-547 |
1.33e-40 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 154.70 E-value: 1.33e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV---- 145
Cdd:PRK07788 76 TYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLAEVAAREGVKALVydde 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 ------AAPDQVAKFADLGGDsliRIVVGSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLyfTSGTTAKPKLVRHSQR 219
Cdd:PRK07788 156 ftdllsALPPDLGRLRAWGGN---PDDDEPSGSTDETLDDLIAGSSTAPLPKPPKPGGIVIL--TSGTTGTPKGAPRPEP 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 220 S--YPVGALSTmfWLGLQPGDVHLnISSPgwAKHAW--SCFFAPWNAGATvfVVNQPRFDAKSLLATIGRCGVTTLCAPP 295
Cdd:PRK07788 231 SplAPLAGLLS--RVPFRAGETTL-LPAP--MFHATgwAHLTLAMALGST--VVLRRRFDPEATLEDIAKHKATALVVVP 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 296 T-VWRMF--IQEKLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPgQPVKI--GSMGRPL 369
Cdd:PRK07788 304 VmLSRILdlGPEVLAKYDTSsLKIIFVSGSALSPELATRALEAFGPVLYNLYGSTEVAFATIATP-EDLAEapGTVGRPP 382
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 370 PGYVVRITDADGHPAKEGEI-TLALGDARPaglMQGYQGEGGKLSgaEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSD 448
Cdd:PRK07788 383 KGVTVKILDENGNEVPRGVVgRIFVGNGFP---FEGYTDGRDKQI--IDGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGG 457
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 449 YRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPK 528
Cdd:PRK07788 458 ENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAALDED---AIKDYVRDNLARYKVPRDVVFLDELPR 534
|
490
....*....|....*....
gi 91684220 529 TISGKIrrvhLRRLEHDDD 547
Cdd:PRK07788 535 NPTGKV----LKRELREMD 549
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
70-543 |
1.35e-40 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 153.58 E-value: 1.35e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK03640 29 TFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQLDDAEVKCLITDDD 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakFADlggdsliRIVVGSPQDgwrgYDEASQQSDS-FTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALST 228
Cdd:PRK03640 109 ----FEA-------KLIPGISVK----FAELMNGPKEeAEIQEEFDLDEVATIMYTSGTTGKPKGVIQTYGNHWWSAVGS 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 229 MFWLGLQPGDVHLnISSPGWAKHAWSCFFAPWNAGATVFVVnqPRFDAKSLLATIGRCGVTTLCAPPT-VWRMFIQEKLA 307
Cdd:PRK03640 174 ALNLGLTEDDCWL-AAVPIFHISGLSILMRSVIYGMRVVLV--EKFDAEKINKLLQTGGVTIISVVSTmLQRLLERLGEG 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 308 DYKVSLREVCGAGEPLNPEVIDQVnAAWGATIRDGYGQTETTA-MVGNSPGQPV-KIGSMGRPLPGYVVRITD--ADGHP 383
Cdd:PRK03640 251 TYPSSFRCMLLGGGPAPKPLLEQC-KEKGIPVYQSYGMTETASqIVTLSPEDALtKLGSAGKPLFPCELKIEKdgVVVPP 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 384 AKEGEITLalgdaRPAGLMQGY--QGEGGKLSGAEGDIYrSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILL 461
Cdd:PRK03640 330 FEEGEIVV-----KGPNVTKGYlnREDATRETFQDGWFK-TGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLL 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 462 EHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAersrdTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:PRK03640 404 SHPGVAEAGVVGVPDDKWGQVPVAFVVKSGEV-----TEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQ 478
|
..
gi 91684220 542 LE 543
Cdd:PRK03640 479 LV 480
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
70-542 |
2.75e-40 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 152.74 E-value: 2.75e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPd 149
Cdd:cd12117 24 TYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPAERLAFMLADAGAKVLLTDR- 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvaKFADLGGDSLIRIVVgspQDGWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd12117 103 ---SLAGRAGGLEVAVVI---DEALDAGPAGN-------PAVPVSPDDLAYVMYTSGSTGRPKGVAVTHRGVVRLVKNTN 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 fWLGLQPGDVHLNISSPGW-AkhawSCF--FAPWNAGATVfVVNQPRF--DAKSLLATIGRCGVTTLCAPPTVWRMFIQE 304
Cdd:cd12117 170 -YVTLGPDDRVLQTSPLAFdA----STFeiWGALLNGARL-VLAPKGTllDPDALGALIAEEGVTVLWLTAALFNQLADE 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 305 KlADYKVSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAMV-------GNSPGQPVKIGsmgRPLPGYVVRI 376
Cdd:cd12117 244 D-PECFAGLRELLTGGEVVSPPHVRRVLAACpGLRLVNGYGPTENTTFTtshvvteLDEVAGSIPIG---RPIANTRVYV 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 377 TDADGHPAKEG---EITLAlGDarpaGLMQGYQG---------------EGGKLsgaegdiYRSGDVAFADDDGYLTFVG 438
Cdd:cd12117 320 LDEDGRPVPPGvpgELYVG-GD----GLALGYLNrpaltaerfvadpfgPGERL-------YRTGDLARWLPDGRLEFLG 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 439 RTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadAERSRDTAlSIFRHMQQRLAPFKRIR 518
Cdd:cd12117 388 RIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYVV----AEGALDAA-ELRAFLRERLPAYMVPA 462
|
490 500
....*....|....*....|....
gi 91684220 519 KLELVTELPKTISGKIRRvhlRRL 542
Cdd:cd12117 463 AFVVLDELPLTANGKVDR---RAL 483
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
191-541 |
6.95e-40 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 150.90 E-value: 6.95e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 191 GPTQADDPMLLYFTSGTTAKPKLVRHSQRSypVGALSTMF---WlGLQPGDVHLNISsPgwAKHAWSCFFA---PWNAGA 264
Cdd:cd05941 84 EPSLVLDPALILYTSGTTGRPKGVVLTHAN--LAANVRALvdaW-RWTEDDVLLHVL-P--LHHVHGLVNAllcPLFAGA 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 265 TVfvVNQPRFDAKSLLATIGRCGVTTLCAPPTVW--------RMF--IQEKLADYKVSLR-EVCGAGePLNPEVIDQVNA 333
Cdd:cd05941 158 SV--EFLPKFDPKEVAISRLMPSITVFMGVPTIYtrllqyyeAHFtdPQFARAAAAERLRlMVSGSA-ALPVPTLEEWEA 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 334 AWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPA----KEGEITLalgdaRPAGLMQGY--QG 407
Cdd:cd05941 235 ITGHTLLERYGMTEIGMALSNPLDGERRPGTVGMPLPGVQARIVDEETGEPlprgEVGEIQV-----RGPSVFKEYwnKP 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 408 EGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRT-DDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAY 486
Cdd:cd05941 310 EATKEEFTDDGWFKTGDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAV 389
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 91684220 487 VLLTADAErsrdtALSI--FRHM-QQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:cd05941 390 VVLRAGAA-----ALSLeeLKEWaKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
43-542 |
2.33e-39 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 150.35 E-value: 2.33e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 43 ELATNADSRDRAALWIVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIP 122
Cdd:cd05923 5 EMLRRAASRAPDACAIADPARGLRL--TYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPAL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 123 ATTLLTPDELRDRLDRGAAKAVVAAPDqvAKFADLGGDSLIRIVVGSPQDGWRgydEASQQSDSFtPDGPTQADDPMLLY 202
Cdd:cd05923 83 INPRLKAAELAELIERGEMTAAVIAVD--AQVMDAIFQSGVRVLALSDLVGLG---EPESAGPLI-EDPPREPEQPAFVF 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 203 FTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDvHLNISSPGWAKHA---WSCFFAPWNAGATVFVVNQprFDAKSL 279
Cdd:cd05923 157 YTSGTTGLPKGAVIPQRAAESRVLFMSTQAGLRHGR-HNVVLGLMPLYHVigfFAVLVAALALDGTYVVVEE--FDPADA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 280 LATIGRCGVTTLCAPPTVWRMFI-QEKLADYKV-SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNspg 357
Cdd:cd05923 234 LKLIEQERVTSLFATPTHLDALAaAAEFAGLKLsSLRHVTFAGATMPDAVLERVNQHLPGEKVNIYGTTEAMNSLYM--- 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 358 QPVKIGSMGRPlpGY-----VVRITDADGH---PAKEGEITLAL-GDARPAGLMQGYQGEGGKLSgaEGdIYRSGDVAFA 428
Cdd:cd05923 311 RDARTGTEMRP--GFfsevrIVRIGGSPDEalaNGEEGELIVAAaADAAFTGYLNQPEATAKKLQ--DG-WYRTGDVGYV 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 429 DDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVL-----LTADA--ERSRDTAl 501
Cdd:cd05923 386 DPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVpregtLSADEldQFCRASE- 464
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 91684220 502 sifrhmqqrLAPFKRIRKLELVTELPKTISGKIrrvhLRRL 542
Cdd:cd05923 465 ---------LADFKRPRRYFFLDELPKNAMNKV----LRRQ 492
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
70-536 |
5.08e-39 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 148.62 E-value: 5.08e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLdrgaakAVVAApd 149
Cdd:cd12115 26 TYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVP----LDPAYPPERL------RFILE-- 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggDSLIRIVVgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSypvgALSTM 229
Cdd:cd12115 94 ----------DAQARLVL-------------------------TDPDDLAYVIYTSGSTGRPKGVAIEHRN----AAAFL 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLqpgdvhlNISSPGWAKHAWS---CF-------FAPWNAGATVFVVNqprfDAKSLLATIGRCGVTTLCAPPTVWR 299
Cdd:cd12115 135 QWAAA-------AFSAEELAGVLAStsiCFdlsvfelFGPLATGGKVVLAD----NVLALPDLPAAAEVTLINTVPSAAA 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 300 MFIQekLADYKVSLREVCGAGEPLNPEVIDQVNAAWGAT-IRDGYGQTETT-----AMVGNSPGQPVKIGsmgRPLPGYV 373
Cdd:cd12115 204 ELLR--HDALPASVRVVNLAGEPLPRDLVQRLYARLQVErVVNLYGPSEDTtystvAPVPPGASGEVSIG---RPLANTQ 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 374 VRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGGKLS--------GAEGDIYRSGDVAFADDDGYLTFVGRTDDVF 444
Cdd:cd12115 279 AYVLDRALQPVPLGVPgELYIGGA---GVARGYLGRPGLTAerflpdpfGPGARLYRTGDLVRWRPDGLLEFLGRADNQV 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 445 KSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVT 524
Cdd:cd12115 356 KVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAGLVE---DLRRHLGTRLPAYMVPSRFVRLD 432
|
490
....*....|..
gi 91684220 525 ELPKTISGKIRR 536
Cdd:cd12115 433 ALPLTPNGKIDR 444
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
70-540 |
5.25e-39 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 150.19 E-value: 5.25e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAA-- 147
Cdd:PRK07470 34 TWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPDEVAYLAEASGARAMICHad 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 148 -PDQVAKFADLGGDSLIRIVVGSPqDGWRGYDEA-SQQSDSFTPDGPTQADDPMLLYFTSGTTAKPK--LVRHSQRSYPV 223
Cdd:PRK07470 114 fPEHAAAVRAASPDLTHVVAIGGA-RAGLDYEALvARHLGARVANAAVDHDDPCWFFFTSGTTGRPKaaVLTHGQMAFVI 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 galsTMFWLGLQPGDVHLNIS------SPGWAKHAWsCFFApwnAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTV 297
Cdd:PRK07470 193 ----TNHLADLMPGTTEQDASlvvaplSHGAGIHQL-CQVA---RGAATVLLPSERFDPAEVWALVERHRVTNLFTVPTI 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 298 WRMFIQEKLADY--KVSLREVCGAGEPLNPEviDQVNA--AWGATIRDGYGQTETT--------AMVGNSPGQPVKIGSM 365
Cdd:PRK07470 265 LKMLVEHPAVDRydHSSLRYVIYAGAPMYRA--DQKRAlaKLGKVLVQYFGLGEVTgnitvlppALHDAEDGPDARIGTC 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 GRPLPGYVVRITDADGH---PAKEGEITLAlGDARPAGLmqgYQGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDD 442
Cdd:PRK07470 343 GFERTGMEVQIQDDEGRelpPGETGEICVI-GPAVFAGY---YNNPEANAKAFRDGWFRTGDLGHLDARGFLYITGRASD 418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 443 VFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSifrHMQQRLAPFKRIRKLEL 522
Cdd:PRK07470 419 MYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEAELLA---WLDGKVARYKLPKRFFF 495
|
490
....*....|....*...
gi 91684220 523 VTELPKTISGKIRRVHLR 540
Cdd:PRK07470 496 WDALPKSGYGKITKKMVR 513
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
62-545 |
8.96e-39 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 148.85 E-value: 8.96e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 62 ITGVELKPSFATMSKRSNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGA 140
Cdd:PRK06839 21 IITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSG 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 141 AKAVVAAPDQVAKFADLGGDSLIRIVVgspqdgW-RGYDEASQQS-DSFTPDGptqADDPMLLYFTSGTTAKPKLVRHSQ 218
Cdd:PRK06839 101 TTVLFVEKTFQNMALSMQKVSYVQRVI------SiTSLKEIEDRKiDNFVEKN---ESASFIICYTSGTTGKPKGAVLTQ 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 219 RSYPVGALSTMFWLGLQPGDVHLNISsPGWAKHAWSCF-FAPWNAGATVFVVNqpRFDAKSLLATIGRCGVTTLCAPPTV 297
Cdd:PRK06839 172 ENMFWNALNNTFAIDLTMHDRSIVLL-PLFHIGGIGLFaFPTLFAGGVIIVPR--KFEPTKALSMIEKHKVTVVMGVPTI 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 298 WRMFIQ--EKLADYKVSLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTET--TAMVGNSPGQPVKIGSMGRPLPGYV 373
Cdd:PRK06839 249 HQALINcsKFETTNLQSVRWFYNGGAPCPEELMREFIDR-GFLFGQGFGMTETspTVFMLSEEDARRKVGSIGKPVLFCD 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 374 VRITDADGHPAKEGEITLALgdARPAGLMQGY-QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRIS 452
Cdd:PRK06839 328 YELIDENKNKVEVGEVGELL--IRGPNVMKEYwNRPDATEETIQDGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIY 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 453 PFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERsrdTALSIFRHMQQRLAPFKRIRKLELVTELPKTISG 532
Cdd:PRK06839 406 PLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVL---IEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATG 482
|
490
....*....|...
gi 91684220 533 KIRRVHLRRLEHD 545
Cdd:PRK06839 483 KIQKAQLVNQLKS 495
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
70-536 |
1.79e-38 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 147.01 E-value: 1.79e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:cd05945 18 TYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIREILDAAKPALLIADGD 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAkfadlggdslirivvgspqdgwrgYdeasqqsdsftpdgptqaddpmlLYFTSGTTAKPKLVRHSQR---SYPVGAL 226
Cdd:cd05945 98 DNA------------------------Y-----------------------IIFTSGSTGRPKGVQISHDnlvSFTNWML 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 227 STmfwLGLQPGDVHLNisspgwakHAWSCF-------FAPWNAGATVFVVnqPR---FDAKSLLATIGRCGVTTLCAPPT 296
Cdd:cd05945 131 SD---FPLGPGDVFLN--------QAPFSFdlsvmdlYPALASGATLVPV--PRdatADPKQLFRFLAEHGITVWVSTPS 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 297 VWRM------FIQEKLAdykvSLREVCGAGEPL-NPEVIDQVNAAWGATIRDGYGQTETTAMV-GNSPGQPVKIGS---- 364
Cdd:cd05945 198 FAAMcllsptFTPESLP----SLRHFLFCGEVLpHKTARALQQRFPDARIYNTYGPTEATVAVtYIEVTPEVLDGYdrlp 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 365 MGRPLPGYVVRITDADGHPAKE---GEITLAlGDArpagLMQGYQGEGGK----LSGAEGD-IYRSGDVAFADDDGYLTF 436
Cdd:cd05945 274 IGYAKPGAKLVILDEDGRPVPPgekGELVIS-GPS----VSKGYLNNPEKtaaaFFPDEGQrAYRTGDLVRLEADGLLFY 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 437 VGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSifRHMQQRLAPFKR 516
Cdd:cd05945 349 RGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEAGLTKAIK--AELAERLPPYMI 426
|
490 500
....*....|....*....|
gi 91684220 517 IRKLELVTELPKTISGKIRR 536
Cdd:cd05945 427 PRRFVYLDELPLNANGKIDR 446
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
52-540 |
6.75e-38 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 146.31 E-value: 6.75e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAALWIVDgiTGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGN----VVPLWetmlAAIKLGIVVIPATTLL 127
Cdd:PRK13390 12 DRPAVIVAE--TGEQV--SYRQLDDDSAALARVLYDAGLRTGDVVALLSDNspeaLVVLW----AALRSGLYITAINHHL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLIRIVVGSPQDGWRGYDEAsqqsdsFTPDGPTQADDP--MLLYFTS 205
Cdd:PRK13390 84 TAPEADYIVGDSGARVLVASAALDGLAAKVGADLPLRLSFGGEIDGFGSFEAA------LAGAGPRLTEQPcgAVMLYSS 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 206 GTTAKPKLVRH-------SQRSYPVGALSTMFWlGLQPGDVHLNiSSPGWakHAwscffAP--WNA-----GATVFVVNq 271
Cdd:PRK13390 158 GTTGFPKGIQPdlpgrdvDAPGDPIVAIARAFY-DISESDIYYS-SAPIY--HA-----APlrWCSmvhalGGTVVLAK- 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 pRFDAKSLLATIGRCGVTTLCAPPTvwrMFIQE-KLAD-----YKV-SLREVCGAGEPLnPEVIDQVNAAW-GATIRDGY 343
Cdd:PRK13390 228 -RFDAQATLGHVERYRITVTQMVPT---MFVRLlKLDAdvrtrYDVsSLRAVIHAAAPC-PVDVKHAMIDWlGPIVYEYY 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 344 GQTETTAM-VGNSPGQPVKIGSMGRPLPGyVVRITDADGHPAKEGEITLAL--GDARPAGLMQGYQGEGGKLSGAEGDIY 420
Cdd:PRK13390 303 SSTEAHGMtFIDSPDWLAHPGSVGRSVLG-DLHICDDDGNELPAGRIGTVYfeRDRLPFRYLNDPEKTAAAQHPAHPFWT 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 421 RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTA 500
Cdd:PRK13390 382 TVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELA 461
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 91684220 501 LSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK13390 462 RELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
70-539 |
1.17e-37 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 144.76 E-value: 1.17e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlltpdelrdrLDRGAAKAVVAApd 149
Cdd:cd17643 14 TYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVP-------------IDPAYPVERIAF-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadLGGDSLIRIVVgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPK--LVRHSQrsypVGAL- 226
Cdd:cd17643 79 -------ILADSGPSLLL-------------------------TDPDDLAYVIYTSGSTGRPKgvVVSHAN----VLALf 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 227 -STMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEK 305
Cdd:cd17643 123 aATQRWFGFNEDDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPYEVARSPEDFARLLRDEGVTVLNQTPSAFYQLVEAA 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 LADYK--VSLREVCGAGEPLNPEVIDQVNAAWG---ATIRDGYGQTETTAMVG-----NSPGQPVKIGSMGRPLPGYVVR 375
Cdd:cd17643 203 DRDGRdpLALRYVIFGGEALEAAMLRPWAGRFGldrPQLVNMYGITETTVHVTfrpldAADLPAAAASPIGRPLPGLRVY 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 376 ITDADGHPAK---EGEITLAlgdarPAGLMQGYQGEGGKLS--------GAEGD-IYRSGDVAFADDDGYLTFVGRTDDV 443
Cdd:cd17643 283 VLDADGRPVPpgvVGELYVS-----GAGVARGYLGRPELTAerfvanpfGGPGSrMYRTGDLARRLPDGELEYLGRADEQ 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 444 FKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVlltADAERSRDTALSIFRHMQQRLAPFKRIRKLELV 523
Cdd:cd17643 358 VKIRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYV---VADDGAAADIAELRALLKELLPDYMVPARYVPL 434
|
490
....*....|....*.
gi 91684220 524 TELPKTISGKIRRVHL 539
Cdd:cd17643 435 DALPLTVNGKLDRAAL 450
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
77-540 |
2.76e-37 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 144.36 E-value: 2.76e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 77 RSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapDQVAKFAD 156
Cdd:cd12118 38 RCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFILRHSEAKVLFV--DREFEYED 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 157 LggdslirIVVGSPqdgwrgydeasqqsdSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQP 236
Cdd:cd12118 116 L-------LAEGDP---------------DFEWIPPADEWDPIALNYTSGTTGRPKGVVYHHRGAYLNALANILEWEMKQ 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 237 GDV--------HLNisspGWakhawsCFfaPW----NAGATVFVvnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMfiqe 304
Cdd:cd12118 174 HPVylwtlpmfHCN----GW------CF--PWtvaaVGGTNVCL---RKVDAKAIYDLIEKHKVTHFCGAPTVLNM---- 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 305 kLADYKVSLRE-------VCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMVGNSPGQP--------VKIGSMGRPL 369
Cdd:cd12118 235 -LANAPPSDARplphrvhVMTAGAPPPAAVLAKMEEL-GFDVTHVYGLTETYGPATVCAWKPewdelpteERARLKARQG 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 370 PGYV----VRITDADG-----HPAKE-GEITLalgdaRPAGLMQGYQGEGGKLSGA-EGDIYRSGDVAFADDDGYLTFVG 438
Cdd:cd12118 313 VRYVgleeVDVLDPETmkpvpRDGKTiGEIVF-----RGNIVMKGYLKNPEATAEAfRGGWFHSGDLAVIHPDGYIEIKD 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 439 RTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERsrdTALSIFRHMQQRLAPFKRIR 518
Cdd:cd12118 388 RSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKV---TEEEIIAFCREHLAGFMVPK 464
|
490 500
....*....|....*....|..
gi 91684220 519 KLELVtELPKTISGKIRRVHLR 540
Cdd:cd12118 465 TVVFG-ELPKTSTGKIQKFVLR 485
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
86-542 |
4.61e-37 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 144.98 E-value: 4.61e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 86 RAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDsliri 165
Cdd:PLN02574 85 HVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTSPENVEKLSPLGVP----- 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 166 VVGSPQDGwrGYDEASQQSDSFT----------PDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYpvgaLSTM-----F 230
Cdd:PLN02574 160 VIGVPENY--DFDSKRIEFPKFYelikedfdfvPKPVIKQDDVAAIMYSSGTTGASKGVVLTHRNL----IAMVelfvrF 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 231 WLGL--QPG--DVHLNISsPGWAKHAWSCFFAPW-NAGATVFVVNqpRFDAKSLLATIGRCGVTTL-CAPPTVWRMFIQE 304
Cdd:PLN02574 234 EASQyeYPGsdNVYLAAL-PMFHIYGLSLFVVGLlSLGSTIVVMR--RFDASDMVKVIDRFKVTHFpVVPPILMALTKKA 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 305 KLADYKV--SLREVCGAGEPLNPEVIDQ-VNAAWGATIRDGYGQTETTAM--VGNSPGQPVKIGSMGRPLPGYVVRITDA 379
Cdd:PLN02574 311 KGVCGEVlkSLKQVSCGAAPLSGKFIQDfVQTLPHVDFIQGYGMTESTAVgtRGFNTEKLSKYSSVGLLAPNMQAKVVDW 390
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DGH----PAKEGEITLalgdaRPAGLMQGY--QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISP 453
Cdd:PLN02574 391 STGcllpPGNCGELWI-----QGPGVMKGYlnNPKATQSTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAP 465
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 454 FELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadaeRSRDTALS---IFRHMQQRLAPFKRIRKLELVTELPKTI 530
Cdd:PLN02574 466 ADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVV------RRQGSTLSqeaVINYVAKQVAPYKKVRKVVFVQSIPKSP 539
|
490
....*....|..
