TOM70 protein channel TOM70 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TOM70
Gene description: protein channel TOM70
Primary source: SGD:S000005065
Locus tag: YNL121C
See related: AllianceGenome:SGD:S000005065
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MAS70; MOM72; OMP1
Summary: Enables mitochondrion targeting sequence binding activity. Contributes to protein transmembrane transporter activity. Involved in protein import into mitochondrial matrix and protein insertion into mitochondrial inner membrane. Located in mitochondrion. Is integral component of mitochondrial outer membrane. Part of mitochondrial outer membrane translocase complex. Human ortholog(s) of this gene implicated in congestive heart failure. Orthologous to human TOMM70 (translocase of outer mitochondrial membrane 70). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (398684..400537, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Determinism and contingencies shaped the evolution of mitochondrial protein import.
Title: Determinism and contingencies shaped the evolution of mitochondrial protein import.
Tom70-mediated interaction with internal binding sites improves the import competence of preproteins and increases the efficiency of their translocation into the mitochondrial matrix.
Title: Tom70 enhances mitochondrial preprotein import efficiency by binding to internal targeting sequences.
budding yeast mitochondrial outer membrane proteins that are subunits of the translocase of the outer mitochondrial membrane (Tom70 and Tom 22) and sorting and assembly machinery (Sam37) are required for efficient pretransfer RNA splicing.
Title: From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing.
Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor.
Title: A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.
Tom70 and Djp1 are crucial players in the biogenesis of Mim1.
Title: The role of Djp1 in import of the mitochondrial protein Mim1 demonstrates specificity between a cochaperone and its substrate protein.
The import receptor Tom70, but not its partner Tom20, is in-volved in the initial recognition of the Ugo1 precursor.
Title: Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-dependent import pathway.
The Mim1 complex cooperates with the receptor Tom70 in binding of precursor proteins and promotes their insertion and assembly into the outer membrane.
Title: The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins.
Results suggest that the Tom70 monomer is the functional unit mediating initial chaperone docking and precursor recognition.
Title: Domain organization of the monomeric form of the Tom70 mitochondrial import receptor.
Data Show that Tom70 are anchored in the outer membrane by a single transmembrane alpha-helix, located at the N terminus of Tom70 (signal-anchored) and that Mim1 is required for efficient membrane insertion and assembly of Tom70.
Title: Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors.
These data indicate an unexpected role for Tom70 in recruitment of soluble proteins to the mitochondrial surface, and indicate that Tom71 has a specialized role in Mfb1-mediated mitochondrial morphogenesis.
Title: Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mitochondrion targeting sequence binding	IBA Inferred from Biological aspect of Ancestor more info
enables mitochondrion targeting sequence binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to protein transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein-transporting ATPase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein import into mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein insertion into mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein insertion into mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in protein insertion into mitochondrial inner membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to mitochondrion	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondria-associated endoplasmic reticulum membrane contact site	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	HDA more info	PubMed
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
part_of mitochondrial outer membrane translocase complex	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein channel TOM70

NP_014278.3

Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially-directed proteins, acting as a receptor for incoming precursor proteins; moonlighting role as a transcriptional regulator of mitochondrial proteins; age-dependent reduction in Tom70p results in mitochondrial dysfunction, a hallmark of aging; localizes to mitochondria-ER contact sites; TOM70 has a paralog, TOM71, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

398684..400537 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182959.3 → NP_014278.3 TPA: protein channel TOM70 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014278.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZRW3, B0KZR5, B0KZS4, B0KZU2, B0KZY7, B0KZZ6, B0L005, B0L023, D6W162, P07213

UniProtKB/TrEMBL

A0A8H4BXV7, B3LNX7, B5VQS5, C8ZGB0, G2WLY6, N1NZD1

Conserved Domains (2) summary

TIGR00990
Location:1 → 614

3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)

sd00006
Location:99 → 127

TPR; TPR repeat [structural motif]