3NI2,3A9U,3A9V,5BSM


Conserved Protein Domain Family
4CL
?
cd05904: 4CL 
Click on image for an interactive view with Cn3D
4-Coumarate-CoA Ligase (4CL)
4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Links
?
Statistics
?
PSSM-Id: 341230
Aligned: 26 rows
Threshold Bit Score: 627.725
Created: 27-Sep-2011
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3NI2; Populus tomentosa 4-coumarate:CoA ligase binds to intermediate anolog adenosine 59-(3-(4-hydroxyphenyl)propyl)phosphate (APP), contacts at 4A.
  • Comment:also based on the CoA binding site of a related family member with structure
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
3NI2_A     18 IPKNLPLHsyvlenl---snhsskpCLINGa-nGDVYTYADVELTARRVASGLNKIGiq-qgDVIMLFLPSSPEFVLAFL 92  Populus tomentosa
3A9U_A     18 IPKNLPLHsyvlenl---snhsskpCLINGa-nGDVYTYADVELTARRVASGLNKIGiq-qgDVIMLFLPSSPEFVLAFL 92  Populus tomentosa
3A9V_A     18 IPKNLPLHsyvlenl---snhsskpCLINGa-nGDVYTYADVELTARRVASGLNKIGiq-qgDVIMLFLPSSPEFVLAFL 92  Populus tomentosa
5BSM_A     21 IPNHLPLHsycfeni---sefssrpCLINGa-nKQIYTYADVELNSRKVAAGLHKQGiq-pkDTIMILLPNSPEFVFAFI 95  common tobacco
P41636     21 ISDHLPLHsycferv---aefadrpCLIDGa-tDRTYCFSEVELISRKVAAGLAKLGlq-qgQVVMLLLPNCIEFAFVFM 95  loblolly pine
NP_628552  10 PPVDLPIHdavlgga---aafgstpALIDGt-dGTTLTYEQVDRFHRRVAAALAETGvr-kgDVLALHSPNTVAFPLAFY 84  Streptomyces coeli...
Q9LQ12     22 IPDKLTLPefvlqgv---eeytenvAFVEAv-tGKAVTYGDVVRDTKRLAKALTSLGlr-kgQVMVVVLPNVAEYGIIAL 96  thale cress
EDQ60168   22 IPNHMPLAdycleks---sqwpdkvCLIDGv-tGREHRYGEIELSSRRVAAGLDKIGvk-qgDVIALLLPNCAEFVLVFL 96  Physcomitrella pat...
Q0DV32     33 SLTDLLLRradac--------psavALADAaagGRALTFAELRSAVLSTAVALSSRAgvrpgDAVLLLAPNCVLYPVCFF 104 Japanese rice
YP_886703   9 DVTIPPVSvhdflfaglddadagrvALVDSr-tGAETTYGELIGRIEEFAGALAARGig-vgDVVALLSPNSSAFAVAFH 86  Mycobacterium smeg...

Feature 1                                                                                     
3NI2_A     93 GASHRGAIITAANPFStpAELAKHAKASRAKLLITQAc------yYEKVKDFAResdvKVMCVDsapdgclhfseltq-- 164 Populus tomentosa
3A9U_A     93 GASHRGAIITAANPFStpAELAKHAKASRAKLLITQAc------yYEKVKDFAResdvKVMCVDsapdgclhfseltq-- 164 Populus tomentosa
3A9V_A     93 GASHRGAIITAANPFStpAELAKHAKASRAKLLITQAc------yYEKVKDFAResdvKVMCVDsapdgclhfseltq-- 164 Populus tomentosa
5BSM_A     96 GASYLGAISTMANPLFtpAEVVKQAKASSAKIIVTQAc------hVNKVKDYAFendvKIICIDsapegclhfsvltq-- 167 common tobacco
P41636     96 GASVRGAIVTTANPFYkpGEIAKQAKAAGARIIVTLAa------yVEKLADLQShd-vLVITIDdapkegcqhisvlte- 167 loblolly pine
NP_628552  85 AATRAGASVTTVHPLAtaEEFAKQLKDSAARWIVTVSpllstarrAAELAGGVQ----EILVCDsapghrslvdmlas-- 158 Streptomyces coeli...
Q9LQ12     97 GIMSAGGVFSGANPTAlvSEIKKQVEASGARGIITDAt------nYEKVKSLGL----PVIVLGeekiegavnwkdllea 166 thale cress
EDQ60168   97 GAAKRGAVVTTANPFYtaAELEKQIEASGAGIVITQSs------yIEKLAGLNV----QIITVDqhvancmhisvlln-- 164 Physcomitrella pat...
Q0DV32    105 AVTALGAVGTTVNPDYtpREIAKQVSDARAKLVITISa------lVPKIAGLRL----PVILLDddanaaaaslppdatv 174 Japanese rice
YP_886703  87 GILRAGATATTVNALFtaRDIAKQLTDSKAKLLLTVNa------lAPQSREAAA----AVGLTDdavvlldgeslpt--- 153 Mycobacterium smeg...