gi 91684220 531 SGKIRRVHLRRL 542
Cdd:PLN02574 540 AGKILRRELKRS 551
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
196-540 |
8.11e-37 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 140.11 E-value: 8.11e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVhLNISSPGWakHAWSCFFAPWNA---GATVfVVNQP 272
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDR-LCIPVPLF--HCFGSVLGVLAClthGATM-VFPSP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 273 RFDAKSLLATIGRCGVTTLCAPPTvwrMFIQE----KLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGAT-IRDGYGQT 346
Cdd:cd05917 78 SFDPLAVLEAIEKEKCTALHGVPT---MFIAElehpDFDKFDLSsLRTGIMAGAPCPPELMKRVIEVMNMKdVTIAYGMT 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 347 ETTAMVGNS---PGQPVKIGSMGRPLPGYVVRITDADG----HPAKEGEITLalgdaRPAGLMQGYQGEGGKLSGAE-GD 418
Cdd:cd05917 155 ETSPVSTQTrtdDSIEKRVNTVGRIMPHTEAKIVDPEGgivpPVGVPGELCI-----RGYSVMKGYWNDPEKTAEAIdGD 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 419 I-YRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSR 497
Cdd:cd05917 230 GwLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGAELTE 309
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 91684220 498 DTalsIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:cd05917 310 ED---IKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
70-539 |
1.23e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 143.64 E-value: 1.23e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAApD 149
Cdd:PRK06178 60 TYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAEVLLAL-D 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFAD--LGGDSLIRIVVGSPQD----------------------GWRGYDEASQQSDSFTPDGPTQADDPMLLYFTS 205
Cdd:PRK06178 139 QLAPVVEqvRAETSLRHVIVTSLADvlpaeptlplpdslraprlaaaGAIDLLPALRACTAPVPLPPPALDALAALNYTG 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 206 GTTAKPKLVRHSQR--------SYPVGalstmfwLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNqpRFDAK 277
Cdd:PRK06178 219 GTTGMPKGCEHTQRdmvytaaaAYAVA-------VVGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLVLLA--RWDAV 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 278 SLLATIGRCGVTTLCAP-PTVWRMFIQEKLADYKV-SLREVCGAG--EPLNPEVIDQVNAAWGATIRDG-YGQTET---- 348
Cdd:PRK06178 290 AFMAAVERYRVTRTVMLvDNAVELMDHPRFAEYDLsSLRQVRVVSfvKKLNPDYRQRWRALTGSVLAEAaWGMTEThtcd 369
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 349 TAMVG------NSPGQPVKIGsmgRPLPGYVVRITDAD-GHP---AKEGEITLalgdaRPAGLMQGYQGEGGKLSGA-EG 417
Cdd:PRK06178 370 TFTAGfqdddfDLLSQPVFVG---LPVPGTEFKICDFEtGELlplGAEGEIVV-----RTPSLLKGYWNKPEATAEAlRD 441
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 418 DIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSR 497
Cdd:PRK06178 442 GWLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTA 521
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 91684220 498 DTALSIFRhmqQRLAPFKrIRKLELVTELPKTISGKIRRVHL 539
Cdd:PRK06178 522 AALQAWCR---ENMAVYK-VPEIRIVDALPMTATGKVRKQDL 559
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
42-541 |
1.52e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 142.90 E-value: 1.52e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 42 AELATNADSRDRAALWIVDGITgvelkpSFATMSKRSNQVANYLRAQgLKRGD--HLLLLLGNVvPLWETMLAAIKL-GI 118
Cdd:PRK07867 8 AELLLPLAEDDDRGLYFEDSFT------SWREHIRGSAARAAALRAR-LDPTRppHVGVLLDNT-PEFSLLLGAAALsGI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 119 VVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLIrIVVGSPQdgWRgyDEASQQSDSFTPDGPTQADDP 198
Cdd:PRK07867 80 VPVGLNPTRRGAALARDIAHADCQLVLTESAHAELLDGLDPGVRV-INVDSPA--WA--DELAAHRDAEPPFRVADPDDL 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 199 MLLYFTSGTTAKPKLVRHSQRSYPVG--ALSTMFwlGLQPGDVHLnISSPGWAKHA----WSCFFApwnAGATVFVvnQP 272
Cdd:PRK07867 155 FMLIFTSGTSGDPKAVRCTHRKVASAgvMLAQRF--GLGPDDVCY-VSMPLFHSNAvmagWAVALA---AGASIAL--RR 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 273 RFDAKSLLATIGRCGVT--TLCAPPTVWRMFIQEKLADYKVSLREVCG--AGeplnPEVIDQVNAAWGATIRDGYGQTET 348
Cdd:PRK07867 227 KFSASGFLPDVRRYGATyaNYVGKPLSYVLATPERPDDADNPLRIVYGneGA----PGDIARFARRFGCVVVDGFGSTEG 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 349 TAMVGNSPGQPVkiGSMGRPLPGyvVRITDAD-GHPAKEGEITLA-----------LGDARPAGLMQGY----QGEGGKL 412
Cdd:PRK07867 303 GVAITRTPDTPP--GALGPLPPG--VAIVDPDtGTECPPAEDADGrllnadeaigeLVNTAGPGGFEGYyndpEADAERM 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 413 SGAegdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTAD 492
Cdd:PRK07867 379 RGG---VYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPG 455
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 91684220 493 AERSRDtALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:PRK07867 456 AKFDPD-AFAEFLAAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSA 503
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
77-541 |
2.37e-36 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 140.91 E-value: 2.37e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 77 RSNQVANYLRAQGLKRGDhLLLLLGNVVPLWET-MLAAIKLGIVVIPattlltpdelrdrLDRGAAKAvvaapdqvakfa 155
Cdd:cd17653 31 ASNALANRLLQLGVVPGD-VVPLLSDRSLEMLVaILAILKAGAAYVP-------------LDAKLPSA------------ 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 156 dlggdsLIRIVVgspqdgwrgydEASQQSDSFTPDGPtqaDDPMLLYFTSGTTAKPKLVRHSQRSYpVGALSTM-FWLGL 234
Cdd:cd17653 85 ------RIQAIL-----------RTSGATLLLTTDSP---DDLAYIIFTSGSTGIPKGVMVPHRGV-LNYVSQPpARLDV 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 235 QPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVvnqprfDAKSLLATIGRcGVTTLCAPPTVWRMFiqeKLADYKvSLR 314
Cdd:cd17653 144 GPGSRVAQVLSIAFDACIGEIFSTLCNGGTLVLA------DPSDPFAHVAR-TVDALMSTPSILSTL---SPQDFP-NLK 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 315 EVCGAGEPLNPEVIDqvnaAW--GATIRDGYGQTETT---AMVGNSPGQPVKIGsmgRPLPGYVVRITDADGHPAKE--- 386
Cdd:cd17653 213 TIFLGGEAVPPSLLD----RWspGRRLYNAYGPTECTissTMTELLPGQPVTIG---KPIPNSTCYILDADLQPVPEgvv 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 387 GEITLAlGdarpAGLMQGYQGEGGK-----LSGAEGD---IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELES 458
Cdd:cd17653 286 GEICIS-G----VQVARGYLGNPALtaskfVPDPFWPgsrMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEE 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 459 -ILLEHEAVAEAAVVPSPDA-IKLAIPkayvlLTADAERSRDtalSIFRHMQQRLAPfKRIRKLElvtELPKTISGKIRR 536
Cdd:cd17653 361 vVLQSQPEVTQAAAIVVNGRlVAFVTP-----ETVDVDGLRS---ELAKHLPSYAVP-DRIIALD---SFPLTANGKVDR 428
|
....*
gi 91684220 537 VHLRR 541
Cdd:cd17653 429 KALRE 433
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
42-540 |
4.15e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 141.66 E-value: 4.15e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 42 AELATNADSRDRAALWIVDGitGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVI 121
Cdd:PRK06188 15 GHLLVSALKRYPDRPALVLG--DTRL--TYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 122 PATTLLTPDELRDRLDRGAAKAVVAAPdqvAKFADLGG------DSLIRIVVGSPQDGWRGYDEASQQSDSFTPDGPTQA 195
Cdd:PRK06188 91 ALHPLGSLDDHAYVLEDAGISTLIVDP---APFVERALallarvPSLKHVLTLGPVPDGVDLLAAAAKFGPAPLVAAALP 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPKLVRHSQRSY---PVGALSTMFWlglqPGDVHLNISSPgwAKHAWSCFFAPWNA-GATVFVvnQ 271
Cdd:PRK06188 168 PDIAGLAYTGGTTGKPKGVMGTHRSIatmAQIQLAEWEW----PADPRFLMCTP--LSHAGGAFFLPTLLrGGTVIV--L 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 PRFDAKSLLATIGRCGVT-TLCAPPTVWRMFIQEKLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTE-- 347
Cdd:PRK06188 240 AKFDPAEVLRAIEEQRITaTFLVPTMIYALLDHPDLRTRDLSsLETVYYGASPMSPVRLAEAIERFGPIFAQYYGQTEap 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 348 ----TTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEitlaLGD--ARPAGLMQGYQGEGGKLSGA-EGDIY 420
Cdd:PRK06188 320 mvitYLRKRDHDPDDPKRLTSCGRPTPGLRVALLDEDGREVAQGE----VGEicVRGPLVMDGYWNRPEETAEAfRDGWL 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 421 RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAerSRDTA 500
Cdd:PRK06188 396 HTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGA--AVDAA 473
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 91684220 501 lSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK06188 474 -ELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALR 512
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
28-540 |
9.73e-36 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 140.88 E-value: 9.73e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 28 PDPAPFNWALDWF---DAELATnadsrDRAALwiVDGITGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVV 104
Cdd:PLN02330 21 SVPVPDKLTLPDFvlqDAELYA-----DKVAF--VEAVTGKAV--TYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVA 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 105 PLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLGgdsLIRIVVGSPQD----GWRGYDEA 180
Cdd:PLN02330 92 EYGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVTNDTNYGKVKGLG---LPVIVLGEEKIegavNWKELLEA 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 181 SQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGlqpGDVHLNISSPGWAK--HAWS---- 254
Cdd:PLN02330 169 ADRAGDTSDNEEILQTDLCALPFSSGTTGISKGVMLTHRNLVANLCSSLFSVG---PEMIGQVVTLGLIPffHIYGitgi 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 255 CFFAPWNAGATVfVVNqpRFDAKSLL-ATIGRCGVTTLCAPPTVWRMF---IQEKLADYKVSLREVCGAGEPLNPEVIDQ 330
Cdd:PLN02330 246 CCATLRNKGKVV-VMS--RFELRTFLnALITQEVSFAPIVPPIILNLVknpIVEEFDLSKLKLQAIMTAAAPLAPELLTA 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 331 VNAAW-GATIRDGYGQTETTAMV---GN-SPGQPV-KIGSMGRPLPGYVVRITDAD-GHPAKE---GEITLalgdaRPAG 400
Cdd:PLN02330 323 FEAKFpGVQVQEAYGLTEHSCITlthGDpEKGHGIaKKNSVGFILPNLEVKFIDPDtGRSLPKntpGELCV-----RSQC 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 401 LMQGY---QGEGGKLSGAEGDIYrSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA 477
Cdd:PLN02330 398 VMQGYynnKEETDRTIDEDGWLH-TGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDE 476
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 91684220 478 IKLAIPKAYVLLTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PLN02330 477 EAGEIPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLK 536
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
87-572 |
1.99e-35 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 139.78 E-value: 1.99e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 87 AQGLKRGD---HLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLI 163
Cdd:PRK13388 43 LIALADPDrplHVGVLLGNTPEMLFWLAAAALGGYVLVGLNTTRRGAALAADIRRADCQLLVTDAEHRPLLDGLDLPGVR 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 164 RIVVGSPQdgwrgYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDV---- 239
Cdd:PRK13388 123 VLDVDTPA-----YAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRALTERFGLTRDDVcyvs 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 240 ----HLNISSPGWAkhawscffAPWNAGATvfVVNQPRFDAKSLLATIGRCGVT--TLCAPPTVWRMFIQEKLADYKVSL 313
Cdd:PRK13388 198 mplfHSNAVMAGWA--------PAVASGAA--VALPAKFSASGFLDDVRRYGATyfNYVGKPLAYILATPERPDDADNPL 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 314 REvcGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVkiGSMGRPLPGYV-----------VRITDADGH 382
Cdd:PRK13388 268 RV--AFGNEASPRDIAEFSRRFGCQVEDGYGSSEGAVIVVREPGTPP--GSIGRGAPGVAiynpetltecaVARFDAHGA 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 383 PAKEGEITLALGDARPAGLMQGYQgeggKLSGAEGD-----IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:PRK13388 344 LLNADEAIGELVNTAGAGFFEGYY----NNPEATAErmrhgMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIE 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRV 537
Cdd:PRK13388 420 RILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPD-AFAAFLAAQPDLGTKAWPRYVRIAADLPSTATNKVLKR 498
|
490 500 510
....*....|....*....|....*....|....*....
gi 91684220 538 HLRRLEHDDDRADTL----RGVEYREEDFPElKAAPAKE 572
Cdd:PRK13388 499 ELIAQGWATGDPVTLwvrrGGPAYRLMSEPA-KAALAAE 536
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
70-536 |
5.46e-35 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 141.63 E-value: 5.46e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRL-----DRGAAkaV 144
Cdd:PRK12316 538 DYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVP----LDPEYPAERLaymleDSGVQ--L 611
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VAAPDQVAKFADLGGdSLIRIVVGSPQDGWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAKPKLV--RHSQRSYP 222
Cdd:PRK12316 612 LLSQSHLGRKLPLAA-GVQVLDLDRPAAWLEGYSEEN-------PGTELNPENLAYVIYTSGSTGKPKGAgnRHRALSNR 683
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 223 VGALSTMFWLGLqpGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVNQP-RFDAKSLLATIGRCGVTTLCAPPTVWRMF 301
Cdd:PRK12316 684 LCWMQQAYGLGV--GDTVLQKTPFSFDVSVWE-FFWPLMSGARLVVAAPGdHRDPAKLVELINREGVDTLHFVPSMLQAF 760
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 302 IQEKLADYKVSLREVCGAGEPLNPEVIDQVNA-AWGATIRDGYGQTETTAMVGNSP-----GQPVKIGsmgRPLPGYVVR 375
Cdd:PRK12316 761 LQDEDVASCTSLRRIVCSGEALPADAQEQVFAkLPQAGLYNLYGPTEAAIDVTHWTcveegGDSVPIG---RPIANLACY 837
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 376 ITDADGHPAK---EGEITLAlgdarPAGLMQGYQGEGGKLS--------GAEGDIYRSGDVAFADDDGYLTFVGRTDDVF 444
Cdd:PRK12316 838 ILDANLEPVPvgvLGELYLA-----GRGLARGYHGRPGLTAerfvpspfVAGERMYRTGDLARYRADGVIEYAGRIDHQV 912
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 445 KSSDYRISPFELESILLEHEAVAEAAVVpSPDAIKLAipkAYVLLTADAERSRDTALSifrHMQQRLAPFKRIRKLELVT 524
Cdd:PRK12316 913 KLRGLRIELGEIEARLLEHPWVREAAVL-AVDGKQLV---GYVVLESEGGDWREALKA---HLAASLPEYMVPAQWLALE 985
|
490
....*....|..
gi 91684220 525 ELPKTISGKIRR 536
Cdd:PRK12316 986 RLPLTPNGKLDR 997
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
52-551 |
1.40e-34 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 138.88 E-value: 1.40e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAALWIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDE 131
Cdd:PLN02654 104 DKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAES 183
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 132 LRDRLDRGAAKAV-----VAAPDQVAKFADLGGDSLIRivvgSPQDGWR-----GYDE--ASQQSDS------------F 187
Cdd:PLN02654 184 LAQRIVDCKPKVVitcnaVKRGPKTINLKDIVDAALDE----SAKNGVSvgiclTYENqlAMKREDTkwqegrdvwwqdV 259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 188 TPDGPTQ-------ADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM-FWLGLQPGDVHLNISSPGWAKHAWSCFFAP 259
Cdd:PLN02654 260 VPNYPTKcevewvdAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFkYAFDYKPTDVYWCTADCGWITGHSYVTYGP 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 260 WNAGATVFVvnqprFDAKSLLATIGRC-------GVTTLCAPPTVWRMFIQ---EKLADY-KVSLREVCGAGEPLNPevi 328
Cdd:PLN02654 340 MLNGATVLV-----FEGAPNYPDSGRCwdivdkyKVTIFYTAPTLVRSLMRdgdEYVTRHsRKSLRVLGSVGEPINP--- 411
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 329 dqvnAAWG----------ATIRDGYGQTETTA-MVGNSPGQ-PVKIGSMGRPLPGYVVRITDADGHPAkEGEIT--LALG 394
Cdd:PLN02654 412 ----SAWRwffnvvgdsrCPISDTWWQTETGGfMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGKEI-EGECSgyLCVK 486
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 395 DARPAGLMQGY----QGEGGKLSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAA 470
Cdd:PLN02654 487 KSWPGAFRTLYgdheRYETTYFKPFAG-YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAA 565
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 471 VVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRL--EHDDDR 548
Cdd:PLN02654 566 VVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIasRQLDEL 645
|
...
gi 91684220 549 ADT 551
Cdd:PLN02654 646 GDT 648
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
192-542 |
1.67e-34 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 136.31 E-value: 1.67e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 192 PTQADDPMLLYFTSGTTAKPKLVRHSQRS--YPVGALSTMFwlGLQPGDVHLNISSPgwaKHAW---SCFFAPWNAGATV 266
Cdd:cd05909 143 PVQPDDPAVILFTSGSEGLPKGVVLSHKNllANVEQITAIF--DPNPEDVVFGALPF---FHSFgltGCLWLPLLSGIKV 217
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 267 FVVNQPrFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQT 346
Cdd:cd05909 218 VFHPNP-LDYKKIPELIYDKKATILLGTPTFLRGYARAAHPEDFSSLRLVVAGAEKLKDTLRQEFQEKFGIRILEGYGTT 296
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 347 ETTAMVG-NSPGQPVKIGSMGRPLPGYVVRITDADGH-PAKEGEITLALgdARPAGLMQGYQGEGGKLSGAEGD-IYRSG 423
Cdd:cd05909 297 ECSPVISvNTPQSPNKEGTVGRPLPGMEVKIVSVETHeEVPIGEGGLLL--VRGPNVMLGYLNEPELTSFAFGDgWYDTG 374
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 424 DVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAV-AEAAVVPSPDAIKlaiPKAYVLLTADAERSRDTALS 502
Cdd:cd05909 375 DIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEILPEdNEVAVVSVPDGRK---GEKIVLLTTTTDTDPSSLND 451
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 91684220 503 IFRHMQ-QRLAPFKRIRKlelVTELPKTISGKIRRVHLRRL 542
Cdd:cd05909 452 ILKNAGiSNLAKPSYIHQ---VEEIPLLGTGKPDYVTLKAL 489
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
202-542 |
1.72e-34 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 133.76 E-value: 1.72e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 202 YF-TSGTTAKPKLVRHSqrsypVGALSTMFWLG-----LQPGDV--------HLNISSPgwakhawsCFFAPWNAGATVF 267
Cdd:cd05944 7 YFhTGGTTGTPKLAQHT-----HSNEVYNAWMLalnslFDPDDVllcglplfHVNGSVV--------TLLTPLASGAHVV 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 268 VVNQPRFDAKSLLAT----IGRCGVTTLCAPPTVWRMFIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGY 343
Cdd:cd05944 74 LAGPAGYRNPGLFDNfwklVERYRITSLSTVPTVYAALLQVPVNADISSLRFAMSGAAPLPVELRARFEDATGLPVVEGY 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 344 GQTETTAMVG-NSPGQPVKIGSMGRPLPGYVVRI--TDADGHPAKE------GEITLAlgdarPAGLMQGY-QGEGGKLS 413
Cdd:cd05944 154 GLTEATCLVAvNPPDGPKRPGSVGLRLPYARVRIkvLDGVGRLLRDcapdevGEICVA-----GPGVFGGYlYTEGNKNA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 414 GAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADA 493
Cdd:cd05944 229 FVADGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGA 308
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 91684220 494 ERSrDTALSIF--RHMQQRLAPFKRIrklELVTELPKTISGKIRRVHLRRL 542
Cdd:cd05944 309 VVE-EEELLAWarDHVPERAAVPKHI---EVLEELPVTAVGKVFKPALRAD 355
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
82-540 |
2.03e-34 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 135.97 E-value: 2.03e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 82 ANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDElrdrldrGAAKAVVAAPDQVAKFADLGGds 161
Cdd:cd05929 31 ARAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAPRAE-------ACAIIEIKAAALVCGLFTGGG-- 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 162 lirivvgsPQDGWRGYDEASQQSDSfTPDGPTQADDPMLLyfTSGTTAKPKLVR--HSQRSYPVGAL---STMFWLGlqP 236
Cdd:cd05929 102 --------ALDGLEDYEAAEGGSPE-TPIEDEAAGWKMLY--SGGTTGRPKGIKrgLPGGPPDNDTLmaaALGFGPG--A 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 237 GDVHLnisSPGWAKHAwscffAP--WNAGATVF---VVNQPRFDAKSLLATIGRCGVTTLCAPPTVW-RMFiqeKLAD-- 308
Cdd:cd05929 169 DSVYL---SPAPLYHA-----APfrWSMTALFMggtLVLMEKFDPEEFLRLIERYRVTFAQFVPTMFvRLL---KLPEav 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 309 ---YKV-SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVK-IGSMGRPLPGyVVRITDADGH- 382
Cdd:cd05929 238 rnaYDLsSLKRVIHAAAPCPPWVKEQWIDWGGPIIWEYYGGTEGQGLTIINGEEWLThPGSVGRAVLG-KVHILDEDGNe 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 383 --PAKEGEITLALGDARPAGlmqgYQGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESIL 460
Cdd:cd05929 317 vpPGEIGEVYFANGPGFEYT----NDPEKTAAARNEGGWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENAL 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 461 LEHEAVAEAAVVPSPDAIKLAIPKAyVLLTADAERSRDT-ALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHL 539
Cdd:cd05929 393 IAHPKVLDAAVVGVPDEELGQRVHA-VVQPAPGADAGTAlAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLL 471
|
.
gi 91684220 540 R 540
Cdd:cd05929 472 R 472
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
37-536 |
6.24e-33 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 133.20 E-value: 6.24e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 37 LDWFDAELATNADsrdRAALWIVDGITgvelkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGN----VVPLWetmlA 112
Cdd:PRK05605 35 VDLYDNAVARFGD---RPALDFFGATT------TYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNcpqhIVAFY----A 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 113 AIKLGIVVIPATTLLTPDELRDRL-DRGAAKAVVAapDQVAK-FADLGGDSLIRIVVG---------------------- 168
Cdd:PRK05605 102 VLRLGAVVVEHNPLYTAHELEHPFeDHGARVAIVW--DKVAPtVERLRRTTPLETIVSvnmiaampllqrlalrlpipal 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 169 --------SPQDG---WRGYDEASQQSDSFTPDGPT-QADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWL-GLQ 235
Cdd:PRK05605 180 rkaraaltGPAPGtvpWETLVDAAIGGDGSDVSHPRpTPDDVALILYTSGTTGKPKGAQLTHRNLFANAAQGKAWVpGLG 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 236 PGDVHLNISSPGWakHAWS---CF-FAPWNAGATVFVvnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMfIQEKLADYKV 311
Cdd:PRK05605 260 DGPERVLAALPMF--HAYGltlCLtLAVSIGGELVLL---PAPDIDLILDAMKKHPPTWLPGVPPLYEK-IAEAAEERGV 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 312 SLREV----CGAgEPLNPEVIDQVNAAWGATIRDGYGQTETTAM-VGNSPGQPVKIGSMGRPLPGYVVRITDADGH---- 382
Cdd:PRK05605 334 DLSGVrnafSGA-MALPVSTVELWEKLTGGLLVEGYGLTETSPIiVGNPMSDDRRPGYVGVPFPDTEVRIVDPEDPdetm 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 383 -PAKEGEItlalgdarpagLMQGYQGEGGKLSGAE-------GDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPF 454
Cdd:PRK05605 413 pDGEEGEL-----------LVRGPQVFKGYWNRPEetaksflDGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPA 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 455 ELESILLEHEAVAEAAVV--PSPD-------AIKLAiPKAyvllTADAERSRDtalsifrHMQQRLAPFKRIRKLELVTE 525
Cdd:PRK05605 482 EVEEVLREHPGVEDAAVVglPREDgseevvaAVVLE-PGA----ALDPEGLRA-------YCREHLTRYKVPRRFYHVDE 549
|
570
....*....|.
gi 91684220 526 LPKTISGKIRR 536
Cdd:PRK05605 550 LPRDQLGKVRR 560
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
70-536 |
6.85e-33 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 130.84 E-value: 6.85e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLdrgaakavvaapd 149
Cdd:cd17652 14 TYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLP----LDPAYPAERI------------- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfADLGGDSLIRIVVgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPK--LVRHSQRSYPVGALS 227
Cdd:cd17652 77 -----AYMLADARPALLL-------------------------TTPDNLAYVIYTSGSTGRPKgvVVTHRGLANLAAAQI 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 228 TMFwlGLQPGDVHLNISSPGWAKHAWSCFFApWNAGATVFVVNQPRFDA-KSLLATIGRCGVTTLCAPPTVWRMFIQEKL 306
Cdd:cd17652 127 AAF--DVGPGSRVLQFASPSFDASVWELLMA-LLAGATLVLAPAEELLPgEPLADLLREHRITHVTLPPAALAALPPDDL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 307 ADykvsLREVCGAGEPLNPEVIDQvnaaWGATIR--DGYGQTETT----AMVGNSPGQPVKIGsmgRPLPGYVVRITDAD 380
Cdd:cd17652 204 PD----LRTLVVAGEACPAELVDR----WAPGRRmiNAYGPTETTvcatMAGPLPGGGVPPIG---RPVPGTRVYVLDAR 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 381 GHPAK---EGEITLAlgdarPAGLMQGYQGEGG---------KLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSD 448
Cdd:cd17652 273 LRPVPpgvPGELYIA-----GAGLARGYLNRPGltaerfvadPFGAPGSRMYRTGDLARWRADGQLEFLGRADDQVKIRG 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 449 YRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAersRDTALSIFRHMQQRLAPFKRIRKLELVTELPK 528
Cdd:cd17652 348 FRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGA---APTAAELRAHLAERLPGYMVPAAFVVLDALPL 424
|
....*...
gi 91684220 529 TISGKIRR 536
Cdd:cd17652 425 TPNGKLDR 432
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
197-536 |
7.60e-33 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 128.29 E-value: 7.60e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSypvgalstmfWLGLQPGDVHL-NISS------PGWAKHA---WSCFFAPWNAGAtv 266
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERS----------WIESFVCNEDLfNISGedailaPGPLSHSlflYGAISALYLGGT-- 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 267 fVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKVslREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQ 345
Cdd:cd17633 69 -FIGQRKFNPKSWIRKINQYNATVIYLVPTMLQALARTLEPESKI--KSIFSSGQKLFESTKKKLKNIFpKANLIEFYGT 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 346 TETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGhpakeGEI-TLALgdaRPAGLMQGYQGEGGKlsgAEGDIYRSGD 424
Cdd:cd17633 146 SELSFITYNFNQESRPPNSVGRPFPNVEIEIRNADG-----GEIgKIFV---KSEMVFSGYVRGGFS---NPDGWMSVGD 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 425 VAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadAERSRDTALSIF 504
Cdd:cd17633 215 IGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYS----GDKLTYKQLKRF 290
|
330 340 350
....*....|....*....|....*....|..
gi 91684220 505 rhMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:cd17633 291 --LKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
79-540 |
9.47e-33 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 131.44 E-value: 9.47e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 79 NQVANYLRAQGlKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLG 158
Cdd:PRK07638 37 CKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMIVTERYKLNDLPDEE 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 159 GdSLIRIvvgspqDGWRGYDEASQQSDSFTPDgptQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGD 238
Cdd:PRK07638 116 G-RVIEI------DEWKRMIEKYLPTYAPIEN---VQNAPFYMGFTSGSTGKPKAFLRAQQSWLHSFDCNVHDFHMKRED 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 239 vhlNISSPGWAKH------AWSCFFApwnaGATVFVvnQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKladykvs 312
Cdd:PRK07638 186 ---SVLIAGTLVHslflygAISTLYV----GQTVHL--MRKFIPNQVLDKLETENISVMYTVPTMLESLYKEN------- 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 313 lrevcgaGEPLNPEVIDQVNAAWGA-------------TIRDGYGQTETTAMVGNSPGQPV-KIGSMGRPLPGYVVRITD 378
Cdd:PRK07638 250 -------RVIENKMKIISSGAKWEAeakekiknifpyaKLYEFYGASELSFVTALVDEESErRPNSVGRPFHNVQVRICN 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 379 ADGHPAKEGEITLALgdARPAGLMQGYQGEGGKLSGAEGDIYRS-GDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELE 457
Cdd:PRK07638 323 EAGEEVQKGEIGTVY--VKSPQFFMGYIIGGVLARELNADGWMTvRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIE 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 458 SILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRdtalsifRHMQQRLAPFKRIRKLELVTELPKTISGKIRRV 537
Cdd:PRK07638 401 SVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGSATKQQLK-------SFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARM 473
|
...
gi 91684220 538 HLR 540
Cdd:PRK07638 474 EAK 476
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
70-540 |
1.09e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 131.79 E-value: 1.09e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAP- 148
Cdd:PRK06164 37 SRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRARWLVVWPg 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 149 ----DQVAKFADLGGDSLIR----IVVGS---------PQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKP 211
Cdd:PRK06164 117 fkgiDFAAILAAVPPDALPPlraiAVVDDaadatpapaPGARVQLFALPDPAPPAAAGERAADPDAGALLFTTSGTTSGP 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 212 KLVRHSQRSYPVGALSTMFWLGLQPGDVHLnISSPGWAKHAWSCFFAPWNAGATVfvVNQPRFDAKSLLATIGRCGVTTL 291
Cdd:PRK06164 197 KLVLHRQATLLRHARAIARAYGYDPGAVLL-AALPFCGVFGFSTLLGALAGGAPL--VCEPVFDAARTARALRRHRVTHT 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 292 CAPPTVWRMFIQEKLADYKVSLREVCGAGE--PLNPEVIDQVNAAwGATIRDGYGQTETTAMVGNSPGQP---VKIGSMG 366
Cdd:PRK06164 274 FGNDEMLRRILDTAGERADFPSARLFGFASfaPALGELAALARAR-GVPLTGLYGSSEVQALVALQPATDpvsVRIEGGG 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 367 RPL-PGYVVRITDADG----HPAKEGEITLalgdaRPAGLMQGYQGEGGKLSGAEGD--IYRSGDVAFADDDGYLTFVGR 439
Cdd:PRK06164 353 RPAsPEARVRARDPQDgallPDGESGEIEI-----RAPSLMRGYLDNPDATARALTDdgYFRTGDLGYTRGDGQFVYQTR 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 440 TDDVFKSSDYRISPFELESILLEHEAVAEAAVVpspdAIKL---AIPKAYVLLTADAersRDTALSIFRHMQQRLAPFKR 516
Cdd:PRK06164 428 MGDSLRLGGFLVNPAEIEHALEALPGVAAAQVV----GATRdgkTVPVAFVIPTDGA---SPDEAGLMAACREALAGFKV 500
|
490 500
....*....|....*....|....*..
