4G36,2D1Q,2D1S,4M46,5KYT


Conserved Protein Domain Family
Firefly_Luc
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cd17642: Firefly_Luc 
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insect luciferase, similar to plant 4-coumarate: CoA ligases
This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Links
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Statistics
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PSSM-Id: 341297
Aligned: 7 rows
Threshold Bit Score: 896.887
Created: 27-Jan-2017
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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AMP bindingacyl-activating
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:AMP binding site [chemical binding site]
Evidence:
  • Structure:2D1Q; Luciola cruciata luciferase binds AMP, contacts at 4A.
    - View structure with Cn3D
  • Citation:PMID 8805533
  • Structure:2D1S; Luciola cruciata luciferase binds 50-O-[N-(dehydroluciferyl)-sulfamoyl adenosine (DLSA), contacts at 4A.
    - View structure with Cn3D
  • Comment:luciferyl adenylate releases AMP
  • Comment:Luciferase produces light in the presence of MgATP, molecular oxygen and luciferin
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 

Feature 1                                                                                     
4G36_A     12 IKKGPAP.[1].YPLED.[2].AGEQLHKAMKRYA.[5].IAFTDA.[3].VNITYAEYFEMSVRLAEAMK.[7].HRIV 84  common eastern firefly
2D1Q_A     10 IVVGPKP.[1].YPIEE.[2].AGTQLRKYMERYA.[4].IAFTNA.[3].VDYSYAEYLEKSXXLGKALQ.[7].GRIA 81  Japanese firefly
2D1S_A     10 IVVGPKP.[1].YPIEE.[2].AGTQLRKYMERYA.[4].IAFTNA.[3].VDYSYAEYLEKSCXLGKALQ.[7].GRIA 81  Japanese firefly
Q27757      6 ILYGPEP.[1].HPLAD.[2].AGEQMFYALSRYA.[5].IALTNA.[3].ENVLYEEFLKLSCRLAESFK.[7].DTIA 78  Pennsylania firefly
NP_651221   9 NIVYGGP.[1].TERQA.[2].SRSLGQYILDKYK.[5].TVLVDA.[3].VEYSASFMHKSIVRLAYILQ.[7].DVVG 81  fruit fly
4M46_A     41 IMHGPPP.[1].YPLED.[2].AGEQLHKAMKRYA.[5].IAFTDA.[3].VNITYSEYFEMACRLAETMK.[7].HHIA 113 Iranian firefly
5KYT_A      7 IKKGPAP.[1].YPLED.[2].AGEQLHKAMKRYA.[5].IAFTDA.[3].VNITYAEYFEMSVRLAEAMK.[7].HRIV 79  common eastern firefly


Feature 1                                                                                    
4G36_A     85 VCSENSLQFFMPVLGALFIGVAVAPANDIY.[2].RELLNSMNISQPTVVFVSK.[2].LQKILNVQK.[6].KIIIMD 158 common eastern firefly
2D1Q_A     82 LCSENCEEFFIPVIAGLFIGVGVAPTNEIY.[2].RELVHSLGISKPTIVFSSK.[2].LDKVITVQK.[6].TIVILD 155 Japanese firefly
2D1S_A     82 LCSENCEEFFIPVIAGLFIGVGVAPTNEIY.[2].RELVHSLGISKPTIVFSSK.[2].LDKVITVQK.[6].TIVILD 155 Japanese firefly
Q27757     79 VCSENGLQFFLPLIASLYLGIIAAPVSDKY.[2].RELIHSLGIVKPRIIFCSK.[2].FQKVLNVKS.[6].TIIILD 152 Pennsylania firefly
NP_651221  82 LSSENSVNFALAMFAGLAVGATVAPLNVTY.[2].REVDHAINLSKPKIIFASK.[2].IDRVAKVAS.[6].GIIALS 155 fruit fly
4M46_A    114 VCSENSLQFFMPVCGALFIGVGVAPTNDIY.[2].RELYNSLSISQPTIVFCSK.[2].LQKILGVQK.[6].KIVILD 187 Iranian firefly
5KYT_A     80 VCSENSLQFFMPVLGALFIGVAVAPANDIY.[2].RELLNSMNISQPTVVFVSK.[2].LQKILNVQK.[6].KIIIMD 153 common eastern firefly