Feature 1                                                                               #    #
3NI2_A    165 ---------adeneapqvdispdDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDgdnpnlyfhseDVILCVLPMFHI 235 Populus tomentosa
3A9U_A    165 ---------adeneapqvdispdDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDgdnpnlyfhseDVILCVLPMFHI 235 Populus tomentosa
3A9V_A    165 ---------adeneapqvdispdDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDgdnpnlyfhseDVILCVLPMFHI 235 Populus tomentosa
5BSM_A    168 ---------anehdipeveiqpdDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDgenpnlyihseDVMLCVLPLFHI 238 common tobacco
P41636    168 ---------adetqcpavkihpdDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDgenpnlyfhsdDVILCVLPLFHI 238 loblolly pine
NP_628552 159 ----------tapepsvaidpaeDVAALPYSSGTTGTPKGVMLTHRQIATNLAQLEPsmps----apgDRVLAVLPFFHI 224 Streptomyces coeli...
Q9LQ12    167 --------gdkcgdtdneeilqtDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFgvrse--migqIVTLGLIPFFHI 236 thale cress
EDQ60168  165 ---------acedecpqvrihpdDLVCLPYSSGTTGLPKGVMLTHKSLVSSVSQQVDgeapnfnitveDTLMCVLPMFHI 235 Physcomitrella pat...
Q0DV32    175 tlytnlvagvkeadyrrppikqsDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSdqder--regpNVFLCFLPMFHI 252 Japanese rice
YP_886703 154 ----------ghaapqvtfdpatHLAVLPYSSGTTANPKGVMLTHRNLVANVAQIRPlqgm----rrdDRLLAVLPFFHI 219 Mycobacterium smeg...

Feature 1     #   #                                       ##  #                    # ####     
3NI2_A    236 YALNSIMLCGLrVGAPILIMPKFeigsLLGLIEKYKVSIAPVVPPVMMSIAKSPDld--khDLSSLRMIKSGGAPLGKEL 313 Populus tomentosa
3A9U_A    236 YALNSIMLCGLrVGAPILIMPKFeigsLLGLIEKYKVSIAPVVPPVMMSIAKSPDld--khDLSSLRMIKSGGAPLGKEL 313 Populus tomentosa
3A9V_A    236 YALNSIMLCGLrVGAPILIMPKFeigsLLGLIEKYKVSIAPVVPPVMMSIAKSPDld--khDLSSLRMIKSGGAPLGKEL 313 Populus tomentosa
5BSM_A    239 YSLNSVLLCGLrVGAAILIMQKFdivsFLELIQRYKVTIGPFVPPIVLAIAKSPMvd--dyDLSSVRTVMSGAAPLGKEL 316 common tobacco
P41636    239 YSLNSVLLCALrAGAATLIMQKFnlttCLELIQKYKVTVAPIVPPIVLDITKSPIvs--qyDVSSVRIIMSGAAPLGKEL 316 loblolly pine
NP_628552 225 YGLTALMNAPLrLGATVVVLPRFdleqFLAAIQNHRITSLYVAPPIVLALAKHPLva--dyDLSSLRYIVSAAAPLDARL 302 Streptomyces coeli...
Q9LQ12    237 YGIVGICCATMkNKGKVVAMSRYdlriFLNALIAHEVSFAPIVPPIILNLVKNPIvdefdlSKLKLQSVMTAAAPLAPEL 316 thale cress
EDQ60168  236 YSLNSILLCGLrVGATLVIMPKFelpkLLDLIQRHKVTMGPFVPPIVLAIAKNPIve--nyDLSSMRMVMSGAAPLGREL 313 Physcomitrella pat...
Q0DV32    253 FGLSVITYAQLhRGNAIIAMSRFdinsLMEAVQRHRVTHLFCVPPVIIALAKHGKag--kyDLSSLKFIGSGAAPLGKDV 330 Japanese rice
YP_886703 220 YGMTVLLNAALhARAQLVVMPSFdlaeFLGNIAERRCTYAYIAPPVAVALAKHPMvd--syDLSALEGIMSGAASLDAEL 297 Mycobacterium smeg...