gi 91684220 517 IRKLELVTELPKTISG---KIRRVHLR 540
Cdd:PRK06164 501 PARVQVVEAFPVTESAngaKIQKHRLR 527
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
35-539 |
1.50e-32 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 130.91 E-value: 1.50e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 35 WALDWFDAELATNADSR-DRAAlwIVDGitgvELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAA 113
Cdd:cd05920 12 WQDEPLGDLLARSAARHpDRIA--VVDG----DRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFAL 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 114 IKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAApdqvakfadlggdslirivvgspqDGWRGYDEASQQSDSFTpDGPt 193
Cdd:cd05920 86 LRLGAVPVLALPSHRRSELSAFCAHAEAVAYIVP------------------------DRHAGFDHRALARELAE-SIP- 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 194 qadDPMLLYFTSGTTAKPKLV--RHSQRSYPVGAlsTMFWLGLQPGDVHLNI---------SSPGwakhawscFFAPWNA 262
Cdd:cd05920 140 ---EVALFLLSGGTTGTPKLIprTHNDYAYNVRA--SAEVCGLDQDTVYLAVlpaahnfplACPG--------VLGTLLA 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 263 GATVFVVNQPRFDAksLLATIGRCGVTTLCAPPTVWRMFIQ--EKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIR 340
Cdd:cd05920 207 GGRVVLAPDPSPDA--AFPLIEREGVTVTALVPALVSLWLDaaASRRADLSSLRLLQVGGARLSPALARRVPPVLGCTLQ 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 341 DGYGQTE---TTAMVGNSPgqPVKIGSMGRPL-PGYVVRITDADGHPAKEGEI--TLALGDARPAGLmqgYQGEGGKLSG 414
Cdd:cd05920 285 QVFGMAEgllNYTRLDDPD--EVIIHTQGRPMsPDDEIRVVDEEGNPVPPGEEgeLLTRGPYTIRGY---YRAPEHNARA 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 415 AEGD-IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADA 493
Cdd:cd05920 360 FTPDgFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLRDPP 439
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 91684220 494 ERsrdtALSIFRHMQQR-LAPFKRIRKLELVTELPKTISGKIRRVHL 539
Cdd:cd05920 440 PS----AAQLRRFLRERgLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
52-542 |
1.87e-32 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 129.83 E-value: 1.87e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAAlwIVDGitgvELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLlgnVVPLWETMLAAI----KLGIVVIPattlL 127
Cdd:cd17648 2 DRVA--VVYG----DKRLTYRELNERANRLAHYLLSVAEIRPDDLVGL---VLDKSELMIIAIlavwKAGAAYVP----I 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 128 TPdelrdrldrgaakavvAAPDQVAKFADlgGDSLIRIVVGSPQDgwrgydeasqqsdsftpdgptqaddpmLLY--FTS 205
Cdd:cd17648 69 DP----------------SYPDERIQFIL--EDTGARVVITNSTD---------------------------LAYaiYTS 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 206 GTTAKPK--LVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATI 283
Cdd:cd17648 104 GTTGKPKgvLVEHGSVVNLRTSLSERYFGRDNGDEAVLFFSNYVFDFFVEQMTLALLNGQKLVVPPDEMRFDPDRFYAYI 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 284 GRCGVTTLCAPPTVWRMFIQEKLAdykvSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTET--TAMVGNSPGQPVK 361
Cdd:cd17648 184 NREKVTYLSGTPSVLQQYDLARLP----HLKRVDAAGEEFTAPVFEKLRSRFAGLIINAYGPTETtvTNHKRFFPGDQRF 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 362 IGSMGRPLPGYVVRITDADGHP---AKEGEITLAlGDarpaGLMQGY----------------QGEGGKLSGAEGDIYRS 422
Cdd:cd17648 260 DKSLGRPVRNTKCYVLNDAMKRvpvGAVGELYLG-GD----GVARGYlnrpeltaerflpnpfQTEQERARGRNARLYKT 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 423 GDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLL---TADAERSRDT 499
Cdd:cd17648 335 GDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKEDASQAQSRIQKYLVgyyLPEPGHVPES 414
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 91684220 500 alSIFRHMQQRLAPF---KRIRKLElvtELPKTISGKirrVHLRRL 542
Cdd:cd17648 415 --DLLSFLRAKLPRYmvpARLVRLE---GIPVTINGK---LDVRAL 452
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
40-540 |
8.32e-32 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 128.84 E-value: 8.32e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 40 FDAeLATNADSRDRAALWIVDGITgvelkPSFATMSKRSNQVANYLRAQGLKRGDHLLLllgNVVPLWETM---LAAIKL 116
Cdd:PRK07514 6 FDA-LRAAFADRDAPFIETPDGLR-----YTYGDLDAASARLANLLVALGVKPGDRVAV---QVEKSPEALalyLATLRA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 117 GIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLGGDSLIRIVVGSPQDGwRG--YDEASQQSDSFtPDGPTQ 194
Cdd:PRK07514 77 GAVFLPLNTAYTLAELDYFIGDAEPALVVCDPANFAWLSKIAAAAGAPHVETLDADG-TGslLEAAAAAPDDF-ETVPRG 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 195 ADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALS-TMFWlGLQPGDVHLnisspgwakHAWSCF-----FAPWN----AGA 264
Cdd:PRK07514 155 ADDLAAILYTSGTTGRSKGAMLSHGNLLSNALTlVDYW-RFTPDDVLI---------HALPIFhthglFVATNvallAGA 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 265 TVFVvnQPRFDAKSLLATIGRCgvTTLCAPPTVW-RMFIQEKLAdykvslREVCG------AGE-PLNPEVIDQVNAAWG 336
Cdd:PRK07514 225 SMIF--LPKFDPDAVLALMPRA--TVMMGVPTFYtRLLQEPRLT------REAAAhmrlfiSGSaPLLAETHREFQERTG 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 337 ATIRDGYGQTETtAMVGNSPGQPVKI-GSMGRPLPGYVVRITDAD-GHPAKEGEItlalGDARPAG--LMQGYQGEGGKl 412
Cdd:PRK07514 295 HAILERYGMTET-NMNTSNPYDGERRaGTVGFPLPGVSLRVTDPEtGAELPPGEI----GMIEVKGpnVFKGYWRMPEK- 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 413 SGAE--GD-IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV--VPSPD----AIKLAIP 483
Cdd:PRK07514 369 TAEEfrADgFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVigVPHPDfgegVTAVVVP 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 91684220 484 KAYVLLTADAersrdtalsIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK07514 449 KPGAALDEAA---------ILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
70-536 |
1.09e-31 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 129.38 E-value: 1.09e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGN----VVPLWETMLAaiklGIVVIPATTLLTPDELRDRLDRGAAKAVV 145
Cdd:PRK06710 51 TFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNcpqaVIGYYGTLLA----GGIVVQTNPLYTERELEYQLHDSGAKVIL 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAPDQVAKFADLGGDSLIRIVVGS--------PQDGWRGYDEASQQS--------------DSFTPDGPTQAD---DP-- 198
Cdd:PRK06710 127 CLDLVFPRVTNVQSATKIEHVIVTriadflpfPKNLLYPFVQKKQSNlvvkvsesetihlwNSVEKEVNTGVEvpcDPen 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 199 --MLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWL--GLQPGDVHLNISSpgwakhawscFFAPWNAGATVFV------ 268
Cdd:PRK06710 207 dlALLQYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLynCKEGEEVVLGVLP----------FFHVYGMTAVMNLsimqgy 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 269 --VNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKL-ADYKVSLREVCGAGE-PLNPEVIDQVNAAWGATIRDGYG 344
Cdd:PRK06710 277 kmVLIPKFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSPLlKEYDISSIRACISGSaPLPVEVQEKFETVTGGKLVEGYG 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 345 QTETTAMVGNSPGQPVKI-GSMGRPLPGYVVRITDADG----HPAKEGEITLalgdaRPAGLMQGYQGEGGKLSGAEGDI 419
Cdd:PRK06710 357 LTESSPVTHSNFLWEKRVpGSIGVPWPDTEAMIMSLETgealPPGEIGEIVV-----KGPQIMKGYWNKPEETAAVLQDG 431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 420 Y-RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRD 498
Cdd:PRK06710 432 WlHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEE 511
|
490 500 510
....*....|....*....|....*....|....*...
gi 91684220 499 talSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:PRK06710 512 ---ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILR 546
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
70-536 |
1.70e-31 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 127.83 E-value: 1.70e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDR----LDRGAAKAVV 145
Cdd:cd17655 24 TYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLP----IDPDYPEERiqyiLEDSGADILL 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAPDQVAKFADLGgdSLIRIVVGspqdgwrgyDEASQQSDSFTPDGptQADDPMLLYFTSGTTAKPK--LVRHSQ----- 218
Cdd:cd17655 100 TQSHLQPPIAFIG--LIDLLDED---------TIYHEESENLEPVS--KSDDLAYVIYTSGSTGKPKgvMIEHRGvvnlv 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 219 ----RSYPVGALSTMfwlgLQPGDVHLNISspgwakhAWScFFAPWNAGATVFVVNQ-PRFDAKSLLATIG--RCGVTTL 291
Cdd:cd17655 167 ewanKVIYQGEHLRV----ALFASISFDAS-------VTE-IFASLLSGNTLYIVRKeTVLDGQALTQYIRqnRITIIDL 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 292 caPPTVWRMFIQEKLADYkVSLREVCGAGEPLNPEVIDQVNAAWGA--TIRDGYGQTETT--AMVGNSPGQPVKIGS--M 365
Cdd:cd17655 235 --TPAHLKLLDAADDSEG-LSLKHLIVGGEALSTELAKKIIELFGTnpTITNAYGPTETTvdASIYQYEPETDQQVSvpI 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 GRPLPGYVVRITDADGHPAKEG---EITLAlGDarpaGLMQGYQGE---------------GGKLsgaegdiYRSGDVAF 427
Cdd:cd17655 312 GKPLGNTRIYILDQYGRPQPVGvagELYIG-GE----GVARGYLNRpeltaekfvddpfvpGERM-------YRTGDLAR 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 428 ADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadAERSRDTA-LSIF-- 504
Cdd:cd17655 380 WLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIV----SEKELPVAqLREFla 455
|
490 500 510
....*....|....*....|....*....|..
gi 91684220 505 RHMQQRLAPFKRIRklelVTELPKTISGKIRR 536
Cdd:cd17655 456 RELPDYMIPSYFIK----LDEIPLTPNGKVDR 483
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
70-536 |
2.15e-31 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 128.08 E-value: 2.15e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK05852 45 SYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRVRSQAAGARVVLIDAD 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFA-----------DLGGDSLIRIVVGSPQDGwrgyDEASQQSDSFTPDGpTQADDPMLLyFTSGTTAKPKLV--RH 216
Cdd:PRK05852 125 GPHDRAepttrwwpltvNVGGDSGPSGGTLSVHLD----AATEPTPATSTPEG-LRPDDAMIM-FTGGTTGLPKMVpwTH 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 217 SQRSYPVGALSTMFWLGlqPGDVHLNIsSPGWAKHAW-SCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPP 295
Cdd:PRK05852 199 ANIASSVRAIITGYRLS--PRDATVAV-MPLYHGHGLiAALLATLASGGAVLLPARGRFSAHTFWDDIKAVGATWYTAVP 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 296 TVWRMFIQ----EKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV--------GNSPGQPVKIG 363
Cdd:PRK05852 276 TIHQILLEraatEPSGRKPAALRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEATHQVtttqiegiGQTENPVVSTG 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 364 SMGRPlPGYVVRITDADGH---PAKEGEITLalgdaRPAGLMQGYQGEGGKLSGAEGDIY-RSGDVAFADDDGYLTFVGR 439
Cdd:PRK05852 356 LVGRS-TGAQIRIVGSDGLplpAGAVGEVWL-----RGTTVVRGYLGDPTITAANFTDGWlRTGDLGSLSAAGDLSIRGR 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 440 TDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIFRhmqQRLAPFKRIRK 519
Cdd:PRK05852 430 IKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPTAEELVQFCR---ERLAAFEIPAS 506
|
490
....*....|....*..
gi 91684220 520 LELVTELPKTISGKIRR 536
Cdd:PRK05852 507 FQEASGLPHTAKGSLDR 523
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
70-533 |
6.60e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 126.54 E-value: 6.60e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK07798 30 TYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDELRYLLDDSDAVALVYERE 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADL-----GGDSLIRIVVGSPQDGWRG---YDEASQQSDSFTPDGPTQADDPMLLYfTSGTTAKPKLV--RHSQr 219
Cdd:PRK07798 110 FAPRVAEVlprlpKLRTLVVVEDGSGNDLLPGavdYEDALAAGSPERDFGERSPDDLYLLY-TGGTTGMPKGVmwRQED- 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 220 SYPVGALSTMFWLGLQPGDVH----LNISSPG---------------WAkhAWSCFFapwnAGATVFVVNQPRFDAKSLL 280
Cdd:PRK07798 188 IFRVLLGGRDFATGEPIEDEEelakRAAAGPGmrrfpapplmhgagqWA--AFAALF----SGQTVVLLPDVRFDADEVW 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 281 ATIGRCGVTtlcapptvwRMFIQ------------EKLADYKVS-LREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQT 346
Cdd:PRK07798 262 RTIEREKVN---------VITIVgdamarplldalEARGPYDLSsLFAIASGGALFSPSVKEALLELLpNVVLTDSIGSS 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 347 ET----TAMVgnSPGQPVKIGSMGRPLPGYVVriTDADGHPAKEGEIT---LALGDARPaglmQGYQGEGGK----LSGA 415
Cdd:PRK07798 333 ETgfggSGTV--AKGAVHTGGPRFTIGPRTVV--LDEDGNPVEPGSGEigwIARRGHIP----LGYYKDPEKtaetFPTI 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 416 EGDIYR-SGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDaiklaiPK------AYVL 488
Cdd:PRK07798 405 DGVRYAiPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPD------ERwgqevvAVVQ 478
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 91684220 489 LTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGK 533
Cdd:PRK07798 479 LREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
42-540 |
7.70e-31 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 127.84 E-value: 7.70e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 42 AELATNADSRDRAALWIVDGITGVELKPSFAtmskrsnQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVI 121
Cdd:PRK06060 11 AEQASEAGWYDRPAFYAADVVTHGQIHDGAA-------RLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAF 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 122 pattLLTPDELRDRL---DRGAAKAVVA---------APDQVAKFADLGGDSlIRIvvgSPQDgwrgYDeasqqsdsftp 189
Cdd:PRK06060 84 ----LANPELHRDDHalaARNTEPALVVtsdalrdrfQPSRVAEAAELMSEA-ARV---APGG----YE----------- 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 190 dgPTQADDPMLLYFTSGTTAKPK--LVRHSQRSYPVGALSTMFwLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVf 267
Cdd:PRK06060 141 --PMGGDALAYATYTSGTTGPPKaaIHRHADPLTFVDAMCRKA-LRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSA- 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 268 VVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGAT-IRDGYGQT 346
Cdd:PRK06060 217 VINSAPVTPEAAAILSARFGPSVLYGVPNFFARVIDSCSPDSFRSLRCVVSAGEALELGLAERLMEFFGGIpILDGIGST 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 347 ET-TAMVGNSPGQpVKIGSMGRPLPGYVVRITDADGHPAKEGeitlALGD--ARPAGLMQGYQGEGGKLSgAEGDIYRSG 423
Cdd:PRK06060 297 EVgQTFVSNRVDE-WRLGTLGRVLPPYEIRVVAPDGTTAGPG----VEGDlwVRGPAIAKGYWNRPDSPV-ANEGWLDTR 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 424 DVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSI 503
Cdd:PRK06060 371 DRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDL 450
|
490 500 510
....*....|....*....|....*....|....*..
gi 91684220 504 FRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK06060 451 HRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
76-541 |
1.03e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 126.21 E-value: 1.03e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 76 KRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD--QVAK 153
Cdd:PRK08162 51 ARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVDTEfaEVAR 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 154 --FADLGGDSLIRIVVGSPQD------GWRGYDEASQQSD-SFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVG 224
Cdd:PRK08162 131 eaLALLPGPKPLVIDVDDPEYpggrfiGALDYEAFLASGDpDFAWTLPADEWDAIALNYTSGTTGNPKGVVYHHRGAYLN 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 225 ALSTMFWLGLQPGDV--------HLNisspGWakhawsCFfaPW----NAGATVFVvnqPRFDAKSLLATIGRCGVTTLC 292
Cdd:PRK08162 211 ALSNILAWGMPKHPVylwtlpmfHCN----GW------CF--PWtvaaRAGTNVCL---RKVDPKLIFDLIREHGVTHYC 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 293 APPTVWRMFIQEKLADYKVSLREVCG--AGEPLNPEVIDQVNAAwGATIRDGYGQTET--TAMV--------GNSPGQPV 360
Cdd:PRK08162 276 GAPIVLSALINAPAEWRAGIDHPVHAmvAGAAPPAAVIAKMEEI-GFDLTHVYGLTETygPATVcawqpewdALPLDERA 354
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 361 KIGSM------------------GRPLPgyvvritdADGHPAkeGEITLalgdaRPAGLMQGY-QGEGGKLSGAEGDIYR 421
Cdd:PRK08162 355 QLKARqgvryplqegvtvldpdtMQPVP--------ADGETI--GEIMF-----RGNIVMKGYlKNPKATEEAFAGGWFH 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 422 SGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERsrdTAL 501
Cdd:PRK08162 420 TGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASA---TEE 496
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 91684220 502 SIFRHMQQRLAPFKRIRKLELvTELPKTISGKIRRVHLRR 541
Cdd:PRK08162 497 EIIAHCREHLAGFKVPKAVVF-GELPKTSTGKIQKFVLRE 535
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
197-542 |
1.16e-30 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 122.05 E-value: 1.16e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLnISSP-----GWAKhAWSCFFAPWNagatvFVVNQ 271
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWL-LSLPlyhvgGLAI-LVRSLLAGAE-----LVLLE 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 PRFDaksLLATIGRCGVTTLCAPPTVWRMFIQEKLA-DYKVSLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTA 350
Cdd:cd17630 74 RNQA---LAEDLAPPGVTHVSLVPTQLQRLLDSGQGpAALKSLRAVLLGGAPIPPELLERAADR-GIPLYTTYGMTETAS 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 351 MVGNSPGQPVKIGSMGRPLPGYVVRITDadghpakEGEITLalgdaRPAGLMQGYQGEGGKLSGAEGDIYRSGDVAFADD 430
Cdd:cd17630 150 QVATKRPDGFGRGGVGVLLPGRELRIVE-------DGEIWV-----GGASLAMGYLRGQLVPEFNEDGWFTTKDLGELHA 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 431 DGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAyvLLTADAERSRDTalsIFRHMQQR 510
Cdd:cd17630 218 DGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVA--VIVGRGPADPAE---LRAWLKDK 292
|
330 340 350
....*....|....*....|....*....|..
gi 91684220 511 LAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:cd17630 293 LARFKLPKRIYPVPELPRTGGGKVDRRALRAW 324
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
66-542 |
3.87e-30 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 124.50 E-value: 3.87e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 66 ELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV 145
Cdd:PRK12583 43 ALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVI 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAP-----DQVA-------KFADLGGDSLI-------RIVV---GSPQDGWRGYDEASQQSDSFTPD------GPTQADD 197
Cdd:PRK12583 123 CADafktsDYHAmlqellpGLAEGQPGALAcerlpelRGVVslaPAPPPGFLAWHELQARGETVSREalaerqASLDRDD 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 198 PMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVhLNISSPGWakHAWSCFFAPW---NAGATVfVVNQPRF 274
Cdd:PRK12583 203 PINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDR-LCVPVPLY--HCFGMVLANLgcmTVGACL-VYPNEAF 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 275 DAKSLLATIGRCGVTTLCAPPTvwrMFIQE----KLADYKVS-LREVCGAGEPLNPE----VIDQVNAAwgaTIRDGYGQ 345
Cdd:PRK12583 279 DPLATLQAVEEERCTALYGVPT---MFIAEldhpQRGNFDLSsLRTGIMAGAPCPIEvmrrVMDEMHMA---EVQIAYGM 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 346 TET---TAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGD--ARPAGLMQGYQG--EGGKLSGAEGD 418
Cdd:PRK12583 353 TETspvSLQTTAADDLERRVETVGRTQPHLEVKVVDPDGATVPRGEI----GElcTRGYSVMKGYWNnpEATAESIDEDG 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 419 IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLL----TADAE 494
Cdd:PRK12583 429 WMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLhpghAASEE 508
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 91684220 495 RSRDTAlsifrhmQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:PRK12583 509 ELREFC-------KARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREI 549
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
197-536 |
5.83e-30 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 120.45 E-value: 5.83e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISsPGWAKHAWSCFFAPWNAGATVfvVNQPRFDA 276
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNML-PLFHIAGLNLALATFHAGGAN--VVMEKFDP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 277 KSLLATIGRCGVTTLCA-PPTVWRMFIQEKLADYKV-SLREVCGAGeplNPEVIDQVNAAWGATIRDGYGQTETTAMVGN 354
Cdd:cd17637 78 AEALELIEEEKVTLMGSfPPILSNLLDAAEKSGVDLsSLRHVLGLD---APETIQRFEETTGATFWSLYGQTETSGLVTL 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 355 SPGQPvKIGSMGRPLPGYVVRITDADGHPA---KEGEITLalgdaRPAGLMQGYQGEGgKLSGA--EGDIYRSGDVAFAD 429
Cdd:cd17637 155 SPYRE-RPGSAGRPGPLVRVRIVDDNDRPVpagETGEIVV-----RGPLVFQGYWNLP-ELTAYtfRNGWHHTGDLGRFD 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 430 DDGYLTFVGRT--DDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA-IKLAIpKAYVLLtadAERSRDTALSIFRH 506
Cdd:cd17637 228 EDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPkWGEGI-KAVCVL---KPGATLTADELIEF 303
|
330 340 350
....*....|....*....|....*....|
gi 91684220 507 MQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:cd17637 304 VGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
29-540 |
7.59e-30 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 123.59 E-value: 7.59e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 29 DPAPFNWALDWFDAELATNADsrdraalwivdgitgvelKPSFATMSKR---------SNQVANYLRAQGLKRGDHLLLL 99
Cdd:PRK07059 18 DASQYPSLADLLEESFRQYAD------------------RPAFICMGKAitygeldelSRALAAWLQSRGLAKGARVAIM 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 100 LGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapdqVAKFAD-----LGGDSLIRIVVGSPQD-- 172
Cdd:PRK07059 80 MPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVV----LENFATtvqqvLAKTAVKHVVVASMGDll 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 173 GWRG------------------------YDEASQQ--SDSFTPDgPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGAL 226
Cdd:PRK07059 156 GFKGhivnfvvrrvkkmvpawslpghvrFNDALAEgaRQTFKPV-KLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVL 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 227 STMFWLG---LQPGDV-HLNISSPGWAKHAWS---CFFAPWNAGATVFVVNQPRfDAKSLLATIGRCGVTTLCAPPTVWR 299
Cdd:PRK07059 235 QMEAWLQpafEKKPRPdQLNFVCALPLYHIFAltvCGLLGMRTGGRNILIPNPR-DIPGFIKELKKYQVHIFPAVNTLYN 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 300 MFIQ----EKLaDYKvSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV-GNSPGQPVKIGSMGRPLPGYVV 374
Cdd:PRK07059 314 ALLNnpdfDKL-DFS-KLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSETSPVAtCNPVDATEFSGTIGLPLPSTEV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 375 RITDADGH---PAKEGEITlalgdARPAGLMQGY---QGEGGKLSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSD 448
Cdd:PRK07059 392 SIRDDDGNdlpLGEPGEIC-----IRGPQVMAGYwnrPDETAKVMTADG-FFRTGDVGVMDERGYTKIVDRKKDMILVSG 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 449 YRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadaerSRDTALS---IFRHMQQRLAPFKRIRKLELVTE 525
Cdd:PRK07059 466 FNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFVV-------KKDPALTeedVKAFCKERLTNYKRPKFVEFRTE 538
|
570
....*....|....*
gi 91684220 526 LPKTISGKIRRVHLR 540
Cdd:PRK07059 539 LPKTNVGKILRRELR 553
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
66-536 |
1.06e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 125.45 E-value: 1.06e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 66 ELKPSFATMSKRSNQVANYLRAQG----------LKRGDHLLLLLgnvvplwetmLAAIKLGIVVIPattlLTPDELRDR 135
Cdd:PRK12316 4574 EEKLTYAELNRRANRLAHALIARGvgpevlvgiaMERSAEMMVGL----------LAVLKAGGAYVP----LDPEYPRER 4639
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 136 L-----DRGAAKAVVAAPDQVAKFADLGGDSLirivVGSPQDGWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAK 210
Cdd:PRK12316 4640 LaymmeDSGAALLLTQSHLLQRLPIPDGLASL----ALDRDEDWEGFPAHD-------PAVRLHPDNLAYVIYTSGSTGR 4708
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 211 PKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTT 290
Cdd:PRK12316 4709 PKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEG-LYHPLINGASVVIRDDSLWDPERLYAEIHEHRVTV 4787
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 291 LCAPPTVWRMFIQEKLADYKV-SLREVCGAGEPLNPEVIDQV-NAAWGATIRDGYGQTETTAMV---GNSPGQPVKIGSM 365
Cdd:PRK12316 4788 LVFPPVYLQQLAEHAERDGEPpSLRVYCFGGEAVAQASYDLAwRALKPVYLFNGYGPTETTVTVllwKARDGDACGAAYM 4867
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 --GRPLPGYVVRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGGKLS--------GAEGD-IYRSGDVAFADDDGY 433
Cdd:PRK12316 4868 piGTPLGNRSGYVLDGQLNPLPVGVAgELYLGGE---GVARGYLERPALTAerfvpdpfGAPGGrLYRTGDLARYRADGV 4944
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 434 LTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA-----IKLAIPKAYVLLTADAERS--RDtalSIFRH 506
Cdd:PRK12316 4945 IDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAvgkqlVGYVVPQDPALADADEAQAelRD---ELKAA 5021
|
490 500 510
....*....|....*....|....*....|
gi 91684220 507 MQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:PRK12316 5022 LRERLPEYMVPAHLVFLARMPLTPNGKLDR 5051
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
52-542 |
1.13e-29 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 122.27 E-value: 1.13e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 52 DRAALWIVDGitgvELkpSFATMSKRSNQVANYLRAQGLKRGDhlllllgnVVPL------WE--TMLAAIKLGIVVIPa 123
Cdd:cd05918 14 DAPAVCAWDG----SL--TYAELDRLSSRLAHHLRSLGVGPGV--------FVPLcfekskWAvvAMLAVLKAGGAFVP- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 124 ttlLTPDELRDRLdrgaakavvaapdqvakfADLGGDSLIRIVVGSpqdgwrgydeasqqsdsftpdgptQADDPMLLYF 203
Cdd:cd05918 79 ---LDPSHPLQRL------------------QEILQDTGAKVVLTS------------------------SPSDAAYVIF 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 204 TSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVnqPRFDAKSLLA-T 282
Cdd:cd05918 114 TSGSTGKPKGVVIEHRALSTSALAHGRALGLTSESRVLQFASYTFDVSILE-IFTTLAAGGCLCIP--SEEDRLNDLAgF 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 283 IGRCGVTTLCAPPTVWRMFIQEKLadykVSLREVCGAGEPLNPEVIDQvnaaWGATIR--DGYGQTETT-AMVGNSPGQP 359
Cdd:cd05918 191 INRLRVTWAFLTPSVARLLDPEDV----PSLRTLVLGGEALTQSDVDT----WADRVRliNAYGPAECTiAATVSPVVPS 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 360 VKIGSMGRPLPGyVVRITDADGH--PAKEGEI--------TLA---LGDA---------RPAGLMQGYQGEGGKLsgaeg 417
Cdd:cd05918 263 TDPRNIGRPLGA-TCWVVDPDNHdrLVPIGAVgelliegpILArgyLNDPektaaafieDPAWLKQEGSGRGRRL----- 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 418 diYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVV------PSPDAIKLAipkAYVLLTA 491
Cdd:cd05918 337 --YRTGDLVRYNPDGSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVevvkpkDGSSSPQLV---AFVVLDG 411
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 492 DAERSRDTALSIFRHMQQRLAPFKRIR-KLEL-------------VTELPKTISGKIRRVHLRRL 542
Cdd:cd05918 412 SSSGSGDGDSLFLEPSDEFRALVAELRsKLRQrlpsymvpsvflpLSHLPLTASGKIDRRALREL 476
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
70-550 |
3.16e-29 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 122.29 E-value: 3.16e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK08279 64 SYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRGAVLAHSLNLVDAKHLIVGEE 143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGDSLIRIVV-------GSPQDGWRGYDEASQQSDSFTPD--GPTQADDPMLLYFTSGTTAKPKLVRHSQRS 220
Cdd:PRK08279 144 LVEAFEEARADLARPPRLwvaggdtLDDPEGYEDLAAAAAGAPTTNPAsrSGVTAKDTAFYIYTSGTTGLPKAAVMSHMR 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 221 ypvgALSTMFW----LGLQPGDV--------H---LNIsspgwakhAWSCFFApwnAGATvfVVNQPRFDAKSLLATIGR 285
Cdd:PRK08279 224 ----WLKAMGGfgglLRLTPDDVlycclplyHntgGTV--------AWSSVLA---AGAT--LALRRKFSASRFWDDVRR 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 286 CGVTT------LC-----APPtvwrmfiqeKLADYKVSLREVCGAGepLNPEVIDQVNAAWG-ATIRDGYGQTE-TTAMV 352
Cdd:PRK08279 287 YRATAfqyigeLCryllnQPP---------KPTDRDHRLRLMIGNG--LRPDIWDEFQQRFGiPRILEFYAASEgNVGFI 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 gNSPGQPvkiGSMGR-PL----PGYVVR--------ITDADGH--PAKEGEITLALGDARPAGLMQGYQGEGGKLSG--- 414
Cdd:PRK08279 356 -NVFNFD---GTVGRvPLwlahPYAIVKydvdtgepVRDADGRciKVKPGEVGLLIGRITDRGPFDGYTDPEASEKKilr 431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 415 ---AEGDIY-RSGDVAFADDDGYLTFVGRTDDVF--KSSDyrISPFELESILLEHEAVAEAAV--VPSPD--------AI 478
Cdd:PRK08279 432 dvfKKGDAWfNTGDLMRDDGFGHAQFVDRLGDTFrwKGEN--VATTEVENALSGFPGVEEAVVygVEVPGtdgragmaAI 509
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 91684220 479 KLAIPKAYVLltadaersrdTALSifRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDDDRAD 550
Cdd:PRK08279 510 VLADGAEFDL----------AALA--AHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLRKEGFDPSKVD 569
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
70-541 |
5.01e-29 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 121.02 E-value: 5.01e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapD 149
Cdd:PRK13382 70 TWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIY--D 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QvaKFADLGGDSL------IRIVVGSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLyfTSGTTAKPKLVRHSQRSYPV 223
Cdd:PRK13382 148 E--EFSATVDRALadcpqaTRIVAWTDEDHDLTVEVLIAAHAGQRPEPTGRKGRVILL--TSGTTGTPKGARRSGPGGIG 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 GALSTMF---WLGLQPgdvhLNISSPGWakHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRM 300
Cdd:PRK13382 224 TLKAILDrtpWRAEEP----TVIVAPMF--HAWGFSQLVLAASLACTIVTRRRFDPEATLDLIDRHRATGLAVVPVMFDR 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 301 FIQ---EKLADYK-VSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVK-IGSMGRPLPGYVVR 375
Cdd:PRK13382 298 IMDlpaEVRNRYSgRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMIATATPADLRAaPDTAGRPAEGTEIR 377
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 376 ITDADGHPAKEGEITLALgdARPAGLMQGYQgeggklSGAEGDIYR----SGDVAFADDDGYLTFVGRTDDVFKSSDYRI 451
Cdd:PRK13382 378 ILDQDFREVPTGEVGTIF--VRNDTQFDGYT------SGSTKDFHDgfmaSGDVGYLDENGRLFVVGRDDEMIVSGGENV 449
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 452 SPFELESILLEHEAVAEAAVVPSPD---AIKLAipkAYVLLTADAERSRDTALSifrHMQQRLAPFKRIRKLELVTELPK 528
Cdd:PRK13382 450 YPIEVEKTLATHPDVAEAAVIGVDDeqyGQRLA---AFVVLKPGASATPETLKQ---HVRDNLANYKVPRDIVVLDELPR 523
|
490
....*....|...
