Conserved Protein Domain Family
DPM_DPG-synthase_like

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cd04179: DPM_DPG-synthase_like 
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily.
DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.
Statistics
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PSSM-Id: 133022
View PSSM: cd04179
Aligned: 419 rows
Threshold Bit Score: 87.6289
Threshold Setting Gi: 91773953
Created: 23-Sep-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Ligand bindingDXD motifPutative
Feature 1:Ligand binding site
Evidence:
  • Comment:Predicted ligand binding site based on similar proteins.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           # #                                                                          
O60762        29 VLLPTYNERENLPLIVWLLVksfse--------sginyEIIIIDDGSpDGTRDVAEqlekiygsdr--illrprekkLGL 98  human
NP_634245      9 IIIPVHNEEENILELYKSLYnilsl--------vgktyEIIYVDDGStDDSFEKIKsiedarvk------vvrfqrnYGK 74  Methanosarcina ...
NP_661221      6 IIVPLYNERESLPEFCESLFaalksselkrcfgdefsfEIIMVDDGStDGSDKVIGelmtdrpel----rlisfrrnYGK 81  Chlorobium tepi...
AAQ65526       6 VVIPLLNEAESIPELFAWIRrvmne--------hgysyEVIFVDDGStDGSWSVIErlqaehpev----kgikfrrnYGK 73  Porphyromonas g...
YP_721128     10 FVIPVYNEEVTIKPLFERILdvmnlg-------kidnyEIIFVDDGSsDRSWIEINklikkhprq---vkgirlrrnFGK 79  Trichodesmium e...
NP_107847      6 ILVPVKDEQDTVQALIGQIEaafeqi------dsaslkEVIFVDDGSrDNTWKEILgatrtyphv----kgvrlrrnFGK 75  Mesorhizobium l...
NP_867173     72 VVIPAMNEEASLSELHGRILevcae--------qnvrvQIVFVDDGStDKTWQKMVslcgtasdqshstsalrlrrnFGK 143 Rhodopirellula ...
YP_001103659  13 LLAPAKNEAESLPLLFAEIRtames--------qhrtwELLVVDDGStDESWRVIAgqaaadprf----rglrlrrnFGK 80  Saccharopolyspo...
ZP_00144074    6 VIAPIYNEKENIGLLVEKIKttlkd--------rftsyEIILVDDGStDGSSELIEkiastdphi----kdyhftknNGQ 73  Fusobacterium n...
YP_680346     13 VVVPVYNEEKNIAELCSRLHqvlss--------lphrfEIILVNDGStDDSADIISemclvfsel----kgidlagnYGQ 80  Cytophaga hutch...
Feature 1                             #                                                          
O60762        99 GTAYIHGMKHAtgnyIIIMDADLSHHPKFIpefirkqkegnfDIVSGTRykgnggvygwdlkRKIISRGANfltqillrp 178 human
NP_634245     75 AAALSCGFKKSkgdiVITMDGDLQDDPKEIprfie--eldkyDMVSGWKskry-----dpisKTLPSRFFNwltrfitgv 147 Methanosarcina ...
NP_661221     82 TEALSAGFRAAsgevVVTIDADMQDDPREIaglvlk-inegfDLVSGWKrern-----dpmsKTVPSKLFNlttrlfsgi 155 Chlorobium tepi...
AAQ65526      74 SAGLQCGFARTqgqvVITMDADLQDSPDEIpelyrmvteggyDLVSGWKrkryd----plfsKNLPSKLFNatarklsgi 149 Porphyromonas g...
YP_721128     80 SSALSAGFKKTrgniIFTLDADLQDDPAEIpkflek-lesgyDLVSGWRkhrn-----dplsKTLPSKLFNgvtsvltgv 153 Trichodesmium e...
NP_107847     76 AAALQAGIANAsgnvIVTMDGDLQDDPKEIsrflde-iragsDVVSGWKqvrh-----dplgKTLPSKLFNrvtakvtgv 149 Mesorhizobium l...
NP_867173    144 AAALSAGFSVArgsiVFTMDADLQDDPTEIprflek-iqgglDVVSGWKqtrh-----dpwhKVLPSRVFNalvssltgv 217 Rhodopirellula ...
YP_001103659  81 AAALAAGVAEVrgalLVTIDADLQDDPAEIprllde-lddgaDLVSGHKaqrk-----dplgKRLPSKFFNwmtglitgl 154 Saccharopolyspo...
ZP_00144074   74 TAALSAGFKYCtgdiVVTMDSDLQTDPEDIylmlp--yldkyDMVNGKRttre-----dgfkRKISSLIGNgvrnfitkd 146 Fusobacterium n...
YP_680346     81 TIALRAGFELAkgdvIIAMDGDLQHDPLDIirfmkk-ieegyDLVGGSKekrp-----dswlKSKLSAMAHklirklaqv 154 Cytophaga hutch...
Feature 1                                               
O60762       179 gaSDLTGSFRLYRKEVlekliekcvsKGYVFQMEMIVRA 217 human
NP_634245    148 kiNDFNCGYKAYHNYVvkninlygefHRYIPALAYWRGY 186 Methanosarcina mazei Go1
NP_661221    156 plHDFNCGLKAYSRELvasldlhgemHRYIPVLAKWKGF 194 Chlorobium tepidum TLS
AAQ65526     150 klHDFNCGLKAYRHEVvenielyndmHRYIPYLAKSAGF 188 Porphyromonas gingivalis W83
YP_721128    154 klHDFNCGLKAYKKEVldcikvygelHRYIPVLAHSLGF 192 Trichodesmium erythraeum IMS101
NP_107847    150 glHDFNCGFKAYRSEVfdnirlygelHRFVPALAHATGF 188 Mesorhizobium loti MAFF303099
NP_867173    218 rlHDHNCGFKAYRREVlaevdlygerHRFIPVLASARGF 256 Rhodopirellula baltica SH 1
YP_001103659 155 rlSDHNCGLKAARTEVyrrvplygelHRYIPALAHQLGY 193 Saccharopolyspora erythraea NRRL 2338
ZP_00144074  147 niKDTGCPLKLFKKEVvksfylfegmHRFLPTLAKINGF 185 Fusobacterium nucleatum subsp. vincentii ATCC 49256
YP_680346    155 dmSYFGATYKAYRAYLlkqsnllgdnHRFLGAIVARKGI 193 Cytophaga hutchinsonii ATCC 33406

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