Feature 1                                                                                 
4G36_A    159 .[2].TDYQG.[1].QSMYTFVTSH.[9].FVPESFD.[3].TIALIMNSSGSTGLPKGVALPHRTACVRFSHARD 229 common eastern firefly
2D1Q_A    156 .[2].VDYRG.[1].QCLDTFIKRN.[9].FKTVEVD.[3].QVALIMNSSGSTGLPKGVQLTHENIVTRFSHARD 226 Japanese firefly
2D1S_A    156 .[2].VDYRG.[1].QCLDTFIKRN.[9].FKTVEVD.[3].QVALIMNSSGSTGLPKGVQLTHENIVTRFSHARD 226 Japanese firefly
Q27757    153 .[2].EDLGG.[1].QCLNNFISQN.[9].FKPNSFN.[3].QVALVMFSSGTTGVSKGVMLTHKNIVARFSHCKD 223 Pennsylania firefly
NP_651221 156 .[9].YDLKE.[1].MEDEKFKTQP.[1].FTSPAAN.[3].DVSLIVCSSGTTGLPKGVQLTQMNLLATLDSQIQ 225 fruit fly
4M46_A    188 .[2].EDYMG.[1].QSMYSFIESH.[9].YIPDSFD.[3].ATALIMNSSGSTGLPKGVELTHKNICVRFSHCRD 258 Iranian firefly
5KYT_A    154 .[2].TDYQG.[1].QSMYTFVTSH.[9].FVPESFD.[3].TIALIMNSSGSTGLPKGVALPHRTACVRFSHARD 224 common eastern firefly


Feature 1                     #                                                               
4G36_A    230 .[10].TAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRF.[4].FLRSLQDYKIQSALLVPTLFSFFAKSTL.[4].DL 311 common eastern firefly
2D1Q_A    227 .[10].TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKF.[4].FLKTLQDYKCTSVILVPTLFAILNKSEL.[4].DL 308 Japanese firefly
2D1S_A    227 .[10].TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKF.[4].FLKTLQDYKCTSVILVPTLFAILNKSEL.[4].DL 308 Japanese firefly
Q27757    224 .[10].TAILTVIPFHHGFGMTTTLGYFTCGFRVALMHTF.[4].FLQSLQDYKVESTLLVPTLMAFFPKSAL.[4].DL 305 Pennsylania firefly
NP_651221 226 .[ 8].VTLLTVIPWFHAFGCLTLITTACVGARLVYLPKF.[4].FLSAIEKYRVMMAFMVPPLMVFLAKHPI.[4].DL 305 fruit fly
4M46_A    259 .[10].TAILTVIPFHHGFGMFTTLGYLTCGFRIVLMYRF.[4].FLRSLQDYKIQSALLVPTLFSFFAKSTL.[4].DL 340 Iranian firefly
5KYT_A    225 .[10].TAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRF.[4].FLRSLQDYKIQTALLVPTLFSFFAKSTL.[4].DL 306 common eastern firefly


Feature 1              ###                   #####                                        
4G36_A    312 SNLHEIASGGAPLSKEVGEAVA.[6].GIRQGYGLTETTS.[1].ILITPEG.[5].AVGKVVP.[1].FEAKVVD 380 common eastern firefly
2D1Q_A    309 SNLVEIASGGAPLSKEVGEAVA.[6].GVRQGYGLTETTS.[1].IIITPEG.[5].ASGKVVP.[1].FKAKVID 377 Japanese firefly
2D1S_A    309 SNLVEIASGGAPLSKEVGEAVA.[6].GVRQGYGLTETTS.[1].IIITPEG.[5].ASGKVVP.[1].FKAKVID 377 Japanese firefly
Q27757    306 SHLKEIASGGAPLSKEIGEMVK.[6].FVRQGYGLTETTS.[1].VLITPDT.[5].STGKIVP.[1].HAVKVVD 374 Pennsylania firefly
NP_651221 306 SSLMVLLCGAAPLSRETEDQIK.[6].FIRQGYGLSESTL.[1].VLVQNDE.[5].SVGVLKV.[1].IYAKVID 374 fruit fly
4M46_A    341 SNLHEIASGGAPLAKEVGEAVA.[6].GIRQGYGLTETTS.[1].IIITPEG.[5].ACGKVVP.[1].FSAKIVD 409 Iranian firefly
5KYT_A    307 SNLHEIASGGAPLSKEVGEAVA.[6].GIRQGYGLTETTS.[1].ILITPEG.[5].AVGKVVP.[1].FEAKVVD 375 common eastern firefly