gi 91684220 529 TISGKIRRVHLRR 541
Cdd:PRK13382 524 GATGKILRRELQA 536
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
70-543 |
8.81e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 122.58 E-value: 8.81e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRL-----DRGAAKAV 144
Cdd:PRK12467 1601 TYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVP----LDPEYPRERLaymieDSGIELLL 1676
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VAAPDQvAKFAdlGGDSLIRIVVGSPQDGWRGYDEASQQSdsftpdgptQADDPMLLY--FTSGTTAKPK--LVRHsqrs 220
Cdd:PRK12467 1677 TQSHLQ-ARLP--LPDGLRSLVLDQEDDWLEGYSDSNPAV---------NLAPQNLAYviYTSGSTGRPKgaGNRH---- 1740
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 221 ypvGALSTMF-----WLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPP 295
Cdd:PRK12467 1741 ---GALVNRLcatqeAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVP 1817
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 296 TVWRMFIQEKLADYK-VSLREVCGAGEPLNPEVIDQVNAAWGAT-IRDGYGQTETTAMVGNSP--------GQPVKIGSm 365
Cdd:PRK12467 1818 SMLQQLLQMDEQVEHpLSLRRVVCGGEALEVEALRPWLERLPDTgLFNLYGPTETAVDVTHWTcrrkdlegRDSVPIGQ- 1896
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 grPLPGYVVRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGG---------KLSGAEGDIYRSGDVAFADDDGYLT 435
Cdd:PRK12467 1897 --PIANLSTYILDASLNPVPIGVAgELYLGGV---GLARGYLNRPAltaerfvadPFGTVGSRLYRTGDLARYRADGVIE 1971
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 436 FVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVP--SPDAIKLAipkAYVLLTADAERSRDTALSIFR-----HMQ 508
Cdd:PRK12467 1972 YLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAqdGANGKQLV---AYVVPTDPGLVDDDEAQVALRailknHLK 2048
|
490 500 510
....*....|....*....|....*....|....*
gi 91684220 509 QRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLE 543
Cdd:PRK12467 2049 ASLPEYMVPAHLVFLARMPLTPNGKLDRKALPAPD 2083
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
68-540 |
2.33e-28 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 119.10 E-value: 2.33e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 68 KPSFATMSKR---------SNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLD 137
Cdd:PRK05677 40 KPAFSNLGKTltygelyklSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFN 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 138 RGAAKAVVAAPDQ-----------------VAKFADLGGdSLIRIVVGS--------------PQdGWRGYDEASQ-QSD 185
Cdd:PRK05677 120 DSGAKALVCLANMahlaekvlpktgvkhviVTEVADMLP-PLKRLLINAvvkhvkkmvpayhlPQ-AVKFNDALAKgAGQ 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 186 SFTPDGPtQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHAWS----CFFAPWN 261
Cdd:PRK05677 198 PVTEANP-QADDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCRALMGSNLNEGCEILIAPLPLYHIYAftfhCMAMMLI 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 262 AGATVFVVNqPRfDAKSLLATIGR------CGVTTLCAPPTVWRMFIQEKLADYKVSLrevcGAGEPLNPEVIDQVNAAW 335
Cdd:PRK05677 277 GNHNILISN-PR-DLPAMVKELGKwkfsgfVGLNTLFVALCNNEAFRKLDFSALKLTL----SGGMALQLATAERWKEVT 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 336 GATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGDARPAG--LMQGY---QGEGG 410
Cdd:PRK05677 351 GCAICEGYGMTETSPVVSVNPSQAIQVGTIGIPVPSTLCKVIDDDGNELPLGEV----GELCVKGpqVMKGYwqrPEATD 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 411 KLSGAEGDIyRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPD-----AIKL-AIPK 484
Cdd:PRK05677 427 EILDSDGWL-KTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDeksgeAIKVfVVVK 505
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 485 AYVLLTADAersrdtalsIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK05677 506 PGETLTKEQ---------VMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELR 552
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
39-549 |
2.91e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 121.04 E-value: 2.91e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 39 WFDAELATNADsrdraALWIVDGitGVELkpSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGI 118
Cdd:PRK12467 3100 LIEAQVARTPE-----APALVFG--DQQL--SYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGG 3170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 119 VVIPattlLTPDELRDRL-----DRGAaKAVVAAPDQVAKFADLGGDSLIRIvvgsPQDGWRGYDEASqqsdsftPDGPT 193
Cdd:PRK12467 3171 AYVP----LDPEYPRERLaymieDSGV-KLLLTQAHLLEQLPAPAGDTALTL----DRLDLNGYSENN-------PSTRV 3234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 194 QADDPMLLYFTSGTTAKPK--LVRHSQRSYPVGALSTMFwlGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVNQ 271
Cdd:PRK12467 3235 MGENLAYVIYTSGSTGKPKgvGVRHGALANHLCWIAEAY--ELDANDRVLLFMSFSFDGAQER-FLWTLICGGCLVVRDN 3311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 PRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQ-EKLADYKvSLREVCGAGEPLNPEVIDQVNAAWG-ATIRDGYGQTETT 349
Cdd:PRK12467 3312 DLWDPEELWQAIHAHRISIACFPPAYLQQFAEdAGGADCA-SLDIYVFGGEAVPPAAFEQVKRKLKpRGLTNGYGPTEAV 3390
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 350 ------AMVGNSPGQPVKIgSMGRPLPGYVVRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGG---------KLS 413
Cdd:PRK12467 3391 vtvtlwKCGGDAVCEAPYA-PIGRPVAGRSIYVLDGQLNPVPVGVAgELYIGGV---GLARGYHQRPSltaerfvadPFS 3466
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 414 GAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV--VPSPDAIKLAipkAYVLLTA 491
Cdd:PRK12467 3467 GSGGRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVlaRDGAGGKQLV---AYVVPAD 3543
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 91684220 492 DAERSRDTalsIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDDDRA 549
Cdd:PRK12467 3544 PQGDWRET---LRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDAKGSRE 3598
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
70-536 |
3.32e-28 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 120.15 E-value: 3.32e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK10252 485 SYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTAD 564
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGDSLIRivvgspqdgwrgYDEASQQSDSfTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYpvgaLSTM 229
Cdd:PRK10252 565 QLPRFADVPDLTSLC------------YNAPLAPQGA-APLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI----VNRL 627
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWL----GLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVfVVNQPRF--DAKSLLATIGRCGVTTLCAPPTVWRMFIQ 303
Cdd:PRK10252 628 LWMqnhyPLTADDVVLQKTPCSFDVSVWE-FFWPFIAGAKL-VMAEPEAhrDPLAMQQFFAEYGVTTTHFVPSMLAAFVA 705
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 ----EKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMV----------GNSPGQPVKIGsmgRPL 369
Cdd:PRK10252 706 sltpEGARQSCASLRQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVswypafgeelAAVRGSSVPIG---YPV 782
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 370 PGYVVRITDADGHPAKEGeitlALGDARPAG--LMQGYQG----------EGGKLSGAEgdIYRSGDVAFADDDGYLTFV 437
Cdd:PRK10252 783 WNTGLRILDARMRPVPPG----VAGDLYLTGiqLAQGYLGrpdltasrfiADPFAPGER--MYRTGDVARWLDDGAVEYL 856
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 438 GRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVP---------SPDAIKLAipkAYVLLTADAERSRDTALSifrHMQ 508
Cdd:PRK10252 857 GRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHAcvinqaaatGGDARQLV---GYLVSQSGLPLDTSALQA---QLR 930
|
490 500
....*....|....*....|....*...
gi 91684220 509 QRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:PRK10252 931 ERLPPHMVPVVLLQLDQLPLSANGKLDR 958
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
58-542 |
3.62e-28 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 118.55 E-value: 3.62e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 58 IVDGitgvELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLD 137
Cdd:PRK10946 42 VICG----ERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVAPVNALFSHQRSELNAYAS 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 138 RGAAKAVVAApDQVAKFADLGGDSLIRIVVGSPQ-------DGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAK 210
Cdd:PRK10946 118 QIEPALLIAD-RQHALFSDDDFLNTLVAEHSSLRvvlllndDGEHSLDDAINHPAEDFTATPSPADEVAFFQLSGGSTGT 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 211 PKLV--RHSQRSYPVGA--------LSTMFWLGLqPGDVHLNISSPGwakhAWSCFFApwnaGATVFVVNQPrfDAKSLL 280
Cdd:PRK10946 197 PKLIprTHNDYYYSVRRsveicgftPQTRYLCAL-PAAHNYPMSSPG----ALGVFLA----GGTVVLAPDP--SATLCF 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 281 ATIGRCGVT-TLCAPPTV--WRMFIQEklADYKVSLR-----EVCGAgePLNPEVIDQVNAAWGATIRDGYGQTEttAMV 352
Cdd:PRK10946 266 PLIEKHQVNvTALVPPAVslWLQAIAE--GGSRAQLAslkllQVGGA--RLSETLARRIPAELGCQLQQVFGMAE--GLV 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 G----NSPGQPVkIGSMGRPL-PGYVVRITDADGHPAKEGEitlalgdarPAGLM-------QGY---QGEGGKLSGAEG 417
Cdd:PRK10946 340 NytrlDDSDERI-FTTQGRPMsPDDEVWVADADGNPLPQGE---------VGRLMtrgpytfRGYyksPQHNASAFDANG 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 418 dIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYvLLTADAERsr 497
Cdd:PRK10946 410 -FYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAF-LVVKEPLK-- 485
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 91684220 498 dtALSIFRH-MQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:PRK10946 486 --AVQLRRFlREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQW 529
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
70-536 |
1.21e-27 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 116.22 E-value: 1.21e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLL----GNVVPLwetmLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV 145
Cdd:cd12114 14 TYGELAERARRVAGALKAAGVRPGDLVAVTLpkgpEQVVAV----LGILAAGAAYVPVDIDQPAARREAILADAGARLVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AapDQVAKFADLGGDSLIrIVVGSPQDGWRgydeasqqsdsFTPDGPTQADDPMLLYFTSGTTAKPK--LVRHSQRSYPV 223
Cdd:cd12114 90 T--DGPDAQLDVAVFDVL-ILDLDALAAPA-----------PPPPVDVAPDDLAYVIFTSGSTGTPKgvMISHRAALNTI 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 GALSTMFwlGLQPGDVHLNISSPGWAKHAWScFFAPWNAGAT-VFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFI 302
Cdd:cd12114 156 LDINRRF--AVGPDDRVLALSSLSFDLSVYD-IFGALSAGATlVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMLL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 QEKLADYKV--SLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAM-----VGNSPGQPVKIgSMGRPLPGYVV 374
Cdd:cd12114 233 DVLEAAQALlpSLRLVLLSGDWIPLDLPARLRALApDARLISLGGATEASIWsiyhpIDEVPPDWRSI-PYGRPLANQRY 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 375 RITDADGHPAKE---GEITLAlGdarpAGLMQGYQGEGGK-----LSGAEGD-IYRSGDVAFADDDGYLTFVGRTDDVFK 445
Cdd:cd12114 312 RVLDPRGRDCPDwvpGELWIG-G----RGVALGYLGDPELtaarfVTHPDGErLYRTGDLGRYRPDGTLEFLGRRDGQVK 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 446 SSDYRISPFELESILLEHEAVAEAAVV--PSPDAIKLAipkAYVllTADAERSRDTALSIFRHMQQRLAPFKRIRKLELV 523
Cdd:cd12114 387 VRGYRIELGEIEAALQAHPGVARAVVVvlGDPGGKRLA---AFV--VPDNDGTPIAPDALRAFLAQTLPAYMIPSRVIAL 461
|
490
....*....|...
gi 91684220 524 TELPKTISGKIRR 536
Cdd:cd12114 462 EALPLTANGKVDR 474
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
197-532 |
1.42e-27 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 113.55 E-value: 1.42e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNiSSPGWAKHAWSCFFAPWNAGAT-VFVvnqPRFD 275
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLN-SGPLFHIGTLMFTLATFHAGGTnVFV---RRVD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 276 AKSLLATIGRCGVT-TLCAPPTVWRM--FIQEKLADYKvSLREVCGAGEPLNPEVIDqvNAAWGATIRdGYGQTETTAMV 352
Cdd:cd17636 77 AEEVLELIEAERCThAFLLPPTIDQIveLNADGLYDLS-SLRSSPAAPEWNDMATVD--TSPWGRKPG-GYGQTEVMGLA 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 G-NSPGQPvKIGSMGRPLPGYVVRITDADGH--PAKE-GEITlalgdARPAGLMQGYQG--EGGKLSGAEGdIYRSGDVA 426
Cdd:cd17636 153 TfAALGGG-AIGGAGRPSPLVQVRILDEDGRevPDGEvGEIV-----ARGPTVMAGYWNrpEVNARRTRGG-WHHTNDLG 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 427 FADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalSIFRH 506
Cdd:cd17636 226 RREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASVTEA---ELIEH 302
|
330 340
....*....|....*....|....*.
gi 91684220 507 MQQRLAPFKRIRKLELVTELPKTISG 532
Cdd:cd17636 303 CRARIASYKKPKSVEFADALPRTAGG 328
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
70-545 |
1.48e-27 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 115.53 E-value: 1.48e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapd 149
Cdd:cd05940 5 TYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsftpdgptqadDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05940 82 -----------------------------------------------DAALYIYTSGTTGLPKAAIISHRRAWRGGAFFA 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDV--------HLNISSPGWAkhawSCFfapwNAGATVfvVNQPRFDAKSLLATIGRCGVTT----------L 291
Cdd:cd05940 115 GSGGALPSDVlytclplyHSTALIVGWS----ACL----ASGATL--VIRKKFSASNFWDDIRKYQATIfqyigelcryL 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 292 CAPPtvwrmfiqEKLADYKVSLREVCGAGepLNPEVIDQVNAAWG-ATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLP 370
Cdd:cd05940 185 LNQP--------PKPTERKHKVRMIFGNG--LRPDIWEEFKERFGvPRIAEFYAATEGNSGFINFFGKPGAIGRNPSLLR 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 371 GY----VVR--------ITDADGH--PAKEGEITLALGDARPAGLMQGYQG----EGGKLSGA--EGDIY-RSGDVAFAD 429
Cdd:cd05940 255 KVaplaLVKydlesgepIRDAEGRciKVPRGEPGLLISRINPLEPFDGYTDpaatEKKILRDVfkKGDAWfNTGDLMRLD 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 430 DDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV--VPSPD--------AIKLAIPKAYVLltadaersrdT 499
Cdd:cd05940 335 GEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVygVQVPGtdgragmaAIVLQPNEEFDL----------S 404
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 91684220 500 ALSifRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHD 545
Cdd:cd05940 405 ALA--AHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRNEGFD 448
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
70-560 |
1.83e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 118.52 E-value: 1.83e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLdrgaakavvaapd 149
Cdd:PRK12316 3084 SYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVP----LDPEYPEERL------------- 3146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfADLGGDSLIRIVVGSPQ------DGWRGY--DEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSY 221
Cdd:PRK12316 3147 -----AYMLEDSGAQLLLSQSHlrlplaQGVQVLdlDRGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSAL 3221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 222 PVGALSTMFWLGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVNQPRFDAKSLL-ATIGRCGVTTLCAPPTVWRM 300
Cdd:PRK12316 3222 SNHLCWMQQAYGLGVGDRVLQFTTFSFDVFVEE-LFWPLMSGARVVLAGPEDWRDPALLvELINSEGVDVLHAYPSMLQA 3300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 301 FIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAawGATIRDGYGQTETT--AMVGNSPGQPVKIGSMGRPLPGYVVRITD 378
Cdd:PRK12316 3301 FLEEEDAHRCTSLKRIVCGGEALPADLQQQVFA--GLPLYNLYGPTEATitVTHWQCVEEGKDAVPIGRPIANRACYILD 3378
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 379 ADGHPAKEGEI-TLALGDarpAGLMQGYQGEGGKLS--------GAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDY 449
Cdd:PRK12316 3379 GSLEPVPVGALgELYLGG---EGLARGYHNRPGLTAerfvpdpfVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGF 3455
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 450 RISPFELESILLEHEAVAEAAVVpspdAIKLAIPKAYVLLTADAERSRDTALSifrHMQQRLAPFKRIRKLELVTELPKT 529
Cdd:PRK12316 3456 RIELGEIEARLLEHPWVREAVVL----AVDGRQLVAYVVPEDEAGDLREALKA---HLKASLPEYMVPAHLLFLERMPLT 3528
|
490 500 510
....*....|....*....|....*....|.
gi 91684220 530 ISGKIRRVHLRRLEHDDDRADTLRGVEYREE 560
Cdd:PRK12316 3529 PNGKLDRKALPRPDAALLQQDYVAPVNELER 3559
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
70-472 |
2.00e-27 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 116.54 E-value: 2.00e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIpattLLTP----DELRDRLDRGAAKAVV 145
Cdd:PRK09274 43 SFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKAGAVPV----LVDPgmgiKNLKQCLAEAQPDAFI 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAPD-QVAKFADLGG-DSLIRIVVGSPQDGWRG--YDEASQQSD--SFTPdGPTQADDPMLLYFTSGTTAKPKLVRHSQR 219
Cdd:PRK09274 119 GIPKaHLARRLFGWGkPSVRRLVTVGGRLLWGGttLATLLRDGAaaPFPM-ADLAPDDMAAILFTSGSTGTPKGVVYTHG 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 220 SY--PVGALSTMFwlGLQPGDVHLnisspgwakhawSCF-----FAPwNAGATVFVvnqPRFDA--------KSLLATIG 284
Cdd:PRK09274 198 MFeaQIEALREDY--GIEPGEIDL------------PTFplfalFGP-ALGMTSVI---PDMDPtrpatvdpAKLFAAIE 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 285 RCGVTTLCAPPTVWrmfiqEKLADYKV-------SLREVCGAGEPLNPEVIDQVNA--AWGATIRDGYGQTE-------- 347
Cdd:PRK09274 260 RYGVTNLFGSPALL-----ERLGRYGEangiklpSLRRVISAGAPVPIAVIERFRAmlPPDAEILTPYGATEalpissie 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 348 -------TTAMVGNSPGQPVkigsmGRPLPGYVVRITDADGHPAKE------------GEITLalgdarpAGLM--QGY- 405
Cdd:PRK09274 335 sreilfaTRAATDNGAGICV-----GRPVDGVEVRIIAISDAPIPEwddalrlatgeiGEIVV-------AGPMvtRSYy 402
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 91684220 406 ----QGEGGKLSGAEGDIY-RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVV 472
Cdd:PRK09274 403 nrpeATRLAKIPDGQGDVWhRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALV 474
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
70-536 |
2.66e-27 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 115.23 E-value: 2.66e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVvaapd 149
Cdd:cd05914 9 TYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAI----- 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydeasqqsdsFTPDgptqADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd05914 84 -------------------------------------FVSD----EDDVALINYTSGTTGNSKGVMLTYRNIVSNVDGVK 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLNISSPGwakHAWSC---FFAPWNAGATVFVVNQ---PRFDAKSLLATIGRCGVttlCAPPTVWRMFIQ 303
Cdd:cd05914 123 EVVLLGKGDKILSILPLH---HIYPLtftLLLPLLNGAHVVFLDKipsAKIIALAFAQVTPTLGV---PVPLVIEKIFKM 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 E------------------------KLADYKVS------LREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMVG 353
Cdd:cd05914 197 DiipkltlkkfkfklakkinnrkirKLAFKKVHeafggnIKEFVIGGAKINPDVEEFLRTI-GFPYTIGYGMTETAPIIS 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 354 NSPGQPVKIGSMGRPLPGYVVRITDADGhPAKEGEITLalgdaRPAGLMQGY--QGEGGKLSGAEGDIYRSGDVAFADDD 431
Cdd:cd05914 276 YSPPNRIRLGSAGKVIDGVEVRIDSPDP-ATGEGEIIV-----RGPNVMKGYykNPEATAEAFDKDGWFHTGDLGKIDAE 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 432 GYLTFVGRTDDVF-KSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSI----FRH 506
Cdd:cd05914 350 GYLYIRGRKKEMIvLSSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLVALAYIDPDFLDVKALKQRNIIDAIkwevRDK 429
|
490 500 510
....*....|....*....|....*....|.
gi 91684220 507 MQQRLAPFKRIRKLELV-TELPKTISGKIRR 536
Cdd:cd05914 430 VNQKVPNYKKISKVKIVkEEFEKTPKGKIKR 460
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
70-541 |
3.97e-27 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 115.46 E-value: 3.97e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLG----NVVPLWETMLAaiklGIVVIPATTLLTPDELRDR---------- 135
Cdd:cd05906 41 SYQDLLEDARRLAAGLRQLGLRPGDSVILQFDdnedFIPAFWACVLA----GFVPAPLTVPPTYDEPNARlrklrhiwql 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 136 LDRGAakaVVAAPDQVAKFADLGGDSLIRIVVGSPQdgwrgydEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVR 215
Cdd:cd05906 117 LGSPV---VLTDAELVAEFAGLETLSGLPGIRVLSI-------EELLDTAADHDLPQSRPDDLALLMLTSGSTGFPKAVP 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 216 HSQRSYPVGALSTMFWLGLQPGDVHLNisspgWA--KHAWS---CFFAPWNAG------ATVFVVNQPRfdakSLLATIG 284
Cdd:cd05906 187 LTHRNILARSAGKIQHNGLTPQDVFLN-----WVplDHVGGlveLHLRAVYLGcqqvhvPTEEILADPL----RWLDLID 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 285 RCGVTTLCAPPTVWRMFIQ--EKLADYK---VSLREVCGAGEPLNPEVIDQVN---AAWGA---TIRDGYGQTETTAMV- 352
Cdd:cd05906 258 RYRVTITWAPNFAFALLNDllEEIEDGTwdlSSLRYLVNAGEAVVAKTIRRLLrllEPYGLppdAIRPAFGMTETCSGVi 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 -GNSPGQP-----VKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGDARPAGLM--QGYQG--EGGKLSGAEGDIYRS 422
Cdd:cd05906 338 ySRSFPTYdhsqaLEFVSLGRPIPGVSMRIVDDEGQLLPEGEV----GRLQVRGPVvtKGYYNnpEANAEAFTEDGWFRT 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 423 GDVAFAdDDGYLTFVGRTDDVFKSSDYRISPFELESIL-----LEHEAVAEAAV-VPSPDAIKLAIpkaYVLLTADAERS 496
Cdd:cd05906 414 GDLGFL-DNGNLTITGRTKDTIIVNGVNYYSHEIEAAVeevpgVEPSFTAAFAVrDPGAETEELAI---FFVPEYDLQDA 489
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 91684220 497 RDTALSIFRHMQQRLAPFKRIRKLEL-VTELPKTISGKIRRVHLRR 541
Cdd:cd05906 490 LSETLRAIRSVVSREVGVSPAYLIPLpKEEIPKTSLGKIQRSKLKA 535
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
201-536 |
4.54e-27 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 112.21 E-value: 4.54e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 201 LYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLnISSPGWAKHAWSC-FFAPWNAGATVfvVNQPRFDAKSL 279
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYL-IINPFFHTFGYKAgIVACLLTGATV--VPVAVFDVDAI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 280 LATIGRCGVTTLCAPPTVWRMFIQE-KLADYKV-SLR-EVCGAGEpLNPEVIDQVNAAWG-ATIRDGYGQTETTAMVGNS 355
Cdd:cd17638 82 LEAIERERITVLPGPPTLFQSLLDHpGRKKFDLsSLRaAVTGAAT-VPVELVRRMRSELGfETVLTAYGLTEAGVATMCR 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 356 PGQPVKI--GSMGRPLPGYVVRITDAdghpakeGEITLalgdaRPAGLMQGY---QGEGGKLSGAEGDIYrSGDVAFADD 430
Cdd:cd17638 161 PGDDAETvaTTCGRACPGFEVRIADD-------GEVLV-----RGYNVMQGYlddPEATAEAIDADGWLH-TGDVGELDE 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 431 DGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLtadAERSRDTALSIFRHMQQR 510
Cdd:cd17638 228 RGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVA---RPGVTLTEEDVIAWCRER 304
|
330 340
....*....|....*....|....*.
