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What's New

New OSIRIS Pull-up paper: Novel Method for Accurately Assessing Pull-up Artifacts in STR Analysis.

Updates

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Version 2.15.1 has been released with important new display features.

Feature Highlights

Recently improved features

 

Topics

 

Releases

Release notes are included with every download and are available on the Download page

Note the Version 2.15.1 bug fix release below

Note the Version 2.13.1 bug fix release below

Note the Version 2.12.2 bug fix release below

Note the Version 2.11.2 bug fix release below

 


Version 2.15.1 Release notes

Version 2.15.1 fixes bugs in the pull-up identification algorithm.

Bug Fixes

Improvements

 


Version 2.15 Release notes

Version 2.15 includes major new features.

New Features

New Graph display options

New Preview Graph toolbar

Improved Pull-Up identification algorithm

Added “Errors” and Details” tabs to the New Analysis window

Added a search box to the Lab Settings window Sample Limits tab

Folder Icon to find Operating Procedures

Updated OSIRIS 2.15 User Guide

• The STR analysis tutorial is updated to demonstrate some of the new display features in OSIRIS 2.15.
• The Fragment analysis tutorial is updated to include both a section on determining the number of data channels in an .fsa/.hid file for fragment analysis and a list of the predefined internal standard markers (ILS) in OSIRIS 2.15.

Bug Fixes

 


Version 2.14 Release notes

Version 2.14 includes major new features.

New Features

Simplified printing

Peak and label colors

Improved analysis robustness

Analysis correction function for artifact peaks

Running OSIRIS from the command line

Added homozygote threshold settings

Removed off ladder allele setting

Changed uncertain pull-up artifact priority

Bug Fixes

 


Version 2.13.1 Release notes

Version 2.13.1 corrects version data in three kit Operating Procedures that prevents settings from being changed.

Bug Fix

 


Version 2.13 Release notes

Version 2.13 includes major new features.

New Features

Fragment Analysis

Flexible export formats

Simultaneous Mixed/Single Source Analysis

Use of Non-Roman and Other Unicode Characters

Identify Lab Settings Folders

Bug Fixes

 


Version 2.12.2 Release notes

Version 2.12.2 fixes bugs in the pull-up identification algorithm and in opening windows on the Macintosh version.

Bug Fixes

 


Version 2.12.1 Release notes

Version 2.12.1 fixes a bug that prevents the COrDIS kit from working.

 


Version 2.12 Release notes

New Features

Improved Artifact Analysis

New Kit /Lab Settings

Usage statistics

OSIRIS version 2.12 collects non-identified usage statistics to help us improve the software. It does not collect information on samples, profiles, batches or information that would reveal the context of the analysis. Users can opt out or disable the statistics reporting. Details are in the Privacy Information section in the appendices of the User’s Guide or see Privacy Information on the Help page.

Bug Fixes

 


Version 2.11.2 Release notes

Version 2.11.2 fixes a bug that could occur under rare conditions, causing a sample to fail if the bug is triggered.

Bug Fix

Baseline bug

Fixed a bug that under rare circumstances, with a particular lab setting combination, could cause intermittent analysis failures of certain samples. When the bug manifested, certain array values were not initialized. The result was an off-scale raw data baseline in one or more channels causing the channel to not analyze. The bug had absolutely no impact on the accuracy of any successful analysis.

The bug fix in version 2.11.2 has been tested with 33 samples and settings that intermittently triggered the bug to demonstrate that the results of analysis with version 2.11.2 is identical to successful analysis with version 2.11.1, including allele calls, peak RFU, peak bp size, artifact calls, etc.

This bug does not affect versions prior to version 2.11 or version 2.12 and higher.

 


Version 2.11.1 Release notes

Version 2.11.1 fixes two bugs that could occur under rare conditions.

Bug Fixes

Pull-up analysis bug Fixed an uncommon bug that in extremely rare circumstances could result in an allele that comigrates with a peak in another channel being called a pull-up in circumstances where the “Min RFU for a peak to be considered as a peak that causes pull-up” in the Sample Limits tab of the Lab Settings is set too high.

ILS peaks with no label Fixed a bug in which ILS peaks that were also called as pull-up were not given a base pair label. While this produced no analysis errors, it could result in display issues.

 


Version 2.11 Release notes

New Features

Improved Display

Improved Artifact Analysis

Improved Baseline and Peak Analysis

Process Quality Control Metrics

Improved Editing

Improved Network Administration

Improved CODIS CMF file exports: Can include comments in the Sample Comments field of the Plate Template in the ABI Genetic Analyzer Data Collection Software that OSIRIS can export to the CODIS CMF Comment field.

Improved ladder analysis: Improved robustness of bi-allelic loci, such as Amelogenin. Reduces artifacts.

New Kit / Lab Settings

Added example Lab Settings for Sole Source and Mixed samples

PowerPlex Fusion HID Sole Source and GlobalFiler HIG Mixture lab default settings include all of OSIRIS novel features and updates and can serve as a starting point for laboratories to optimize their settings for sole source and potential mixed samples. Both can be modified for use with .fsa files, and the settings can be copied to other kits. Both will need to be modified for lab’s own analytical thresholds and desired peak sensitivity.

Bug Fixes

Pull-up analysis bug

Fixed an uncommon bug that in extremely rare circumstances could result in an allele that comigrates with a peak in another channel being called a pull-up. This bug was fixed by making better use of peaks that did not comigrate with a peak in the pull-up channel, or that comigrate with raw data above the noise level for the channel.

ILS peaks with no label

Fixed a bug in which ILS peaks that were also called as pull-up were not given a base pair label. While this produced no analysis errors, it could result in display issues.

Fixed minor bugs that did not impact accuracy

 

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