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Help

Help can be found in the User's Guide which can be downloaded and is available in the OSIRIS program from the Help menu. Answers to questions and issues can also be found in the FAQ and Troubleshooting guide below, and in the User's Guide.

Optimize OSIRIS

For the best performance using the latest improvements, OSIRIS settings must be optimized. Suggested starting analysis settings for either single source or mixed samples are available in the lab settings [PowerPlex Fusion HID Sole Source] and [GlobalFiler HID Mixture] in the OSIRIS program under the Tools > Lab Settings menu.

Updates

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Attention Mac users with OSIRIS version 2.12.1 and earlier - With newer versions of Mac OSX, sometimes the OSIRIS windows do not appear. This can be remedied by clicking on the OSIRIS icon in the dock (usually at the bottom of the screen) to activate the OSIRIS window. You may need to do this for each new window in OSIRIS. This issue should be resolved in OSIRIS versions 2.12.2 and later.

Macintosh dock

 

Topics

Installing and upgrading OSIRIS

OSIRIS Windows instructions

OSIRIS Macintosh instructions

Attention Mac users with OSIRIS version 2.12.1 and earlier - With newer versions of Mac OSX, sometimes the OSIRIS windows do not appear. This can be remedied by clicking on the OSIRIS icon in the dock (usually at the bottom of the screen) to activate the OSIRIS window. You may need to do this for each new window in OSIRIS. This issue should be resolved in OSIRIS versions 2.12.2 and later.

When upgrading OSIRIS it is generally least confusing to remove old versions. On Windows, run the uninstall.bat file to uninstall MSI file installations, which will maintain your custom lab settings. Windows EXE or ZIP file installations may simply be deleted. Macintosh installations may simply be deleted. Remember to back up any custom lab settings before uninstalling. When installing multiple versions of OSIRIS for evaluation of newer versions, use EXE file installation on Windows. Use care to ensure that analyses are done with the correct version, when multiple versions are installed.

Contact Us

Questions and suggestions are welcome at .

FAQ

  1. Can I analyze .hid and .fsa file types? Yes. The Operating Procedure must be set for the appropriate file type. See General – .fsa and .hid files in Lab Settings.

  2. Can I enter a new kit in OSIRIS? Yes. See Appendix G for instructions. Contact us for help or advice.

  3. Can I use a different ILS? Several different ILS are defined for various kits. Note that some of those are actually one ILS, but use more or fewer of the peaks, in case not all peak data gets collected. If the ILS you use is not defined for the kit you use, see Appendix G for instructions on defining a new ILS. Contact us if you need help.

  4. What do I do if my multiplex has no ladder available? You can either construct a ladder, or you can use a DNA sizing ladder to add a new kit. See Appendix G or contact us for help.

  5. How fast is OSIRIS? OSIRIS should process 2 to 4 samples per second, depending on the number of artifacts in the samples and the speed of your computer/network. So a plate of 98 samples with controls should take 30-60 seconds to analyze. If it runs significantly slower than that on your system, contact us for advice.

  6. How many samples can OSIRIS process at once? OSIRIS will process at least 800 samples in a single batch (single directory). OSIRIS will process an essentially unlimited number of batches in a single processing run. This has been tested with approximately 10,000 samples in 110 directories (plates) in a single analysis that took approximately 1 hour 20 minutes.

  7. How many users can use OSIRIS at the same time? This is limited only by the number of client computers you have and the speed of your network and server. Generally, it should be “lots.” Additionally, each user is creating a separate instance of OSIRIS running on the local client, so multiple users will not affect the speed of operation except as limited by sample data flow across the network to and from the server if the sample data and OSIRIS output is being stored on the server. A single user can run separate instances of OSIRIS on the same computer at the same time (Windows only), allowing different analyses or tasks to be performed simultaneously. Multiple analyses can also be run from a single OSIRIS process (Windows and Macintosh).

  8. Is there any fee for OSIRIS software or use? No. OSIRIS is free and open source. No license or fees are required.

  9. Is OSIRIS source code available? Yes. OSIRIS source code is available at GitHub: https://github.com/ncbi/osiris.

  10. Can I modify OSIRIS, build it into my front end or incorporate it into my own software? OSIRIS is open source and public domain software. You may do anything your heart desires. In any work or product derived from this material, proper attribution to the authors as the source of the software would be appreciated.

Troubleshooting

Problems and Solutions

My analysis failed

My .hid/.fsa analysis failed

I can’t edit the Operating Procedure

Text and notices do not display correctly on a Macintosh

Known issues

Privacy Information

Privacy Statement

OSIRIS is a desktop tool working on your computer with your own data. Your sample profile data is processed on your computer and is not sent over the internet.

For quality monitoring, OSIRIS versions 2.12 and higher send some information about usage statistics back to NCBI. This information is limited to use of the tool, without any sample, profile or batch data that would reveal the context of your analysis.

See Ping_Failure_Message_Glossary.xlsx on the OSIRIS FTP site for a directory of error codes.

Opt-out of Statistics Collection

You can opt-out of statistics collection by performing the following operations:

See the OSIRIS User's Guide for additional details regarding usage statistics and opting out.

OSIRIS web pages

See the NCBI Privacy Policy for the policy that applies to the OSIRIS web pages.  

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