gi 91684220 511 LAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:cd17638 305 LANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
70-540 |
5.22e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 114.52 E-value: 5.22e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRgAAKAVVAAPD 149
Cdd:PRK09088 24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQD-AEPRLLLGDD 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKfADLGGDSLIRIVvgspqdgwrgyDEASQQSDSFTPDGPtqADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALstm 229
Cdd:PRK09088 103 AVAA-GRTDVEDLAAFI-----------ASADALEPADTPSIP--PERVSLILFTSGTSGQPKGVMLSERNLQQTAH--- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 fwlglqpgdvhlNISSPGWAKHAwSCFF--APW--------------NAGATVFVVNQprFDAKSLLATIG--RCGVTTL 291
Cdd:PRK09088 166 ------------NFGVLGRVDAH-SSFLcdAPMfhiiglitsvrpvlAVGGSILVSNG--FEPKRTLGRLGdpALGITHY 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 292 CAPPTVWRMFIQEKLADYKV--SLREVCGAGEPLNPEVIdqvnAAW---GATIRDGYGQTETTAMVGNSPGQPV---KIG 363
Cdd:PRK09088 231 FCVPQMAQAFRAQPGFDAAAlrHLTALFTGGAPHAAEDI----LGWlddGIPMVDGFGMSEAGTVFGMSVDCDViraKAG 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 364 SMGRPLPGYVVRITDADGH---PAKEGEITLalgdaRPAGLMQGY---QGEGGKLSGAEGdIYRSGDVAFADDDGYLTFV 437
Cdd:PRK09088 307 AAGIPTPTVQTRVVDDQGNdcpAGVPGELLL-----RGPNLSPGYwrrPQATARAFTGDG-WFRTGDIARRDADGFFWVV 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 438 GRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIpkAYVLLTADAERSRDTALsIFRHMQQRLAPFKRI 517
Cdd:PRK09088 381 DRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEV--GYLAIVPADGAPLDLER-IRSHLSTRLAKYKVP 457
|
490 500
....*....|....*....|...
gi 91684220 518 RKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK09088 458 KHLRLVDALPRTASGKLQKARLR 480
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
70-541 |
6.63e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 116.60 E-value: 6.63e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRL-----DRGAAKAV 144
Cdd:PRK12316 2030 SYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVP----LDPNYPAERLaymleDSGAALLL 2105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VaapdQVAKFADLGGDSLIRIVVGSPQDGWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVG 224
Cdd:PRK12316 2106 T----QRHLLERLPLPAGVARLPLDRDAEWADYPDTA-------PAVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAH 2174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 225 ALSTMFWLGLQPGDVHLNISSPGWAKHAWSCFfAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQE 304
Cdd:PRK12316 2175 CQAAGERYELSPADCELQFMSFSFDGAHEQWF-HPLLNGARVLIRDDELWDPEQLYDEMERHGVTILDFPPVYLQQLAEH 2253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 305 KLAD-YKVSLREVCGAGEPLNPEVIDQVNAAWGAT-IRDGYGQTETTAMVGNSPGQPVKIGS-----MGRPLPGYVVRIT 377
Cdd:PRK12316 2254 AERDgRPPAVRVYCFGGEAVPAASLRLAWEALRPVyLFNGYGPTEAVVTPLLWKCRPQDPCGaayvpIGRALGNRRAYIL 2333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 378 DADGHPAKEGEI-TLALGDArpaGLMQGYQGEGG---------KLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSS 447
Cdd:PRK12316 2334 DADLNLLAPGMAgELYLGGE---GLARGYLNRPGltaerfvpdPFSASGERLYRTGDLARYRADGVVEYLGRIDHQVKIR 2410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 448 DYRISPFELESILLEHEAVAEAAVVP--SPDAIKLAipkAYVLLTADAErsrDTALSIFRHMQQRLAPFKRIRKLELVTE 525
Cdd:PRK12316 2411 GFRIELGEIEARLQAHPAVREAVVVAqdGASGKQLV---AYVVPDDAAE---DLLAELRAWLAARLPAYMVPAHWVVLER 2484
|
490
....*....|....*.
gi 91684220 526 LPKTISGKIRRVHLRR 541
Cdd:PRK12316 2485 LPLNPNGKLDRKALPK 2500
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
71-536 |
2.74e-26 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 114.88 E-value: 2.74e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 71 FATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLdrgaakavvaapdq 150
Cdd:PRK05691 1159 YAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVP----LDPDYPAERL-------------- 1220
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 151 vakfADLGGDSLIRIVVGS-------PQ-DGWRGYDEASQQSDSFTPDGPT---QADDPMLLYFTSGTTAKPKLVRHSQr 219
Cdd:PRK05691 1221 ----AYMLADSGVELLLTQshllerlPQaEGVSAIALDSLHLDSWPSQAPGlhlHGDNLAYVIYTSGSTGQPKGVGNTH- 1295
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 220 sypvGALST-MFWL----GLQPGDVHLNISSPGWAKHAWSCFFaPWNAGATVFV--VNQPRfDAKSLLATIGRCGVTTLC 292
Cdd:PRK05691 1296 ----AALAErLQWMqatyALDDSDVLMQKAPISFDVSVWECFW-PLITGCRLVLagPGEHR-DPQRIAELVQQYGVTTLH 1369
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 293 APPTVWRMFIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAMVGN-----SPGQPVKIGsmg 366
Cdd:PRK05691 1370 FVPPLLQLFIDEPLAAACTSLRRLFSGGEALPAELRNRVLQRLpQVQLHNRYGPTETAINVTHwqcqaEDGERSPIG--- 1446
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 367 RPLPGYVVRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGGKLS--------GAEGD-IYRSGDVAFADDDGYLTF 436
Cdd:PRK05691 1447 RPLGNVLCRVLDAELNLLPPGVAgELCIGGA---GLARGYLGRPALTAerfvpdplGEDGArLYRTGDRARWNADGALEY 1523
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 437 VGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAiklAIPKAYVLLTADAERSrDTALSIFRHMQQRLAPFKR 516
Cdd:PRK05691 1524 LGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREGA---AGAQLVGYYTGEAGQE-AEAERLKAALAAELPEYMV 1599
|
490 500
....*....|....*....|
gi 91684220 517 IRKLELVTELPKTISGKIRR 536
Cdd:PRK05691 1600 PAQLIRLDQMPLGPSGKLDR 1619
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
197-536 |
3.41e-26 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 109.66 E-value: 3.41e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSYpVGALSTMFWLGLQP--GDVHLnisSPGWAKHAwscfFAPWNAGATVF-----VV 269
Cdd:cd17635 2 DPLAVIFTSGTTGEPKAVLLANKTF-FAVPDILQKEGLNWvvGDVTY---LPLPATHI----GGLWWILTCLIhgglcVT 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 270 NQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQE--KLADYKVSLREV-CGAGEPLNPeviDQVNAAWGATIR--DGYG 344
Cdd:cd17635 74 GGENTTYKSLFKILTTNAVTTTCLVPTLLSKLVSElkSANATVPSLRLIgYGGSRAIAA---DVRFIEATGLTNtaQVYG 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 345 QTETTAMVGNSPGQPVK-IGSMGRPLPGYVVRITDADGHP---AKEGEITLalgdaRPAGLMQGYQGEGGKLSGAEGDIY 420
Cdd:cd17635 151 LSETGTALCLPTDDDSIeINAVGRPYPGVDVYLAATDGIAgpsASFGTIWI-----KSPANMLGYWNNPERTAEVLIDGW 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 421 -RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDT 499
Cdd:cd17635 226 vNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASAELDENAIR 305
|
330 340 350
....*....|....*....|....*....|....*..
gi 91684220 500 ALSIfrHMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:cd17635 306 ALKH--TIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
70-541 |
4.31e-26 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 112.15 E-value: 4.31e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLgnvvPLWETM----LAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV 145
Cdd:PRK06087 51 TYSALDHAASRLANWLLAKGIEPGDRVAFQL----PGWCEFtiiyLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFF 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 aAPDQVAK--FADL------GGDSLIRIVV---GSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPK-- 212
Cdd:PRK06087 127 -APTLFKQtrPVDLilplqnQLPQLQQIVGvdkLAPATSSLSLSQIIADYEPLTTAITTHGDELAAVLFTSGTEGLPKgv 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 213 LVRH-----SQRSYPVGalstmfwLGLQPGDVHLnISSPgwAKHAwSCFF----APWNAGATvfVVNQPRFDAKSLLATI 283
Cdd:PRK06087 206 MLTHnnilaSERAYCAR-------LNLTWQDVFM-MPAP--LGHA-TGFLhgvtAPFLIGAR--SVLLDIFTPDACLALL 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 284 GRCGVT-TLCAPPTVWRMF--IQEKLADYKvSLR-EVCGaGEPLNPEVIDQvnaAWGATIR--DGYGQTETTAMVGNSPG 357
Cdd:PRK06087 273 EQQRCTcMLGATPFIYDLLnlLEKQPADLS-ALRfFLCG-GTTIPKKVARE---CQQRGIKllSVYGSTESSPHAVVNLD 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 358 QPVK--IGSMGRPLPGYVVRITDADGHPAKEGEitlaLGD--ARPAGLMQGYQGE---GGKLSGAEGDIYrSGDVAFADD 430
Cdd:PRK06087 348 DPLSrfMHTDGYAAAGVEIKVVDEARKTLPPGC----EGEeaSRGPNVFMGYLDEpelTARALDEEGWYY-SGDLCRMDE 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 431 DGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAER-SRDTALSIFRhmQQ 509
Cdd:PRK06087 423 AGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSlTLEEVVAFFS--RK 500
|
490 500 510
....*....|....*....|....*....|..
gi 91684220 510 RLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:PRK06087 501 RVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
105-541 |
1.19e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 110.08 E-value: 1.19e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 105 PLWETMLA---AIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQvakfadlGGDSLIRIVVGSPQDGWRGYDEAS 181
Cdd:PRK07787 54 PTLATVLAvvgALIAGVPVVPVPPDSGVAERRHILADSGAQAWLGPAPD-------DPAGLPHVPVRLHARSWHRYPEPD 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 182 qqsdsftpdgptqADDPMLLYFTSGTTAKPKLVRHSQRSYPVG--ALSTMfWlGLQPGDVHLnisspgwakHAWSCF--- 256
Cdd:PRK07787 127 -------------PDAPALIVYTSGTTGPPKGVVLSRRAIAADldALAEA-W-QWTADDVLV---------HGLPLFhvh 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 257 ------FAPWNAGATVFVVNQPRFDAkslLATIGRCGVTTLCAPPTVW-RMFIQEKLADYKVSLREVCGAGEPLNPEVID 329
Cdd:PRK07787 183 glvlgvLGPLRIGNRFVHTGRPTPEA---YAQALSEGGTLYFGVPTVWsRIAADPEAARALRGARLLVSGSAALPVPVFD 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 330 QVNAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEITLALGDARPAGLMQGYQG-- 407
Cdd:PRK07787 260 RLAALTGHRPVERYGMTETLITLSTRADGERRPGWVGLPLAGVETRLVDEDGGPVPHDGETVGELQVRGPTLFDGYLNrp 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 408 EGGKLSGAEGDIYRSGDVAFADDDGYLTFVGR-TDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAY 486
Cdd:PRK07787 340 DATAAAFTADGWFRTGDVAVVDPDGMHRIVGReSTDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAY 419
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 487 VLLTADAersrdTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRR 541
Cdd:PRK07787 420 VVGADDV-----AADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLLS 469
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
64-525 |
2.47e-25 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 109.48 E-value: 2.47e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 64 GVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKA 143
Cdd:cd05932 2 GQVVEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 144 V-VAAPDQVAKFADLGGDSLIRIVVgSPQDGWRGYDEASQQSDSFTPDG---PTQADDPMLLYFTSGTTAKPKLVRHSQR 219
Cdd:cd05932 82 LfVGKLDDWKAMAPGVPEGLISISL-PPPSAANCQYQWDDLIAQHPPLEerpTRFPEQLATLIYTSGTTGQPKGVMLTFG 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 220 SYPVGALSTMFWLGLQPGDVHLN------ISSPGWAKHAWscffapWNAGATVFVVNQprfdAKSLLATIGRCGVTTLCA 293
Cdd:cd05932 161 SFAWAAQAGIEHIGTEENDRMLSylplahVTERVFVEGGS------LYGGVLVAFAES----LDTFVEDVQRARPTLFFS 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 294 PPTVWRMF---IQEKLADYKVSL------------REV-------------CGAGePLNPEVIDqvnaaW----GATIRD 341
Cdd:cd05932 231 VPRLWTKFqqgVQDKIPQQKLNLllkipvvnslvkRKVlkglgldqcrlagCGSA-PVPPALLE-----WyrslGLNILE 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 342 GYGQTETTAM-VGNSPGQPvKIGSMGRPLPGYVVRITDadghpakEGEITLalgdaRPAGLMQGYQGEGGKLSGA--EGD 418
Cdd:cd05932 305 AYGMTENFAYsHLNYPGRD-KIGTVGNAGPGVEVRISE-------DGEILV-----RSPALMMGYYKDPEATAEAftADG 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 419 IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYR-ISPFELESILLEHEAVAEAAVVPS--PDAIKLAIPKAYVLLTADAER 495
Cdd:cd05932 372 FLRTGDKGELDADGNLTITGRVKDIFKTSKGKyVAPAPIENKLAEHDRVEMVCVIGSglPAPLALVVLSEEARLRADAFA 451
|
490 500 510
....*....|....*....|....*....|...
gi 91684220 496 SRDTALSIFRHMQQ---RLAPFKRIRKLELVTE 525
Cdd:cd05932 452 RAELEASLRAHLARvnsTLDSHEQLAGIVVVKD 484
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
70-547 |
5.51e-25 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 109.10 E-value: 5.51e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAP 148
Cdd:PRK05620 40 TFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVADP 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 149 ---DQVAKFAD---------LGGDSLIRIVVGSPQDGWRGYD-EASQQSDSFTPDGPTQ-ADDPMLLYFTSGTTAKPKLV 214
Cdd:PRK05620 120 rlaEQLGEILKecpcvravvFIGPSDADSAAAHMPEGIKVYSyEALLDGRSTVYDWPELdETTAAAICYSTGTTGAPKGV 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 215 RHSQRSYPVGALS--TMFWLGLQPGDVHLnISSPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKSLLATIGRCGVTTLC 292
Cdd:PRK05620 200 VYSHRSLYLQSLSlrTTDSLAVTHGESFL-CCVPIYHVLSWGVPLAAFMSGTPL-VFPGPDLSAPTLAKIIATAMPRVAH 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 293 APPTVW-RMFIQ-EKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAmVGNSPGQPVKIG------- 363
Cdd:PRK05620 278 GVPTLWiQLMVHyLKNPPERMSLQEIYVGGSAVPPILIKAWEERYGVDVVHVWGMTETSP-VGTVARPPSGVSgearway 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 364 --SMGRPLPGYVVRITDAD----GHPAKEGEITL-------------ALGDARPAGLMQGYQGEGGKLSGAEGDIYRSGD 424
Cdd:PRK05620 357 rvSQGRFPASLEYRIVNDGqvmeSTDRNEGEIQVrgnwvtasyyhspTEEGGGAASTFRGEDVEDANDRFTADGWLRTGD 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 425 VAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTALSIF 504
Cdd:PRK05620 437 VGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLR 516
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 91684220 505 RHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDDD 547
Cdd:PRK05620 517 DQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQHLADGD 559
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
201-540 |
1.58e-23 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 104.45 E-value: 1.58e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 201 LYFTSGTTAKPKLVRHSQRSYPVGALSTMF--WLGLQPGDVHLNISsPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKS 278
Cdd:PRK06018 182 MCYTSGTTGDPKGVLYSHRSNVLHALMANNgdALGTSAADTMLPVV-PLFHANSWGIAFSAPSMGTKL-VMPGAKLDGAS 259
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 279 LLATIGRCGVTTLCAPPTVWRMFIQE------KLADYKVSlreVCGAGEplNPEVIDQVNAAWGATIRDGYGQTETTAM- 351
Cdd:PRK06018 260 VYELLDTEKVTFTAGVPTVWLMLLQYmekeglKLPHLKMV---VCGGSA--MPRSMIKAFEDMGVEVRHAWGMTEMSPLg 334
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 352 -VGNSPGQPVKIG---------SMGRPLPGYVVRITDADGHP-AKEGEITLALGDARPAgLMQGYQGEGGKLSGAEGdIY 420
Cdd:PRK06018 335 tLAALKPPFSKLPgdarldvlqKQGYPPFGVEMKITDDAGKElPWDGKTFGRLKVRGPA-VAAAYYRVDGEILDDDG-FF 412
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 421 RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVV----PSPDAIKLAIpkayVLLTADAERS 496
Cdd:PRK06018 413 DTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIgvyhPKWDERPLLI----VQLKPGETAT 488
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 91684220 497 RDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK06018 489 RE---EILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALR 529
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
201-545 |
1.37e-22 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 101.71 E-value: 1.37e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 201 LYFTSGTTAKPKLVRHSQRSYPVGALSTMF--WLGLQPGDVHLNISsPGWAKHAWSCFFAPWNAGATVfVVNQPRFDAKS 278
Cdd:PRK07008 181 LCYTSGTTGNPKGALYSHRSTVLHAYGAALpdAMGLSARDAVLPVV-PMFHVNAWGLPYSAPLTGAKL-VLPGPDLDGKS 258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 279 LLATIGRCGVTTLCAPPTVWRMFIQE-KLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTE-----TTAM 351
Cdd:PRK07008 259 LYELIEAERVTFSAGVPTVWLGLLNHmREAGLRFStLRRTVIGGSACPPAMIRTFEDEYGVEVIHAWGMTEmsplgTLCK 338
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 352 VGNSPGQPVKIGSM------GRPLPGYVVRITDADGHPAK-EGEitlALGDARPAG---LMQGYQGEGGKLSGaegDIYR 421
Cdd:PRK07008 339 LKWKHSQLPLDEQRkllekqGRVIYGVDMKIVGDDGRELPwDGK---AFGDLQVRGpwvIDRYFRGDASPLVD---GWFP 412
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 422 SGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTAL 501
Cdd:PRK07008 413 TGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVTREELL 492
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 91684220 502 SIFrhmQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHD 545
Cdd:PRK07008 493 AFY---EGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLREQFRD 533
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
196-544 |
2.09e-22 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 101.03 E-value: 2.09e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISsPGWAKHAWSCFFAPWNAGAT-VFVvnqPRF 274
Cdd:PLN02860 172 DDAVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTA-PLCHIGGLSSALAMLMVGAChVLL---PKF 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 275 DAKSLLATIGRCGVTTLCAPPTVWRMFIQ----EKLADYKVSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTET- 348
Cdd:PLN02860 248 DAKAALQAIKQHNVTSMITVPAMMADLISltrkSMTWKVFPSVRKILNGGGSLSSRLLPDAKKLFpNAKLFSAYGMTEAc 327
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 349 ---TAMVGNSPGQ---------PVKIGSMGRPLPGYVVRitdadGHPAKEGEITLALGDARPAG--------LMQGYQGE 408
Cdd:PLN02860 328 sslTFMTLHDPTLespkqtlqtVNQTKSSSVHQPQGVCV-----GKPAPHVELKIGLDESSRVGriltrgphVMLGYWGQ 402
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 409 GGKLSG---AEGDIyRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKA 485
Cdd:PLN02860 403 NSETASvlsNDGWL-DTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVA 481
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 486 YVLL----------TADAERSRDTALSIFRH--MQQRLAPFKrIRKLELVTE--LPKTISGKIRRVHLRR--LEH 544
Cdd:PLN02860 482 CVRLrdgwiwsdneKENAKKNLTLSSETLRHhcREKNLSRFK-IPKLFVQWRkpFPLTTTGKIRRDEVRRevLSH 555
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
70-539 |
2.18e-22 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 100.84 E-value: 2.18e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLdRGAAKAVVAAPD 149
Cdd:PRK13383 62 SYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALAAAL-RAHHISTVVADN 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFADLGGDSLIRI---VVGSPQDGWRgydeasqqsdsftpdgPTQADDPMLLYFTSGTTAKPKLV-RHSQRSYPVGA 225
Cdd:PRK13383 141 EFAERIAGADDAVAVIdpaTAGAEESGGR----------------PAVAAPGRIVLLTSGTTGKPKGVpRAPQLRSAVGV 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 226 LSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVfvVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFI--- 302
Cdd:PRK13383 205 WVTILDRTRLRTGSRISVAMPMFHGLGLGMLMLTIALGGTV--LTHRHFDAEAALAQASLHRADAFTAVPVVLARILelp 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 -QEKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTE-------TTAMVGNSPGqpvkigSMGRPLPGYVV 374
Cdd:PRK13383 283 pRVRARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEvgigalaTPADLRDAPE------TVGKPVAGCPV 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 375 RITDADGHPAKEGEITLALGDARPAGlmQGYQGEGGKlsGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPF 454
Cdd:PRK13383 357 RILDRNNRPVGPRVTGRIFVGGELAG--TRYTDGGGK--AVVDGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPR 432
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 455 ELESILLEHEAVAEAAVVPSPD---AIKLAipkAYVLL----TADAERSRDtalsifrHMQQRLAPFKRIRKLELVTELP 527
Cdd:PRK13383 433 AVENALAAHPAVADNAVIGVPDerfGHRLA---AFVVLhpgsGVDAAQLRD-------YLKDRVSRFEQPRDINIVSSIP 502
|
490
....*....|..
gi 91684220 528 KTISGKIRRVHL 539
Cdd:PRK13383 503 RNPTGKVLRKEL 514
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
38-540 |
4.36e-22 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 99.89 E-value: 4.36e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 38 DWFDAELATNADsrdRAALWIVDGitgvELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVvPLW-ETMLAAIKL 116
Cdd:PRK08315 20 QLLDRTAARYPD---REALVYRDQ----GLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNV-PEWvLTQFATAKI 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 117 GIVVIPATTLLTPDELRDRLDRGAAKAVVAAP-----DQVAKFADLGGD---------------SLIRIVV--GSPQDGW 174
Cdd:PRK08315 92 GAILVTINPAYRLSELEYALNQSGCKALIAADgfkdsDYVAMLYELAPElatcepgqlqsarlpELRRVIFlgDEKHPGM 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 175 RGYDEASQQSDSFTPD------GPTQADDPMLLYFTSGTTAKPKLVRHSQRS-----YPVGALstmfwLGLQPGDvHLNI 243
Cdd:PRK08315 172 LNFDELLALGRAVDDAelaarqATLDPDDPINIQYTSGTTGFPKGATLTHRNilnngYFIGEA-----MKLTEED-RLCI 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 244 SSPGWakHawsCF------FAPWNAGAT-VFVVnqPRFDAKSLLATI--GRCgvTTLCAPPTvwrMFIQE----KLADYK 310
Cdd:PRK08315 246 PVPLY--H---CFgmvlgnLACVTHGATmVYPG--EGFDPLATLAAVeeERC--TALYGVPT---MFIAEldhpDFARFD 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 311 VS-LREVCGAGEPLNPEVIDQVNAAWGA---TIrdGYGQTETtamvgnSPG--Q-----PV--KIGSMGRPLPGYVVRIT 377
Cdd:PRK08315 314 LSsLRTGIMAGSPCPIEVMKRVIDKMHMsevTI--AYGMTET------SPVstQtrtddPLekRVTTVGRALPHLEVKIV 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 378 DADGH----PAKEGEITlalgdARPAGLMQGYQGEGGKLSGA--EGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRI 451
Cdd:PRK08315 386 DPETGetvpRGEQGELC-----TRGYSVMKGYWNDPEKTAEAidADGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGENI 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 452 SPFELESILLEHEAVAEAAVVPSPDA---------IklaIPKAYVLLTADAERSrdtalsifrHMQQRLAPFKRIRKLEL 522
Cdd:PRK08315 461 YPREIEEFLYTHPKIQDVQVVGVPDEkygeevcawI---ILRPGATLTEEDVRD---------FCRGKIAHYKIPRYIRF 528
|
570
....*....|....*...
gi 91684220 523 VTELPKTISGKIRRVHLR 540
Cdd:PRK08315 529 VDEFPMTVTGKIQKFKMR 546
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
70-546 |
7.73e-22 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 98.65 E-value: 7.73e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapd 149
Cdd:cd05939 5 TFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIF--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfadlggdslirivvgspqdgwrgydEASQQSDSFTPDGPTQADD----PMLLY-FTSGTTAKPK--LVRHSqRSYP 222
Cdd:cd05939 82 -----------------------------NLLDPLLTQSSTEPPSQDDvnfrDKLFYiYTSGTTGLPKaaVIVHS-RYYR 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 223 VGALStMFWLGLQPGDVhLNISSPGWakHAWSCFFAPWNA---GATVfvVNQPRFDAKSLLATIGRCGVTTLCAPPTVWR 299
Cdd:cd05939 132 IAAGA-YYAFGMRPEDV-VYDCLPLY--HSAGGIMGVGQAllhGSTV--VIRKKFSASNFWDDCVKYNCTIVQYIGEICR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 300 MFIQEKLADY--KVSLREVCGAGepLNPEVIDQVNAAWG-ATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPG-YVVR 375
Cdd:cd05939 206 YLLAQPPSEEeqKHNVRLAVGNG--LRPQIWEQFVRRFGiPQIGEFYGATEGNSSLVNIDNHVGACGFNSRILPSvYPIR 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 376 -----------ITDADGH-----PAKEGEITLALGDARPAGLMQGYQGEGGKLSGAEGDIYRSGDVAFA-------DDDG 432
Cdd:cd05939 284 likvdedtgelIRDSDGLcipcqPGEPGLLVGKIIQNDPLRRFDGYVNEGATNKKIARDVFKKGDSAFLsgdvlvmDELG 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 433 YLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV----VPSPDAiklaipKAYVLLTADAERSRDtaLSIF-RHM 507
Cdd:cd05939 364 YLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVygveVPGVEG------RAGMAAIVDPERKVD--LDRFsAVL 435
|
490 500 510
....*....|....*....|....*....|....*....
