ALE1 lysophospholipid acyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Gene symbol: ALE1
Gene description: lysophospholipid acyltransferase
Primary source: SGD:S000005701
Locus tag: YOR175C
See related: AllianceGenome:SGD:S000005701
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: LCA1; LPT1; SLC4
Summary: Enables 1-acylglycerol-3-phosphate O-acyltransferase activity and 1-acylglycerophosphocholine O-acyltransferase activity. Involved in glycerophospholipid biosynthetic process. Acts upstream of or within phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine. Located in endoplasmic reticulum. Orthologous to several human genes including MBOAT1 (membrane bound O-acyltransferase domain containing 1) and MBOAT2 (membrane bound O-acyltransferase domain containing 2). [provided by Alliance of Genome Resources, Apr 2022]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (659815..661674, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results reveal that ALE1 is involved in acyl chain transfer to the sn-1 position of HHPC in yeast.
Title: The membrane-bound O-acyltransferase Ale1 transfers an acyl moiety to newly synthesized 2-alkyl-sn-glycero-3-phosphocholine in yeast.
D146 and E297 act in conjunction with H382 as nucleophiles that attack the hydroxyl group in lysophospholipids in a general acid/base mechanism in Lpt1.
Title: Saccharomyces cerevisiae lysophospholipid acyltransferase, Lpt1, requires Asp146 and Glu297 for catalysis.
pology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
Title: Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
Identification of LCA1 provides an essential molecular tool for further study of Lands cycle in phosphatidylcholine turnover
Title: The yeast acylglycerol acyltransferase LCA1 is a key component of Lands cycle for phosphatidylcholine turnover.
in concert with Slc1, Lpt1 plays a central role in Phosphatidic acid biosynthesis, which is essential for cell viability.
Title: LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae.
Slc1p and Slc4p may not only be active as 1-acylglycerol-3-phosphate O-acyltransferases but also be involved in fatty acid exchange at the sn-2-position of mature glycerophospholipids.
Title: SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast.
that a previously uncharacterized open reading frame, YOR175C, encodes the major acyl-CoA-dependent lysophosphatidylethanolamine acyltransferase activity in yeast
Title: Identification and characterization of the major lysophosphatidylethanolamine acyltransferase in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 1-acylglycerophosphocholine O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-acylglycerophosphocholine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-acylglycerophosphocholine O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 1-acylglycerophosphoethanolamine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-acylglycerophosphoserine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glycerophospholipid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycerophospholipid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glycerophospholipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid modification	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondria-associated endoplasmic reticulum membrane contact site	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: lysophospholipid acyltransferase

NP_014818.1

EC 2.3.1.23

EC 2.3.1.51

EC 2.3.1.n6

EC 2.3.1.n7

Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

659815..661674 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183594.1 → NP_014818.1 TPA: lysophospholipid acyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014818.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2N1, Q03130, Q08548

UniProtKB/TrEMBL

A6ZP18, C7GN52, C8ZGT6, E9P8E9, N1P3B1

Conserved Domains (1) summary

COG5202
Location:1 → 563

COG5202; Predicted membrane protein [Function unknown]