2R48


Conserved Protein Domain Family
PTS_IIB_fructose

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cd05569: PTS_IIB_fructose 
Click on image for an interactive view with Cn3D
PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII (also referred to as FruAB) is a fructose-specific permease made up of two proteins (FruA and FruB) each containing 3 domains. The FruA protein contains two tandem nonidentical IIB domains and a C-terminal IIC transmembrane domain. Both IIB domains of FruA are included in this alignment. The FruB protein (also referred to as diphosphoryl transfer protein) contains a IIA domain, a domain of unknown function, and an Hpr-like domain called FPr (fructose-inducible HPr). This familiy also includes the IIB domains of several fructose-like PTS permeases including the Frv permease encoded by the frvABXR operon, the Frw permease encoded by the frwACBD operon, the Frx permease encoded by the hrsA gene, and the Fry permease encoded by the fryABC (ypdDGH) operon. FruAB takes up exogenous fructose, releasing the 1-phosphate ester in to the cytoplasm in preparation for metabolism primarily via glycolysis. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include fructose, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and a sensory system with similarity to the bacterial bgl system.
Statistics
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PSSM-Id: 99911
View PSSM: cd05569
Aligned: 94 rows
Threshold Bit Score: 69.8235
Threshold Setting Gi: 120612886
Created: 15-May-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit,(not included in this IIB subgroup) in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             # ##  ##                                                                  
2R48_A        5 LLAITSCPNGIAHTYXAAENLQKAADRLGVSIKVETQGGIgvENKLTEEEIREADAIIIAADRSVnk--dRFIGKKLLSV 82  Bacillus subtili...
ZP_02009838  68 LLAILGADGRTTRAMLAGAALRHAARESGYKLDVDVRGPDt-ASVVDTAALATAKAVVWAGLSPDaa--aRDANPRTLEV 144 Ralstonia picket...
P23387        4 IVAVTAGAKGVAHTHLAAEALSATAQALGHQIRVERHSAEgvEAPLQGAEIAAADVVLIAADLRIed--vRFVTKPVYRT 81  Rhodobacter caps...
ZP_00415608 176 LLIVTACPSGMVTSVLCARLLEAAALRLGWTTRVEVHDPRaiGSPLAEEDIAAADLVVVVKTGELal--dRFAGKRLLQS 253 Azotobacter vine...
YP_856869     3 AILVTACPAGIATSFLAAKRIEQQAKLAGWELTLDVGSSVqpRQVPSKEQIAAADLVLVVASAPVdl--aAYDGKRVYQG 80  Aeromonas hydrop...
ZP_01894621   3 LIIVTACPQGVATSFLAARALERASQQKGWEARTDIRSPQqpLSSLPVDVIASADLVVAATGTPTdl--nAYAGKRLYQI 80  Marinobacter alg...
YP_439105     4 LIGLVAAPARTAAPTFAAHALGRAAQARGIALALETRSALgvQTPLSADQIGEASAVLIAADDPAsideaPFAGKTIHRV 83  Burkholderia tha...
ABA72538      3 LAIVTSCPNGMVTSVLCARLLDAAAQRQGWSTSVEVVDAAhpERELSAATIEAAEWVLLVATGPVdm--tRFIGKKVFRI 80  Pseudomonas fluo...
YP_427057     4 FIAVVGGGERSTQALLVAEALRRAASGAGHRIDIEVRSDQgvVNALSDDAIVRARAVILIGTGDLdv--gRFPGLPKLET 81  Rhodospirillum r...
EDO73728      4 LLAVVGGGDLSTHAVLAAEALRKAAGRRNTPITLELRGKGasGNPLSDAAIAEARAVLLVGEGDLge--aRFGALHRARA 81  Methylobacterium...
Feature 1                         
2R48_A       83 gvqdgIRKPEELIQKALN 100 Bacillus subtilis subsp. subtilis
ZP_02009838 145 tldevLADARAVLARVMH 162 Ralstonia pickettii 12D
P23387       82 staraVTQTAAVLAEALA 99  Rhodobacter capsulatus
ZP_00415608 254 tpadaLQDPQRFLEEAAA 271 Azotobacter vinelandii AvOP
YP_856869    81 smdlaLQDPAALLEAAAS 98  Aeromonas hydrophila subsp. hydrophila ATCC 7966
ZP_01894621  81 pvtaaLPDAAAVLDQAEK 98  Marinobacter algicola DG893
YP_439105    84 sideaIRDADAVLAHASG 101 Burkholderia thailandensis E264
ABA72538     81 apaqaLQDVEDVLRRGAV 98  Pseudomonas fluorescens PfO-1
YP_427057    82 tieavLADVGAVLGRAEG 99  Rhodospirillum rubrum ATCC 11170
EDO73728     82 aiedvLTDVNAVFDRLGA 99  Methylobacterium populi BJ001

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