gi 91684220 508 QQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLEHDD 546
Cdd:cd05939 436 AKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQKEGYDP 474
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
74-539 |
8.85e-22 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 98.70 E-value: 8.85e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 74 MSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAK 153
Cdd:cd17656 19 LNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVLTQRHLKSK 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 154 FADLGGDSLIRivvgspqdgwrgYDEASQQSDSfTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLG 233
Cdd:cd17656 99 LSFNKSTILLE------------DPSISQEDTS-NIDYINNSDDLLYIIYTSGTTGKPKGVQLEHKNMVNLLHFEREKTN 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 234 LQPGDVHLNISSPGWAkhawSCF---FAPWNAGATVFVV-NQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLA-- 307
Cdd:cd17656 166 INFSDKVLQFATCSFD----VCYqeiFSTLLSGGTLYIIrEETKRDVEQLFDLVKRHNIEVVFLPVAFLKFIFSEREFin 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 308 DYKVSLREVCGAGEPL--NPEVIDQVNAAwGATIRDGYGQTET--TAMVGNSPGQPV-KIGSMGRPLPGYVVRITDADGH 382
Cdd:cd17656 242 RFPTCVKHIITAGEQLviTNEFKEMLHEH-NVHLHNHYGPSEThvVTTYTINPEAEIpELPPIGKPISNTWIYILDQEQQ 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 383 PAKE---GEITLAlgdarPAGLMQGYQGEgGKLSG---------AEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYR 450
Cdd:cd17656 321 LQPQgivGELYIS-----GASVARGYLNR-QELTAekffpdpfdPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYR 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 451 ISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadAERSRDTalSIFRH-MQQRLAPFKRIRKLELVTELPKT 529
Cdd:cd17656 395 IELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV----MEQELNI--SQLREyLAKQLPEYMIPSFFVPLDQLPLT 468
|
490
....*....|
gi 91684220 530 ISGKIRRVHL 539
Cdd:cd17656 469 PNGKVDRKAL 478
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
70-536 |
1.16e-21 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 100.24 E-value: 1.16e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRL-----DRGaakaV 144
Cdd:PRK05691 2215 SYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVP----LDPEYPLERLhymieDSG----I 2286
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VAAPDQVAKFADLGG--DSLIRIVVGSPQDGWRGYDEASqqsdsftPDGPTQADDPMLLYFTSGTTAKPK--LVRHSQRS 220
Cdd:PRK05691 2287 GLLLSDRALFEALGElpAGVARWCLEDDAAALAAYSDAP-------LPFLSLPQHQAYLIYTSGSTGKPKgvVVSHGEIA 2359
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 221 YPVGALSTMFwlGLQPGDVHLNISSPGWaKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRM 300
Cdd:PRK05691 2360 MHCQAVIERF--GMRADDCELHFYSINF-DAASERLLVPLLCGARVVLRAQGQWGAEEICQLIREQQVSILGFTPSYGSQ 2436
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 301 FIQEKLADYK-VSLREVCGAGEPLNPEVIDQVNAAWG-ATIRDGYGQTETTAMVGNSP-GQPVKIGS----MGRPLPGYV 373
Cdd:PRK05691 2437 LAQWLAGQGEqLPVRMCITGGEALTGEHLQRIRQAFApQLFFNAYGPTETVVMPLACLaPEQLEEGAasvpIGRVVGARV 2516
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 374 VRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGEGG---------KLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDV 443
Cdd:PRK05691 2517 AYILDADLALVPQGATgELYVGGA---GLAQGYHDRPGltaerfvadPFAADGGRLYRTGDLVRLRADGLVEYVGRIDHQ 2593
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 444 FKSSDYRISPFELESILLEHEAVAEAAV--VPSPDAIKLAipkAYvLLTADAERSrDTALSIFR-----HMQQRLAPFKR 516
Cdd:PRK05691 2594 VKIRGFRIELGEIESRLLEHPAVREAVVlaLDTPSGKQLA---GY-LVSAVAGQD-DEAQAALRealkaHLKQQLPDYMV 2668
|
490 500
....*....|....*....|
gi 91684220 517 IRKLELVTELPKTISGKIRR 536
Cdd:PRK05691 2669 PAHLILLDSLPLTANGKLDR 2688
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
201-540 |
2.77e-21 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 97.61 E-value: 2.77e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 201 LYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLnISSPGWAKHAWsCFfaPWNAGA---TVFVVNQprFDAK 277
Cdd:PLN02479 200 LGYTSGTTASPKGVVLHHRGAYLMALSNALIWGMNEGAVYL-WTLPMFHCNGW-CF--TWTLAAlcgTNICLRQ--VTAK 273
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 278 SLLATIGRCGVTTLCAPPTVWRMFIQEKLADYKVSLREV-----CGAGEPlnPEVIDQVNAAwGATIRDGYGQTET---T 349
Cdd:PLN02479 274 AIYSAIANYGVTHFCAAPVVLNTIVNAPKSETILPLPRVvhvmtAGAAPP--PSVLFAMSEK-GFRVTHTYGLSETygpS 350
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 350 AMVGNSPG---------------QPVKIGSM----------GRPLPgyvvritdADGhpAKEGEITLalgdaRPAGLMQG 404
Cdd:PLN02479 351 TVCAWKPEwdslppeeqarlnarQGVRYIGLegldvvdtktMKPVP--------ADG--KTMGEIVM-----RGNMVMKG 415
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 405 Y-QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIP 483
Cdd:PLN02479 416 YlKNPKANEEAFANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESP 495
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 91684220 484 KAYVLLTADAERSRDTALS--IFRHMQQRLaPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PLN02479 496 CAFVTLKPGVDKSDEAALAedIMKFCRERL-PAYWVPKSVVFGPLPKTATGKIQKHVLR 553
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
85-536 |
4.08e-21 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 97.11 E-value: 4.08e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 85 LRAQGLKRGDHLLLLlGNVVPLW-ETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAA-PDQVAKFADLGGD-S 161
Cdd:cd17641 28 LLALGVGRGDVVAIL-GDNRPEWvWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAEdEEQVDKLLEIADRiP 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 162 LIRIVVGSPQDGWRGYD-----------EASQQSDSFTPD------GPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVG 224
Cdd:cd17641 107 SVRYVIYCDPRGMRKYDdprlisfedvvALGRALDRRDPGlyerevAAGKGEDVAVLCTTSGTTGKPKLAMLSHGNFLGH 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 225 ALSTMFWLGLQPGDVHLnisspgwakhawSCFFAPWnAGATVFVVNQP---RF------DAKSLLATIGRCGVTTLCAPP 295
Cdd:cd17641 187 CAAYLAADPLGPGDEYV------------SVLPLPW-IGEQMYSVGQAlvcGFivnfpeEPETMMEDLREIGPTFVLLPP 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 296 TVW-------------------RMF-------------------------IQEKLADYKV--SLREVCG---------AG 320
Cdd:cd17641 254 RVWegiaadvrarmmdatpfkrFMFelgmklglraldrgkrgrpvslwlrLASWLADALLfrPLRDRLGfsrlrsaatGG 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 321 EPLNPEVIDQVNAAwGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRItdadghpAKEGEITlalgdARPAG 400
Cdd:cd17641 334 AALGPDTFRFFHAI-GVPLKQLYGQTELAGAYTVHRDGDVDPDTVGVPFPGTEVRI-------DEVGEIL-----VRSPG 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 401 LMQGY--QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSD-YRISPFELESILLEHEAVAEAAVV----P 473
Cdd:cd17641 401 VFVGYykNPEATAEDFDEDGWLHTGDAGYFKENGHLVVIDRAKDVGTTSDgTRFSPQFIENKLKFSPYIAEAVVLgagrP 480
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 474 SPDA---IKLAI---------------------PKAYVLLTADAERsrdtalsifrhMQQRLAPFKRIRKLELvteLPK- 528
Cdd:cd17641 481 YLTAficIDYAIvgkwaeqrgiafttytdlasrPEVYELIRKEVEK-----------VNASLPEAQRIRRFLL---LYKe 546
|
570
....*....|....*.
gi 91684220 529 --------TISGKIRR 536
Cdd:cd17641 547 ldaddgelTRTRKVRR 562
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
70-539 |
1.14e-20 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 94.84 E-value: 1.14e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDELRDRLdrgaakavvaapd 149
Cdd:cd17650 14 TYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVP----IDPDYPAERL------------- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakfADLGGDSLIRIVVgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:cd17650 77 -----QYMLEDSGAKLLL-------------------------TQPEDLAYVIYTSGTTGKPKGVMVEHRNVAHAAHAWR 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDV-HLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFI------ 302
Cdd:cd17650 127 REYELDSFPVrLLQMASFSFDVFAGDFARSLLNGGTLVICPDEVKLDPAALYDLILKSRITLMESTPALIRPVMayvyrn 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 QEKLADYKVSL--REVCGAGEPlnPEVIDQVNAawGATIRDGYGQTETT--------AMVGNSPGQPVKIGsmgRPLPGY 372
Cdd:cd17650 207 GLDLSAMRLLIvgSDGCKAQDF--KTLAARFGQ--GMRIINSYGVTEATidstyyeeGRDPLGDSANVPIG---RPLPNT 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 373 VVRITDADGHPAKEGEI-TLALGDArpaGLMQGYQGE--------GGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDV 443
Cdd:cd17650 280 AMYVLDERLQPQPVGVAgELYIGGA---GVARGYLNRpeltaerfVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQ 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 444 FKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadAERSRDTAlSIFRHMQQRLAPFKRIRKLELV 523
Cdd:cd17650 357 VKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVV----AAATLNTA-ELRAFLAKELPSYMIPSYYVQL 431
|
490
....*....|....*.
gi 91684220 524 TELPKTISGKIRRVHL 539
Cdd:cd17650 432 DALPLTPNGKVDRRAL 447
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
70-536 |
1.48e-20 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 94.54 E-value: 1.48e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPattlLTPDelrdrldrgaakavvaAPD 149
Cdd:cd17645 25 TYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVP----IDPD----------------YPG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QvaKFADLGGDSLIRIVVgspqdgwrgydeasqqsdsftpdgpTQADDPMLLYFTSGTTAKPKLVRHSQRSYpvgaLSTM 229
Cdd:cd17645 85 E--RIAYMLADSSAKILL-------------------------TNPDDLAYVIYTSGSTGLPKGVMIEHHNL----VNLC 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FW----LGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVVNQP-RFDAKSLLATIGRCGVTTLCAPPTVWRMFIQE 304
Cdd:cd17645 134 EWhrpyFGVTPADKSLVYASFSFDASAWE-IFPHLTAGAALHVVPSErRLDLDALNDYFNQEGITISFLPTGAAEQFMQL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 305 KladyKVSLREVCGAGEPLNPEVIDqvnaawGATIRDGYGQTETTAMVGNSPGQPVKIG-SMGRPLPGYVVRITDADGHP 383
Cdd:cd17645 213 D----NQSLRVLLTGGDKLKKIERK------GYKLVNNYGPTENTVVATSFEIDKPYANiPIGKPIDNTRVYILDEALQL 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 384 AKEG---EITLAlGDarpaGLMQGYQGE---------GGKLSGAEgDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRI 451
Cdd:cd17645 283 QPIGvagELCIA-GE----GLARGYLNRpeltaekfiVHPFVPGE-RMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRI 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 452 SPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLL--TADAERSRDTalsifrhMQQRLAPFKRIRKLELVTELPKT 529
Cdd:cd17645 357 EPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYVTApeEIPHEELREW-------LKNDLPDYMIPTYFVHLKALPLT 429
|
....*..
gi 91684220 530 ISGKIRR 536
Cdd:cd17645 430 ANGKVDR 436
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
78-540 |
2.07e-20 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 94.94 E-value: 2.07e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 78 SNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQVAKFAD 156
Cdd:PRK08751 60 VEQFAAYLLGElQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQ 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 157 LGGDSLIRIVVGSPQDGWRGYDEAS------QQSDSFTPDG--------------------PTQ---ADDPMLLYFTSGT 207
Cdd:PRK08751 140 VIADTPVKQVITTGLGDMLGFPKAAlvnfvvKYVKKLVPEYringairfrealalgrkhsmPTLqiePDDIAFLQYTGGT 219
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 208 TAKPKLVRHSQRSYPVGALSTMFWLG----LQPGDVHLNISSPGWAKHAWScffapwnAGATVFV--------VNQPRfD 275
Cdd:PRK08751 220 TGVAKGAMLTHRNLVANMQQAHQWLAgtgkLEEGCEVVITALPLYHIFALT-------ANGLVFMkiggcnhlISNPR-D 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 276 AKSLLATIGRCGVTTLCAPPTVWRM------FIQEKLADYKVSLrevcGAGEPLNPEVIDQVNAAWGATIRDGYGQTETT 349
Cdd:PRK08751 292 MPGFVKELKKTRFTAFTGVNTLFNGllntpgFDQIDFSSLKMTL----GGGMAVQRSVAERWKQVTGLTLVEAYGLTETS 367
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 350 -AMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPAKEGEItlalGDARPAG--LMQGY---QGEGGKLSGAEGdIYRSG 423
Cdd:PRK08751 368 pAACINPLTLKEYNGSIGLPIPSTDACIKDDAGTVLAIGEI----GELCIKGpqVMKGYwkrPEETAKVMDADG-WLHTG 442
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 424 DVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPD-----AIKLAIPKAYVLLTADAERSrd 498
Cdd:PRK08751 443 DIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDeksgeIVKVVIVKKDPALTAEDVKA-- 520
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 91684220 499 talsifrHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK08751 521 -------HARANLTGYKQPRIIEFRKELPKTNVGKILRRELR 555
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
70-472 |
2.35e-20 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 94.06 E-value: 2.35e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIpattLLTPdelrdRLDRGAAKAVvaapd 149
Cdd:cd05910 4 SFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPV----LIDP-----GMGRKNLKQC----- 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 qvakFADLGGDSLIrivvgspqdgwrgydeasqqsdsftpdGPTQADDPMLLYFTSGTTAKPKLV--RHSQRSYPVGALS 227
Cdd:cd05910 70 ----LQEAEPDAFI---------------------------GIPKADEPAAILFTSGSTGTPKGVvyRHGTFAAQIDALR 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 228 TMFwlGLQPGDVHLnissPGWAKHAwscFFAPWNAGATVFVVNQP----RFDAKSLLATIGRCGVTTLCAPPTVWrmfiq 303
Cdd:cd05910 119 QLY--GIRPGEVDL----ATFPLFA---LFGPALGLTSVIPDMDPtrpaRADPQKLVGAIRQYGVSIVFGSPALL----- 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 EKLADYKV-------SLREVCGAGEPLNPEVIDQVNAAW--GATIRDGYGQTET--TAMVGNS--------PGQPVKIGS 364
Cdd:cd05910 185 ERVARYCAqhgitlpSLRRVLSAGAPVPIALAARLRKMLsdEAEILTPYGATEAlpVSSIGSRellatttaATSGGAGTC 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 365 MGRPLPGYVVRITDADGHPAKE------------GEITLALGDARPAGLMQGYQGEGGKLS-GAEGDIYRSGDVAFADDD 431
Cdd:cd05910 265 VGRPIPGVRVRIIEIDDEPIAEwddtlelprgeiGEITVTGPTVTPTYVNRPVATALAKIDdNSEGFWHRMGDLGYLDDE 344
|
410 420 430 440
....*....|....*....|....*....|....*....|.
gi 91684220 432 GYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVV 472
Cdd:cd05910 345 GRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALV 385
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
77-541 |
1.52e-19 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 92.11 E-value: 1.52e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 77 RSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAApdqvAKFAD 156
Cdd:cd05915 33 RARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLLFD----PNLLP 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 157 LGGDSL--IRIVVGSP--QDGWRGYDE-ASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGA--LSTM 229
Cdd:cd05915 109 LVEAIRgeLKTVQHFVvmDEKAPEGYLaYEEALGEEADPVRVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSlaASLV 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHLnISSPGWAKHAWsCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLA-- 307
Cdd:cd05915 189 DGTALSEKDVVL-PVVPMFHVNAW-CLPYAATLVGAKQVLPGPRLDPASLVELFDGEGVTFTAGVPTVWLALADYLEStg 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 308 -DYKVSLREVCGAGEPlnPEVIDQVNAAWGATIRDGYGQTETTAMvgnspGQPVKIGSMGRPLPGY-VVRITDADG--HP 383
Cdd:cd05915 267 hRLKTLRRLVVGGSAA--PRSLIARFERMGVEVRQGYGLTETSPV-----VVQNFVKSHLESLSEEeKLTLKAKTGlpIP 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 384 AKEGEI-------------TLALGDARPAGLMQGY--QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSD 448
Cdd:cd05915 340 LVRLRVadeegrpvpkdgkALGEVQLKGPWITGGYygNEEATRSALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGG 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 449 YRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDtalsIFRHMQQRLAPFKRIRKLELVT-ELP 527
Cdd:cd05915 420 EWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPEE----LNEHLLKAGFAKWQLPDAYVFAeEIP 495
|
490
....*....|....
gi 91684220 528 KTISGKIRRVHLRR 541
Cdd:cd05915 496 RTSAGKFLKRALRE 509
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
455-533 |
1.57e-19 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 82.98 E-value: 1.57e-19
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 91684220 455 ELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAErsrDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGK 533
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVE---LLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
29-540 |
1.70e-19 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 92.04 E-value: 1.70e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 29 DPAPFNWALDWFDAELATNADsrdraalwivdgitgvelKPSFATMSK---------RSNQVANYLRAQ-GLKRGDHLLL 98
Cdd:PRK08974 18 NPDRYQSLVDMFEQAVARYAD------------------QPAFINMGEvmtfrkleeRSRAFAAYLQNGlGLKKGDRVAL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 99 LLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAapdqVAKFA---------------------DL 157
Cdd:PRK08974 80 MMPNLLQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVI----VSNFAhtlekvvfktpvkhviltrmgDQ 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 158 ---GGDSLIRIVVGSPQDGWRGYD--------EASQ---QSDSFTPDgpTQADDPMLLYFTSGTTAKPKlvrhsqrsypv 223
Cdd:PRK08974 156 lstAKGTLVNFVVKYIKRLVPKYHlpdaisfrSALHkgrRMQYVKPE--LVPEDLAFLQYTGGTTGVAK----------- 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 GALSTmfwlglqpgdvHLN-ISSPGWAKHAWSCFFAPWNA------------------------GATVFVVNQPRfDAKS 278
Cdd:PRK08974 223 GAMLT-----------HRNmLANLEQAKAAYGPLLHPGKElvvtalplyhifaltvncllfielGGQNLLITNPR-DIPG 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 279 LLATIGRCGVTTLCAPPTVWRMFIQEKlaDYK----VSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGN 354
Cdd:PRK08974 291 FVKELKKYPFTAITGVNTLFNALLNNE--EFQeldfSSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSV 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 355 SPGQPVKI-GSMGRPLPGYVVRITDADGHPAKEGEItlalGD--ARPAGLMQGY--QGEGGKLSGAEGDIyRSGDVAFAD 429
Cdd:PRK08974 369 NPYDLDYYsGSIGLPVPSTEIKLVDDDGNEVPPGEP----GElwVKGPQVMLGYwqRPEATDEVIKDGWL-ATGDIAVMD 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 430 DDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLltadaerSRDTALS---IFRH 506
Cdd:PRK08974 444 EEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVV-------KKDPSLTeeeLITH 516
|
570 580 590
....*....|....*....|....*....|....
gi 91684220 507 MQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK08974 517 CRRHLTGYKVPKLVEFRDELPKSNVGKILRRELR 550
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
79-539 |
8.71e-19 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 89.68 E-value: 8.71e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 79 NQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDqvakfADLG 158
Cdd:PRK05857 52 GGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQITDPAAALVAPG-----SKMA 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 159 GDSLIRIVVGSPQDGWRGYDEASQQSDSFTPDGPTQ-----ADDPMLLYFTSGTTAKPKLVRHSQRSY----PVGALSTM 229
Cdd:PRK05857 127 SSAVPEALHSIPVIAVDIAAVTRESEHSLDAASLAGnadqgSEDPLAMIFTSGTTGEPKAVLLANRTFfavpDILQKEGL 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVhlnISSPGWAKHA---WSCFFAPWNAGATVFVVNQprfdAKSLLATIGRCGVTTLCAPPTVWRMFIQE-K 305
Cdd:PRK05857 207 NWVTWVVGET---TYSPLPATHIgglWWILTCLMHGGLCVTGGEN----TTSLLEILTTNAVATTCLVPTLLSKLVSElK 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 LADYKV-SLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTET--TAM-VGNSPGQPVKI--GSMGRPLPGYVVRITDA 379
Cdd:PRK05857 280 SANATVpSLRLVGYGGSRAIAADVRFIEAT-GVRTAQVYGLSETgcTALcLPTDDGSIVKIeaGAVGRPYPGVDVYLAAT 358
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 380 DG-HPAKEGEITLA----LGDARPAGlMQGYQGEGGKLSG--AEGDIyRSGDVAFADDDGYLTFVGRTDDVFKSSDYRIS 452
Cdd:PRK05857 359 DGiGPTAPGAGPSAsfgtLWIKSPAN-MLGYWNNPERTAEvlIDGWV-NTGDLLERREDGFFYIKGRSSEMIICGGVNIA 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 453 PFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSRDTAL--SIFRHMQQRLAPFKRIRKLELVTELPKTI 530
Cdd:PRK05857 437 PDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVVASAELDESAARALkhTIAARFRRESEPMARPSTIVIVTDIPRTQ 516
|
....*....
gi 91684220 531 SGKIRRVHL 539
Cdd:PRK05857 517 SGKVMRASL 525
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
64-540 |
9.63e-19 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 89.50 E-value: 9.63e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 64 GVELkpSFATMSKRSNQVANYLRAQ-GLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAK 142
Cdd:PRK12492 47 GVTL--SYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGAR 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 143 AVV------AAPDQVakFADLGGDSLIRIVVG---SPQDGWR---------------------GYDEASQQSDSFTPDGP 192
Cdd:PRK12492 125 ALVylnmfgKLVQEV--LPDTGIEYLIEAKMGdllPAAKGWLvntvvdkvkkmvpayhlpqavPFKQALRQGRGLSLKPV 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 193 TQA-DDPMLLYFTSGTTAKPK--------LVRHSQRSYpvgalSTMFWLG------LQPGDVHLNISSPGWAKHAWS--C 255
Cdd:PRK12492 203 PVGlDDIAVLQYTGGTTGLAKgamlthgnLVANMLQVR-----ACLSQLGpdgqplMKEGQEVMIAPLPLYHIYAFTanC 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 256 FFAPWNAGATVFVVNqPRfDAKSLLATIGRCGVTTLCAPPTVWRMFIQE---KLADYKvSLREVCGAGEPLNPEVIDQVN 332
Cdd:PRK12492 278 MCMMVSGNHNVLITN-PR-DIPGFIKELGKWRFSALLGLNTLFVALMDHpgfKDLDFS-ALKLTNSGGTALVKATAERWE 354
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 333 AAWGATIRDGYGQTETTAMVGNSP-GQPVKIGSMGRPLPGYVVRITDADGH--PAKE-GEITLalgdaRPAGLMQGY--Q 406
Cdd:PRK12492 355 QLTGCTIVEGYGLTETSPVASTNPyGELARLGTVGIPVPGTALKVIDDDGNelPLGErGELCI-----KGPQVMKGYwqQ 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 407 GEG-GKLSGAEGdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKA 485
Cdd:PRK12492 430 PEAtAEALDAEG-WFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKL 508
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 91684220 486 YVLltadaerSRDTALSIFR---HMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:PRK12492 509 FVV-------ARDPGLSVEElkaYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELR 559
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
192-536 |
1.04e-18 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 89.80 E-value: 1.04e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 192 PTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALstMFWLGLQPGD---VHLNISSPGWAK-HawSCFFAPWNAGATvF 267
Cdd:PTZ00237 250 PVESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLK--YYWRSIIEKDiptVVFSHSSIGWVSfH--GFLYGSLSLGNT-F 324
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 268 V-----VNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQ-----EKL-ADYKVS-LREVCGAGEPLNPEVIDQVNAAW 335
Cdd:PTZ00237 325 VmfeggIIKNKHIEDDLWNTIEKHKVTHTLTLPKTIRYLIKtdpeaTIIrSKYDLSnLKEIWCGGEVIEESIPEYIENKL 404
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 336 GATIRDGYGQTETTAMVGNSPGQPVK-IGSMGRPLPGYVVRITDADGH--PAKE-GEITLAL------------GDARPA 399
Cdd:PTZ00237 405 KIKSSRGYGQTEIGITYLYCYGHINIpYNATGVPSIFIKPSILSEDGKelNVNEiGEVAFKLpmppsfattfykNDEKFK 484
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 400 GLMQGYQGeggklsgaegdIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIK 479
Cdd:PTZ00237 485 QLFSKFPG-----------YYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDC 553
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 91684220 480 LAIPKAYVLLTADAERSR----DTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:PTZ00237 554 YNVPIGLLVLKQDQSNQSidlnKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPR 614
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
197-561 |
1.20e-18 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 89.31 E-value: 1.20e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 197 DPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMF-W-LGLQPgdVHLnISSPGWAKHAWSCFFAPWNAGATVFVVNQprF 274
Cdd:PLN03102 187 DPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIgWeMGTCP--VYL-WTLPMFHCNGWTFTWGTAARGGTSVCMRH--V 261
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 275 DAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLAD--YKVSLREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMV 352
Cdd:PLN03102 262 TAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDlsPRSGPVHVLTGGSPPPAALVKKVQRL-GFQVMHAYGLTEATGPV 340
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 ---------GNSP-GQPVKI----GSMGRPLPGYVVRITDADGHPAKEGEiTLALGDARPAGLMQGYQGEGGKLSGA-EG 417
Cdd:PLN03102 341 lfcewqdewNRLPeNQQMELkarqGVSILGLADVDVKNKETQESVPRDGK-TMGEIVIKGSSIMKGYLKNPKATSEAfKH 419
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 418 DIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERSR 497
Cdd:PLN03102 420 GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFVVLEKGETTKE 499
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 91684220 498 DTALS-------IFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRrlehdddraDTLRGVEYREED 561
Cdd:PLN03102 500 DRVDKlvtrerdLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLR---------DIAKGLVVEDED 561
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
48-542 |
1.39e-18 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 89.22 E-value: 1.39e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 48 ADSRDRAALWIVDGITGVELKPSFATMSKRSNQVANYLRAQGlKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPA---T 124
Cdd:cd05931 4 AARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLpppT 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 125 TLLTPDELRDRLDRGAAKAVVAAPDQVAKFADLggdslIRIVVGSPQDGWRGYDE-ASQQSDSFTPDGPTqADDPMLLYF 203
Cdd:cd05931 83 PGRHAERLAAILADAGPRVVLTTAAALAAVRAF-----AASRPAAGTPRLLVVDLlPDTSAADWPPPSPD-PDDIAYLQY 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 204 TSGTTAKPK--LVRH---------SQRSYPVGALSTM-FWL------GLqpgdvHLNIsspgwakhawscfFAPWNAGAT 265
Cdd:cd05931 157 TSGSTGTPKgvVVTHrnllanvrqIRRAYGLDPGDVVvSWLplyhdmGL-----IGGL-------------LTPLYSGGP 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 266 VFVVNQPRFDAKSL--LATIGRCGVTT---------LCApptvwRMFIQEKLADYKVS-LREVCGAGEPLNPEVIDQVNA 333
Cdd:cd05931 219 SVLMSPAAFLRRPLrwLRLISRYRATIsaapnfaydLCV-----RRVRDEDLEGLDLSsWRVALNGAEPVRPATLRRFAE 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 334 AWGA------TIRDGYGQTETTAMV-GNSPGQP-------------------------VKIGSMGRPLPGYVVRITDADG 381
Cdd:cd05931 294 AFAPfgfrpeAFRPSYGLAEATLFVsGGPPGTGpvvlrvdrdalagravavaaddpaaRELVSCGRPLPDQEVRIVDPET 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 382 H----PAKEGEITLAlGDArpagLMQGYQGE--------GGKLSGAEGDIYRSGDVAFADDdGYLTFVGRTDDVFKSSDY 449
Cdd:cd05931 374 GrelpDGEVGEIWVR-GPS----VASGYWGRpeataetfGALAATDEGGWLRTGDLGFLHD-GELYITGRLKDLIIVRGR 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 450 RISPFELE-SILLEHEAVAEAAVVpspdAIKLAIPKAYVLLTADAERSRDTAL---SIFRHMQQRLAPFKRIRKLELVT- 524
Cdd:cd05931 448 NHYPQDIEaTAEEAHPALRPGCVA----AFSVPDDGEERLVVVAEVERGADPAdlaAIAAAIRAAVAREHGVAPADVVLv 523
|
570 580
....*....|....*....|.
