Pdlim5 PDZ and LIM domain 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pdlim5provided by RGD
Official Full Name: PDZ and LIM domain 5provided by RGD
Primary source: RGD:621076
See related: EnsemblRapid:ENSRNOG00000016419 AllianceGenome:RGD:621076
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Enh; Enh1
Summary: Enables cytoskeletal protein binding activity; protein N-terminus binding activity; and protein kinase C binding activity. Involved in cell growth involved in cardiac muscle cell development; regulation of dendritic spine morphogenesis; and regulation of synapse assembly. Located in cytosol; membrane; and postsynaptic density. Orthologous to human PDLIM5 (PDZ and LIM domain 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Heart (RPKM 667.5), Muscle (RPKM 417.2) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q44

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (233625478..233793582, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (230952251..231120329, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (247815214..247989198, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results suggest that, in healthy conditions, RBM20 and RBM24 cooperate to promote the expression of short ENH isoforms.
Title: RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.
ENH1 plays an essential role in CREB's activation and dependent transcription in cardiomyocytes.
Title: Scaffold protein enigma homolog activates CREB whereas a short splice variant prevents CREB activation in cardiomyocytes.
PDLIM5 acts as a scaffold protein by mediated PKCepsilon translocated to the membrane in PMA-induced growth cone collapse.
Title: PDLIM5 mediates PKCε translocation in PMA-induced growth cone collapse.
coordinated and stepwise splicing transitions result in the production of specific ENH transcripts in mature striated muscles.
Title: Splicing transitions of the anchoring protein ENH during striated muscle development.
Neuroligin function was critically dependent on the cytoplasmic tail, but required non-PDZ ligand.
Title: Functional dependence of neuroligin on a new non-PDZ intracellular domain.
Antagonistic splice variants of enigma may play a central role in the adaptive changes of the link between mechanical stress-sensing and signalling occurring during embryonic development and/or heart hypertrophy.
Title: Splice variants of enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy.
Propose a model where ENH1 scaffolds PKD1 to Cacna1c in order to form a signalling complex that regulates the activity of cardiac L-type voltage-gated Ca(2+) channels.
Title: Enigma homolog 1 scaffolds protein kinase D1 to regulate the activity of the cardiac L-type voltage-gated calcium channel.
ENH is a restraining factor of the oncogenic activity of inhibitor of DNA binding 2 proteins in neural tumors
Title: The protein ENH is a cytoplasmic sequestration factor for Id2 in normal and tumor cells from the nervous system.
ENH protein, by interacting specifically with both protein kinase C (PKC)epsilon and the N-type Ca2+ channel, targets a specific PKC to its substrate to form a functional signaling complex.
Title: A PKC epsilon-ENH-channel complex specifically modulates N-type Ca2+ channels.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH132279 (e-PCR)
- UniSTS:215562

RH137910 (e-PCR)
- UniSTS:219997

RH144581 (e-PCR)
- UniSTS:233826

d2rat295 (e-PCR)
- UniSTS:121698

RH129037 (e-PCR)
- UniSTS:212345

AI014024 (e-PCR)
- UniSTS:250255

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables actin binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actinin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables muscle alpha-actinin binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor complex adaptor activity	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell growth involved in cardiac muscle cell development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in heart development	IBA Inferred from Biological aspect of Ancestor more info
involved_in heart development	NAS Non-traceable Author Statement more info	PubMed
involved_in muscle structure development	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of dendritic spine morphogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse assembly	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in adherens junction	IBA Inferred from Biological aspect of Ancestor more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
part_of filamentous actin	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	EXP Inferred from Experiment more info	PubMed
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info
is_active_in stress fiber	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: PDZ and LIM domain protein 5

Names: enigma homolog; enigma-like PDZ and LIM domains protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053326.1 → NP_445778.1 PDZ and LIM domain protein 5

See identical proteins and their annotated locations for NP_445778.1

Status: PROVISIONAL

Source sequence(s)

U48247

UniProtKB/Swiss-Prot

Q62920

UniProtKB/TrEMBL

A0A8I6ARU2, A6HW58

Related

ENSRNOP00000093276.2, ENSRNOT00000114385.2

Conserved Domains (4) summary

cd09453
Location:415 → 466

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 294

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:533 → 587

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

233625478..233793582 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039103056.2 → XP_038958984.1 PDZ and LIM domain protein 5 isoform X14

UniProtKB/TrEMBL

A0A8I6GKL2

Conserved Domains (4) summary

cd09453
Location:314 → 365

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:112 → 181

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:432 → 486

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_063282478.1 → XP_063138548.1 PDZ and LIM domain protein 5 isoform X17

UniProtKB/TrEMBL

A0A8I6GKL2
XM_063282477.1 → XP_063138547.1 PDZ and LIM domain protein 5 isoform X13

UniProtKB/TrEMBL

A0A8I6GKL2
XM_006233419.5 → XP_006233481.1 PDZ and LIM domain protein 5 isoform X16

See identical proteins and their annotated locations for XP_006233481.1

UniProtKB/TrEMBL

A0A8I6GKL2, A6HW57

Related

ENSRNOP00000097787.1, ENSRNOT00000115118.2

Conserved Domains (4) summary

cd09453
Location:306 → 357

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 185

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:424 → 478

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_039103055.2 → XP_038958983.1 PDZ and LIM domain protein 5 isoform X12

UniProtKB/TrEMBL

A0A8I6GKL2

Conserved Domains (4) summary

cd09453
Location:328 → 379

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 195

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:446 → 500

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_006233418.5 → XP_006233480.1 PDZ and LIM domain protein 5 isoform X15

