CBF1 Cbf1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CBF1
Gene description: Cbf1p
Primary source: SGD:S000003821
Locus tag: YJR060W
See related: AllianceGenome:SGD:S000003821
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CEP1; CPF1
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II-specific DNA-binding transcription factor binding activity; and sequence-specific double-stranded DNA binding activity. Involved in several processes, including chromatin remodeling; ncRNA transcription; and regulation of transcription by RNA polymerase II. Located in kinetochore. Part of Cbf1-Met4-Met28 complex. Human ortholog(s) of this gene implicated in cardiovascular system disease and type 2 diabetes mellitus. Orthologous to human USF1 (upstream transcription factor 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (548759..549814)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.
Title: Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.
Transcription destabilizes centromere function.
Title: Transcription destabilizes centromere function.
Development of Human CBF1-Targeting Single-Stranded DNA Aptamers with Antiangiogenic Activity In Vitro.
Title: Development of Human CBF1-Targeting Single-Stranded DNA Aptamers with Antiangiogenic Activity In Vitro.
Both the binding and dissociation rates of Reb1 and Cbf1 are significantly slower at the nucleosomal sites relative to those for naked DNA, demonstrating that the high affinities are achieved by increasing the dwell time on nucleosomes in order to compensate for reduced binding.
Title: Dissociation rate compensation mechanism for budding yeast pioneer transcription factors.
Cen-RNAs were more abundant in rnh1Delta cells but only in mid-late S phase. However, fork pausing at centromeres was not elevated in rnh1Delta cells but rather was due to centromere-binding proteins, including Cbf1 Strains with increased cen-RNA lost centromere plasmids at elevated rates.
Title: Saccharomyces cerevisiae Centromere RNA Is Negatively Regulated by Cbf1 and Its Unscheduled Synthesis Impacts CenH3 Binding.
we provide the first evidence for a role of USF1 in respiration since it appeared to complement Cbf1 in vivo as determined by respiration phenotypes. In addition, we confirmed USF1 as a substrate of human PAS kinase (hPASK) in vitro Combined, our data supports a model in which Cbf1/USF1 functions to partition glucose toward respiration and away from lipid biogenesis
Title: The Regulation of Cbf1 by PAS Kinase Is a Pivotal Control Point for Lipogenesis vs. Respiration in Saccharomyces cerevisiae.
PSK1 mediated in vivo phosphorylation of Cbf1 at T211/T212 inhibits yeast cell respiration.
Title: A comprehensive protein-protein interactome for yeast PAS kinase 1 reveals direct inhibition of respiration through the phosphorylation of Cbf1.
Cbf1 is required for transcription at centromere DNA.
Title: Endogenous transcription at the centromere facilitates centromere activity in budding yeast.
Data suggest that the binding sites for Cbf1 and CEP3 (CBF3 subunit) are included within the centromeric nucleosome has important implications for models of the centromeric nucleosome and for kinetochore function.
Title: The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere.
Cbf1p binding to the upstream sites was required for the binding of the ISW2 chromatin-remodeling complex to the Ino2p-Ino4p-binding sites on the INO1 promoter
Title: Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to methionine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of inositol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of sulfate assimilation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cbf1-Met4-Met28 complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IPI Inferred from Physical Interaction more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Cbf1p

NP_012594.1

Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

548759..549814

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181718.1 → NP_012594.1 TPA: Cbf1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012594.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWN1, P17106, P17623, Q6Q5N1

UniProtKB/TrEMBL

A6ZQ19, G2WH65

Conserved Domains (1) summary

cd00083
Location:220 → 272

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...