gi 91684220 525 ---ELPKTISGKIRRVHLRRL 542
Cdd:cd05931 524 rpgSIPRTSSGKIQRRACRAA 544
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
191-534 |
4.75e-18 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 88.44 E-value: 4.75e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 191 GPTQADDPMLLYFTSGTTAKPKLVRHSQRSY--PVGALSTMFwlGLQPGDVHLNISSPgwaKHAWS---CFFAPWNAG-A 264
Cdd:PRK08633 777 PTFKPDDTATIIFSSGSEGEPKGVMLSHHNIlsNIEQISDVF--NLRNDDVILSSLPF---FHSFGltvTLWLPLLEGiK 851
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 265 TVFVVNqPrFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLA---DYKvSLREV-CGAgEPLNPEVIDQVNAAWGATIR 340
Cdd:PRK08633 852 VVYHPD-P-TDALGIAKLVAKHRATILLGTPTFLRLYLRNKKLhplMFA-SLRLVvAGA-EKLKPEVADAFEEKFGIRIL 927
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 341 DGYGQTETTAMVG-NSP-----------GQpvKIGSMGRPLPGYVVRITDAD-GHPAKEGEITLALgdARPAGLMQGYQG 407
Cdd:PRK08633 928 EGYGATETSPVASvNLPdvlaadfkrqtGS--KEGSVGMPLPGVAVRIVDPEtFEELPPGEDGLIL--IGGPQVMKGYLG 1003
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 408 EGGKLSGAEGDI-----YRSGDVAFADDDGYLTFVGrtddvfkssdyRISPF-----ELESILLEHEAVAEA-------- 469
Cdd:PRK08633 1004 DPEKTAEVIKDIdgigwYVTGDKGHLDEDGFLTITD-----------RYSRFakiggEMVPLGAVEEELAKAlggeevvf 1072
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 470 AVVPSPDAIKlaiPKAYVLLTADAERSRDTalsIFRHM-QQRLAPFKRIRKLELVTELPKTISGKI 534
Cdd:PRK08633 1073 AVTAVPDEKK---GEKLVVLHTCGAEDVEE---LKRAIkESGLPNLWKPSRYFKVEALPLLGSGKL 1132
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
195-533 |
5.78e-18 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 85.90 E-value: 5.78e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 195 ADDPMLLYfTSGTTAKPKLVRHSQRSYPVGALS---------TMFWLGLQ-----PGDVHLNISSPGWAKHAWSCFFAPw 260
Cdd:cd05924 3 ADDLYILY-TGGTTGMPKGVMWRQEDIFRMLMGgadfgtgefTPSEDAHKaaaaaAGTVMFPAPPLMHGTGSWTAFGGL- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 261 NAGATVFVVNqPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYK----VSLREVCGAGEPLNPEVIDQVNAAW- 335
Cdd:cd05924 81 LGGQTVVLPD-DRFDPEEVWRTIEKHKVTSMTIVGDAMARPLIDALRDAGpydlSSLFAISSGGALLSPEVKQGLLELVp 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 336 GATIRDGYGQTETTA-MVGNSPGQPVKIGSMGRPLPGYVVriTDADGHPAKEGEitLALGDARPAGLM-QGYQGEGGKLS 413
Cdd:cd05924 160 NITLVDAFGSSETGFtGSGHSAGSGPETGPFTRANPDTVV--LDDDGRVVPPGS--GGVGWIARRGHIpLGYYGDEAKTA 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 414 G----AEGDIYR-SGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVL 488
Cdd:cd05924 236 EtfpeVDGVRYAvPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAVVQ 315
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 91684220 489 LTADAERSRDtalSIFRHMQQRLAPFKRIRKLELVTELPKTISGK 533
Cdd:cd05924 316 LREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
193-536 |
1.33e-17 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 85.56 E-value: 1.33e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 193 TQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHAWScFFAPWNAGATVFVV-NQ 271
Cdd:cd17644 103 TQPENLAYVIYTSGSTGKPKGVMIEHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEE-IYVTLLSGATLVLRpEE 181
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 PRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQE---KLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIR--DGYGQT 346
Cdd:cd17644 182 MRSSLEDFVQYIQQWQLTVLSLPPAYWHLLVLElllSTIDLPSSLRLVIVGGEAVQPELVRQWQKNVGNFIQliNVYGPT 261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 347 E-TTAMVGNSPGQPVKIGS----MGRPLPGYVVRITDADGHPAK---EGEITLAlgdarPAGLMQGY------------- 405
Cdd:cd17644 262 EaTIAATVCRLTQLTERNItsvpIGRPIANTQVYILDENLQPVPvgvPGELHIG-----GVGLARGYlnrpeltaekfis 336
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 406 ----QGEGGKLsgaegdiYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLA 481
Cdd:cd17644 337 hpfnSSESERL-------YKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNK 409
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 482 IPKAYVLltADAERSRDTAlSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:cd17644 410 RLVAYIV--PHYEESPSTV-ELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDR 461
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
203-471 |
2.03e-16 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 81.75 E-value: 2.03e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 203 FTSGTTAKPKLVR---HSQRSYPVGALSTMfwlGLQPGDVHLnISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSL 279
Cdd:cd17654 125 HTSGTTGTPKIVAvphKCILPNIQHFRSLF---NITSEDILF-LTSPLTFDPSVVEIFLSLSSGATLLIVPTSVKVLPSK 200
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 280 LATI--GRCGVTTLCAPPTVWRMF----IQEKLADYKVSLREVCGAGEPLnpeVIDQVNAAW-----GATIRDGYGQTET 348
Cdd:cd17654 201 LADIlfKRHRITVLQATPTLFRRFgsqsIKSTVLSATSSLRVLALGGEPF---PSLVILSSWrgkgnRTRIFNIYGITEV 277
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 349 TA-------MVGNSPgqpVKIGSmgrPLPGYVVRITDADGHpakEGEITLALGDARPAGLMQGYqgeggkLSGAEGDIYR 421
Cdd:cd17654 278 SCwalaykvPEEDSP---VQLGS---PLLGTVIEVRDQNGS---EGTGQVFLGGLNRVCILDDE------VTVPKGTMRA 342
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|
gi 91684220 422 SGDVAfADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV 471
Cdd:cd17654 343 TGDFV-TVKDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLGVESCAV 391
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
70-543 |
1.08e-15 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 80.98 E-value: 1.08e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPD 149
Cdd:PRK05691 3747 SYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQRLQRIIELSRTPVLVCSAA 3826
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAK----FADLGGDSLIRIVVgspqdgWrgydEASQQSD--SFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPV 223
Cdd:PRK05691 3827 CREQaralLDELGCANRPRLLV------W----EEVQAGEvaSHNPGIYSGPDNLAYVIYTSGSTGLPKGVMVEQRGMLN 3896
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 GALSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVwrmfIQ 303
Cdd:PRK05691 3897 NQLSKVPYLALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIVPNAIAHDPQGLLAHVQAQGITVLESVPSL----IQ 3972
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 304 EKLADYKVS---LREVCGAGEPLNPEVIDQ----------VNAawgatirdgYGQTETTAMVG-------NSPGQPVKIG 363
Cdd:PRK05691 3973 GMLAEDRQAldgLRWMLPTGEAMPPELARQwlqrypqiglVNA---------YGPAECSDDVAffrvdlaSTRGSYLPIG 4043
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 364 SmgrPLPGYVVRITDADGH--PAKE-GEITLAlgdarPAGLMQGYQGEGGKLS--------GAEGD-IYRSGDVAFADDD 431
Cdd:PRK05691 4044 S---PTDNNRLYLLDEALElvPLGAvGELCVA-----GTGVGRGYVGDPLRTAlafvphpfGAPGErLYRTGDLARRRSD 4115
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 432 GYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV--VPSPDAIKLAipkAYvLLTADAERSRDTALSIFR-HMQ 508
Cdd:PRK05691 4116 GVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVavQEGVNGKHLV---GY-LVPHQTVLAQGALLERIKqRLR 4191
|
490 500 510
....*....|....*....|....*....|....*
gi 91684220 509 QRLAPFKRIRKLELVTELPKTISGKIRRVHLRRLE 543
Cdd:PRK05691 4192 AELPDYMVPLHWLWLDRLPLNANGKLDRKALPALD 4226
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
111-540 |
1.51e-15 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 79.47 E-value: 1.51e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 111 LAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV--------------------AAPDQVAKFADLGGDSLIRIVVGSP 170
Cdd:PLN03051 12 LAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFtqdvvlrggralplyskvveAAPAKAIVLPAAGEPVAVPLREQDL 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 171 QdgWRGYDEASQQSDSFTPDGPT----QADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSP 246
Cdd:PLN03051 92 S--WCDFLGVAAAQGSVGGNEYSpvyaPVESVTNILFSSGTTGEPKAIPWTHLSPLRCASDGWAHMDIQPGDVVCWPTNL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 247 GWAKHAWsCFFAPWNAGATVFVVNQPRFdAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYK----VSLREVCGAGEP 322
Cdd:PLN03051 170 GWMMGPW-LLYSAFLNGATLALYGGAPL-GRGFGKFVQDAGVTVLGLVPSIVKAWRHTGAFAMEgldwSKLRVFASTGEA 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 323 LNPEvidqvNAAWGATIRDGY-------GQTE-TTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHPakEGEITLALG 394
Cdd:PLN03051 248 SAVD-----DVLWLSSVRGYYkpvieycGGTElASGYISSTLLQPQAPGAFSTASLGTRFVLLNDNGVP--YPDDQPCVG 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 395 DARPAGLMQGYQG------------EGGKLSGAEG-DIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILL 461
Cdd:PLN03051 321 EVALAPPMLGASDrllnadhdkvyyKGMPMYGSKGmPLRRHGDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIERACD 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 462 E-HEAVAEAAVVP------SPDAIKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKI 534
Cdd:PLN03051 401 RaVAGIAETAAVGvappdgGPELLVIFLVLGEEKKGFDQARPEALQKKFQEAIQTNLNPLFKVSRVKIVPELPRNASNKL 480
|
....*.
gi 91684220 535 RRVHLR 540
Cdd:PLN03051 481 LRRVLR 486
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
14-544 |
1.99e-14 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 76.16 E-value: 1.99e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 14 HRTDYAAAV-AGFRWPDPAPFNWA-LDWfdAE-LATNADSRDRAALWIVDGITGVELkpSFATMSKRSNQVANYLRAQGL 90
Cdd:cd05943 45 GSKPYDVVVvSGRIMPGARWFPGArLNY--AEnLLRHADADDPAAIYAAEDGERTEV--TWAELRRRVARLAAALRALGV 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 91 KRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAP---------DQVAKFADL--GG 159
Cdd:cd05943 121 KPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRFGQIEPKVLFAVDaytyngkrhDVREKVAELvkGL 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 160 DSLIRIVV-----GSPQDGWRGYDEASQQSD--SFTPDG-----PTQADDPMLLYFTSGTTAKPKLVRHSQrsypVGAL- 226
Cdd:cd05943 201 PSLLAVVVvpytvAAGQPDLSKIAKALTLEDflATGAAGelefePLPFDHPLYILYSSGTTGLPKCIVHGA----GGTLl 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 227 ----STMFWLGLQPGDVHLNISSPGWAKhawscffapWN-------AGATVFVVNQPRF--DAKSLLATIGRCGVTTLCA 293
Cdd:cd05943 277 qhlkEHILHCDLRPGDRLFYYTTCGWMM---------WNwlvsglaVGATIVLYDGSPFypDTNALWDLADEEGITVFGT 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 294 PPTvWRMFIQEK----LADYKVS-LREVCGAGEPLNPEVIDQVNAAWGATIRDGY--GQTE-TTAMVGNSPGQPVKIGSM 365
Cdd:cd05943 348 SAK-YLDALEKAglkpAETHDLSsLRTILSTGSPLKPESFDYVYDHIKPDVLLASisGGTDiISCFVGGNPLLPVYRGEI 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 GRPLPGYVVRITDADGHPAKE--GEitlaLGDARPAGLMQGY---QGEGGKLSGAEGDIY----RSGDVAFADDDGYLTF 436
Cdd:cd05943 427 QCRGLGMAVEAFDEEGKPVWGekGE----LVCTKPFPSMPVGfwnDPDGSRYRAAYFAKYpgvwAHGDWIEITPRGGVVI 502
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 437 VGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA---------IKLAipKAYVLLTADAERSRDTalsIFRHM 507
Cdd:cd05943 503 LGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKdgdervilfVKLR--EGVELDDELRKRIRST---IRSAL 577
|
570 580 590
....*....|....*....|....*....|....*..
gi 91684220 508 QQRLAPFKRIRklelVTELPKTISGKIRRVHLRRLEH 544
Cdd:cd05943 578 SPRHVPAKIIA----VPDIPRTLSGKKVEVAVKKIIA 610
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
70-539 |
2.46e-14 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 75.79 E-value: 2.46e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQ-GLKRGDHLLLLLGN---VVPLWetmLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVV 145
Cdd:cd05938 7 TYRDVDRRSNQAARALLAHaGLRPGDTVALLLGNepaFLWIW---LGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKVLV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 146 AAPDQVAKFADL------GGDSLIRIVVGSPQDGWRGY-DEASQQSDSFTPDGPTQA---DDPMLLYFTSGTTAKPKLVR 215
Cdd:cd05938 84 VAPELQEAVEEVlpalraDGVSVWYLSHTSNTEGVISLlDKVDAASDEPVPASLRAHvtiKSPALYIYTSGTTGLPKAAR 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 216 HSQRSypVGALSTMFWL-GLQPGDVhLNISSPGWAKHAW-----SCFfapwNAGATvfVVNQPRFDAKSLLATIGRCGVT 289
Cdd:cd05938 164 ISHLR--VLQCSGFLSLcGVTADDV-IYITLPLYHSSGFllgigGCI----ELGAT--CVLKPKFSASQFWDDCRKHNVT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 290 T----------LCAPPTvwrmfiQEKLADYKVSLrevcGAGEPLNPEVIDQVNAAWGAT-IRDGYGQTETTAMVGNSPGq 358
Cdd:cd05938 235 ViqyigellryLCNQPQ------SPNDRDHKVRL----AIGNGLRADVWREFLRRFGPIrIREFYGSTEGNIGFFNYTG- 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 359 pvKIGSMGRP-------LPGYVVR--------ITDADGH--PAKEGEITLALGDARPAGLMQGYQG-----EGGKLSGA- 415
Cdd:cd05938 304 --KIGAVGRVsylykllFPFELIKfdvekeepVRDAQGFciPVAKGEPGLLVAKITQQSPFLGYAGdkeqtEKKLLRDVf 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 416 -EGDIY-RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAV--VPSPD--------AIKLAIP 483
Cdd:cd05938 382 kKGDVYfNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQEVNVygVTVPGhegrigmaAVKLKPG 461
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 484 KAYvlltaDAERsrdtalsIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHL 539
Cdd:cd05938 462 HEF-----DGKK-------LYQHVREYLPAYARPRFLRIQDSLEITGTFKQQKVRL 505
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
70-540 |
5.40e-14 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 74.14 E-value: 5.40e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPATTLLtPDELRDRLdrgaakavvaAPD 149
Cdd:PRK09029 30 TWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQL-PQPLLEEL----------LPS 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 150 QVAKFadlggdslirIVVGSPQDGWRGYDEASQQSDSFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTM 229
Cdd:PRK09029 99 LTLDF----------ALVLEGENTFSALTSLHLQLVEGAHAVAWQPQRLATMTLTSGSTGLPKAAVHTAQAHLASAEGVL 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 230 FWLGLQPGDVHL------NISSPG--WAkhawscffapW-NAGATVFVVNQPRFDAkSLLatigrcGVTTLCAPPT-VWR 299
Cdd:PRK09029 169 SLMPFTAQDSWLlslplfHVSGQGivWR----------WlYAGATLVVRDKQPLEQ-ALA------GCTHASLVPTqLWR 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 300 MFIQEKLAdykVSLREVCGAGEPLNPEVIDQVnAAWGATIRDGYGQTETTAMV-----GNSPGqpvkigsMGRPLPGYVV 374
Cdd:PRK09029 232 LLDNRSEP---LSLKAVLLGGAAIPVELTEQA-EQQGIRCWCGYGLTEMASTVcakraDGLAG-------VGSPLPGREV 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 375 RITDadghpakeGEITLalgdaRPAGLMQGYqgeggklsgaegdiYRSGD-VAFADDDGY-------------LTFVGRT 440
Cdd:PRK09029 301 KLVD--------GEIWL-----RGASLALGY--------------WRQGQlVPLVNDEGWfatrdrgewqngeLTILGRL 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 441 DDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTADAERsrdTALSIFrhMQQRLAPFKR-IRK 519
Cdd:PRK09029 354 DNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPVADAEFGQRPVAVVESDSEAAV---VNLAEW--LQDKLARFQQpVAY 428
|
490 500
....*....|....*....|..
gi 91684220 520 LELVTELPktISG-KIRRVHLR 540
Cdd:PRK09029 429 YLLPPELK--NGGiKISRQALK 448
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
343-542 |
5.99e-14 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 74.26 E-value: 5.99e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 343 YGQTETTAMVGN-SPGQ--PVKIGSmGRPLPGYVVRITD-ADGHPAKEGEiTLALGDARPAGLMQGYqgeggklsgaegd 418
Cdd:PRK07445 261 YGMTETASQIATlKPDDflAGNNSS-GQVLPHAQITIPAnQTGNITIQAQ-SLALGYYPQILDSQGI------------- 325
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 419 iYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA------IKLAIPKAyvlltad 492
Cdd:PRK07445 326 -FETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPhwgevvTAIYVPKD------- 397
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|
gi 91684220 493 aerSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:PRK07445 398 ---PSISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQI 444
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
196-472 |
1.52e-13 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 73.16 E-value: 1.52e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPK--LVRHSQRSYPVGALSTMFwlGLQPGDVHLNISsPGWAKHAWSC--FFAPWnAGATVFVvnq 271
Cdd:cd17640 88 DDLATIIYTSGTTGNPKgvMLTHANLLHQIRSLSDIV--PPQPGDRFLSIL-PIWHSYERSAeyFIFAC-GCSQAYT--- 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 272 prfDAKSLLATIGRCGVTTLCAPPTVWRM-------------FIQEKLADYKVSLRE----VCGAGEPlnPEVIDQVNAA 334
Cdd:cd17640 161 ---SIRTLKDDLKRVKPHYIVSVPRLWESlysgiqkqvskssPIKQFLFLFFLSGGIfkfgISGGGAL--PPHVDTFFEA 235
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 335 WGATIRDGYGQTETTAMVG-NSPGQPVkIGSMGRPLPGYVVRITDADGH-PAKEGEITLALgdARPAGLMQGYQGEGGKL 412
Cdd:cd17640 236 IGIEVLNGYGLTETSPVVSaRRLKCNV-RGSVGRPLPGTEIKIVDPEGNvVLPPGEKGIVW--VRGPQVMKGYYKNPEAT 312
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 91684220 413 S---GAEGdIYRSGDVAFADDDGYLTFVGRTDD-VFKSSDYRISPFELESILLEHEAVAEAAVV 472
Cdd:cd17640 313 SkvlDSDG-WFNTGDLGWLTCGGELVLTGRAKDtIVLSNGENVEPQPIEEALMRSPFIEQIMVV 375
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
70-541 |
3.21e-13 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 72.25 E-value: 3.21e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRG-DHLLLLLGNVVPLWE-TMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAA 147
Cdd:cd05927 7 SYKEVAERADNIGSALRSLGGKPApASFVGIYSINRPEWIiSELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFCD 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 148 PD----QVAKFADLGGDSLIRIVVGSPqdgwrgydeasqqsdsftpdgptqaDDPMLLYFTSGTTAKPKLVRHSQRSYpV 223
Cdd:cd05927 87 AGvkvySLEEFEKLGKKNKVPPPPPKP-------------------------EDLATICYTSGTTGNPKGVMLTHGNI-V 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 224 GALSTMFWLG-----LQPGDVHlnISSPGWAkHA------WSCFFApwnaGATV-FVvnqpRFDAKSLLATIGRCGVTTL 291
Cdd:cd05927 141 SNVAGVFKILeilnkINPTDVY--ISYLPLA-HIfervveALFLYH----GAKIgFY----SGDIRLLLDDIKALKPTVF 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 292 CAPPTVW-RM------------FIQEKLADYKVSLRE---------------------------------VCGAGePLNP 325
Cdd:cd05927 210 PGVPRVLnRIydkifnkvqakgPLKRKLFNFALNYKLaelrsgvvraspfwdklvfnkikqalggnvrlmLTGSA-PLSP 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 326 EVIDQVNAAWGATIRDGYGQTETTAMV-GNSPGQPVkIGSMGRPLPGYVVRITD--------ADGHPakEGEITLalgda 396
Cdd:cd05927 289 EVLEFLRVALGCPVLEGYGQTECTAGAtLTLPGDTS-VGHVGGPLPCAEVKLVDvpemnydaKDPNP--RGEVCI----- 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 397 RPAGLMQGYQGEGGKLSGA---EGdIYRSGDVAFADDDGYLTFVGRTDDVFKSS--DYrISPFELESILLEHEAVAE--- 468
Cdd:cd05927 361 RGPNVFSGYYKDPEKTAEAldeDG-WLHTGDIGEWLPNGTLKIIDRKKNIFKLSqgEY-VAPEKIENIYARSPFVAQifv 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 469 ----------AAVVPSPDAIKlAIPKAYVLLTADAE---RSRDTALSIFRHM-----QQRLAPFKRIRKLELVTELPK-- 528
Cdd:cd05927 439 ygdslksflvAIVVPDPDVLK-EWAASKGGGTGSFEelcKNPEVKKAILEDLvrlgkENGLKGFEQVKAIHLEPEPFSve 517
|
570
....*....|....*..
gi 91684220 529 ----TISGKIRRVHLRR 541
Cdd:cd05927 518 ngllTPTFKLKRPQLKK 534
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
191-539 |
4.94e-13 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 71.22 E-value: 4.94e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 191 GPTQADDPMLLYFTSGTTAKPKLVRHS----QR---SYpVGALStmfwlglQPGDVHLNISSPgwAKHAW---SCFFAPW 260
Cdd:PRK08308 96 VNYLAEEPSLLQYSSGTTGEPKLIRRSwteiDReieAY-NEALN-------CEQDETPIVACP--VTHSYgliCGVLAAL 165
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 261 NAGATVFVVNQPrfDAKSLLATIGRCGVTTLCAPPTVwrMFIQEKLADYKVSLREVCGAGEPLnPEvidqvnaAWGATIR 340
Cdd:PRK08308 166 TRGSKPVIITNK--NPKFALNILRNTPQHILYAVPLM--LHILGRLLPGTFQFHAVMTSGTPL-PE-------AWFYKLR 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 341 dgygqtETTAMV----GNSPGQPVKI-------GSMGRPLPGYVVRITDADGHPakeGEITLALGDARpaglmqgyqgeg 409
Cdd:PRK08308 234 ------ERTTYMmqqyGCSEAGCVSIcpdmkshLDLGNPLPHVSVSAGSDENAP---EEIVVKMGDKE------------ 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 410 gklsgaegdIYrSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKAYVLl 489
Cdd:PRK08308 293 ---------IF-TKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVI- 361
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 91684220 490 tadAERSRDTAlSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHL 539
Cdd:PRK08308 362 ---SHEEIDPV-QLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
109-536 |
1.80e-12 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 69.54 E-value: 1.80e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 109 TMLAAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQvakfaDLGGDSLIRIVVGSPQDGWRgydeasqQSDSFT 188
Cdd:PRK04813 68 TFLGAVKAGHAYIPVDVSSPAERIEMIIEVAKPSLIIATEEL-----PLEILGIPVITLDELKDIFA-------TGNPYD 135
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 189 PDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYpvgaLSTMFWL----GLQPGDVHLNIsspgwakhawscffAPWN--- 261
Cdd:PRK04813 136 FDHAVKGDDNYYIIFTSGTTGKPKGVQISHDNL----VSFTNWMledfALPEGPQFLNQ--------------APYSfdl 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 262 ----------AGATVFVV-----NQPrfdaKSLLATIGRCGVTTLCAPPTVWRM------FIQEKLADYKVSLreVCGag 320
Cdd:PRK04813 198 svmdlyptlaSGGTLVALpkdmtANF----KQLFETLPQLPINVWVSTPSFADMclldpsFNEEHLPNLTHFL--FCG-- 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 321 EPLNPEVIDQVNAAW-GATIRDGYGQTETT----------AMVGNSPGQPVkigsmGRPLPGYVVRITDADGHPA---KE 386
Cdd:PRK04813 270 EELPHKTAKKLLERFpSATIYNTYGPTEATvavtsieitdEMLDQYKRLPI-----GYAKPDSPLLIIDEEGTKLpdgEQ 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 387 GEITLAlGDArpagLMQGYQGEGGKLSGA----EGD-IYRSGDVAFADDdGYLTFVGRTDDVFKSSDYRIspfELESI-- 459
Cdd:PRK04813 345 GEIVIS-GPS----VSKGYLNNPEKTAEAfftfDGQpAYHTGDAGYLED-GLLFYQGRIDFQIKLNGYRI---ELEEIeq 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 460 -LLEHEAVAEAAVVPSPDAIKLAIPKAYVLLTaDAERSRDTALS--IFRHMQQRLAPFKRIRKLELVTELPKTISGKIRR 536
Cdd:PRK04813 416 nLRQSSYVESAVVVPYNKDHKVQYLIAYVVPK-EEDFEREFELTkaIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDR 494
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
320-542 |
8.23e-12 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 66.99 E-value: 8.23e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 320 GEPLNPEVIDQVNAAwGATIRDGYGQTETTAmvgnspgqpvkiGSM--GRPLPGYVVRITDadghpakeGEItlALGDAR 397
Cdd:PRK07824 160 GGPAPAPVLDAAAAA-GINVVRTYGMSETSG------------GCVydGVPLDGVRVRVED--------GRI--ALGGPT 216
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 398 PAglmQGYQGEGGKLSGAEGDIYRSGDVAfADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDA 477
Cdd:PRK07824 217 LA---KGYRNPVDPDPFAEPGWFRTDDLG-ALDDGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDD 292
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 478 -IKLAIPKAYVlltaDAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLRRL 542
Cdd:PRK07824 293 rLGQRVVAAVV----GDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVRR 354
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
192-550 |
8.25e-11 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 64.73 E-value: 8.25e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 192 PTQADDPMLLYFTSGTTAKPKLVRHSQRSY--PVGALSTMfwLGLQPGDVHLNiSSPGWakHAWSC---FFAPWNAGATV 266
Cdd:PRK08043 361 KQQPEDAALILFTSGSEGHPKGVVHSHKSLlaNVEQIKTI--ADFTPNDRFMS-ALPLF--HSFGLtvgLFTPLLTGAEV 435
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 267 FVVNQP-RFDAKSLLATIGRCgvTTLCAPPTVwrmfiqekLADYK--------VSLREVCGAGEPLNPEVIDQVNAAWGA 337
Cdd:PRK08043 436 FLYPSPlHYRIVPELVYDRNC--TVLFGTSTF--------LGNYArfanpydfARLRYVVAGAEKLQESTKQLWQDKFGL 505
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 338 TIRDGYGQTETTAMVG-NSPgQPVKIGSMGRPLPGYVVRITDADGhPAKEGEITLalgdaRPAGLMQGY-------QGEG 409
Cdd:PRK08043 506 RILEGYGVTECAPVVSiNVP-MAAKPGTVGRILPGMDARLLSVPG-IEQGGRLQL-----KGPNIMNGYlrvekpgVLEV 578
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 410 GKLSGAEGDI----YRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKlaiPKA 485
Cdd:PRK08043 579 PTAENARGEMergwYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKSDASK---GEA 655
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 91684220 486 YVLLTADAERSRDTALSIFR-HMQQRLAPFKRIRKLElvtELPKTISGKIRRVHLRRLEHDDDRAD 550
Cdd:PRK08043 656 LVLFTTDSELTREKLQQYAReHGVPELAVPRDIRYLK---QLPLLGSGKPDFVTLKSMVDEPEQHD 718
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
10-383 |
1.78e-10 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 63.66 E-value: 1.78e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 10 FLLEHRTDYAAAVAGFRWPDpapfnwaLDWF-DAEL--ATNA---DSRDRAALWIVdGITGVELKPSFATMSKRSNQVAN 83
Cdd:PRK03584 58 FGVIGSTPYTVVLAGRRMPG-------ARWFpGARLnyAENLlrhRRDDRPAIIFR-GEDGPRRELSWAELRRQVAALAA 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 84 YLRAQGLKRGDHLLLLLGNVvplWET---MLAAIKLGIVvipaTTLLTPD----ELRDRLDRGAAKAVVAAP-------- 148
Cdd:PRK03584 130 ALRALGVGPGDRVAAYLPNI---PETvvaMLATASLGAI----WSSCSPDfgvqGVLDRFGQIEPKVLIAVDgyryggka 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 149 -DQVAKFADLGG--DSLIRIVV---------GSPQDGWRGYDE--ASQQSDSFTPDgPTQADDPMLLYFTSGTTAKPKLV 214
Cdd:PRK03584 203 fDRRAKVAELRAalPSLEHVVVvpylgpaaaAAALPGALLWEDflAPAEAAELEFE-PVPFDHPLWILYSSGTTGLPKCI 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 215 RHSQRsypvGAL-----STMFWLGLQPGDVHLNISSPGWAKhawscffapWNA-------GATVFVVN----QPRFDAks 278
Cdd:PRK03584 282 VHGHG----GILlehlkELGLHCDLGPGDRFFWYTTCGWMM---------WNWlvsgllvGATLVLYDgspfYPDPNV-- 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 279 LLATIGRCGVTTLCAPPTvwrmFIQ--EKlADYKV-------SLREVCGAGEPLNPEVIDQVNAAWGATIR--DGYGQTE 347
Cdd:PRK03584 347 LWDLAAEEGVTVFGTSAK----YLDacEK-AGLVPgethdlsALRTIGSTGSPLPPEGFDWVYEHVKADVWlaSISGGTD 421
|
410 420 430
....*....|....*....|....*....|....*..