UniProtKB/TrEMBL

A0A8I6GKL2

Conserved Domains (4) summary

cd09453
Location:312 → 363

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 191

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:430 → 484

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_039103054.2 → XP_038958982.1 PDZ and LIM domain protein 5 isoform X11

UniProtKB/TrEMBL

A0A8I6GKL2

Conserved Domains (5) summary

cd09453
Location:334 → 385

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

COG5068
Location:107 → 330

ARG80; Regulator of arginine metabolism and related MADS box-containing transcription factors [Transcription]

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 201

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:452 → 506

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_063282480.1 → XP_063138550.1 PDZ and LIM domain protein 5 isoform X23

UniProtKB/TrEMBL

A0A8I6ADM7
XM_039103053.2 → XP_038958981.1 PDZ and LIM domain protein 5 isoform X10

UniProtKB/TrEMBL

A0A8I6GKL2

Conserved Domains (4) summary

cd09453
Location:339 → 390

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 206

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:457 → 511

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_039103052.2 → XP_038958980.1 PDZ and LIM domain protein 5 isoform X9

UniProtKB/TrEMBL

A0A8I6ARU2

Conserved Domains (6) summary

PLN00049
Location:36 → 142

PLN00049; carboxyl-terminal processing protease; Provisional

cd09453
Location:414 → 465

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

PHA03307
Location:84 → 413

PHA03307; transcriptional regulator ICP4; Provisional

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 304

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:532 → 586

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_063282473.1 → XP_063138543.1 PDZ and LIM domain protein 5 isoform X5
XM_006233416.5 → XP_006233478.1 PDZ and LIM domain protein 5 isoform X4

UniProtKB/TrEMBL

A0A8L2QCL7

Conserved Domains (4) summary

cd09453
Location:437 → 488

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 294

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:555 → 609

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_063282475.1 → XP_063138545.1 PDZ and LIM domain protein 5 isoform X8

UniProtKB/TrEMBL

A0A8I6ARU2
XM_039103050.2 → XP_038958978.1 PDZ and LIM domain protein 5 isoform X3

Related

ENSRNOP00000022387.7, ENSRNOT00000022387.7

Conserved Domains (6) summary

PLN00049
Location:36 → 142

PLN00049; carboxyl-terminal processing protease; Provisional

cd09453
Location:443 → 494

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

PHA03307
Location:63 → 421

PHA03307; transcriptional regulator ICP4; Provisional

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 310

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:561 → 615

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_063282474.1 → XP_063138544.1 PDZ and LIM domain protein 5 isoform X7

UniProtKB/TrEMBL

A0A8I6ARU2
XM_039103049.2 → XP_038958977.1 PDZ and LIM domain protein 5 isoform X2

Conserved Domains (6) summary

PLN00049
Location:36 → 142

PLN00049; carboxyl-terminal processing protease; Provisional

cd09453
Location:447 → 498

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

PHA03378
Location:181 → 446

PHA03378; EBNA-3B; Provisional

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 315

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:565 → 619

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_039103051.2 → XP_038958979.1 PDZ and LIM domain protein 5 isoform X6

UniProtKB/TrEMBL

A0A8I6ARU2

Conserved Domains (6) summary

PLN00049
Location:36 → 142

PLN00049; carboxyl-terminal processing protease; Provisional

cd09453
Location:426 → 477

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

PHA03378
Location:181 → 425

PHA03378; EBNA-3B; Provisional

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 315

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:544 → 598

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_006233412.5 → XP_006233474.1 PDZ and LIM domain protein 5 isoform X1

Conserved Domains (4) summary

cd09453
Location:448 → 499

LIM1_ENH; The first LIM domain of the Enigma Homolog (ENH) family

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 305

DUF4749; Domain of unknown function (DUF4749)

cl02475
Location:566 → 620

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_006233423.2 → XP_006233485.1 PDZ and LIM domain protein 5 isoform X22

Related

ENSRNOP00000094742.2, ENSRNOT00000106810.2

Conserved Domains (2) summary

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:98 → 171

DUF4749; Domain of unknown function (DUF4749)
XM_006233422.2 → XP_006233484.1 PDZ and LIM domain protein 5 isoform X21

UniProtKB/TrEMBL

A0A8I6GJY3

Conserved Domains (2) summary

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 191

DUF4749; Domain of unknown function (DUF4749)
XM_006233421.2 → XP_006233483.1 PDZ and LIM domain protein 5 isoform X20

UniProtKB/TrEMBL

A0A8I6B1G1

Related

ENSRNOP00000091892.2, ENSRNOT00000115772.2

Conserved Domains (2) summary

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:105 → 196

DUF4749; Domain of unknown function (DUF4749)
XM_006233420.4 → XP_006233482.1 PDZ and LIM domain protein 5 isoform X19

UniProtKB/TrEMBL

A0A8I6GJC1, A6HW60

Related

ENSRNOP00000091918.1, ENSRNOT00000109323.2

Conserved Domains (2) summary

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 294

DUF4749; Domain of unknown function (DUF4749)
XM_039103057.1 → XP_038958985.1 PDZ and LIM domain protein 5 isoform X18

UniProtKB/TrEMBL

A6HW60

Conserved Domains (3) summary

PLN00049
Location:36 → 142

PLN00049; carboxyl-terminal processing protease; Provisional

cd00992
Location:10 → 82

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam15936
Location:214 → 315

DUF4749; Domain of unknown function (DUF4749)