Feature 1                                           #             #  # # # #    #             
4G36_A    381 .[11].RGELCVRGPMIMSGYVNNPEA.[8].GWLHSGDIAYW.[3].EHFFIVDRLKSLIKYKGYQVAPAELESILLQH 466 common eastern firefly
2D1Q_A    378 .[11].RGEVCVKGPMLMKGYVNNPEA.[8].GWLHTGDIGYY.[3].KHFFIVDRLKSLIKYKGYQVPPAELESVLLQH 463 Japanese firefly
2D1S_A    378 .[11].RGEVCVKGPMLMKGYVNNPEA.[8].GWLHTGDIGYY.[3].KHFFIVDRLKSLIKYKGYQVPPAELESVLLQH 463 Japanese firefly
Q27757    375 .[11].TGELYFKGDMIMKSYYNNEEA.[8].GWLRSGDIAYY.[3].GHFYIVDRLKSLIKYKGYQVAPAEIEGILLQH 460 Pennsylania firefly
NP_651221 375 .[11].RGELCFKGDGIMKGYIGDTKS.[7].GWLHTGDIGYY.[3].FEFFIVDRIKELIKYKGYQVPPAEIEALLLTN 459 fruit fly
4M46_A    410 .[11].RGELCVKGPMIMKGYVNNPEA.[8].GWLHSGDIAYY.[3].GHFFIVDRLKSLIKYKGYQVPPAELESILLQH 495 Iranian firefly
5KYT_A    376 .[11].RGELCVRGPMIMSGYVNNPEA.[8].GWLHSGDIAYW.[3].EHFFIVDRLKSLIKYKGYQVAPAELESILLQH 461 common eastern firefly


Feature 1                                                                                     
4G36_A    467 P.[2].FDAGVAGLP.[5].ELPAAVVVL.[6].TEKEIVDYVASQV.[2].AKKLRGGVVFVDEVPKGLTGKLD.[1]. 537 common eastern firefly
2D1Q_A    464 P.[2].FDAGVAGVP.[5].ELPGAVVVL.[6].TEKEVMDYVASQV.[2].AKRLRGGVRFVDEVPKGLTGKID.[1]. 534 Japanese firefly
2D1S_A    464 P.[2].FDAGVAGVP.[5].ELPGAVVVL.[6].TEKEVMDYVASQV.[2].AKRLRGGVRFVDEVPKGLTGKID.[1]. 534 Japanese firefly
Q27757    461 P.[2].VDAGVTGIP.[5].ELPAAGVVV.[6].NEQIVQNFVSSQV.[2].AKWLRGGVKFLDEIPKGSTGKID.[1]. 531 Pennsylania firefly
NP_651221 460 D.[2].KDAAVIGKP.[5].ELPLAFVVK.[6].TENEVIQFVNDNA.[2].AKRLRGGVIFVDEIPKNPSGKIL.[1]. 530 fruit fly
4M46_A    496 P.[2].FDAGVAGIP.[5].ELPAAVVVL.[6].TEQEVMDYVAGQV.[2].SKRLRGGVKFVDEVPKGLTGKID.[1]. 566 Iranian firefly
5KYT_A    462 P.[2].FDAGVAGLP.[5].ELPAAVVVL.[6].TEKEIVDYVASQV.[2].AKKLRGGVVFVDEVPKGLTGKLD.[1]. 532 common eastern firefly


Feature 1           
4G36_A    538 RKIREI 543 common eastern firefly
2D1Q_A    535 RAIREI 540 Japanese firefly
2D1S_A    535 RAIREI 540 Japanese firefly
Q27757    532 KVLRQM 537 Pennsylania firefly
NP_651221 531 RILREM 536 fruit fly
4M46_A    567 RKIREI 572 Iranian firefly
5KYT_A    533 RKIREI 538 common eastern firefly

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