gi 91684220 348 -TTAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGHP 383
Cdd:PRK03584 422 iCSCFVGGNPLLPVYRGEIQCRGLGMAVEAWDEDGRP 458
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
196-540 |
2.27e-10 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 62.83 E-value: 2.27e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDvhlNISSPGWAKHAWSCFFAPWN---AGATVFVvnQP 272
Cdd:cd05937 87 DDPAILIYTSGTTGLPKAAAISWRRTLVTSNLLSHDLNLKNGD---RTYTCMPLYHGTAAFLGACNclmSGGTLAL--SR 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 273 RFDAKSLL-------ATI----GRCGVTTLCAPPTVWrmfiqeklaDYKVSLREVCGAGepLNPEVIDQVNAAWG-ATIR 340
Cdd:cd05937 162 KFSASQFWkdvrdsgATIiqyvGELCRYLLSTPPSPY---------DRDHKVRVAWGNG--LRPDIWERFRERFNvPEIG 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 341 DGYGQTETTAMVGNSPGQPVKIGSMGRPLP--------GYV-VRITDADGHP--------------AKEGEITLALgDAR 397
Cdd:cd05937 231 EFYAATEGVFALTNHNVGDFGAGAIGHHGLirrwkfenQVVlVKMDPETDDPirdpktgfcvrapvGEPGEMLGRV-PFK 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 398 PAGLMQGYQGEGGKLSGA-------EGDIY-RSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEA 469
Cdd:cd05937 310 NREAFQGYLHNEDATESKlvrdvfrKGDIYfRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEA 389
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 91684220 470 AV----VPSPD--AIKLAIpkayVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKIRRVHLR 540
Cdd:cd05937 390 NVygvkVPGHDgrAGCAAI----TLEESSAVPTEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLR 462
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
175-467 |
7.35e-10 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 61.92 E-value: 7.35e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 175 RGYDEASQQSdsftPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWL-----GLQPGDVHLN------- 242
Cdd:PTZ00216 247 KGHSAGSHHP----LNIPENNDDLALIMYTSGTTGDPKGVMHTHGSLTAGILALEDRLndligPPEEDETYCSylplahi 322
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 243 ----ISSPGWAKHAWSCFFAPWNAGAT-------------VFVVNQPR-FDaksllaTIGRcGVTTLCAPP-TVWRMFI- 302
Cdd:PTZ00216 323 mefgVTNIFLARGALIGFGSPRTLTDTfarphgdltefrpVFLIGVPRiFD------TIKK-AVEAKLPPVgSLKRRVFd 395
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 303 ---QEKLA-----------DYKV----------SLREVCGAGEPLNPEVIDQVNAAWGATIRdGYGQTETtamVGNSPGQ 358
Cdd:PTZ00216 396 hayQSRLRalkegkdtpywNEKVfsapravlggRVRAMLSGGGPLSAATQEFVNVVFGMVIQ-GWGLTET---VCCGGIQ 471
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 359 ---PVKIGSMGRPLPGYVVRITDADGH-----PAKEGEITLalgdaRPAGLMQGY--QGEGGKLSGAEGDIYRSGDVAFA 428
Cdd:PTZ00216 472 rtgDLEPNAVGQLLKGVEMKLLDTEEYkhtdtPEPRGEILL-----RGPFLFKGYykQEELTREVLDEDGWFHTGDVGSI 546
|
330 340 350 360
....*....|....*....|....*....|....*....|
gi 91684220 429 DDDGYLTFVGRTDDVFKSSDYRISPFE-LESILLEHEAVA 467
Cdd:PTZ00216 547 AANGTLRIIGRVKALAKNCLGEYIALEaLEALYGQNELVV 586
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
40-542 |
3.73e-09 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 59.24 E-value: 3.73e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 40 FDAELATNADSRDRAalwIVDGITGVELKPSFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIV 119
Cdd:PRK07768 4 FTEKMYANARTSPRG---MVTGEPDAPVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGAS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 120 VipaTTLLTP----------DELRDRLDR-GAAKAVVAAPDQVAKFAdLGGDSLIRIVVGSPQDGwrgydeasqqsDSFT 188
Cdd:PRK07768 81 L---TMLHQPtprtdlavwaEDTLRVIGMiGAKAVVVGEPFLAAAPV-LEEKGIRVLTVADLLAA-----------DPID 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 189 PDgPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPG-DVHLnisspgwakhAW----------SCFF 257
Cdd:PRK07768 146 PV-ETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAEFDVEtDVMV----------SWlplfhdmgmvGFLT 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 258 APWNAGATVFVVNQPRFDAKSLL--ATIGRCGVTTLCAPPTVW-----RMFIQEKLADYKVS-LR-EVCGAgEPLNPEVI 328
Cdd:PRK07768 215 VPMYFGAELVKVTPMDFLRDPLLwaELISKYRGTMTAAPNFAYallarRLRRQAKPGAFDLSsLRfALNGA-EPIDPADV 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 329 DQVNAAWG------ATIRDGYGQTETTAMVGNSP--------------------GQPVKIG------SMGRPLPGYVVRI 376
Cdd:PRK07768 294 EDLLDAGArfglrpEAILPAYGMAEATLAVSFSPcgaglvvdevdadllaalrrAVPATKGntrrlaTLGPPLPGLEVRV 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 377 TDADGH--PAKE-GEITLAlGDArpagLMQGYQGEGGKLSGAEGD-IYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRIS 452
Cdd:PRK07768 374 VDEDGQvlPPRGvGVIELR-GES----VTPGYLTMDGFIPAQDADgWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIY 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 453 PFELEsillehEAVAEAAVVPSPDAIklaipkAYVLltaDAERSRDT-ALSIFRHMQQRLAPFKRIRKL---ELVTE--- 525
Cdd:PRK07768 449 PTDIE------RAAARVEGVRPGNAV------AVRL---DAGHSREGfAVAVESNAFEDPAEVRRIRHQvahEVVAEvgv 513
|
570 580
....*....|....*....|....*....
gi 91684220 526 ------------LPKTISGKIRRVHLRRL 542
Cdd:PRK07768 514 rprnvvvlgpgsIPKTPSGKLRRANAAEL 542
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
365-541 |
5.79e-09 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 58.62 E-value: 5.79e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 365 MGRPLPGYVVRITDADGhPAKEGEITLALGDARPAGLMQGYQGEGgklSGAEGDIYRSGDVAFADDDGyLTFVGRTDDVF 444
Cdd:PRK05851 347 LGNPIPGMEVRISPGDG-AAGVAGREIGEIEIRGASMMSGYLGQA---PIDPDDWFPTGDLGYLVDGG-LVVCGRAKELI 421
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 445 KSSDYRISPFELESILLEHEAVAEAAVVPSPDAIKLAIPKayvLLTADAERSRDTALSiFRHMQQRLAPFKRIRKLELV- 523
Cdd:PRK05851 422 TVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSARPG---LVIAAEFRGPDEAGA-RSEVVQRVASECGVVPSDVVf 497
|
170 180
....*....|....*....|.
gi 91684220 524 ---TELPKTISGKIRRVHLRR 541
Cdd:PRK05851 498 vapGSLPRTSSGKLRRLAVKR 518
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
196-482 |
1.94e-08 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 57.13 E-value: 1.94e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 196 DDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPrFD 275
Cdd:PRK06334 183 EDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSGVPVVFAYNP-LY 261
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 276 AKSLLATIGRCGVTTLCAPPTVWRMFIQ--EKLADYKVSLREVCGAGEPLNPEVIDQVNAAW-GATIRDGYGQTETTAMV 352
Cdd:PRK06334 262 PKKIVEMIDEAKVTFLGSTPVFFDYILKtaKKQESCLPSLRFVVIGGDAFKDSLYQEALKTFpHIQLRQGYGTTECSPVI 341
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 353 G-NSPGQPVKIGSMGRPLPGYVVRITDADGH-PAKEGEITLALgdARPAGLMQGYQGEG---GKLSGAEGDIYRSGDVAF 427
Cdd:PRK06334 342 TiNTVNSPKHESCVGMPIRGMDVLIVSEETKvPVSSGETGLVL--TRGTSLFSGYLGEDfgqGFVELGGETWYVTGDLGY 419
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 428 ADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAEAavvpsPDAIKLAI 482
Cdd:PRK06334 420 VDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEGFGQNAA-----DHAGPLVV 469
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
313-483 |
9.39e-08 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 54.91 E-value: 9.39e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 313 LREVCGAGEPLNPEVIDQVNAAwGATIRDGYGQTETTAMVG-NSPGQPVKiGSMGRPLPGYVVRITDAD--GH----PAK 385
Cdd:cd17639 252 LRYMLSGGAPLSADTQEFLNIV-LCPVIQGYGLTETCAGGTvQDPGDLET-GRVGPPLPCCEIKLVDWEegGYstdkPPP 329
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 386 EGEITLalgdaRPAGLMQGY--QGEGGKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSS--DYrISPFELESILL 461
Cdd:cd17639 330 RGEILI-----RGPNVFKGYykNPEKTKEAFDGDGWFHTGDIGEFHPDGTLKIIDRKKDLVKLQngEY-IALEKLESIYR 403
|
170 180
....*....|....*....|....*
gi 91684220 462 EHEAVAEAAVVPSPDAIK---LAIP 483
Cdd:cd17639 404 SNPLVNNICVYADPDKSYpvaIVVP 428
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
204-538 |
2.03e-07 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 53.61 E-value: 2.03e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 204 TSGTTAKPKLVRHSQRSYPVGALST---MFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVN--QPRFdaks 278
Cdd:COG1541 91 SSGTTGKPTVVGYTRKDLDRWAELFarsLRAAGVRPGDRVQNAFGYGLFTGGLGLHYGAERLGATVIPAGggNTER---- 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 279 LLATIGRCGVTTLCA-PPTVWRM---FIQEKLADYKVSLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVGN 354
Cdd:COG1541 167 QLRLMQDFGPTVLVGtPSYLLYLaevAEEEGIDPRDLSLKKGIFGGEPWSEEMRKEIEERWGIKAYDIYGLTEVGPGVAY 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 355 -SPGQpvkigsmgrplPGYVVR--------ITDADGHPAKEGE-----ITlALG-DARPagLmqgyqgeggklsgaegdI 419
Cdd:COG1541 247 eCEAQ-----------DGLHIWedhflveiIDPETGEPVPEGEegelvVT-TLTkEAMP--L-----------------I 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 420 -YRSGDVAFADDD-----------GYLTfvGRTDDVFKssdYR---ISPFELESILLEHEAVAEA--AVVPSPDAI-KLA 481
Cdd:COG1541 296 rYRTGDLTRLLPEpcpcgrthpriGRIL--GRADDMLI---IRgvnVFPSQIEEVLLRIPEVGPEyqIVVDREGGLdELT 370
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 482 IpkaYVLLTADAERSRDTAlSIFRHMQQRLapfkRIR-KLELVT--ELPKTiSGKIRRVH 538
Cdd:COG1541 371 V---RVELAPGASLEALAE-AIAAALKAVL----GLRaEVELVEpgSLPRS-EGKAKRVI 421
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
70-445 |
6.33e-07 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 52.05 E-value: 6.33e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGI---VVIPATTLLTPD-----ELRDRLDRGAA 141
Cdd:cd05921 27 TYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVpaaPVSPAYSLMSQDlaklkHLFELLKPGLV 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 142 KAVVAAPDQVAkFADLGGDSLIRIVVGSPQDGWRGYDEASQQSDSFTPDGP-----TQADDPMLLYFTSGTTAKPKLVRH 216
Cdd:cd05921 107 FAQDAAPFARA-LAAIFPLGTPLVVSRNAVAGRGAISFAELAATPPTAAVDaafaaVGPDTVAKFLFTSGSTGLPKAVIN 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 217 SQRSYPVGALSTMFWLGLQPGDVHLNISspgWAkhAWSCFFAPwNAGATVFVVNQPRF---DAKSLLATIGrcgvTTL-- 291
Cdd:cd05921 186 TQRMLCANQAMLEQTYPFFGEEPPVLVD---WL--PWNHTFGG-NHNFNLVLYNGGTLyidDGKPMPGGFE----ETLrn 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 292 ---CAP------PTVWRMFIQEKLAD--------YKVSLREVCGAGepLNPEVIDQVNAAWGATIRD------GYGQTET 348
Cdd:cd05921 256 lreISPtvyfnvPAGWEMLVAALEKDealrrrffKRLKLMFYAGAG--LSQDVWDRLQALAVATVGEripmmaGLGATET 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 349 TAMVGNSPGQPVKIGSMGRPLPGYVVRITDADGhpakEGEITLalgdaRPAGLMQGY--QGEGGKLSGAEGDIYRSGDVA 426
Cdd:cd05921 334 APTATFTHWPTERSGLIGLPAPGTELKLVPSGG----KYEVRV-----KGPNVTPGYwrQPELTAQAFDEEGFYCLGDAA 404
|
410 420
....*....|....*....|....
gi 91684220 427 -FADDD----GyLTFVGRTDDVFK 445
Cdd:cd05921 405 kLADPDdpakG-LVFDGRVAEDFK 427
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
36-352 |
6.44e-07 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 52.07 E-value: 6.44e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 36 ALDWFDAELATNADSRDRAA--LWIVDGITGVELKpsfatmsKRSNQVANYLRA-QGLKRGDhLLLLLGNVVPLWETM-L 111
Cdd:cd17632 40 ALGQRATELVTDPATGRTTLrlLPRFETITYAELW-------ERVGAVAAAHDPeQPVRPGD-FVAVLGFTSPDYATVdL 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 112 AAIKLGIVVIPATTLLTPDELRDRLDRGAAKAVVAAPDQ--VAKFADLGGDSLIRIVV--GSPQDG-------------- 173
Cdd:cd17632 112 ALTRLGAVSVPLQAGASAAQLAPILAETEPRLLAVSAEHldLAVEAVLEGGTPPRLVVfdHRPEVDahraalesarerla 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 174 -----WRGYDEASQQSDSFTP---DGPTQADDPM-LLYFTSGTTAKPKLVRHSQRsypvgaLSTMFWLGL-------QPG 237
Cdd:cd17632 192 avgipVTTLTLIAVRGRDLPPaplFRPEPDDDPLaLLIYTSGSTGTPKGAMYTER------LVATFWLKVssiqdirPPA 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 238 DVHLN------ISSPGWakhawscFFAPWNAGATVFVVnqPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADY-- 309
Cdd:cd17632 266 SITLNfmpmshIAGRIS-------LYGTLARGGTAYFA--AASDMSTLFDDLALVRPTELFLVPRVCDMLFQRYQAELdr 336
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 91684220 310 ---------------KVSLRE----------VCGAGePLNPEVIDQVNAAWGATIRDGYGQTETTAMV 352
Cdd:cd17632 337 rsvagadaetlaervKAELRErvlggrllaaVCGSA-PLSAEMKAFMESLLDLDLHDGYGSTEAGAVI 403
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
312-542 |
1.01e-06 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 51.89 E-value: 1.01e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 312 SLREVCGAGEPLNPEVIDQVNAAWGATIRDGYGQTETTAMVG-NSPGQPvKIGSMGRPLPGYVVRITDADGHPakEGEIT 390
Cdd:PRK06814 908 SLRYVFAGAEKVKEETRQTWMEKFGIRILEGYGVTETAPVIAlNTPMHN-KAGTVGRLLPGIEYRLEPVPGID--EGGRL 984
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 391 LALGDarpaGLMQGY-QGEG-GKLSGAEGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESILLEHEAVAE 468
Cdd:PRK06814 985 FVRGP----NVMLGYlRAENpGVLEPPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDAL 1060
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 91684220 469 AAVVPSPDAIKlaiPKAYVLLTADAERSRDTALSIFRHMQQ-RLAPFKRIRKLElvtELPKTISGKIRRVHLRRL 542
Cdd:PRK06814 1061 HAAVSIPDARK---GERIILLTTASDATRAAFLAHAKAAGAsELMVPAEIITID---EIPLLGTGKIDYVAVTKL 1129
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
42-542 |
3.27e-05 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 46.92 E-value: 3.27e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 42 AELATNADSRDRAALwivdGITGV-------ELKP--SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLA 112
Cdd:PRK09192 18 ADFPTLVEALDYAAL----GEAGMnfydrrgQLEEalPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFA 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 113 AIKLGIVVIPattLLTP----------DELRDRLDRGAAKAVVAaPDQVAKFADLGGDSLIRIVVGSPQDgwrgYDEASQ 182
Cdd:PRK09192 94 CQYAGLVPVP---LPLPmgfggresyiAQLRGMLASAQPAAIIT-PDELLPWVNEATHGNPLLHVLSHAW----FKALPE 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 183 QSDSFTPDGPtqaDDPMLLYFTSGTTAKPKLVRHSQRSYPVGALS-TMFWLGLQPGDvhLNISspgwakhaW-------- 253
Cdd:PRK09192 166 ADVALPRPTP---DDIAYLQYSSGSTRFPRGVIITHRALMANLRAiSHDGLKVRPGD--RCVS--------Wlpfyhdmg 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 254 --SCFFAPWNAGATVFVVNQPRFDAKSL--LATIGRCGVTT---------LCApptvwRMFIQEKLADYKVSLREVCGAG 320
Cdd:PRK09192 233 lvGFLLTPVATQLSVDYLPTRDFARRPLqwLDLISRNRGTIsysppfgyeLCA-----RRVNSKDLAELDLSCWRVAGIG 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 321 -EPLNPEVIDQ-VNAAWGATIRD-----GYGQTETTAMVGNSP----------------GQPVKIGSM------------ 365
Cdd:PRK09192 308 aDMIRPDVLHQfAEAFAPAGFDDkafmpSYGLAEATLAVSFSPlgsgivveevdrdrleYQGKAVAPGaetrrvrtfvnc 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 366 GRPLPGYVVRITDADGHPAKEGEITLALgdARPAGLMQGY--QGEGGKLSGAEG--DiyrSGDVAFAdDDGYLTFVGRTD 441
Cdd:PRK09192 388 GKALPGHEIEIRNEAGMPLPERVVGHIC--VRGPSLMSGYfrDEESQDVLAADGwlD---TGDLGYL-LDGYLYITGRAK 461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 442 DVFKSSDYRISPFELEsillehEAVAEAAVVPSPDAIKLAIPK----AYVLL----TADAERSRDtalsifrhMQQRLAP 513
Cdd:PRK09192 462 DLIIINGRNIWPQDIE------WIAEQEPELRSGDAAAFSIAQengeKIVLLvqcrISDEERRGQ--------LIHALAA 527
|
570 580 590
....*....|....*....|....*....|....*.
gi 91684220 514 FKRIR-----KLELVT--ELPKTISGKIRRVHLRRL 542
Cdd:PRK09192 528 LVRSEfgveaAVELVPphSLPRTSSGKLSRAKAKKR 563
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
322-479 |
4.02e-05 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 46.63 E-value: 4.02e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 322 PLNPEVIDQVNAAWGATIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPGYVVRITD-------ADGHPAKEGEITLalg 394
Cdd:PLN02736 387 PLSPDVMEFLRICFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKLVDvpemnytSEDQPYPRGEICV--- 463
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 395 daRPAGLMQGY---QGEGGKLSGAEGDIYrSGDVAFADDDGYLTFVGRTDDVFK--SSDYrISPFELESILLEHEAVAE- 468
Cdd:PLN02736 464 --RGPIIFKGYykdEVQTREVIDEDGWLH-TGDIGLWLPGGRLKIIDRKKNIFKlaQGEY-IAPEKIENVYAKCKFVAQc 539
|
170 180
....*....|....*....|...
gi 91684220 469 ------------AAVVPSPDAIK 479
Cdd:PLN02736 540 fvygdslnsslvAVVVVDPEVLK 562
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
44-540 |
4.53e-05 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 46.23 E-value: 4.53e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 44 LATNADSRDR--AALWIVDGITGveLKPSFATMS---KRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGI 118
Cdd:PLN03052 181 LTPKPSKTDDsiAIIWRDEGSDD--LPVNRMTLSelrSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGC 258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 119 VVIPATTLLTPDELRDRLDRGAAKA-------------------VVAAPDQVAKFADLGGDSlIRIVVGSPQDGWRGY-- 177
Cdd:PLN03052 259 VVVSIADSFAPSEIATRLKISKAKAiftqdvivrggksiplysrVVEAKAPKAIVLPADGKS-VRVKLREGDMSWDDFla 337
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 178 -DEASQQSDSFTP-DGPTQADDPMLlyFTSGTTAKPKLVRHSQRSyPVGALSTMfW--LGLQPGDVHLNISSPGWAKHAW 253
Cdd:PLN03052 338 rANGLRRPDEYKAvEQPVEAFTNIL--FSSGTTGEPKAIPWTQLT-PLRAAADA-WahLDIRKGDIVCWPTNLGWMMGPW 413
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 254 SCFFAPWNaGATVFVVN-QP------RF--DAKsllatigrcgVTTLCAPPTVWRMFIQEK-LADYKVS-LREVCGAGEP 322
Cdd:PLN03052 414 LVYASLLN-GATLALYNgSPlgrgfaKFvqDAK----------VTMLGTVPSIVKTWKNTNcMAGLDWSsIRCFGSTGEA 482
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 323 LNpevIDQV----NAAWGATIRDGYGQTETTAM-VGNSPGQPVKIGSMGRPLPGYVVRITDADGHP-----AKEGEITLA 392
Cdd:PLN03052 483 SS---VDDYlwlmSRAGYKPIIEYCGGTELGGGfVTGSLLQPQAFAAFSTPAMGCKLFILDDSGNPypddaPCTGELALF 559
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 393 ---LGDARPagLMQG------YQG----EGGKLSgaegdiyRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESI 459
Cdd:PLN03052 560 plmFGASST--LLNAdhykvyFKGmpvfNGKILR-------RHGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERV 630
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 460 L-LEHEAVAE-AAV-VPSPDA--IKLAIPKAYVLLTADAERSRDTALSIFRHMQQRLAPFKRIRKLELVTELPKTISGKI 534
Cdd:PLN03052 631 CnAADESVLEtAAIgVPPPGGgpEQLVIAAVLKDPPGSNPDLNELKKIFNSAIQKKLNPLFKVSAVVIVPSFPRTASNKV 710
|
....*..
gi 91684220 535 -RRVhLR 540
Cdd:PLN03052 711 mRRV-LR 716
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
195-541 |
5.67e-05 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 45.94 E-value: 5.67e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 195 ADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLnisspGWA--KHAW---SCFFAPWNAGATVFVV 269
Cdd:cd05908 105 ADELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRIL-----SWMplTHDMgliAFHLAPLIAGMNQYLM 179
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 270 NQPRFDAKSL--LATIGRCGVTTLCAPPTVWRMFIQ----EKLADYKVS-LREVCGAGEPLNPEVIDQVNAAWGA----- 337
Cdd:cd05908 180 PTRLFIRRPIlwLKKASEHKATIVSSPNFGYKYFLKtlkpEKANDWDLSsIRMILNGAEPIDYELCHEFLDHMSKyglkr 259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 338 -TIRDGYGQTETTAMVGNSP-----------------GQPVK-----------IGSMGRPLPGYVVRITDADGHPAKEGE 388
Cdd:cd05908 260 nAILPVYGLAEASVGASLPKaqspfktitlgrrhvthGEPEPevdkkdsecltFVEVGKPIDETDIRICDEDNKILPDGY 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 389 ItlalGDARPAGL--MQGYQG---EGGKLSGAEGDIyRSGDVAFADDdGYLTFVGRTDDVFKSSDYRISPFELESILLEH 463
Cdd:cd05908 340 I----GHIQIRGKnvTPGYYNnpeATAKVFTDDGWL-KTGDLGFIRN-GRLVITGREKDIIFVNGQNVYPHDIERIAEEL 413
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 464 EAVAEAAVV--------PSPDAIKLAIPKAyvlltADAERSRDTALSIFRHMQQRLApfKRIRKLELVTELPKTISGKIR 535
Cdd:cd05908 414 EGVELGRVVacgvnnsnTRNEEIFCFIEHR-----KSEDDFYPLGKKIKKHLNKRGG--WQINEVLPIRRIPKTTSGKVK 486
|
....*.
gi 91684220 536 RVHLRR 541
Cdd:cd05908 487 RYELAQ 492
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
201-492 |
2.68e-04 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 43.89 E-value: 2.68e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 201 LYFTSGTTAKPKLV--RHSQRSYPVGALSTMfwLGLQPGDV-------HLNISSPGwAK--HAWSCFFApwnAGATVFVv 269
Cdd:cd05933 155 LIYTSGTTGMPKGVmlSHDNITWTAKAASQH--MDLRPATVgqesvvsYLPLSHIA-AQilDIWLPIKV---GGQVYFA- 227
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 270 nQPrfDA--KSLLATIGRCGVTTLCAPPTVWRMfIQEK------------------------------------------ 305
Cdd:cd05933 228 -QP--DAlkGTLVKTLREVRPTAFMGVPRVWEK-IQEKmkavgaksgtlkrkiaswakgvgletnlklmggespsplfyr 303
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 306 LADYKV--SLREVCG---------AGEPLNPEVID---QVNAAwgatIRDGYGQTETTAMVGNSPGQPVKIGSMGRPLPG 371
Cdd:cd05933 304 LAKKLVfkKVRKALGldrcqkfftGAAPISRETLEfflSLNIP----IMELYGMSETSGPHTISNPQAYRLLSCGKALPG 379
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 372 YVVRIT--DADGHpakeGEITLalgdaRPAGLMQGYQGEGGKLSGA--EGDIYRSGDVAFADDDGYLTFVGRTDDVFK-S 446
Cdd:cd05933 380 CKTKIHnpDADGI----GEICF-----WGRHVFMGYLNMEDKTEEAidEDGWLHSGDLGKLDEDGFLYITGRIKELIItA 450
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 91684220 447 SDYRISPfelesilleheavaeaavVPSPDAIKLAIPK-AYVLLTAD 492
Cdd:cd05933 451 GGENVPP------------------VPIEDAVKKELPIiSNAMLIGD 479
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
70-219 |
3.50e-04 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 43.50 E-value: 3.50e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGIVVIPAT---TLLTPD--ELRDRLDRGAAKAV 144
Cdd:PRK12582 82 TYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVSpaySLMSHDhaKLKHLFDLVKPRVV 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VAapDQVAKFA------DLGGDSLIriVVGSPQDGWRGY-----------DEASQQSDSFTPDgpTQADdpmlLYFTSGT 207
Cdd:PRK12582 162 FA--QSGAPFAralaalDLLDVTVV--HVTGPGEGIASIafadlaatpptAAVAAAIAAITPD--TVAK----YLFTSGS 231
|
170
....*....|..
gi 91684220 208 TAKPKLVRHSQR 219
Cdd:PRK12582 232 TGMPKAVINTQR 243
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
70-219 |
1.78e-03 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 41.02 E-value: 1.78e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 70 SFATMSKRSNQVANYLRAQGLKRGDHLLLLLGNVVPLWETMLAAIKLGI---VVIPATTLLTPD--ELRDRLDRGAAKAV 144
Cdd:PRK08180 71 TYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALLALAAMYAGVpyaPVSPAYSLVSQDfgKLRHVLELLTPGLV 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 91684220 145 VAapDQVAKFAD----LGGDSLIRIVVGSPQDGWRGYDEASQQSDSFTPD-----GPTQADDPMLLYFTSGTTAKPKLVR 215
Cdd:PRK08180 151 FA--DDGAAFARalaaVVPADVEVVAVRGAVPGRAATPFAALLATPPTAAvdaahAAVGPDTIAKFLFTSGSTGLPKAVI 228
|
....
gi 91684220 216 HSQR 219
Cdd:PRK08180 229 NTHR 232
|
|
| |