Feature 1                   ######   ##                                                       
3NI2_A    314 EDTVRakfpqaRLGQGYGMTEAGPVLAMCLAFakepf---dikpgACGTVVRnAEMKIVDpetga-----slprnQPGEI 385 Populus tomentosa
3A9U_A    314 EDTVRakfpqaRLGQGYGMTEAGPVLAMCLAFakepf---dikpgACGTVVRnAEMKIVDpetga-----slprnQPGEI 385 Populus tomentosa
3A9V_A    314 EDTVRakfpqaRLGQGYGMTEAGPVLAMCLAFakepf---dikpgACGTVVRnAEMKIVDpetga-----slprnQPGEI 385 Populus tomentosa
5BSM_A    317 EDTVRakfpnaKLGQGYGMTEAGPVLAMCLAFakepf---eiksgACGTVVRnAEMKIVDpktgn-----slprnQSGEI 388 common tobacco
P41636    317 EDALRerfpkaIFGQGYGMTEAGPVLAMNLAFaknpf---pvksgSCGTVVRnAQIKILDtetge-----slphnQAGEI 388 loblolly pine
NP_628552 303 AAACSqrlglpPVGQAYGMTELSPGTHVVPLDamad-----appgTVGRLIAgTEMRIVSltdpgt----dlpagESGEI 373 Streptomyces coeli...
Q9LQ12    317 LTAFEakfpnvQVQEAYGLTEHSCITLTHGDPekgqg---iakrnSVGFILPnLEVKFIDpdtgr-----slpknTSGEL 388 thale cress
EDQ60168  314 EDAFRarlpnaVLGQGYGMTEAGPVLAMCLAFaktpf---svkpgSCGTVVRnAEVKIVDtetgm-----slpynQPGEI 385 Physcomitrella pat...
Q0DV32    331 MEVVAkkfpdsEIVQGYGMTETCGIISLEYPEkgqa-----refgSTGTLVSgVEAKIVDiktlk-----hlppnQVGEI 400 Japanese rice
YP_886703 298 GLAVArrl-dcRVVQGYGMSELSPVSHVTPKDgglstvgtvapldSCGWTVPnSESKIVDpetgaeievpaeglsETGEL 376 Mycobacterium smeg...

Feature 1                                    #           #  # #   # ####                      
3NI2_A    386 CIRGDQIMKGYLNDPEATsrtidkEGWLHTGDIGYIDdDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPeiSDAAVV 465 Populus tomentosa
3A9U_A    386 CIRGDQIMKGYLNDPEATsrtidkEGWLHTGDIGYIDdDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPeiSDAAVV 465 Populus tomentosa
3A9V_A    386 CIRGDQIMKGYLNDPEATsrtidkEGWLHTGDIGYIDdDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPeiSDAAVV 465 Populus tomentosa
5BSM_A    389 CIRGDQIMKGYLNDPEATartidkEGWLYTGDIGYIDdDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPniSDAAVV 468 common tobacco
P41636    389 CIRGPEIMKGYINDPESTaatideEGWLHTGDVEYIDdDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPsiADAAVV 468 loblolly pine
NP_628552 374 LIRGPQIMKGYLGRPDATaamideEGWLHTGDVGHVDaDGWLFVVDRVKELIKYKGFQVAPAELEAHLLTHPgvADAAVV 453 Streptomyces coeli...
Q9LQ12    389 CVRSQCVMQGYFMNKEETdktideQGWLHTGDIGYIDdDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPsvEDVAVV 468 thale cress
EDQ60168  386 CIRGPQIMKGYLKNPEATantidkDGFLHTGDVAFIDeDEEMFIVDRVKEIIKFKGFQVPPAELEALLLSHKeiQDAAVV 465 Physcomitrella pat...
Q0DV32    401 CVRGPNVMQGYFNNVQATeft-ikQGWLHTGDLGYFDgGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPeiLDAVVI 479 Japanese rice
YP_886703 377 WFKGPNVMAGYLNNERATretiddDGFLHTGDLARVDaTGCVYIVDRLKELIKYKGYQVPPAELEALLLTHPgiADAAVI 456 Mycobacterium smeg...

Feature 1                                       #     #                         
3NI2_A    466 GLKDed-agEVPVAFVVKsek-sqaTEDEIKQYISKQViFYKRIKRVFFIEAIPKAPSGKILRKNL 529 Populus tomentosa
3A9U_A    466 GLKDed-agEVPVAFVVKsek-sqaTEDEIKQYISKQViFYKRIKRVFFIEAIPKAPSGKILRKNL 529 Populus tomentosa
3A9V_A    466 GLKDed-agEVPVAFVVKsek-sqaTEDEIKQYISKQViFYKRIKRVFFIEAIPKAPSGKILRKNL 529 Populus tomentosa
5BSM_A    469 PMKDeq-agEVPVAFVVRsng-stiTEDEVKDFISKQViFYKRIKRVFFVDAIPKSPSGKILRKDL 532 common tobacco
P41636    469 PQKHee-agEVPVAFVVKss---eiSEQEIKEFVAKQViFYKKIHRVYFVDAIPKSPSGKILRKDL 530 loblolly pine
NP_628552 454 GAYDdd-gnEVPHAFVVRqpaapglAESEIMMYVAERVaPYKRVRRVTFVDAVPRAASGKILRRQL 518 Streptomyces coelicolor
Q9LQ12    469 PLPDee-agEIPAACVVInpk-ateKEEDILNFVAANVaHYKKVRAVHFVDSIPKSLSGKIMRRLL 532 thale cress
EDQ60168  466 SRKDdv-agEVPVAFVVRats-stiTEDEVKDYIAKQVvFYKKIHNVYFVDSVPKSPSGKILRKDL 529 Physcomitrella patens subsp. patens
Q0DV32    480 PFPDak-agEVPIAYVVRspd-sslTEVDVQKFIEKQVaYYKRLKRVTFVGSVPKSASGKILRRQL 543 Japanese rice
YP_886703 457 GVQDpesgeEVPKAFVVRqpg-idlGADEVMAFVAGQVaPYKKVRQVEFIEAVPKSAAGKILRKEL 521 Mycobacterium smegmatis

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap